• 약학회지
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• 제38권5호
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• pp.579-585
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• 1994

Antitumor activity was tested by administration of reaction mixture of green onion extract and chitin to mice bearing sarcoma-180 cells. An intraperitoneal injection of mixture(20 mg/kg/day) to mice Have an 52% inhibition of tumor growth. Inhibition of tumor growth was found to be dose-dependent. When eighty miligrams of the mixture were administered, the weight of tumor was reduced significantly. HPLC analysis indicated the mixture was composed of N-acetyl-D-glucosamine, N-acetylchitobiose and N-acetylchitotriose.

• 한국임상수의학회지
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• 제24권3호
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• pp.367-371
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• 2007

Artemisia capillaris THUNB is a perennial herb that belongs to the family Compositae spp and the most common plant among the various herbal folk remedies used in treatment of abdominal pain, hepatitis, chronic liver disease, jaundice and coughing in Korea. This experiment was conducted to investigate cytotoxic effects of Artemisia capillaris extracts on the Hepa-1c1c7 and Sarcoma 180 cancer cells on in vitro experimental tests. On in vitro tests using MTT assay and SRB assay, the extracts showed prominent cytotoxic effects on the two kinds of cancer cell lines, respectively. Antihumor effects appeared in the concentration of over $250{\mu}g/mL$ of both ethanol and ethyl acetate extract, over $500{\mu}g/mL$ of methanol extract, over $5000{\mu}g/mL$ in water extract and over 50% cytotoxicity on the Hepa-1c1c7 and Sarcoma 180. The results suggest that Artemisia capillaris extracts have prominent cyotoxic effects on the cancer cell lines Hepa-1c1c7 and Sarcoma 180.

• 한방안이비인후피부과학회지
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• 제19권1호
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• pp.55-64
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• 2006

Objective : The purpose of this study was to investigate effect of Gungguitakli-San(GTS) on the anti-tumor, immunocytes. Methods : This study estimated the proliferation of L1210 and S-180 cell lines, mouse splenocytes and thymocytes in vitro, and estimated the proliferation of L1210 cell, S-180 cell, thymocytes and splenocytes and body weight in S-180 cells-transplanted mice. The cytotoxicity and proliferation of cells were tested using a colorimetric tetrazoliun assay(M1T assay). Results : The results of this study were obtained as follow ; 1. GTS was significantly increased in the proliferation of thymocytes and splenocytes In vitro. 2. GTS was significantly showed cytotoxicity on the L1210 cell lines and 8-180 cell lines in vitro. 3. GTS was significantly showed cytotoxicity on the L1210 cell lines in vivo. 4. GTS was significantly increased in the weight of mice and decreased weight of sarcoma, in S-180 cells transplanted mice. 5. GTS was significantly increased in the period of survive, in S-180 cells transplanted mice. Conclusions : The author thought that GTS had action of anti-cancer by becoming immunocytes activity and by cytotoxicity of cancer cells.

• 한국식품영양과학회지
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• 제31권4호
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• pp.691-695
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• 2002

한국산 솔잎을 가압.압착하여 얻은 수액을 증류한 솔잎 수액 증류액의 각종 암세포주에 대한 in uitro 세포독성을 시료액 대비 10배, 20배, 40배 희석군과 대조군에 대해 XTT법으로 실험한 결과, 쥐 백혈병 세포주인 L1210에 대해서는 76~89%, 쥐 육종암세포인 sarcoma 180에 대해서는 61~90%의 세포성장 억제효과를 나타내었다. 또한 인체의 monocyte-like cancer cell인 U937에 대해서는 56~81%, 인체 유방암 세포주인 T47D와 MDA-MB-231에서는 각기 12%, 또 다른 유방암 세포주인 MH7A에서는 64%, 인체 간암 세포주인 SNU-354에 대해서는 72%의 높은 세포 증식 억제효과를 나타내었다. 이로써 본 연구 시료인 솔잎 수액 증류액은 쥐 백혈병 세포주인 L1210, 쥐 육종암세포인 sarcoma 180, 인체 monocyte-like cancer cell인 U937, 인체 유방암 세포주인 MH7A, 인체 간암 세포주인 SNU-354에 대해 강한 세포독성을 갖는 것을 알 수 있었다. 이와 함께 솔잎 수액 증류액의 암세포에 대한 독성효과는 솔잎의 처리과정에 따라 다를 수 있으며, 또한 동일한 솔잎 수액증류액의 농도에서도 암세포주 종류에 따라 세포독성정도가 다름을 알 수 있었다. 따라서 최적 투여 농도와 적용 암세포주를 찾을 경우 새로운 항암제로 개발될 수 있음을 제시하였다.

• 생명과학회지
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• 제18권9호
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• pp.1290-1298
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• 2008

송이는 담자균문, 주름버섯목, 송이과에 속하며 맛이 좋은 식용 버섯으로 예로부터 사람의 위궤양이나 위암의 치료효과는 물론 생쥐의 Sarcoma 180과 Ehrlich Sarcoma에도 효과가 있다고 알려져 있다. 본 연구에서는 송이의 자실체로부터 중성염용액, 열수 및 메탄올을 이용하여 조다당류를 추출하고 ICR mice에 주사하여 항암 및 면역증강 효과를 조사하였다. NIH3T3, Sarcoma 180, HepG2, HT-29 등의 암세포에 대한 독성을 조사한 결과 각각의 암세포는 2 mg/ml의 조다당류 농도에서는 세포독성을 나타내지 않았다. Sarcoma 180이 접종된 ICR mouse에 자실체에서 추출한 각각의 조다당류를 투여한 실험군은 대조군에 비해 평균수명이 각각 23.4$\sim$37.2% 연장되었다. 메탄올과 열수로 추출한 조다당류를 0.2$\sim$0.5 mg/ml의 농도로 투여한 실험군 생쥐의 B 임파구 alkaline phosphatase 활성은 대조군에 비해 각각 2.17$\sim$11.9배의 증가하였다. 중성염추출 조다당류를 50 mg/kg body weight의 농도로 투여한 생쥐의 총 복강 세포 수와 백혈구의 수는 대조군에 비하여 각각 6배와 1.5배 증가하였다. 따라서 송이의 자실체에서 추출한 조다당류는 생쥐의 Sarcoma 180에 대해 항암작용 및 면역증강 작용을 나타내는 것으로 확인되었다.

• Archives of Pharmacal Research
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• 제20권4호
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• pp.368-371
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• 1997

In an effort to increase of the antitumor activity of 2(S)-$2^{I}$,$5^{I}$-trihydroxy-7, 8-dimethoxyflavanone isolated from Scutellaria indica, we synthesized its analogues, II, III and IV. They showed potent cytotoxicity in vitro against cancer cell lines, L1210, K562 and A549. On the basis of $ED_50$ values against the cancer cell lines, III exhibited about 2-7 times stronger activity than I against various cell lines. We tested the antitumor activity of the analogues against Sarcoma 180 cells in vivo and evaluated the structure-activity relationship. The antitumor activity appeared to be related to the hydrogen bond between carbonyl group at C-4 and hydroxyl group at C-5, in contrast to cytotoxic action.

• Applied Biological Chemistry
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• 제38권4호
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• pp.364-369
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• 1995

목단피의 mouse 복수암에 대한 항암성을 다른 생약제의 항암성과 비교하여 연구하였다. 천연생약제(목단피, 주목, 울금, 인경쓱, 여정실, 맥문동) methanol 추출물을 hexane, chloroform ($CHCl_3$), ethylacetate (EtOAc), butanol (BuOH)로 fractionation하여 mouse leukemia L1210 cell과 Sarcoma 180 (S-180) cell에 강한 독성을 나타낸 fraction에 대해 mouse 복수암 억제실험을 실시하였다. 복수형 종양세포 S-180을 ICR mouse (male, $6{\sim}7$주령, $23g{\pm}3g$, 처리당 7마리)의 복부에 주사 ($1{\times}10^{6}$ cells/0.1 ml PBS)한 1일 후 부터 10% DMSO에 용해한 시료 ($30{\mu}g/g$ body weight)를 매일 0.1ml씩 10일간 주사하고 수명연장 효과와 몸무게의 변화를 조사하였다. 처리 fraction 중에서 목단피의 EtOAc fraction이 가장 강한 항암성을 보였는데, 수명연장에서는 대조구의 17.2일 (100%)에 비하여 28.7일로서 167%로 연장되었으며, 체중 증가율도 대조구보다 낮았다 (p<0.05). 목단피의 농도별 (5, 10, 30, $60{\mu}g/g$ body weight) 항암효과는 $30{\mu}g$에서 가장 높았고, $60{\mu}g$처리구 에서는 독성이 나타났다. 목단피의 EtOAc fraction으로부터 GC-MS에 의해 잠정적으로 동정된 2-methoxyphenol, 1-(4-hydroxy-3- methoxyphenyl)-ethanone, 8-methyl-2,4(1H,3H)-pteridinedione, 2,5-furandicarboxylic dimethyl ester 가 mouse 복수암 억제에 관련이 있는 주요 화합물로 추정된다.

• Nutrition Research and Practice
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• 제4권3호
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• pp.177-182
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• 2010

The Chaga mushroom (Inonotus obliquus) has been used in folk medicine to treat cancers. However, limited information exists on the underlying anticancer effects of the major component of I. obliquus in vivo. We hypothesize that the pure compounds ($3{\beta}$-hydroxy-lanosta-8,24-dien-21-al, inotodiol and lanosterol, respectively) separated from I. obliquus would inhibit tumor growth in Balbc/c mice bearing Sarcoma-180 cells (S-180) in vivo and growth of human carcinoma cells in vitro. To test this hypothesis, the growth inhibition of each subfraction isolated from I. obliquus on human carcinoma cell lines (lung carcinoma A-549 cells, stomach adenocarcinoma AGS cells, breast adenocarcinoma MCF-7 cells, and cervical adenocarcinoma HeLa cells) was tested in vitro. Then, after S-180 implantation, the mice were fed a normal chow supplemented with 0, 0.1 or 0.2 mg of subfraction 1, 2 or 3 per mouse per day. All of the subfractions isolated from I. obliquus showed significant cytotoxic activity against the selected cancer cell lines in vitro. Subfraction 1 was more active than subfraction 2 and subfraction 3 against the A549, AGS and MCF-7 cancer cell lines in vitro. In in vivo results, subfraction 1 isolated from I. obliquus at concentrations of 0.1 and 0.2 mg/mouse per day significantly decreased tumor volume by 23.96% and 33.71%, respectively, as compared with the control. Subfractions 2 and 3 also significantly inhibited tumor growth in mice bearing S-180 as compared with the control mouse tumor. Subfraction 1 isolated from I. obliquus showed greater inhibition of tumor growth than subfractions 2 and 3, which agrees well with the in vitro results. The results suggest that I. obliquus and its compounds in these subfractions isolated from I. obliquus could be used as natural anticancer ingredients in the food and/or pharmaceutical industry.

• Biomolecules & Therapeutics
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• 제7권1호
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• pp.29-34
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• 1999

The cellular growth inhibition of 20 natural products was screened using SRB (sulforhodamine B) assay against 4 human cancer cell lines(SNU-1, SNU-C$_{4}$, Hep3B, Kato III). Ethanol extracts of Salvia miltiorrhiza, Saussurea lappa and Chelidonium majus showed potent anticancer activity among them, and further, it was fractionated into methylene chloride, hexane and methanol. Methylene chloride and methanol fraction of Salvia radix showed significant inhibitory activity against 4 human cancer cell lines. The effect of Salvia miltiorrhiza on anticancer activity in vitro models was evaluated with methylene chloride fraction of Salvia miltiorrhiza. Life span of ICR mice implanted with sarcoma-180 was increased by 40-61% and BDF$^{1}$ mice implanted with L1210 was increased by 66-89% upon intraperitoneal administration with methylene chloride fraction of Salvia miltiorrhiza. Based on these result, we suggested that Salvia miltiorrhiza showed anticancer activity on the in vivo and in vitro models

• 한방안이비인후피부과학회지
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• 제19권1호
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• pp.93-102
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• 2006

Objective : Gamisibgi-San was a drug that treated carbuncle and cellulitis. So, the purpose of this Study was to investigate effects of Gamisibgi-San on the anti-cancer and proliferation of immunocytes. Materials and Method : We used Gamisibgi-San extract(GMSGS) with freeze-dried, 8wks-old male mice and cancer cell lines(L1210, S-180) for this Study. The cytotoxicity and proliferation of cells wat tested using a colorimetric tetrazoliun assay(MIT assay). Results and Conclusion : The results of this Study were obtained as follow ; 1. GMSGS was significantly showed cytotoxicity on the L1210 cell lines and S-180 cell lines. 2. GMSGS was significantly increased in the proliferation of thymocytes and splenocytes in vitro. 3. GMSGS was significantly decreased in the proliferation of L1210 cells in L1210 cells transplanted mice. 4. GMSGS was significantly decreased in the Weight of Sarcoma in S-180 cells transplanted mice. 5. GMSGS was significantly increased in the Period of Survive in S-180 cells transplanted mice. The present author thought that GMSGS had action of anti-cancer by becoming immunocytes activity(proliferation of thymocytes and splenocytes).