• Title/Summary/Keyword: Sample pre-treatment

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Evaluation of Biological Activities of Wheat Sprouts with Different Extraction Solvents (새싹 밀의 추출용매에 따른 생리활성 평가)

  • Kim, Hyun Young;Seo, Hye-Young;Seo, Woo Duck;Lee, Mi Ja;Ham, Hyeonmi
    • The Korean Journal of Food And Nutrition
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    • v.32 no.6
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    • pp.636-642
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    • 2019
  • The purpose of this study was to examine biological activities, including total contents of polyphenol, antioxidant activities, inhibitory activities of tyrosinase, and protective effect against oxidative stress in the HepG2 cells of ethanol extracts from wheat sprout. The antioxidant activity of extracts was determined by ABTS and DPPH radical scavenging activities. Ethanol extracts were tested using different ethanol concentrations (0%, 30%, 50%, 80% and 95%, respectively). The highest amount of total polyphenol was extracted by 50% and 80% ethanol which was 26.3 and 26.8 mg gallic acid equivalents/g sample, respectively. High levels of ABTS and DPPH radical scavenging activity were found in 50% ethanol (26.7 and 15.0 mg TEAC/g sample, respectively) and 80% (24.3 and 16.1 mg TEAC/g sample, respectively) ethanol extracts. Also, 50% and 80% ethanol extracts indicated higher inhibitory activities of tyrosinase compared with other extracts. In the cell-based assay, pre-treatment of the HepG2 cells with wheat sprout extracts prevented the cell damage induced by TBHP (tert-butyl hydroperoxide). The results of this study indicate that wheat sprout has significantly higher diverse biological activities and apparently has significant health benefits.

Preparation and Characterization of Wood Polymer Composite by a Twin Screw Extrusion (이축 압출공정을 이용한 Wood Polymer Composite의 제조 및 특성 분석)

  • Lee, Jong-Hyeok;Lee, Byung-Gab;Park, Ki-Hun;Bang, Dae-Suk;Jhee, Kwang-Hwan;Sin, Min-Cheol
    • Elastomers and Composites
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    • v.46 no.3
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    • pp.211-217
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    • 2011
  • Wood Polymer Composite (WPC) has attracted a great deal of attention in environmental industries due to renewable resources, processability, excellent physical properties and logging regulations for application to housing units and engineering construction materials. In this study, commercial WPCs were prepared by using a modular intermeshing co-rotating twin screw extruder. The effect of three main factors such as wood flour contents, coupling agent concentrations and pre-treatment of wood flour on the properties of WPCs was extensively investigated. It was found that tensile strength and thermal stability were decreased with increasing wood flour contents whereas the water absorption was increased. Addition of maleic anhydride grafted polypropylene (PP-g-MA) into WPC exhibited better physical properties. On the contrary, the water absorption was slightly decreased with PP-g-MA. Finally the sample, which was prepared with pre-treated wood flour, represented the highest tensile strength. However, the water absorption of the sample was increased due to the transition of crystalline structure of cellulose.

The Effects of Assertiveness Traning and Value Clarification Training on Nurse's Conflict and Conflict Management Mode (주장훈련과 가치명료화훈련이 간호사의 갈등정도와 갈등관리 양식에 미치는 효과)

  • Park, Sang-Yeon
    • Journal of muscle and joint health
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    • v.2 no.1
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    • pp.41-72
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    • 1995
  • The purpose of this study is to examine the effects of assertiveness training and value clarification training on nurse's conflict and conflict management mode. Fifty seven registered nurses participated in the study ; they were employed by three general hospital located in Daegu, Korea. The study employs two treatment groups. The assertiveness training group consisted of subjects who participated in 90-120 minutes sessions of assertiveness training nine times over five weeks. The other treatment group, was adiministed nine, 90-120 minutes sessions of value clarification during the same period. For the control group, nursing subjects were appointed the training after five weeks. Pre-test evaluation were administered to all subjects in three groups prior to one week of the treatment. Role conflict Inventory-general(RCI-G) and Communication Conflict Inventory-general (CCI-G) measure nurse's conflict management mode. Post-test evaluation were administered to all subjects in three groups two weeks after the last session by Role Conflict Inventory-Specific(RCI-S), Communication Conflict Inventory-Specific (CCI-S), Management Model-Specific(CMMI-S). The analysis of variance(ANOVA) and covariance(ANCOVA) on gain scores were running the SPSS program. In order to test statistical differences among mean scores of the scales obtained after treatment, multiple comparisons were carried out by Turkey method. Conclusions obtained from the results are as follows. 1. The assertiveness training and the value clarification training were effective in decreasing the nurse's role conflict. The value clarification was more effective than the assertiveness training in decreasing the nurse's role conflict. 2. Both assertiveness training and value clarification training were effective in decreasing nurse's communication conflict. There was, however, no differences between assertiveness training and value clarification training in decreasing the nurse's communication conflict. 3. The assertiveness training and the value clarification training were quite effective in compromizing and collaborating conflict management mode, to reducing the withdrawl and accomodate, force and accomodate conflict management mode to conflict. There was no difference in the effectiveness of assertiveness training and value clarification. In assessing the effects of the treatments, this study employed different measurements. It is unclear whether the measurement affected the test results. It is worth conducting a further test using the same measurements. The results of future studies can be compared with those of this study. The homogeneity of the control group and treatment group is questionable. Futher studies may employ homogeneous sample group to evaluate whether the sample characteristics bias the test results. Assertiveness training or value clarification training for nurses can be utilized in nursing intervention.

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Study on the Removal of Fluorescent Whitening Agent by Pretreatment Ozone Oxidation for MBR Process Application (MBR 공정 적용을 위한 전처리 오존산화에 의한 형광증백제 제거 연구)

  • Choi, Jang-Seung;Ryu, Seung-Han;Shin, Dong-Hun;Lee, Jae-Hun;Lee, Soo-Chol;Kim, Sung-Gi;Ryu, Jae-Young;Shin, Won-Sik;Lee, Seul-Ki;Park, Min-Soo
    • Textile Coloration and Finishing
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    • v.29 no.1
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    • pp.11-17
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    • 2017
  • In this study, ozone oxidation experiment was carried out for the removal of fluorescent whitening agent which is widely used in textile dyeing and paper industry. The stilbene fluorescent whitening agent has been industrialized since the earliest, and the amount of current production is the highest. Due to the characteristics of the fluorescent whitening agent that can not be removed by conventional wastewater treatment methods, the fluorescent whitening agent in wastewater treatment has difficulty in using as recycled water in the process. Pre-treatment ozone oxidation experiment was conducted prior to the introduction of Membrane Bio Reactor(MBR) treatment process by converting biodegradable materials into biodegradable materials. The removal efficiencies of fluorescent whitening agents, a diaminostilbenedisulfonic acid derivative by ozone oxidation were evaluated by $UV_{254}$ Scan, $COD_{Mn}$, T-N and color using a synthetic wastewater sample ($COD_{Mn}=433.0mg/{\ell}$) and paper and paper mill wastewater ($COD_{Mn}=157.2mg/{\ell}$).

Effects of 8 weeks administration of Korean Panax ginseng extract on the mood and cognitive performance of healthy individuals

  • L., Reay J.;B., Scholey A.;O., Kennedy D.
    • Journal of Ginseng Research
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    • v.31 no.1
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    • pp.34-43
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    • 2007
  • Background: Previous research has suggested that single doses of a standardised Panax ginseng extract can decrease fasted blood-glucose levels and modulate cognitive performance in healthy young volunteers. The latter has generally been seen in terms of improved secondary memory performance. However, both the cognitive effects of chronic administration of ginseng and the potential modulation of working memory have received comparatively little research attention. Aims: The current double-blind, placebo-controlled, balanced cross-over study investigated the effects of 8-weeks administration of Korean ginseng extract (200 mg) on cognitive performance, gluco-regulatory parameters and ratings of subjective mood and 'quality of life'. Methods: 'Eighteen healthy young participants were assessed pre-dose and 3 hours post-dose on the mornings of Day 1, Day 29 and Day 57 of 8 week treatment regimens of both placebo and ginseng. A four-week placebo wash-out separated the treatment phases. Each assessment included the Cognitive Drug Research battery, computerised working memory tasks, and Bond-Lader mood scales. The WHO Quality of Life scale (WHOQOL-BREF) was completed once per visit. Gluco-regulatory parameters were assessed with assays of blood glucose, insulin and HbA1c. Results: Data from the 16 participants that completed the study showed that there were no significant, acute treatment related differences on Day 1 of treatment, or in gluco-regulatory parameters throughout the study. However, time related performance improvements were evident following chronic administration of ginseng on the '3-Back' and 'Corsi-block' computerised working memory tasks. Ginseng was also associated with an improved score on the 'social relations' subscale of the WHOQOL-100, and a significant shift on the 'calm' factor of the Bond-Lader mood scales (from calm/relaxed towards excited/tense). Conclusion: The results of the current study suggest that Korean ginseng extract can modulate working memory performance and subjective ratings of 'quality of life' and mood. Replication with a larger sample size may further elucidate the actions of this product.

Inactivation of Mycobacteria by Radicals from Non-Thermal Plasma Jet

  • Lee, Chaebok;Subhadra, Bindu;Choi, Hei-Gwon;Suh, Hyun-Woo;Uhm, Han. S;Kim, Hwa-Jung
    • Journal of Microbiology and Biotechnology
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    • v.29 no.9
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    • pp.1401-1411
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    • 2019
  • Mycobacterial cell walls comprise thick and diverse lipids and glycolipids that act as a permeability barrier to antibiotics or other chemical agents. The use of OH radicals from a non-thermal plasma jet (NTPJ) for the inactivation of mycobacteria in aqueous solution was adopted as a novel approach. Addition of water vapor in a nitrogen plasma jet generated OH radicals, which converted to hydrogen peroxide ($H_2O_2$) that inactivated non-pathogenic Mycobacterium smegmatis and pathogenic Mycobacterium tuberculosis H37Rv. A stable plasma plume was obtained from a nitrogen plasma jet with 1.91 W of power, killing Escherichia coli and mycobacteria effectively, whereas addition of catalase decreased the effects of the former. Mycobacteria were more resistant than E. coli to NTPJ treatment. Plasma treatment enhanced intracellular ROS production and upregulation of genes related to ROS stress responses (thiolrelated oxidoreductases, such as SseA and DoxX, and ferric uptake regulator furA). Morphological changes of M. smegmatis and M. tuberculosis H37Rv were observed after 5 min treatment with $N_2+H_2O$ plasma, but not of pre-incubated sample with catalase. This finding indicates that the bactericidal efficacy of NTPJ is related to the toxicity of OH and $H_2O_2$ radicals in cells. Therefore, our study suggests that NTPJ treatment may effectively control pulmonary infections caused by M. tuberculosis and nontuberculous mycobacteria (NTM) such as M. avium or M. abscessus in water.

Bone changes in the mandibular incisors after orthodontic correction of dental crowding without extraction: A cone-beam computed tomographic evaluation

  • Valerio, Claudia Scigliano;Cardoso, Claudia Assuncao e Alves;Arauujo, Eustaquio Afonso;Zenobio, Elton Goncalves;Manzi, Flavio Ricardo
    • Imaging Science in Dentistry
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    • v.51 no.2
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    • pp.155-165
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    • 2021
  • Purpose: This study aimed to evaluate changes of the alveolar bone and interdental bone septum of the mandibular incisors through cone-beam computed tomography (CBCT) after orthodontic treatment of mandibular dental crowding without dental extraction. Materials and Methods: The sample consisted of 64 CBCT images(32 pre-treatment and 32 post-treatment) from 32 adult patients with class I malocclusion and an average age of 23.0±3.9 years. The width and height of the alveolar bone and interdental septum, the distance between the cementoenamel junction (CEJ) and the facial and lingual bone crests, and the inclination of the mandibular incisors were measured. Results: The distance between the CEJ and the marginal bone crest on the facial side increased significantly (P<0.05). An increased distance between the CEJ and the bone crest on the facial and lingual sides showed a correlation with the irregularity index (P<0.05); however, no significant association was observed with increasing mandibular incisor inclination (P>0.05). The change in the distance between the CEJ and the marginal bone crest on the facial side was correlated significantly with bone septum height(P<0.05). Conclusion: Bone dehiscence developed during the treatment of crowding without extraction only on the incisors' facial side. Increasing proclination of the mandibular incisor was not correlated with bone dehiscence. The degree of dental crowding assessed through the irregularity index was associated with the risk of developing bone dehiscence. The interdental septum reflected facial marginal bone loss in the mandibular incisors.

Can Sodium Citrate Effectively Improve Olfactory Function in Non-Conductive Olfactory Dysfunction? (Sodium Citrate가 효과적으로 비전도성 후각장애에 치료효과를 보일 수 있을지에 대한 문헌 고찰)

  • Kim, Subin;Kang, Haram;Jin, Ho Jun;Hwang, Se Hwan
    • Korean Journal of Otorhinolaryngology-Head and Neck Surgery
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    • v.62 no.2
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    • pp.75-81
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    • 2019
  • The objective of this study was to perform a systematic review of the literature for application of intranasal sodium citrate in the patients with olfactory dysfunction to help determine the sodium citrate treatments for this condition. Two authors independently searched the data base (Medline, Scopus, and the Cochrane database) for relevant studies from inception to January 2018. Included studies were randomized controlled studies published in English comparing topical sodium citrate application (treatment group) with saline (control group) in patients who had olfactory dysfunction. Outcomes of interest included the change of olfactory identification and threshold during 2 hours post-treatment. Three studies were enrolled in the meta-analysis. Compared with control group, treatment group did not increase posttreatment score of olfactory identification [standardized mean difference (SMD)=-0.03; 95% confidence interval (CI)=-0.29-0.24; I2=0%] and olfactory threshold (SMD=0.18; 95% CI=-0.09-0.45; I2=0%) significantly. In the degree of pre-post improvement of two outcomes, although treatment group statistically showed the significant improvement in olfactory threshold (SMD=0.30; 95% CI=0.05-0.55; I2=17%), the clinical significance of this outcome was meaningless. Similarly, there was no significant difference in olfactory identification between two groups (SMD=0.17; 95% CI=-0.11-0.45; I2=22%). Unlike the recent favorable results, our summated results presented the uselessness for the local application of sodium citrate in improving patient's olfactory function. However, we also had some limitation such as small sample size and inconsistent application methods. Therefore, larger trials and standardized methodology are needed to reach more stronger and exact results.

The Non-Destructive Determination of Heavy Metals in Welding Fume by EDXRF (EDXRF에 의한 용접흄 중의 중금속의 비파괴 정량)

  • Park, Seunghyun;Jeong, Jee Yeon;Ryoo, Jang Jin;Lee, Naroo;Yu, Il Je;Song, Kyung Seuk;Lee, Yong Hag;Han, Jeong Hee;Kim, Sung Jin;Park, Jung sun;Chung, Ho Keun
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.11 no.3
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    • pp.229-234
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    • 2001
  • The EDXRF(Energy Dispersive X-ray Fluorescence Spectrometer) technique was applied to the determination of heavy metals in welding fume. The EDXRF method designed in this study was a non-destructive analysis method. Samples were analyzed directly by EDXRF without any pre-treatment such as digestion and dilution. The samples used to evaluate this method were laboratory samples exposed in a chamber connected with a welding fume generator. The samples were first analyzed using a non-destructive EDXRF method. The samples subsequently were analyzed using AAS method to verify accuray of the EDXRF method. The purpose of this study was to evaluate the possibility of the non-destructive analysis of heavy metals in welding fume by EDXRF. The results of this study were as follow: 1.When the samples were collected under the open-face sampling condition, a surface distribution of welding fume particles on sample filters was uniform, which made non-destructive analysis possible. 2. The method was statistically evaluated according to the NIOSH(National Institute for Occupational Safety and Health) and HSE(Health and Safety Executive) method. 3. The overall precision of the EDXRF method Was calculated at 3.45 % for Cr, 2.57 % for Fe and 3.78 % for Mn as relative standard deviation(RSD), respectively. The limits of detection were calculated at $0.46{\mu}g$/sample for Cr, $0.20{\mu}g$/sample for Fe and $1.14{\mu}g$/sample for Mn, respectively. 4. A comparison between the results of Cr, Fe, Mn analyzed by EDXRF and AAS was made in order to assess the accuracy of EDXRF method. The correlation coefficient between the results of EDXRF and AAS was 0.9985 for Cr, 0.9995 for Fe and 0.9982 for Mn, respectively. The overall uncertainty was determined to be ${\pm}12.31%$, 8.64 % and 11.91 % for Cr, Fe and Mn, respectively. In conclusion, this study showed that Cr, Fe, Mn in welding fume were successfully analyzed by the EDXRF without any sample pre-treatment such as digestion and dilution and a good correlation between the results of EDXRF and AAS was obtained. It was thus possible to use the EDXRF technique as an analysis method of working environment samples. The EDXRF method was an efficient method in a non-destructive analysis of heavy metals in welding fume.

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A Study on Pre-school Teachers' Perception of 7 Guidelines of Safety Education: Using IPA Methodology (유아교사가 인식하는 유치원 안전교육 표준안에 대한 연구 : IPA기법을 중심으로)

  • Kang, Min-Jung;Han, Sun-Ah
    • The Journal of the Korea Contents Association
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    • v.17 no.6
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    • pp.661-671
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    • 2017
  • The purpose of this study is to compare and analyze the difference between the importance perceived by pre-school teachers and their actual performance in regards to the 7 Guidelines of Safety Education set out by the Ministry of Education, and through the analysis, to identify the items that need priority support and those that need long-term improvement efforts for exemplary safety education for young children. For this, mean average and standard deviation were calculated and paired sample t-test conducted, and IPA(Importance-Performance Analysis) matrix method was performed to analyze the priority and long-term support items and improvement needs. The study results show differences in all areas and all items in the 7 Guidelines of Safety Education by the MoE in the pre-school teachers' perceived importance and actual performance. Areas and items that are identified to be in need of priority support for exemplary safety education in pre-schools; 2 items under 'Violence and Personal Safety' area and I item under 'Substance and Internet Addictions' area. Areas and items that are identified to be in need of long-term improvement efforts are; 6 items under 'First Aid and Emergency Treatment' area, 1 item under 'Violence and Personal Safety' area, 3 items under 'Workplace Safety Culture' area, 3 items under 'Substance and Internet Addictions' area and 1 item under 'Disaster and Safety' area.