• Korean Journal of Medicinal Crop Science
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• v.24 no.4
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• pp.271-276
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• 2016

Background: In the present study, we established an HPLC (high performance liquid chromatography)-analysis method for the determination of marker compounds as a part of the material standardization for the development of health-functional foods from Salvia plebeia R. Br. extract. Methods and Results: The quantitative determination method of hispidulin as a marker compound was optimized by HPLC analysis using a YMC hydrosphere C18 column with a gradient elution system. This method was validated using specificity, linearity, accuracy, and precision tests. It showed a high linearity in the calibration curve with a coefficient of correlation ($r^2$) of 0.999995. The method was fully validated, and was sensitive, with the limit of detection (LOD) at $0.09{\mu}g{\cdot}m{\ell}^{-1}$ and limit of quantification (LOQ) at $0.27{\mu}g{\cdot}m{\ell}^{-1}$. The relative standard deviation (RSD) values of the data from intra- and inter-day precision were 0.05 - 0.22% and 0.32 - 0.42%, respectively, and the intra- and inter-day accuracy of hispidulin were 99.5 - 102.3% and 98.8 - 101.5%, respectively. The average content of hispidulin in Salvia plebeia R. Br. extract was $3.945mg{\cdot}g^{-1}$ (0.39%). Conclusions: These results suggest that the developed HPLC method is very efficient, and that it could contribute to the quality control of Salvia plebeia R. Br. extracts as a functional ingredient in health functional foods.

• Korean Journal of Medicinal Crop Science
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• v.23 no.3
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• pp.245-252
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• 2015

This study was carried out to investigate the effects of Salvia plebeia R. Br. ethanolic extract with different aspects (stem/leaf and whole plant) on differentiation and lipid accumulation in 3T3-L1 preadipocytes. The morphological changes and the degrees of lipid accumulation in 3T3-L1 cells were measured by Oil Red O staining and intra-cellular triglyceride (TG) assay. The mRNA expressions of special peroxisome proliferation activated receptor- genes (PPAR), CCAAT/ enhancer-binding protein (C/$EBP{\alpha}$), fatty acid synthase (FAS) and lipoprotein lipase (LPL) were detected by reverse transcriptase polymerase chain reaction (RT-PCR). The 50% ethanolic extracts ($100{\mu}g/mL$) of stem and leaf (SALE) and 30% ethanolic extracts (100 g/mL) of whole plant (SAE) from Salvia plebeia R. Br. were significantly attenuated lipid accumulation during adipogenesis in 3T3-L1 cells. Ethyl acetate-soluble fractions ($50{\mu}g/mL$) significantly inhibited lipid droplet accumulation in 3T3-L1 cells. In addition, SALE induced down-regulation of specific adipogenic transcriptional factors (C/$EBP{\alpha}$ and $PPAR{\gamma}$) and target genes (FAS and LPL) during adipogenesis. Salvia plebeia R. Br. may be used as a safe and efficient natural substance to manage obesity.

• The Korean Journal of Food And Nutrition
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• v.34 no.4
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• pp.398-406
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• 2021

This study investigated on the nutritional components and physiological activity of four wild vegetables namely Salvia plebeia R. Br., Angelica acutiloba, Gynura procumbens and Saururus chinensis Baill for the development of representative wild vegetables in Chungbuk. Salvia plebeia possessed the highest radical scavenging activity and beta-carotene, but exhibited the lowest α-glucosidase inhibitory activity compared to the other three vegetables. Angelica acutiloba showed high crude protein content and α-glucosidase inhibitory activity, but contained low total polyphenol content, radical scavenging ability and beta-carotene compared to the other three vegetables. Gynura procumbens showed high mineral content, beta-carotene, vitamin K1 content and α-glucosidase inhibitory activity, but showed the lowest total polyphenol content and radical scavenging ability compared to the other three vegetables. Saururus chinensis showed high crude fiber content and total polyphenol content, but contained the lowest mineral and vitamin K1 content. To conclude, it is suggested to use Salvia plebeia or Saururus chinensis as antioxidant food materials and Angelica acutiloba and Gynura procumbens as food materials and sources of α-glucosidase inhibitors. In particular, it is believed that Saururus chinensis, which possessed high content of crude fiber, is suitable for low-calorie food materials such as diet products.

• Advances in Traditional Medicine
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• v.1 no.2
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• pp.29-35
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• 2000

The effect of aqueous extract of Salvia plebeia R. Br. (Labiatae) (AESP) on immunoglobulin (lgE) antibody mediated allergic reactions in rats was investigated. AESP inhibited passive cutaneous anaphylaxis (PCA) when intravenously, intraperitoneally, and orally administered. AESP dose-dependently inhibited histamine release from rat peritoneal mast cells activated by anti-DNP IgE antibody. Moreover, AESP had an inhibitory effect on anti-DNP IgE antibody induced $TNF-{\alpha}$ production from RPMC. These results suggest that AESP inhibit the IgE-mediated allergic reaction in rats.

• Natural Product Sciences
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• v.16 no.4
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• pp.207-210
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• 2010

Phytochemical investigation of Salvia plebeia resulted in the isolation of nine compounds. Their structures were determined to be 6-methoxynaringenin (1), 6-methoxynaringenin-7-O-$\beta$-D-glucoside (2), hispidulin (3), homoplantaginin (4), nepetin (5), nepitrin (6), 6-hydroxyluteolin (7), caffeic acid (8) and rosmarinic acid (9) by spectroscopic analyses. 6-Methoxynaringenin (1), 6-hydroxyluteolin (7) and rosmarinic acid (9) were isolated from this plant for the first time.

• Korean Journal of Pharmacognosy
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• v.41 no.1
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• pp.31-37
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• 2010

The Salvia plebeia R. which is the biennial plant belonging to the Labiatae department, grows naturally in the Korea entire area. Presently, its extract (SPRE) is known to have an anti-inflammation and anti-allergy activity, but there are a few evidences about it. SPRE inhibits pro-inflammatory cytokine such as TNF-$\alpha$ and IL-6 as well as nitric oxide (NO) production in lipopolysaccharide (LPS) treated- macrophages. The co-administration of SPRE during OVA sensitization significantly reduced total IgE levels in mice. The mice who received SPRE co-administered with OVA showed a significant increase in serum OVA-specific IgG2a/b levels. Spleen-cell cultures harvested from OVA-sensitized mice showed a significant decrease in Th2 cytokine levels with a concomitant increase in Th1 cytokine levels only when SPRE co-administered with OVA. These results demonstrate that SPRE can control the LPS-induced inflammatory reaction and prevent antigen-induced Th2 immune responses in mice.

• Korean Journal of Pharmacognosy
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• v.32 no.1 s.124
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• pp.55-60
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• 2001

The comparison of $IC_{50}$ values of Salvia plebeia R. Br. extracts on L1210, $P388D_1$ cancer and Vero normal cell lines showed that the n-hexane soluble extract of S. plebeia R. Br. retains the most growth-inhibitory activity against tumor cell lines. The minimum inhibitory concentration (MIC) of the extract against microorganisms were also examined. Antimicrobial activity of amocla and ketoconazole as references was compared to those of other solvent extracts such as $H_2O$, n-hexane, chloroform, ethyl acetate and methanol. The antimicrobial activity of extract had growth inhibition activity against gramnegative bacteria, gram-positive bacteria and fungi $(MIC\;>\;200\;{\mu}g/ml)$ except for n-hexane extract. Seven bacterial strains were tested for in vitro susceptibility to the extract of S. plebeia R. Br. However the n-hexane extract of S. plebeia R. Br. inhibited the growth of several bacterial strains (MIC values between 100 and $200\;{\mu}g/ml$ for gram positive bacteria, $25\;{\mu}g/ml$ for P. putida).

• Korean Journal of Pharmacognosy
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• v.32 no.4 s.127
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• pp.297-301
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• 2001

We studied the inhibitory effect of the aqueous extract of Salvia plebeia (SPAE) on immediate hypersensitivity reactions. SPAE inhibited immediate hypersensitivity reaction induced by compound 48/80 in mice. When SPAE was employed in an immediate hypersensitivity reaction test, the plasma histamine levels were reduced in a dose-dependent manner. SPAE also inhibited the histamine release from rat peritoneal mast cells (RPMC) by compound 48/80. The level of cAMP in RPMC, when SPAE was added, significantly increased compared with that of a normal control. These results indicate that SPAE may be beneficial in the treatment of immediate hypersensitivity reactions.

• Natural Product Sciences
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• v.15 no.2
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• pp.106-109
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• 2009

Phytochemical investigations of the EtOAc-soluble fraction of the whole plants of Salvia plebeia using repeated column chromatography with preparative HPLC led to the isolation of a new phenylbutanone glucoside, 4-{4-0-[6-(4-hydroxybenzoyl)-0-${\beta}$-D-glucopyranosyl]-3-hydroxyphenyl}-butan-2-one (salviaplebeiaside, 1) along with two known phenolic compounds, rosmarinic acid methyl ester (2) and luteolin-7-0-${\beta}$-D-glucoside (3). The structures of these compounds were determined on the basis of spectroscopic methods including 1D-, 2D-NMR and MS spectrometry and comparison of spectroscopic data with those of values reported in the literatures.

• Journal of Bio-Environment Control
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• v.26 no.3
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• pp.208-214
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• 2017

This study aimed to determinate the effect of fluorescent light and light-emitting diodes on the leaf morphology, growth and antioxidant capacity of Salvia plebeia. The plants were grown for 56 days after transplanting (DAT) under the fluorescent light (FL) and LEDs (White, Red and Blue (R+B, ratio 2:1), Blue, Red LED) under the same light intensity and photoperiod ($130{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 12 hours). Leaf length, width and number of Salvia plebeia at the 56 DAT were significantly higher under the FL and red LED, and lower in the RB LED and white LED. The highest fresh and dry weights of shoot and leaf area were observed in the red LED, followed by the FL and blue LED, and the lowest in the RB LED and white LED. After 21 DAT, leaf apinasty symptom was appeared in plants grown under red LED and RB LED. The chlorophyll content was lower in the red LED. The specific leaf weight, the ratio of leaf dry weight to area, was higher in the blue LED, and lowest in the FL. No significant difference in DPPH radical scavenging activity of Salvia plebeia under the different light sources. All the integrated results suggest that the FL light is a proper light conditions for a closed cultivation of Salvia plebeia.