• Title/Summary/Keyword: Sample handling

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Aerosol filtration and electrostatic properties of electrospun nanofiber air filters (전기방사 나노섬유 에어필터의 정전기적 특성 및 에어로졸 여과특성)

  • Park, Hyun-Seol;Lim, Koung Soo
    • Particle and aerosol research
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    • v.5 no.2
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    • pp.71-81
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    • 2009
  • In this study, Nylon 6,6 electrospun (ES) nanofiber filter media were prepared at various spinning conditions. The ES filters tested had no intrinsic electrical charges. The ES filters were triboelectrically charged in the course of filter sample handling, and the charge was drastically decayed in a few hours. On the other hand, the corona charged melt blown filter media showed a permanent electrical charge. The electrical charge state of the ES filters was also examined by comparing collection efficiencies of ES filters for uncharged and charged aerosol particles.

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A survey on Hospital Infection (병원 감염관리에 대한 실태조사 -간호수기를 중심으로 -)

  • 이선옥
    • Journal of Korean Academy of Nursing
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    • v.23 no.3
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    • pp.325-338
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    • 1993
  • This study was conducted to evaluate the methods used in nursing procedures for infection control. Skilled nursing procedures are related to prevention of hospital infection. The sample consisted of 301 nurses’ response to the questionnaire. Data were collected from Feb. to March, 1993 from 35 hospitals located in five major cities. The findings of the study are as follows : 1) Rotatively unsafe nursing procedures were reported for preperation of IV therapy, change of IV site and aseptic dressing on IV site, hand wash-ing and use of paper towels, use of sterile urine bottle with indwelling urinary catheter, management of dressing cart, disinfection of transfer forcep, ambu respirator, laryngoscope, humidifier and handling of incubator. 2) Relatively safe nursing procedures were reported for management of suction tube, marking for contaminated materials and waste collection. The rate of participation in education programs for infection control by the nurses was higher than in other research results. 3) Further study on procedures for IV site in-fusion and care of dressing cart are recommended.

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Classification for Imbalanced Breast Cancer Dataset Using Resampling Methods

  • Hana Babiker, Nassar
    • International Journal of Computer Science & Network Security
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    • v.23 no.1
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    • pp.89-95
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    • 2023
  • Analyzing breast cancer patient files is becoming an exciting area of medical information analysis, especially with the increasing number of patient files. In this paper, breast cancer data is collected from Khartoum state hospital, and the dataset is classified into recurrence and no recurrence. The data is imbalanced, meaning that one of the two classes have more sample than the other. Many pre-processing techniques are applied to classify this imbalanced data, resampling, attribute selection, and handling missing values, and then different classifiers models are built. In the first experiment, five classifiers (ANN, REP TREE, SVM, and J48) are used, and in the second experiment, meta-learning algorithms (Bagging, Boosting, and Random subspace). Finally, the ensemble model is used. The best result was obtained from the ensemble model (Boosting with J48) with the highest accuracy 95.2797% among all the algorithms, followed by Bagging with J48(90.559%) and random subspace with J48(84.2657%). The breast cancer imbalanced dataset was classified into recurrence, and no recurrence with different classified algorithms and the best result was obtained from the ensemble model.

Probing Intracluster Light of 10 Galaxy Clustersat z >1 with Deep HST WFC3/IR Imaging Data

  • Joo, Hyungjin;Jee, M. James;Ko, Jongwan
    • The Bulletin of The Korean Astronomical Society
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    • v.46 no.2
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    • pp.42.2-42.2
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    • 2021
  • Intraclusterlight (ICL) is diffuse light from stars that are bound to the clusterpotential, not to individual member galaxies. Understanding the formationmechanism of ICL provides critical information on the assembly and evolution ofthe galaxy cluster. Although there exist several competing models, the dominantproduction mechanism is still in dispute. The ICL measurement between z=1 and 2strongly constrains the formation scenario of the ICL because the epoch is whenthe first mature clusters begin to appear. However, the number of high-redshiftICL studies is small mainly because of observational challenges. In this study, based on deep HST WFC3/IR data, we measured ICL of 10 galaxy clusters atredshift beyond unity, which nearly doubles the sample size in this redshiftregime. With careful handling of systematics including object masking, skyestimation, flatfielding, dwarf galaxy contamination, etc., we quantified thetotal amount of ICL, measured the color profile, and examined the transitionbetween BCG and ICL.

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Anti-stress Effects of Natural Products from Jeju Island in Zebrafish (제브라피쉬에서의 제주도 천연추출물의 항스트레스 효과)

  • Lee, Jeongwon;Lee, Seungheon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.85-85
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    • 2019
  • Objective: In this study, the anti-stress effects of extract of Hydrangeae Dulcis Folium (EHDF) or ethalonic extract of Opuntiaficus-indica (EOF) of natural extracts from Jeju Island were investigated. Methods: We performed measurement of whole-body cortisol level and behavioral experiments including the novel tank test (NTT) or the open field test (OFT) to assess stress responses in zebrafish. To induce physical stress, we used the net handling stress (NHS). Fish were treated with EOF or EHDF for 6 min before they were exposed to stress. And then, we sacrificed fish for collecting body fluid from whole-body or conducted behavioural tests, including novel tank test and open field test, were evaluated to observe anxiety-like behaviours and locomotion. We used the cortisol enzyme-linked immunoassay kit to measure the amount of cortisol in each zebrafish sample. Results: The results indicate that increased anxiety-like behaviours in novel tank test and open field test under stress were prevented by treatment with both EOF and EHDF (P < 0.05). Moreover, compared with the unstressed group, which was not treated with NHS, the whole-body cortisol level was significantly increased by treatment with NHS. Compared with the NHS-treated stressed control group, pre-treatment with each EHDF and EOF for 6 min significantly prevented the NHS-increased whole-body cortisol level (P < 0.05). Conclusions: In conclusion these results suggest that both EOF and EHDF pretreatment may prevent stress responses and that its mechanism of action may be related to its positive effects on cortisol release.

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Efficient GPU Framework for Adaptive and Continuous Signed Distance Field Construction, and Its Applications

  • Kim, Jong-Hyun
    • Journal of the Korea Society of Computer and Information
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    • v.27 no.3
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    • pp.63-69
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    • 2022
  • In this paper, we propose a new GPU-based framework for quickly calculating adaptive and continuous SDF(Signed distance fields), and examine cases related to rendering/collision processing using them. The quadtree constructed from the triangle mesh is transferred to the GPU memory, and the Euclidean distance to the triangle is processed in parallel for each thread by using it to find the shortest continuous distance without discontinuity in the adaptive grid space. In this process, it is shown through experiments that the cut-off view of the adaptive distance field, the distance value inquiry at a specific location, real-time raytracing, and collision handling can be performed quickly and efficiently. Using the proposed method, the adaptive sign distance field can be calculated quickly in about 1 second even on a high polygon mesh, so it is a method that can be fully utilized not only for rigid bodies but also for deformable bodies. It shows the stability of the algorithm through various experimental results whether it can accurately sample and represent distance values in various models.

Evaluation of an Automated Liquid-Handling System with Fixed Pipetting Tips in Radioimmunoassay (고정용 팁을 사용하는 Automated Liquid-Handling System의 RIA적용의 평가)

  • Shin, Sun-Young;Kim, Hee-Sun;Lee, Hyun-Ju;Jang, Hyun-Young;Woo, Jae-Ryong;Lee, Ho-Young
    • The Korean Journal of Nuclear Medicine Technology
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    • v.14 no.2
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    • pp.190-196
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    • 2010
  • Purpose: This study was undertaken to investigate the feasibility of using automated liquid-handling systems equipped with reusable fixed tips in Radioimmunoassays and to demonstrate that the use of an automated pipetting instrument can decrease in the typical daily workload. Materials and Methods: The precisions of the automated pipettor and the manual pipettor were determined gravimetrically (n=30). A total of 30 specimens for HBs Ag were repeatedly pipetted (three replicates) with the automated pipettor and then retested. PSA samples were simultaneously pipetted with either the automated pipettor or the manual pipettor and then analyzed (n=40). Sample carryover test assessed for CA19-9, AFP and HCG when the automated pipettor was used. Pipetting speed of the automated pipettor and the manual pipettor were compared by evaluation of each workload. Results: The precisions coefficients of variation (CV) were 2.1% for the automated pipettor and 1.6% for manual pipettor. The mean cpm and CV for each group of replicates were 41,203 cpm and 3.7% for HBs Ag positive specimens, and 99 cpm and 7.9% for HBs Ag negative specimens, respectively. PSA results showed no significant differences between automated pipettor and manual pipettor (p=0.15, r=0.999). Carryover for CA19-9, AFP and HCG analytes was <0.1 ppm or below the assay limit of detection. Pipetting speed was significantly improved by using the automated instrument. Conclusion: There was no evidence that the use of an automated pipettor adversely affected any of the performance characteristics of the assay. Indeed, routine use of the Tecan automated pipettor has resulted in a decrease in the typical daily workload.

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Modified Recursive PC (수정된 반복 주성분 분석 기법에 대한 연구)

  • Kim, Dong-Gyu;Kim, Ah-Hyoun;Kim, Hyun-Joong
    • The Korean Journal of Applied Statistics
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    • v.24 no.5
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    • pp.963-977
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    • 2011
  • PCA(Principal Component Analysis) is a well-studied statistical technique and an important tool for handling multivariate data. Although many algorithms exist for PCA, most of them are unsuitable for real time applications or high dimensional problems. Since it is desirable to avoid extensive matrix operations in such cases, alternative solutions are required to calculate the eigenvalues and eigenvectors of the sample covariance matrix. Erdogmus et al. (2004) proposed Recursive PCA(RPCA), which is a fast adaptive on-line solution for PCA, based on the first order perturbation theory. It facilitates the real-time implementation of PCA by recursively approximating updated eigenvalues and eigenvectors. However, the performance of the RPCA method becomes questionable as the size of newly-added data increases. In this paper, we modified the RPCA method by taking advantage of the mathematical relation of eigenvalues and eigenvectors of sample covariance matrix. We compared the performance of the proposed algorithm with that of RPCA, and found that the accuracy of the proposed method remarkably improved.

Qualification Test of ROCSAT -2 Image Processing System

  • Liu, Cynthia;Lin, Po-Ting;Chen, Hong-Yu;Lee, Yong-Yao;Kao, Ricky;Wu, An-Ming
    • Proceedings of the KSRS Conference
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    • 2003.11a
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    • pp.1197-1199
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    • 2003
  • ROCSAT-2 mission is to daily image over Taiwan and the surrounding area for disaster monitoring, land use, and ocean surveillance during the 5-year mission lifetime. The satellite will be launched in December 2003 into its mission orbit, which is selected as a 14 rev/day repetitive Sun-synchronous orbit descending over (120 deg E, 24 deg N) and 9:45 a.m. over the equator with the minimum eccentricity. National Space Program Office (NSPO) is developing a ROCSAT-2 Image Processing System (IPS), which aims to provide real-time high quality image data for ROCSAT-2 mission. A simulated ROCSAT-2 image, based on Level 1B QuickBird Data, is generated for IPS verification. The test image is comprised of one panchromatic data and four multispectral data. The qualification process consists of four procedures: (a) QuickBird image processing, (b) generation of simulated ROCSAT-2 image in Generic Raw Level Data (GERALD) format, (c) ROCSAT-2 image processing, and (d) geometric error analysis. QuickBird standard photogrammetric parameters of a camera that models the imaging and optical system is used to calculate the latitude and longitude of each line and sample. The backward (inverse model) approach is applied to find the relationship between geodetic coordinate system (latitude, longitude) and image coordinate system (line, sample). The bilinear resampling method is used to generate the test image. Ground control points are used to evaluate the error for data processing. The data processing contains various coordinate system transformations using attitude quaternion and orbit elements. Through the qualification test process, it is verified that the IPS is capable of handling high-resolution image data with the accuracy of Level 2 processing within 500 m.

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Development of an Automatic PCR System Combined with Magnetic Bead-based Viral RNA Concentration and Extraction

  • MinJi Choi;Won Chang Cho;Seung Wook Chung;Daehong Kim;Il-Hoon Cho
    • Biomedical Science Letters
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    • v.29 no.4
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    • pp.363-370
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    • 2023
  • Human respiratory viral infections such as COVID-19 are highly contagious, so continuous management of airborne viruses is essential. In particular, indoor air monitoring is necessary because the risk of infection increases in poorly ventilated indoors. However, the current method of detecting airborne viruses requires a lot of time from sample collection to confirmation of results. In this study, we proposed a system that can monitor airborne viruses in real time to solve the deficiency of the present method. Air samples were collected in liquid form through a bio sampler, in which case the virus is present in low concentrations. To detect viruses from low-concentration samples, viral RNA was concentrated and extracted using silica-magnetic beads. RNA binds to silica under certain conditions, and by repeating this binding reaction, bulk samples collected from the air can be concentrated. After concentration and extraction, viral RNA is specifically detected through real-time qPCR (quantitative polymerase chain reaction). In addition, based on liquid handling technology, we have developed an automatic machine that automatically performs the entire testing process and can be easily used even by non-experts. To evaluate the system, we performed air sample collection and automated testing using bacteriophage MS2 as a model virus. As a result, the air-collected samples concentrated by 45 times then initial volume, and the detection sensitivity of PCR also confirmed a corresponding improvement.