• Asian-Australasian Journal of Animal Sciences
• /
• v.13 no.10
• /
• pp.1407-1413
• /
• 2000

Oil palm frond (OPF) is a new non-conventional fibrous feed for ruminants. Evaluation on the nutritive values and digestibility of OPF was carried out using goats. In a completely randomised design, 20 local male goats were assigned to evaluate fresh and different types of processed OPF. A 60 day feeding trial was done to determine the digestible nutrient intake of fresh, ensiled and pelleted OPF and its response on live weight gain of goat. The pelleting of OPF increased (p<0.05) intake compared to fresh or ensiled OPF. The OPF based mixed pellet (50% OPF with 15% palm kernel cake, 6% rice bran, 6% soybean hull, 15% molasses, 2% fishmeal, 4% urea, 1.5% mineral mixture and 0.5% common salt) increased (p<0.05) nutrient intake, digestibility and reduced feed refusals. The mixed pellet also increased digestible dry matter intake (DDMI) and digestible organic matter intake (DOMI) at 80% and 63% level respectively than the fresh OPF. The increased digestible nutrient intake on the OPF based mixed pellet, resulted in increased live weight gain of goats. Furthermore, OPF has a good potential as a roughage source when it is used with concentrate supplement. OPF based formulated feed in a pelleted form could be used as a complete feed for intensive production of goat and other ruminants.

• Journal of Powder Materials
• /
• v.23 no.3
• /
• pp.189-194
• /
• 2016

We report a facile method for preparing KIT-1 mesoporous silicates with two different macroporous structures by dual templating. As a template for macropores, polystyrene (PS) beads are assembled into uniform three dimensional arrays by ice templating, i.e., by growing ice crystals during the freezing process of the particle suspension. Then, the polymeric templates are directly introduced into the precursor-gel solution with cationic surfactants for templating the mesopores, which is followed by hydrothermal crystallization and calcination. Later, by burning out the PS beads and the surfactants, KIT-1 mesoporous silicates with macropores are produced in a powder form. The macroporous structures of the silicates can be controlled by changing the amount of EDTANa4 salt under the same templating conditions using the PS beads and inverse-opal or hollow structures can be obtained. This strategy to prepare mesoporous powders with controllable macrostructures is potentially useful for various applications especially those dealing with bulky molecules such as, catalysis, separation, drug carriers and environmental adsorbents.

• Journal of Integrative Natural Science
• /
• v.10 no.4
• /
• pp.175-191
• /
• 2017

In organic chemistry amide synthesis is performed through condensation of a carboxylic acid and an amine with releasing one equivalent of water via the corresponding ammonium carboxylate salt. This method is suffering from tedious processes and poor atom-economy due to the adverse thermodynamics of the equilibrium and the high activation barrier for direct coupling of a carboxylic acid and an amine. Most of the chemical approaches to amides formations have been therefore being developed, they are mainly focused on secondary amides. Direct carbonylations of tertiary amines to amides have been an exotic field unresolved, in particular direct carbonylation of trimethylamine in lack of commercial need has been attracted much interests due to the versatile product of N,N-dimethylacetamide in chemical industries and the activation of robust N-C($sp^3$) bond in tertiary amine academically. This review is focused mainly on carbonylation of trimethylamine as one of the typical tertiary amines by transition metals of cobalt, rhodium, platinum, and palladium including the role of methyl iodide as a promoter, the intermediate formation of acyl iodide, the coordination ability of trimethylamine to transition metal catalysts, and any possibility of CO insertion into the bond of Me-N in trimethylamine. In addition reactions of acyl halides as an activated form of acetic acid with amines are reviewed in brief since acyl iodide is suggested as a critical intermediate in those carbonylations of trimethylamine.

• Bulletin of the Korean Chemical Society
• /
• v.34 no.12
• /
• pp.3559-3564
• /
• 2013

Alumina particles were grafted onto the surface of manganese oxide particles via the coprecipitation process using surfactant and cosurfactant. The phase of Mn/Al salts (Phase I) and the phase of precipitation agent (Phase II) were prepared in aqueous surfactant solution, separately. Phase II was added into Phase I and the reaction was performed to form the precursors of composites through hydrogen bonding between $Mn(OH)_2$ and $Al(OH)_3$ prepared by the reaction of Mn/Al salts with the precipitation agent. The alumina-grafted manganese oxide particles were obtained as a final product after calcination. The concentrations of Al salt and surfactant were varied to investigate their effects on the formation and the crystallinity of composites. In addition, the crystal structure of products could be controlled by changing the calcination temperature. Through thermal analyses, it was found that the thermal stability of manganese oxide was improved by the introduction of alumina on its surface.

• BMB Reports
• /
• v.45 no.12
• /
• pp.748-753
• /
• 2012

A sequence of 10 amino acids at the C-terminus region of methylglyoxal synthase from Escherichia coli (EMGS) provides an arginine, which plays a crucial role in forming a salt bridge with a proximal aspartate residue in the neighboring subunit, consequently transferring the allosteric signal between subunits. In order to verify the role of arginine, the gene encoding MGS from a thermophile species, Thermus sp. GH5 (TMGS) lacking this arginine was cloned with an additional 30 bp sequence at the 3'-end and then expressed in form of a fusion TMGS with a 10 residual segment at the C-terminus ($TMGS^+$). The resulting recombinant enzyme showed a significant increase in cooperativity towards phosphate, reflected by a change in the Hill coefficient (nH) from 1.5 to 1.99. Experiments including site directed mutagenesis for Asp-10 in TMGS and $TMGS^+$, two dimentional structural survey, fluorescence and irreversible thermoinactivation were carried out to confirm this pathway.

• Journal of Genetic Medicine
• /
• v.10 no.2
• /
• pp.81-87
• /
• 2013

Pseudohypoaldosteronism (PHA), a rare syndrome of systemic or renal mineralocorticoid resistance, is clinically characterized by hyperkalemia, metabolic acidosis, and elevated plasma aldosterone levels with either renal salt wasting or hypertension. PHA is a heterogeneous disorder both clinically and genetically and can be divided into three subgroups; PHA type 1 (PHA1), type 2 (PHA2) and type 3 (PHA3). PHA1 and PHA2 are genetic disorders, and PHA3 is a secondary disease of transient mineralocorticoid resistance mostly associated with urinary tract infections and obstructive uropathies. PHA1 includes two different forms with different severity of the disease and phenotype: a systemic type of disease with autosomal recessive inheritance (caused by mutations of the amiloride-sensitive epithelial sodium channel, ENaC) and a renal form with autosomal dominant inheritance (caused by mutations of the mineralocorticoid receptor, MR). In the kidneys, the distal nephron takes charge of the fine regulation of water absorption and ion handling under the control of aldosterone. Two major intracellular actors necessary for the action of aldosterone are the MR and the ENaC. Impairment of the intracellular aldosterone signal transduction pathway results in resistance to the action of mineralocorticoids, which leads to PHA. Herein, ion handling the distal nephron and the clinico-genetic findings of PHA are reviewed with special emphasis on PHA type 1.

• Bulletin of the Korean Chemical Society
• /
• v.31 no.9
• /
• pp.2627-2632
• /
• 2010

Hormone sensitive lipase (HSL) plays a major role in energy homeostasis and lipid metabolism. Several crystal structures of HSL-homolog proteins have been identified, which has led to a better understanding of its molecular function. HSL-homolog proteins exit as both monomer and dimer, but the biochemical and structural basis for such oligomeric states has not been successfully elucidated. Therefore, we determined the crystal structure of HSL-homolog protein EstE7 from a metagenome library at $2.2\;{\AA}$ resolution and characterized the oligomeric states of EstE7 both structurally and biochemically. EstE7 protein prefers the dimeric state in solution, which is supported by its higher enzymatic activity in the dimeric state. In the crystal form, EstE7 protein shows two-types of dimeric interface. Specifically, dimerization via the external ${beta}8$-strand occurred through tight association between two pseudosymmetric folds via salt bridges, hydrogen bonds and van der Waals interactions. This dimer formation was similar to that of other HSL-homolog protein structures such as AFEST, BEFA, and EstE1. We anticipate that our results will provide insight into the oligomeric state of HSL-homolog proteins.

• The Korean Journal of Ecology
• /
• v.6 no.3
• /
• pp.177-186
• /
• 1983

Vegetation types and their standing crop in the sand dune on the south coast of Korea was investigated by the method of Curtis, J.T. and McIntosh, R.P. (1951). The relationship between vegetation types and environmental factors was also analyzed. The dominant species in the vegetations of the south coast sand dune were Carex pumila, Calystegia soldamella, Imperata cylindrica var. koenigii, Vitex rotundifolia, Ixeris repens, Carex kobomugi, Zoysia macrostachya. The species density in the sand dune vegetation increased with the distance from the coast, psammophyte and rhizome psammophyte decreased with the distance from the coast but other plants increased. The standing crop of the sand dune vegetatiion was average $53.79g/m^2$. An individual standing crop of Vitex routundifolia and Carex kobomugi varied with the curve of secondary degree. The salt content of the sand dune soil from 2.95 to 11.78 mg %, and it was not significant differences among stands, but it was varied with the distance form the coast. Negative relationship between warmth index and aboveground standing crop was found and the formula y=283.8886 - 2.4910X could be estimated.

• Transactions of the Korean Society of Mechanical Engineers
• /
• v.19 no.7
• /
• pp.1731-1740
• /
• 1995

Experimental investigations have been made to study the double-diffusive nature of convection of an initially stratified salt-water solution due to heating from below in a rotating cylindrical cavity. The objective is to examine the flow phenomena and the heat transfer characteristics according to the changes in temperature gradient, concentration gradient and rotating velocity of cavity. Thermal and solutal boundary conditions at side wall are adiabatic and impermeable, respectively. The top and bottom plate are maintained each at constant temperature and concentration. The cavity is put into a state of solid body rotation. Like the stationary case, the types of initially-formed flow pattern are classified into three regimes depending on the effective Rayleigh number and Taylor number; stagnant flow regime, single mixed-layer flow regime and successively formed multi-mixed layer flow regime. At the same effective Rayleigh number, the number of initially-formed mixed layer and its growth rate decrease as the effect of rotation increases. The temperature and concentration profiles are both uniform in each layer due to convective mixing in the layered-flow regime, but look both liner in stagnant flow regime and single mixed-layer flow regime. At the interface between adjacent layers, the temperature changes smoothly but the concentration changes rapidly.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.26 no.3
• /
• pp.376-382
• /
• 1997

Antioxidative characteristics of doenjang(fermented soybeans paste) phenolics on the lipid oxidation systems were studied by the determination of the oxidative related activity including lipoxygenase (LOX) inhibition, metal chelating and free radical scavengning of doenjang phenolics. Manlikong variety containing the highest amounts of phenolic compounds among the soybean variety, was used for doenjang processing. Doenjang was prepared by the series of processes including soaking for overnight, cooking for 1hr at 12Lb, first fermentation (3 days at 30$\pm$2$^{\circ}C$) for the preparation of meju(soybean koji) after inoculation of Asp. oryzae, and further fermentation(60 days at 30$\pm$2$^{\circ}C$) for the ripening after addition of salt 13% to meju. In order to investigate the antioxidative activity of phenolics in doenjang, the doenjang phenolics was extracted with methanol form freeze dried defatted doenjang. Antioxidative effects of methanol extract on linoleic acid oxidation system were observed by the significantly decreased levels of peroxide and conjugated diene formation. In addition, methanol extract resulted in the inhibition of LOX activity. and also, metal(FeCl$_3$) chelation and free radical scavengning activities were increased with increasing concentration of methanol extract.