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http://dx.doi.org/10.9799/ksfan.2019.32.6.636

Evaluation of Biological Activities of Wheat Sprouts with Different Extraction Solvents  

Kim, Hyun Young (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration)
Seo, Hye-Young (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration)
Seo, Woo Duck (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration)
Lee, Mi Ja (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration)
Ham, Hyeonmi (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration)
Publication Information
The Korean Journal of Food And Nutrition / v.32, no.6, 2019 , pp. 636-642 More about this Journal
Abstract
The purpose of this study was to examine biological activities, including total contents of polyphenol, antioxidant activities, inhibitory activities of tyrosinase, and protective effect against oxidative stress in the HepG2 cells of ethanol extracts from wheat sprout. The antioxidant activity of extracts was determined by ABTS and DPPH radical scavenging activities. Ethanol extracts were tested using different ethanol concentrations (0%, 30%, 50%, 80% and 95%, respectively). The highest amount of total polyphenol was extracted by 50% and 80% ethanol which was 26.3 and 26.8 mg gallic acid equivalents/g sample, respectively. High levels of ABTS and DPPH radical scavenging activity were found in 50% ethanol (26.7 and 15.0 mg TEAC/g sample, respectively) and 80% (24.3 and 16.1 mg TEAC/g sample, respectively) ethanol extracts. Also, 50% and 80% ethanol extracts indicated higher inhibitory activities of tyrosinase compared with other extracts. In the cell-based assay, pre-treatment of the HepG2 cells with wheat sprout extracts prevented the cell damage induced by TBHP (tert-butyl hydroperoxide). The results of this study indicate that wheat sprout has significantly higher diverse biological activities and apparently has significant health benefits.
Keywords
wheat sprout; biological activity; polyphenolics; antioxidants;
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1 Ben-Arye E, Goldin E, Wengrower D, Stamper A, Kohn R, Berry E. 2002. Wheat grass juice in the treatment of active distal ulcerative colitis: A randomized double-blind placebocontrolled trial. Scand J Gastroenterol 37:444-449   DOI
2 Biesalski HK. 2002. Free radical theory of aging. Curr Opin Clin Nutr Metab Care 5:5-10   DOI
3 Bonfili L, Amici M, Cecarini V, Cuccioloni M, Tacconi R, Angeletti M, Fioretti E, Keller JN, Eleuteri AM. 2009. Wheat sprout extract-induced apoptosis in human cancer cells by proteasomes modulation. Biochimie 91:1131-1144   DOI
4 Byun MW, Jo C, Jeon TW, Hong CH. 2004. Effects of gamma irradiation on color characteristics and biological activities of extracts of Lonicera japonica (Japanese honeysuckle) with methanol and acetone. LWT-Food Sci Technol 37:29-33   DOI
5 Choi MH, Kim KH, Yook HS. 2018. Antioxidant activity of fermented Kaempferia parviflora and inhibitory action against tyrosinase and elastase. J Korean Soc Food Sci Nutr 47:1076-1084   DOI
6 Choi Y, Jeong HS, Lee J. 2007. Antioxidant activity of methanolic extracts from some grains consumed in Korea. Food Chem 103:130-138   DOI
7 Donkor ON, Stojanovska L, Ginn P, Ashton J, Vasiljevic T. 2012. Germinated grains-sources of bioactive compounds. Food Chem 135:950-959   DOI
8 Ha TY. 2006. Development of functional food materials for healthy life. Korean Soc Crop Sci 51:26-39
9 Halliwell B. 1996. Antioxidants in human health and disease. Annu Rev Nutr 16:33-50   DOI
10 Han JH, Moon HK, Chung SK, Kang WW. 2015. Comparison of physiological activities of radish bud (Raphanus sativus L.) according to extraction solvent and sprouting period. J Korean Soc Food Sci Nutr 44:549-556   DOI
11 Kim YS, Kim R, Moon JH, Ji JR, Choi HD, Park YK. 2009. Optimization of extraction conditions of polyphenolic compounds from apple pomace by response surface methodology. Korean J Food Sci Technol 41:245-250
12 Jeong EY, Sung BK, Song HY, Yang JY, Kim DK, Lee HS. 2010. Antioxidative and antimicrobial activities of active materials derived from Triticum aestivum sprouts. J Korean Soc Appl Biol Chem 53:519-524   DOI
13 Kim EY, Baik IH, Kim JH, Kim SR, Rhyu MR. 2004. Screening of the antioxidant activity of some medicinal plants. Korean J Food Sci Technol 36:333-338
14 Kim JS, Lee JY, Park KT, An BJ, Lee SH, Cho YJ. 2013. The biological activity from Prunella vulgaris extracts. Korean J Food Preserv 20:234-241   DOI
15 Kong SY, Choi YM, Lee SM, Lee JS. 2008. Antioxidant compounds and antioxidant activities of the methanolic extracts from milling fractions of black rice. J Korean Soc Food Sci Nutr 37:815-819   DOI
16 Kubo I, Kinst-Hori I, Yokokawa Y. 1994. Tyrosinase inhibitors from Anacardium occidentale fruits. J Nat Prod 57:545-551   DOI
17 Lee YS, Choi JB, Joo EY, Kim NW. 2007. Antioxidative activities and tyrosinase inhibition of water extracts from Ailanthus altissima. J Korean Soc Food Sci Nutr 36:1113-1119   DOI
18 Lee SH, Lee YM, Lee HS, Kim DK. 2009. Anti-oxidative and anti-hyperglycemia effects of Triticum aestivum wheat sprout water extracts on the streptozotocin-induced diabetic mice. Korean J Pharmacogn 40:408-414
19 Lee SH, Lim SW, Lee YM, Hur JM, Lee HS, Kim DK. 2010. Anti-diabetic effects of Triticum aestivum L. water extracts in db/db mice as an animal model of diabetes mellitus type II. Korean J Pharmacogn 41:282-288
20 Lee SH, Lim SW, Lee YM, Seo JW, Kim DK. 2011. Inhibitory effects of Triticum aestivum L. extracts on liver lipid accumulation in high fat-fed mice. Korean J Pharmacogn 42:309-316
21 Sharma OP, Bhat TK. 2009. DPPH antioxidant assay revisited. Food Chem 113:1202-1205   DOI
22 Nagaoka H. 2005. Treatment of germinated wheat to increase levels of GABA and IP6 catalyzed by endogenous enzymes. Biotechnol Prog 21:405-410   DOI
23 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med 26:1231-1237   DOI
24 Ryu EM, Seo GW, Kee KH, Shin HJ. 2013. Effect of ethanolic extract from wheat sprout on hair growth of C57BL/6 mouse. Korean J Aesthet Cosmetol 11:1051-1057
25 Velioglu YS, Mazza G, Gao L, Oomah BD. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 46:4113-4117   DOI
26 Shewry PR. 2009. Wheat. J Exp Bot 60:1537-1553   DOI
27 Solano F, Briganti S, Picardo M, Ghanem G. 2006. Hypopigmenting agents: An updated review on biological, chemical and clinical aspects. Pigment Cell Res 19:550-571   DOI
28 Stadtman ER, Berlett BS. 1998. Reactive oxygen-mediated protein oxidation in aging and disease. Drug Metab Rev 30:225-243   DOI
29 Yang B, Zhao M, Jiang Y. 2008. Optimization of tyrosinase inhibition activity of ultrasonic-extracted polysaccharides from longan fruit pericarp. Food Chem 110:294-300   DOI
30 Yu SC, Oh TJ. 2016. Antioxidant activities and antimicrobial effects of extracts from Auricularia auricula-judae. J Korean Soc Food Sci Nutr 45:327-332   DOI
31 Ahn MJ, Yuk HJ, Lee HY, Hwang CE, Jeong YS, Hong SY, Kwon OK, Kang SS, Kim HR, Park DS, Cho KM. 2015. Effect of the enhanced biological activities and reduced bitter taste of bitter melon (Momordica charantia L.) by roasting. J Agric Life Sci 49:107-119   DOI