Browse > Article
http://dx.doi.org/10.3746/jkfn.2002.31.4.691

Cytotoxic Effect of the Distilled Pine-Needle Extracts on Several Cancer Cell Lines in vitro  

Chung, Young-Jin (Dept. of Food and Nutrition, Chungnam National University)
Bae, Myung-Won (Dept. of Food and Nutrition, Chungnam National University)
Chung, Myoung-Il (Dept. of Food and Nutrition, Chungnam National University)
Lee, Ji-Seon (Dept. of Pharmacy, Chungnam National University)
Chung, Kyeong-Soo (Dept. of Pharmacy, Chungnam National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.31, no.4, 2002 , pp. 691-695 More about this Journal
Abstract
This study was performed to examine the cytotoxic effects of the distilled pine-needle extracts against several cancer cell lines. First, cell lines including mice leukemic cancer cell line (L1210), sarcoma 180 and human monocyte-like cancer cells (U937) were tested using XTT methods in uitro. Pine-needle extracts were prepared by pressing the pine needles and distilling it at below 98$^{\circ}C$ and then added to the growth medium in a final dilution of 10, 20, and 40 times. Growth of three kinds of cancer cells was significantly inhibited by more than 50% with the addition of the extracts. Fifty six to seventy six % of inhibition was shown with the 40 times dilution of the extracts. Greater inhibition was achieved with the 20 times dilution (81~90%) and the 10 times dilution (77~89%) of the extracts. Next, other human cancer cell lines including 3 kinds of breast cancer cell lines (T47D, MDA-MB-231 and MW7A) and one hepatoma cell line (SNU-354) were tested with the 20 times dilution of the extract. T47D and MDA-MB-231 cell lines showed lower inhibition (12%) with the addition of the extract. However, MH7A and SNU-354 cell lines showed 64% and 72% inhibition with the extract, respectively. These results suggest that the distilled pine-needle extracts have strong cytotoxic effect on certain cancer cell lines and the intensity of the effect may vary depending on the process of the pine needle.
Keywords
distilled pine-needle extracts; cytotoxicity; cancer cell lines; XTT assay;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Chung KS, Yoon KD, Kwon DJ, Hong SS, Choi SY. 1997. Cytotoxicity testing of fermented soybean products with various tumour cells using MTT assay. Kor J Appl Microbiol Biotechnol 25: 477-482.
2 Scudiero DA, Shoemaker RH, Paull KD, Monks A, Tierney S, Nofziger TH, Currens MJ, Seniff D, Boyd MR. 1988. Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res 48: 4927-4833.
3 Shin HK. 1997. The development of functional food and research trends. Food Sci and Industry 30: 2-13.
4 Yoon SU. 1997. Pine tree and naturopathy. Academy book, Seoul. p 40-43.
5 Murakami A, Ohigashi H, Koshimizu K. 1996. Anti-tumor promotion with food phytochemicals: a strategy for cancer chemoprevention. Biosci Biotech Biochem 60: 1-8.   DOI   ScienceOn
6 Kobayashi N, Unten S, Kakuta H, Komatsu N, Fujimaki M, Satoh K, Aratsu C, Nakashima H, Kikuchi H, Ochiai K, Sakagami H. 2001. Diverse biological activities of healthy foods. In vivo 15: 17-23.
7 Choe M. 1991. Dietary fats and cancer. J Korean Soc Food Nutr 20: 513-518.   과학기술학회마을
8 Choi JH, Kim DI, Park SH, Kim DW, Lee JH, Kim HS. 2001. Investigation of anti-aging effect and determination of chemical structure of pine needle (Pinus densiflora) through animal experiment. IV. Effects of ethyl acetate fraction on oxygen radicals and their scavenger enzymes in brain of SD rats. Korean J Gerontol 11: 7-13.
9 Park JG, Hyun JW, Lim KH, Shin JE. 1993. Antineoplastic effect of extracts from traditional medicinal plants. Kor J Pharmacogn 24: 223-230.   과학기술학회마을
10 Park SH, Rhee IJ. 1994. Effect of Selaginella tamariscina on U937 cytotoxicity. J Korean Soc Food Nutr 23: 799-804.
11 21. Moon JJ, Han YB, Kim JS, 1993. Studies on antitumor effects of pine needles, Pinus densiflora Sieb. et Zucc. Korean Vet Res 33: 701-710.
12 Newmark HL. 1996. Plant phenolics as potential cancer prevention agents. Adv Exp Med Biol 401: 25-34.   DOI
13 Kang YH, Park YK, Oh SR, Moon KD. 1995. Studies on the physiological functionality of pine needle and mugwort extracts. Korea J Food Sci Technol 27: 978-984.   과학기술학회마을
14 Park CS. 1998. Antibacterial activity of ethanol extract of pine needle against pathogenic bacteria. Korean J Postharvest Sci Technol 5: 380-385.   과학기술학회마을
15 Kuk JH, Ma SJ, Park KH. 1997. Isolation and characterization of benzoic acid with antimicrobial activity from needle of Pinus densiflora. Korean J Food Sci Technol 29: 204-210   과학기술학회마을
16 Choi EJ, Lee E, Rhim TJ, Cha BC, Park HJ. 1997. Antimicrobial activities of pine needle (Pinus densiflora) extract. Korean J Appl Microbiol Biotechnol 25: 293-297.   과학기술학회마을
17 Chan EW, Cheng SC, Sin FW. 2001. Triptolide induced cytotoxic effects on human promyelocytic leukemia, T cell lymphoma and human hepatocellular carcinoma cell lines. Toxicology Letters 122: 81-87.   DOI   ScienceOn
18 Steinmetz KA, Potter JD. 1991. Vegetable, fruit and cancer II mechanisms. Cancer Causes Control 2: 427-442.   DOI   ScienceOn
19 Kim EJ, Choi KP, Ham SS, Kang HY. 1998. Inhibitory effect of pine needle extracts on the chemically induced mutagenicity. Korean J Food Sci Technol 30: 450-455.   과학기술학회마을
20 Choi JH, Kim DI, Park SH, Kim DW, Lee JH, Kim HS. 2001. Investigation of anti-aging effect and determination of chemical structure of pine needle (Pinus densiflora) through animal experiment. III. Effects of butanol fraction on oxygen radicals and their scavenger enzymes in brain of SD rats. Korean J Gerontol 11: 7-13.
21 Lee IR, Song JY, Lee YS. 1992. Cytotoxicity of folkloric medicine and human cancer cells. Kor J Pharmacogn 23: 132-136.   과학기술학회마을
22 Dragsted LO, Strube M, Larsen JC. 1993. Cancer-protective factor in fruits and vegetables: biochemical and biological background. Pharmacol Toxicol 72: 116-135.
23 Seo YJ. 1997. Chemoprevention of cancer with foods. Food Sci and Industry 30: 59-63.
24 Moon SH, Kim KH, Park KY. 1996. Antitumor effect of persimmon leaves in vivo using Sarcoma-180 cells. J Korean Soc Food Sci Nutr 25: 865-870.   과학기술학회마을
25 Kim SM, Cho YS, Kim EJ, Bae MJ, Han JP, Lee SH, Sung SK. 1998. Effect of hot water extracts of Salvia militiorrhiza Bge., Prunus persica Stokes, Angelica gigas Nakai and Pinus strobus on lipid oxidation. J Korean Soc Food Sci Nutr 27: 399-405.   과학기술학회마을
26 Azuine MA, Goswami UC, Kayal JJ, Bhide SV. 1992. Antimutagenic and anticarcinogenic effects of carotenoids and dietary palm oil. Nutr Cancer 17: 287-295.   DOI   ScienceOn
27 Kong Z, Liu Z, Ding B. 1995. Study on antimutagenic effect of pine needle extract. Mutat Res 347: 101-104.   DOI   ScienceOn
28 Kim EJ, Jung SW, Choi KP, Ham SS. 1998. Cytotoxic effect of the pine needle extracts. Korean J Food Sci Technol 30: 213-217.   과학기술학회마을