Toxicological Research
- Volume 4 Issue 2
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- Pages.131-142
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- 1988
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- 1976-8257(pISSN)
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- 2234-2753(eISSN)
USE OF A MIXED METABOLIC ACTIVATION SYSTEM IN THE SALMONELLA REVERSE MUTATION TEST OF CHEMICAL CARCINOGENS
- Oh, Goo-Taeg (Genetic engineering Center Korea Advanced Institute of Science and Technology) ;
- Kim, Won-Yong (Genetic engineering Center Korea Advanced Institute of Science and Technology) ;
- Park, Jae-Youn (Genetic engineering Center Korea Advanced Institute of Science and Technology) ;
- Lee, Chang-Eop (College of Pharmacy, Chungnam National University) ;
- Kim, Hwan-Mood (Genetic engineering Center Korea Advanced Institute of Science and Technology)
- Published : 1988.12.01
Abstract
The post-mitochondrial liver fractions (S-9) were prepared from rats and hamsters which have been treated with Aroclor 1254 (PCB) and the capacities of these S-9 fractions to generate mutagenic metabolites from several well known procarcinogens have been compared. Benzo(a)pyrene (B(a)P), 3-methylcholanthrene (3-MC), Aflatoxin B1(AFB1), 2-acetylamino-fluorene(AAF), and 2-aminofluorene (AF) were employed as promutagens in the Salmonella reverse mutation tests. Results showed that the rat and hamster S-9 fractions had differential abilities to produce mutagenic metabolites from a given promutagen.