• 대한한의학회지
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• 제29권3호
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• pp.1-10
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• 2008

Objectives: This study was to assess the toxicity of Sipjeondaebo-tang by bacterial reverse mutation test. Methods: In this study, to evaluate the bacterial reverse mutation of Sipjeondaebo-tang water-extract, the in vitro Ames test using Salmonella typhimurium (TA98, TA100, TA1,535, TA1,537) and Escherichia coli(WP2uvrA) were performed with Sipjeondaebo-tang water extract at the concentrations 0, 312, 625, 1250, 2500 and 5000 ${\mu}g/plate$. Results: Sipjeondaebo-tang water extract was negative in Ames test with both Salmonella typhimurium or Escherichia coli with and without rat liver microsomal enzyme (S9- fraction and S+ fraction). Conclusions: According to these results, we concluded that a Sipjeondaebo-tang water extract did not cause bacterial reverse mutation.

• 한국식품위생안전성학회지
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• 제20권3호
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• pp.175-178
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• 2005

Clean Watural은 주성분이 참나무로부터 추출정제한 목초액과 프로폴리스로 구성된 새로운 천연 살균소독제이다. Salmonella typhimurium TA98, TA100, TA102, TA1535 및 TA1537 등의 5종의 균주를 이용한 복귀돌연변이성 시험을 수행한 결과, 모든 균주에서 대조군과 비교하여 시험용량군 사이에 돌연변이 유발성이 관찰되지 않았다. 그러나, 앙성대조물질에서는 대조군과 비교하여 통계학적으로 유의한 균주의 증가가 관찰되었다. 따라서, 시험물질인 천연 살균소독제, Clean Natulat은 돌연변이 유발 가능성이 없는 것으로 평가되었다.

• Toxicological Research
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• 제4권2호
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• pp.131-142
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• 1988

The post-mitochondrial liver fractions (S-9) were prepared from rats and hamsters which have been treated with Aroclor 1254 (PCB) and the capacities of these S-9 fractions to generate mutagenic metabolites from several well known procarcinogens have been compared. Benzo(a)pyrene (B(a)P), 3-methylcholanthrene (3-MC), Aflatoxin B1(AFB1), 2-acetylamino-fluorene(AAF), and 2-aminofluorene (AF) were employed as promutagens in the Salmonella reverse mutation tests. Results showed that the rat and hamster S-9 fractions had differential abilities to produce mutagenic metabolites from a given promutagen.

• 한국식품위생안전성학회지
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• 제19권4호
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• pp.193-197
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• 2004

산삼배양추출물의 세균에서의 돌연변이 유발성 검색을 위하여 Salmonella typhimurium의 히스티딘 요구성 균주 LA100, TA1535, TA98 및 TA1537의 4개의 균주와 대장균 Escherichia coli의 트립토판 요구성 균주인 WP2 uvrA를 이용해 복귀돌연변이 시험을 실시하였다 시험물질은 멸균생리식염수에 용해하여 처리하였다. 대사활성계 적용 및 미적용시 모든 균주에 대해 0, 62, 185, 556, 1,667 및 5,000{\mu}g/plate의 범위를 설정하고 각각 음성 및 양성대 조군으로 시험군을 구성해 본 시험을 실시하였다. 시험 결과 모든 균주에서 최고농도에 이르기까지 집락수의 일관성 있는 증가는 나타나지 않았다. 이상의 결과를 종합할 때, 시험물질 산삼배양추출물은 본 시험조건 하에 사용한 시험균주들의 복귀돌연변이를 유발하지 않는 것으로 사료된다.

• 한국식품위생안전성학회지
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• 제14권3호
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• pp.259-264
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• 1999

Xylooligosaccharide의 세균에 대한 돌연변이 유발성을 검색하기 위하여 Salmonella typhimurium의 히스티딘 요구성균주 TA100, TA1535, TA98 및 TA1537의 4개 균주와 대장균 Escherichia coli의 트립토판 요구성 균주인 WP2 uvrA를 이용해 복귀돌연변이 시험을 실시하였다. 시험 물질은 증류수에 용해하여 처리하였으며, 대사 활성계 미적용 및 적용하에 $5000\;\mu\textrm{g}/plate$를 최고농도로 하고 공비 2로서 5단계 농도군(313, 625, 1250, 2500 및 $5000\;\mu\textrm{g}/plate$)과 음성 및 양성 대조군으로 시험군을 구성해 본 시험을 실시하였다. 시험 결과 시험물질을 처리한 모든 농도군에서 복귀돌연변이 집락 수는 음성대조군과 비슷한 정도로 관찰되었다. 이상의 결과에서, xylooligosaccharide는 본 시험 조건 하에 사용한 균주들에 복귀돌연변이를 유발하지 않는 것으로 사료되었다.

• Environmental Analysis Health and Toxicology
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• 제21권4호
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• pp.317-322
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• 2006

We have investigated the genotoxicity of 1,2,4-trimethylbenzene using Ames reverse mutation test. In Ames reverse mutation test, 1,2,4-trimethylbenzene treatment at the dose of 100, 50, 25, 12.5, $6.25{\mu}g/plate$ did not induce mutagenicity in Salmonella typhimurium TA98, TA100, TA1535, TA1537 and in Escherichia coli WP2uvrA with and without metabolic activation. These results indicate that 1,2,4-trimethylbenzene has no mutagenic potential under the rendition in this study.

• 대한약침학회지
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• 제27권2호
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• pp.154-161
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• 2024

Objectives: The objective of this study was to assess the genotoxicity of a no-pain pharmacopuncture (NPP) extract developed in 2022 using a bacterial reverse mutation assay, aiming to further substantiate the safety profile of NPP. Methods: The genotoxicity evaluation involved a bacterial reverse mutation assay to assess the mutagenic potential of NPP extracts with and without metabolic activation. Histidine-requiring Salmonella typhimurium strains (TA98, TA100, TA1535, and TA1537) and tryptophan-requiring Escherichia coli strains (WP2uvrA) were used in the assay. Results: The NPP extract did not induce a revertant colony count exceeding two times that of the negative control at any dose level in any of the tested strains, both with and without metabolic activation. Additionally, no growth inhibition or precipitation was observed in the presence of NPP. Conclusion: Based on the findings, it can be concluded that the NPP extract exhibited no mutagenic potential in the in vitro genotoxicity tests conducted.

• 한국환경성돌연변이발암원학회지
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• 제18권2호
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• pp.129-134
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• 1998

To evaluate the genotoxicity of CKD-602, an anticancer agent the in viかo reverse mutation assay using Salmonella typhimurium, the Chromosome aberration assay using Chinese hamster lung (CHL) cells and the in vivo micronucleus assay using bone marrow cells of ddY mice were performed. In the reverse mutation assay, CKD-602 did not induced mutagenicity in Salmonella typhimurium TA 98, TA 100, TA 1535, and TA 1537 strains with and without metabolic activation. In the chromosome aberration test using CHL cells, there was an increased incidence of structural aberrations induced by CKD-602 without metabolic activation during 24 and 48 hours, but CKD-602 did not induce chromosome aberration with metabolic activation. The in vivo induction of micronuclei was measured in polychromatic erythrocytes of bone marrow of male ddY mice. At 24 hours after treatment with CED-602 by i.p. once, there was an increased incidence of micronucleated polychromatic erythrocytes in bone marrow of ddY male mice.

• 한국환경성돌연변이발암원학회지
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• 제18권2호
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• pp.123-128
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• 1998

To evaluate the genotoxicity of SKP-450, an antihypertensive agent the in vitro reverse mutation assay using Salmonella typhimurium, the Chromosome aberration assay using Chinese hamster lung (CHL) cells and the in vivo micronucleus assay using bone marrow cells of ddY mice were performed. In the Reverse mutation test, SKP-450 did not induced mutagenicity in Salmonella typhimurium TA 98, TA 100, TA 1535 and TA 1537 with and without metabolic activation. In the chromosome aberration assay using CHL cells, there was no increased incidence of structural and numerical aberrations with and without metabolic activation. The in vivo induction of micronuclei was measured in polychromatic erythrocytes of bone marrow of male ddY mice at 30 hours after treatment with SKP-450 by p.o once. The results showed no increased incidence of micronucleated polychromatic erythrocytes in bone marrow of ddY male mice treated with SKP-450.

• Biomolecules & Therapeutics
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• 제8권3호
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• pp.248-254
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• 2000

(R)-JG-381, a R form of alkylglycidic acid derivative, was examined for mutagenicity in the reverse mutation test on bacteria, chromosomal aberration test on cultured mammalian cells and micronucleus test in mice. In the reverse mutation test on bacteria using Salmonella typhimurium strain TA98, TA100, TA102, TA1535, TA1537 with or without a metabolic activation system (S9 mix), (R)-JG-381 did not affect the revertant colonies but significantly increased revertant colonies in one test strain, TA98, compared with the vehicle control. In the chromosomal aberration (CA) test using cultured Chinese Hamster Lung fibroblast(CHL) cells, the number of aberrant cells was clot increased in the presence or absence of 59 mix at concentration of the (R)-JG-381 0.025 $\mu$l/m1 to 0.1 $\mu$l/m1, compared with vehicle control. In the micronucleus (MN) test, micronucleated polychromatic erythrocytes in the (R)-JG-381-treated mice were not different from those of the vehicle-treated mice.