• Asian Pacific Journal of Cancer Prevention
• /
• 제13권12호
• /
• pp.6073-6076
• /
• 2012

Discovery of anticancer drugs that kill or disable tumor cells in the presence of normal cells without undue toxicity is a potential challenge for therapeutic care. Several papers in the literature have emphasized the potential implications of marine products such as seaweeds which exhibit antitumor activity. Study attempts to screen the antitumor effect of Sargassum sp, against chosen cell lines such as MCF-7 (Breast cancer) and Hep-2 (Liver Cancer). Ethanol extract of Sargassum sp. was concentrated using a Soxhlet apparatus and dissolved in DMSO. In vitro cytotoxic activity of Sargassum sp at various concentrations ($100{\mu}g/ml-300{\mu}g/ml$) screened for antitumor effect against the chosen cell lines using MTT assay (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, a yellow tetrazole). The study documented that the percentage of cell viability has been reduced with increased concentration, as evidenced by cell death. Sargassum sp extract shows potential cytotoxic activity ($P{\leq}0.05$) with $IC_{50}$ of $200{\mu}g/ml$ and $250{\mu}g/ml$ against Hep-2 and MCF-7 cell lines respectively. The ethanol fraction of Sargassum sp induced cell shrinkage, cell membrane blebbing and formation of apoptotic bodies with evidence of bioactive components as profound influencing factors for anti-tumor effects. Further research need to be explored for the successful application of Sargassum sp as a potent therapeutic tool against cancer.

• 한국수산과학회지
• /
• 제4권3_4호
• /
• pp.105-112
• /
• 1971

1970넌 6월부터 1971넌 5월까지 여수 오동도의 2개 조사지점에서 월 2회 조사한 해조 군락은 아래와 같다. 1. 총출현종은 69종으로 녹조류 12종, 갈조류 17종 및 홍조류 40종이며, 조사 장소에 따른 출현종의 뚜렷한 차이는 없었다. 2. 계절별 해조 군락은 대부분 주년 출현종으로 층위가 형성되어 출현종의 변화가 적으나 Ulva pertusa, Enteromorpha sp., Sphacelaria sp., Scytosiphon iomentaria, Colpomenia sinuosa, Sargassum thunber gii, Pterocladia tenuis, Amphiroa ephedrea, Carpopeltis affinis, Gigartina tenera, Polysiphonia yendoi, Chondria crassicaulis, Symphyocladia atiuscula등은 피도의 증감이 많았다. 3. 층위의 출현종은 대체로 변화가 적어, $200\~150cm$층은 Gloiopeltis complanata, G. furcata, Enteromorpha sp.등이고 $150\~100cm$층은 Ulva pertusa, Chondria crassicaulis, Sargassum thunbergii등이며, $100\~50cm$층은 Gigartina tenera, Pterocladia tenuis, Scytosiphcn lomentaria, Polysiphonia yendoi 등이고, 제일 아래층인 50cm 이하에는 Sargassum sagamianum, Cystophyllum sisymbrioides, Gelidium amansii, Acresorium yendoi, Microcladia elegance등으로 층위가 형성되었다. 4. A, B 두 조사지점은 100cm층을 중심으로 아래층에는 차이가 적으나 위층에는 차이가 있어, A지점에는 Gloiopeltis complanata, Corallina pilulifera, Chondria crassicaulis, Sargassum thunbergii, Polysiphonia yendoi 및 Ulva pertusa등으로 층위가 형성되나, B지점에는 Gloiopeltis complanata, G. furcata, Enteromurpha sp. 및 Ulva pertusa등으로 층위가 간단하였다. 5. Taniguti의 해조 군락 분류에 의하면 Myelophycus caespitosus 군단의 Gigartina intermedia-Sargassum sagaminnum군집 (Sargasseto-Gigartinetum Taniguti)에 속한다.

• 생명과학회지
• /
• 제24권3호
• /
• pp.274-283
• /
• 2014

인간 생체 내 산화스트레스는 조직적 손상을 일으켜 당뇨병, 심장혈관계질환, 비만 등의 대사성 질환의 발병에 관여하는 것으로 알려져 있다. 본 연구에서는 질병예방용 천연 보조제 개발의 일환으로 모자반 3종류(Sargassum hemiphyllum, Sargassum thunbergii, Sargassum honeri)의 에탄올 추출물을 이용하여 항산화활성 및 지방생성억제효과를 비교 검토하였다. 항산화활성은 DPPH 라디칼 소거능, 세포 내 ROS 활성 및 NO 함량의 측정을 통해 검토하였다. 지방세포생성억제활성은 3T3L1세포를 이용하여 지방세포의 축적 정도와 $PPAR{\gamma}$ 유전자의 발현 정도를 각각 Oil-Red O 염색법과 RT-PCR로 측정하였다. DPPH 라디칼 소거능 결과, 모자반의 처리에 의해 농도 의존적으로 DPPH 라디칼이 줄어드는 경향을 보였으며, 모자반 중에서 S. hemiphyllum 처리군에서 가장 높은 소거 효과를 볼 수 있었다. MTT assay을 통해 모자반의 에탄올 추출물들이 RAW 264.7 cell에 대한 독성을 보이지 않는 농도에서 세포내 실험을 진행하였다. 세포 내 ROS 소거능의 측정 결과, 농도 의존적으로 DCF 형광도 값이 낮게 나왔으며, 시간이 지남에 따라 형광도 값이 일정하여 모자반의 에탄올 추출물에는 세포 내 ROS 생성을 억제하는 효과가 있음을 알 수 있었다. LPS 처리에 의해 증가한 NO 값은 모자반의 추출물들의 처리에 의해 유의적으로 감소하였으며, 감소 정도는 S. hemiphyllum과 S. thunbergii 처리 군에서 높게 나타났다. 모자반의 추출물들을 3T3-L1지방세포에 유도물질과 함께 처리한 결과 모자반 추출물 중 S. hemiphyllum과 S. thunbergii이 세포 내 지방 축적 및 $PPAR{\gamma}$ 유전자의 발현을 유의적으로 감소시키는 것으로 나타났다. 이상의 결과로부터 모자반 추출물들 중 S. hemiphyllum과 S. thunbergii이 높은 항산화 활성과 지방세포 생성억제 효능을 보유하고 있는 천연소재임을 확인할 수 있었다.

• 대한한방내과학회지
• /
• 제31권2호
• /
• pp.224-241
• /
• 2010

Objectives : The aim of the current study was to investigate the effects of Sargassum (Sargassum pallidum (TURN.) C. AG.; SP) on the experimental colitis induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) in mice. Methods : ICR mice were divided into 7 groups (NOR, CON, $SS50\times5$, $SP20\times3$, $SP50\times3$, $SP20\times5$, $SP50\times5$). TNBS processing was intrarectally applied to all experimental groups on the 3rd experiment day, except the normal group (NOR). For investigating the prophylactic effect, SP at doses of 20 mg/kg ($SP20\times5$) and 50 mg/kg ($SP50\times5$) were orally administered for 5 days. The SP at doses of 20 mg/kg ($SP20\times3$) and 50 mg/kg ($SP50\times3$) were orally administered for 3 days after the colitis induction in order to check the effect of treatment. As a positive control group, sulfasalazine 50 mg/kg ($SS50\times5$) was administrated. Macroscopic findings of epithelial tissue on mice were measured by colon length and macroscopic score. Histologic findings were also checked by crypt cell, epithelial cell, inflammatory cell and edema of submucosa. We measured the ability of SP to inhibit lipid peroxidation and myeloperoxidase activity. We also measured levels of the inflammatory markers, interleukin (IL)-$1\beta$ and cyclooxygenase-2 (COX-2), its transcription factor activation, phospho-NF-${\kappa}B$ (pp65), in the colon by enzyme-linked immunosorbent assay and immunoblot analysis. We measured activation of fecal bacterial enzyme, $\beta$-glucuronidase and degradation activation of fecal glycosaminoglycan (GAG), and hyaluronic acid. Results : Oral administration of SP on mice inhibited TNBS-induced colon shortening and myeloperoxidase activity in the colon of mice as well as IL-$1\beta$ and COX-2 expression. SP also inhibited TNBS-induced lipid peroxidation and pp65 activation in the colon of mice. SP inhibited $\beta$-glucuronidase activation and fecal hyaluronic acid degradation activation as well. Conclusions : SP could be a possible herbal candidate and preventive prebiotic agent for treating inflammatory bowel disease (IBD). Further experiments to differentiate effects of SP on IBD, such as other solutions and extracting times, might be promising.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제11권2호
• /
• pp.160-163
• /
• 2006

A total number of 31 types of seaweed were assessed with regard to their effects on the proliferation of mouse spleen and thymus cells in a culture, using an MTT reduction assay. Acetone:dichloromethane (1:1) extracts of three seaweed plants: Derbesia marina, Sargassum sp., and Hisikia fuziformis, exhibited significantly positive effects on the survival of mouse spleen and thymus cells in vitro. The acetone:dichloromethane (1:1) extracts of Sargassum sp., in particular, much more potent effects on thymus cell activation than did any of the other types of seaweed. However, the methanol extracts of Sargassum ringgoldianium and Chondrus crispus exerted a stimulatory influence only on the proliferation of mouse spleen cells, whereas the methanol extracts of Grateloupia lanceolata exhibited significant cell proliferation properties in both spleen and thymus cells.

• 한국균학회지
• /
• 제44권4호
• /
• pp.259-262
• /
• 2016

Arthrinium (Ascomycota, Apiosporaceae) is a major marine fungal genus. Three Arthrinium species were reported previously, but not fully described. We isolated the two species A. arundinis and A. saccharicola from Sargassum sp. brown algae in Jeju Island, Korea. These species have not been previously reported in Korea. We described these species in detail and performed a phylogenetic analysis based on the nucleotide sequences of the EF1-${\alpha}$ and ${\beta}$-tubulin genes.

• 한국양식학회:학술대회논문집
• /
• 한국양식학회 2004년도 수산관련학회 공동학술대회 발표요지집
• /
• pp.228-229
• /
• 2004

• 한국수산과학회지
• /
• 제43권4호
• /
• pp.345-350
• /
• 2010

We studied the organisms attached to Sargassum beds in Gamak Bay from November 2007 to June 2008. The water temperature ranged from 3.3 to $28.7^{\circ}C$ and salinity from 30.6 to 33.8 psu. Eighteen species $143,432\;ind./m^2$ were sampled during study periods. The biomass of the Sargassum beds ranged from a highest of $196.08\;g\;dw/m^2$ in March to a lowest of $0\;g\;dw/m^2$ in July. The major dominant species were Barleeia angustata ($134,430\;ind./m^2$), Caprellidae sp.($2,443\;ind./m^2$) and Gammaridae sp.($4,201\;ind./m^2$). The ecological indices of diversity, evenness, and richness were 0.11-0.86, 0.04-0.39, and 0.74-1.06, respectively.

• Ocean and Polar Research
• /
• 제25권1호
• /
• pp.129-132
• /
• 2003

The crude extracts of marine algae were screened for tyrosinase-inhibitory and radical scavenging effects. Among the samples tested, Symphyocladia latiuscula and Gloiopeltis furcata were found to be the most effective in DPPH radical scavenging test while Gigatina tenella, Sargassum thunbergii, and Sargassum sp. were moderately active. For the inhibition against mushroom tyrosinase, Symphyocladia latiuscula and Sargassum confusum showed the strongest inhibition. Codium adhaerens, Corallina pilulifera, Carpopeltis cornea, Halymenia acuminata, Hizikia fuziformis, Porphyra suborbiculata, and Enteromorpha linza exhibited mild inhibitory potency.

• 한국어병학회지
• /
• 제18권2호
• /
• pp.147-156
• /
• 2005

본 연구에서는 주요 어병세균인 Staphylococcus sp., Streptococcus sp., E. tarda 및 V. anguillarum에 대한 30 종의 해조류 추출물의 항균활성을 검색하였다. 그 결과, C. officinalis, D. simplex, G. furcata, G. lanceolata 및 G. turuturu의 경우는 그람 양성균인 Staphylococcus sp.에 대하여, 그리고 S. thunbergii 및 P. morrowii 는 그람 음성균인 E. tarda와 V. anguillarum에 대하여 항균활성을 나타냄을 밝혔다. 그 중, 가장 우수한 항균활성을 나타낸 P. morrowii의 항균활성분획물인 90% MeOH 분획물은 E. tarda의 증식을 매우 효과적으로 저해하며, MIC는 1 mg/ml으로 측정되었다. 뿐만 아니라, 다양한 pH 조건하에서도 항균활성이 유지되었으며 분획물의 주요활성 성분은 할로겐 함유 phenol계 화합물일 것으로 추정된다.