Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Antiadipogenic Effects of Salvia plebeia R. Br. Extracts by Extraction Conditions in 3T3-L1 Preadipocytes

추출조건에 따른 배암차즈기 추출물의 지방세포 분화 및 지방축적 억제 효과

  • Choi, Soo Im (YD Life Science Research Institutes, YD Life Science Co. Ltd.) ;
  • Kwak, Hoyoung (YD Life Science Research Institutes, YD Life Science Co. Ltd.) ;
  • Kim, Jae Yoon (YD Life Science Research Institutes, YD Life Science Co. Ltd.) ;
  • Choi, Jong Gil (YD Life Science Research Institutes, YD Life Science Co. Ltd.) ;
  • Lee, Je Hyuk (Department of Food and Nutrition, Kongju National University)
  • 최수임 ((주)와이디생명과학연구소) ;
  • 곽호영 ((주)와이디생명과학연구소) ;
  • 김재윤 ((주)와이디생명과학연구소) ;
  • 최종길 ((주)와이디생명과학연구소) ;
  • 이제혁 (공주대학교 식품영양학과)
  • Received : 2015.03.02
  • Accepted : 2015.05.04
  • Published : 2015.06.30
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Abstract

This study was carried out to investigate the effects of Salvia plebeia R. Br. ethanolic extract with different aspects (stem/leaf and whole plant) on differentiation and lipid accumulation in 3T3-L1 preadipocytes. The morphological changes and the degrees of lipid accumulation in 3T3-L1 cells were measured by Oil Red O staining and intra-cellular triglyceride (TG) assay. The mRNA expressions of special peroxisome proliferation activated receptor- genes (PPAR), CCAAT/ enhancer-binding protein (C/$EBP{\alpha}$), fatty acid synthase (FAS) and lipoprotein lipase (LPL) were detected by reverse transcriptase polymerase chain reaction (RT-PCR). The 50% ethanolic extracts ($100{\mu}g/mL$) of stem and leaf (SALE) and 30% ethanolic extracts (100 g/mL) of whole plant (SAE) from Salvia plebeia R. Br. were significantly attenuated lipid accumulation during adipogenesis in 3T3-L1 cells. Ethyl acetate-soluble fractions ($50{\mu}g/mL$) significantly inhibited lipid droplet accumulation in 3T3-L1 cells. In addition, SALE induced down-regulation of specific adipogenic transcriptional factors (C/$EBP{\alpha}$ and $PPAR{\gamma}$) and target genes (FAS and LPL) during adipogenesis. Salvia plebeia R. Br. may be used as a safe and efficient natural substance to manage obesity.

Keywords

References

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