• Journal of Environmental Science International
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• v.16 no.2
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• pp.241-245
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• 2007

We studied concentration of heavy metals in Sagunjatang. In this experiment was analyzed the concentration of heavy metals of boiled Sagunjatang, decoction and its ingredient herbal medicines. The concentration of heavy metals(As, Pb, and Cd) were analysed using ICP-AES, and Hg was analysed by mercury analyzer. The average concentration of heavy metals in Sagunjatang were as follows : In all ingredient herbal medicines (Glycyrrhizae Radix, Atractylodes Macrocephala, Poria Cocos and Jinseng) of Sagunjatang, As(arsenite) contents in ail samples was in the range of 0.369-0.723ppm, Cd(cadmium) was in the range of 0.000-0.085ppm, Pb(lead) was in the range of 0.059-0.871ppm and Hg(mercury) was in the range of 0.001-0.004ppm. In boiled Sagunjatang, the concentration of heavy metals(As, Pb, Cd and Hg) was in the range of 0.000-0.016ppm, respectively. In the decoction of herbal medicines after boiled, the concentration of heavy metals was in the range of 0.004-0.387ppm. These results suggest that Sagunjatang which we take is less harmful than herbal medicine itself, and there are more significant for using the decoction of herbal medicines.

• Journal of Haehwa Medicine
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• v.20 no.1
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• pp.175-182
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• 2011

This study aims to summarize and make a reference of Sagunjatang and Samultang by investigating and comparing the effects of prescriptions. In this process, this review surveyed all papers used Sagunjatang and Samultang in PubMed database and finally 36 papers were included. The publication year, the nations of authors, the type of materials, the formation of experiments, the type of herbal medicine, and their action and mechanisms were analyzed. The number of studies on Sagjunjatang and Samultang has increased since 1990's. The main formation of experiments was in vivo study and the portion was 55%. The proportion of the research using prescriptions was 71%. Sagunjatang was investigated in the field of GI track function, anticancer effect, radioprotective effect and etc. Samultang was studied in the field of anticancer effect, brain cognitive function, anti-inflammatory effect, cell protective effect, and etc. Based on these results, Sagunjatang and Samultang showed the different bioactivities. This study will provide the useful information on differentiation and analysis of the effect of herbal prescriptions by the theory of Korean traditional medicine.

• The Journal of Internal Korean Medicine
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• v.23 no.2
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• pp.202-211
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• 2002

1. Background The previous studies on anticancer medicine derived from korean traditional medicine have focused on the life elongation of cancer cell bearing animals. However, it is thought that more molecular biological studies are needed to reveal their mechanism. 2. Objective The aim of this study was to investigate the molecular biological function of Sagunjatang plus Cremastrae Appenediculatae Tuber on cytostaticity, apoptosis and apoptosis related genes revelation against human stomach cancer cells(AGS). 3. Methods After administrating Sagunjatang and Sagunjatang plus Cremastrae Appenediculatae Tuber to human stomach cancer cell. MTT assay was performed to compare and examine the efficacy of each medicine on the cytostaticity of stomach cancer cells in proportion to time and doses, and apoptosis assay was performed to examine their effect on apoptosis by using DAPI dye and counting the number of cells which developed in an apoptotic body. In addition, the quantitative RT-PCR was used to examine their effect on the revelation of Bcl-2, Bax and P53, which are genes related to apoptosis. 4. Result and Conclusion Sagunjatang plus Cremastrae Appenediculatae Tuber demonstrated increased cytostaticity. decreased apoptosis and unremarkable revelation of apoptosis related genes. But in the cytostaticity and apoptosis, Sagunjatang plus Cremastrae Appenediculatae Tuber showed a tendency to control stomach cancer cells. Therefore, we can expect the clinical application to the related diseases. Besides, it needs another experiment on various cancer cells, such as, lung cancer cell and hysterocarcinoma cell.

• The Journal of Pediatrics of Korean Medicine
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• v.13 no.1
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• pp.253-275
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• 1999

The effects of Sagunjatang and Samultang on the immunosuppression induced by methotrexate(MTX) in rats were investigated in this study. The multiple parameters of immunity assessed in each rats included leukocyte count, lymphocyte rate, the number of lymphocyte in tibial bone marrow, contact hypersensitivity to DNFB, morphological change of thymocyte and IgG antibody on SDS-PAGE. Sprague-Dawley male rats were used and divided into five groups at random. Group A was normal control. Group B, the MTX treatment control, was injected i.v. with 2mg/kg of on days 9, 11 after sensitization with SRBC on 5th day. Group C, the experimental control, was treated Sagunjatang for 18days and MTX. Group D was treated Samultang for 18days and MTX. Group E was treated Sagunjatang-Samultang for 18days and MTX. The dosage of Sagunjatang and Samultang was $1m{\ell}/day$ respectively. In the case of Group E, rats Were fed Sagunjatang $1m{\ell}$ in the morning and Samultang $1m{\ell}$ in the afternoon. The results are summarized as follows: 1. Leukocyte count in rats induced by intravenous sensitization with SRBC was decreased significantly in Group E. 2. Leukocyte counts of 2weeks later after being treated MTX were increased significantly in Groups C and D. 3. Lymphocyte rate in rats induced by intravenous sensitization with SRBC wasn't changed significantly in all the experimental groups. 4. Lymphocyte rate of 2weeks. later after being treated MTX was increased significantly in Group D. 5. The number of lymphocyte in tibial bone marrow was incereased significantly in Group C. 6. Contact hypersensitivity wasn't changed significantly in all the experimental groups. 7. Morphological finding of thymocyte in group C was similar to normal group as compared with control group. 8. Purified IgG of all the experimental groups showed two bands of 50,000 and 25,000 on SDS-PAGE. But there was no difference among experimental groups.

• Journal of Korean Medical classics
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• v.19 no.4
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• pp.219-225
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• 2006

The result is the followings after investigating composition, dosage and usage of Sagunjatang(四君子湯), Samultang(四物湯), Palmultang(八物湯), Sipjeondaebotang(十全大補湯) in literature by country and Era 1. Sagunjatang has become equal to Panax ginseng(人蔘), Atractylodes japonica(白朮), Poria cocos(복령) each for 2 Jeon(錢), Glycyrrhiza uralensis(甘草) 1 jeon since the Ming Dynasty in China and it is standardized by Panax ginseng, Atractylodes japonica, Poria cocos and Glycyrrhiza uralensis each for 1 Jeon 2 Pun(分) 5 Ri(里) since Dongeuibogam in Korea. 2. Samultang has become equal to Angelica gigas(當歸) and Rehmannia glutinosa(地黃) each three Jeon, Cnidium officinale(川芎) is 1 Jeon 2 Pun or 1 jeon and Paeonia lactiflora(芎藥) 2 Jeon since the Ming Dynasty in China and it is standardized by Angelica gigas, Cnidium officinale, Paeonia lactiflora and Rehmannia glutinosa each for 1 Jeon 2 Pun 5 Ri since Dongeuibogam in Korea. 3. In the case of Palmultang, compositional usage of medicine was different in the Ming Dynastry and the Ching(靑) Dynastry in China. Total dosage was increased and ratio of Angelica gigas and Rehmannia glutinosa was increased comparatively in the Ching Dynasty. All prescription of medicine was consisted of 1 Jeon 2 Pun and specific dosage was presented except Euirimchwalyo(醫林撮要) that dosage was not recorded in Korea. 4. Sipjeondaebotang tended to increase total dosage in the latter part in China. Dosage was mostly used 1 Jeon thus Dongeuibogam but Bangyakhap(方藥合編) used 1 Jeon 5 Pun differentially.

• Herbal Formula Science
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• v.15 no.2
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• pp.1-8
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• 2007

The source of Gwibitang(歸脾湯) is the book of Jesaebang(濟生方), The formulae is excluded Angelicae gigantis radix(當歸) and Polygalae radis(遠志) in the Jesaebang(濟生方), Jn the Guch iryuyo(口齒類要), Jeongcheryuyo(正體類要) and Gyojubuinyangbang(校註婦人良方), has been bec ame White Pria cocos Wolf(白茯笭) instead of Hoelen cum Pini Radix(白茯神), and is followed constitution of Uihagypmun(醫學入門) in the Donguibogam(東醫寶鑑). Basic formulae of Gwibitang(歸脾湯) consist of Sanjointang(酸棗仁湯), Sagunjatang(四君子湯). Hwanggitang, Danggwibohyeoltang(當歸補血湯), a kind of Jeongjihwan(定志丸類), a kind of Chongmyeongtang(聰明湯類). To deficiency of the heart and spleen, apply Sanjointang(酸棗 仁場), a kind of Jeongjihwan(定志丸), Sagunjatang(四君子湯) and Hwanggitang. To insufficiency of the spleen-gi(脾氣), apply Sagunjatang(四君子湯), Hwanggitang and a kind of Jeonssiigongsan(定志小丸). To deficiency of blood, apply Danggwibohyeoltang(當歸補血湯). Gwibitang(歸脾湯) is used for a mnesia and severe palpitation as a result of deficiency of the heart and spleen and deficiency of both gi(氣) and blood(血).

• The Journal of Korean Medicine
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• v.29 no.5
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• pp.1-13
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• 2008

Objective : This study is investigate the effects of Sagunjatang-Ga-Nokyong(SGJ-NY) treatment on regenerative responses of corticospinal tract(CST) axons in the injured spinal cord. Methods :Using rats, we damaged their spinal cord, and then applied SGJ-NY extract to the lesion. Then we observed GAP-43 and NGF protein, astrcyte, axonal regeneration responses and axonal elongation. Result :Determination of GAP-43 and NGF protein levels were increased. And increased proliferation of astrocyte and enhanced processes in astrocytes were observed by SGJ-NY treatment. Higher number of astrocytes within the injury cavity in SGJ-NY treated group were showed, yet CSPG proteins were a weaker staining in the cavity in SGJ-NY. CST axons extended into the cavity and to the caudal area in SGJ-NY treated group were increased. Conclusion : SGJ-NY treatment might increase neural activity in the injured spinal cord tissue, and improved axonal regeneration responses. In this process, activation of astrocytes may play a role in promoting enhanced axonal elongation. the current study show that SGJ-NY exerts positive activity on inducing nerve regeneration responses by elevating neural tissue migration activities.

• The Journal of Korean Obstetrics and Gynecology
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• v.25 no.4
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• pp.12-26
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• 2012

Objectives: The purpose of this study is to examine the effects of Sagunjatang-Gami(SGJT) on uterine and ovarian function in the ovariectomized rat postmenopause model. Methods: SGJT was administered in ovariectomized Wister albino female rats for three month. After that, uterine weight, uterine index, serum estradiol-$17{\beta}$ levels and phosphorylation of ERK or AKT, and histological analysis of uterus were measured to assess the impact on uterine and ovarian function in ovariectomized rats. In addition, phosphorylation of $ER{\alpha}$, ERK, AKT by SGJT in MDA-MB-231 cells were measured. To identify safety of SGJT, the cell cytoxicity in MDA-MB-231 cells and serum GOT, GPT levels were measured in ovariectomized rats. Results: The results were as follows. 1. SGJT decreased the viability of MDA-MB-231 cells in a dose-dependent manner. 2. The level of serum GOT, GPT in SGJT-treated group showed significant decrease in comparison with control group. 3. Phosphorylation of $ER{\alpha}$, ERK, AKT by SGJT in MDA-MB-231 cells were increased. 4. Uterus index in SGJT-treated group showed significant increase in comparison with control group. The level of serum estradiol-$17{\beta}$ in SGJT-treated group showed significant increase in comparison with control group. Phosphorylation of ERK or AKT by SGJT in the uterus of ovariectomized rats was increased significantly. 5. Uterus index and the level of serum estradiol-$17{\beta}$ in SGJT-treated group increased at higher rates in comparison with estrogen-treated group. Conclusions: Taken together, we suggest that SGJT has been shown to be effective in preventing postmenopausal uterine and ovarian degeneration and curing postmenopausal low estrogen related symptoms.

• Journal of Haehwa Medicine
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• v.9 no.1
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• pp.305-317
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• 2000

To prove the effect of Sagunjatang(SGJT) and Samultang(SMT) extract on the wave of Human Body and Active Oxygen experimentally, QRS & Free Radical of extract and precipitate of SGJT and SMT was measured. The results were summarized as follows ; 1. SGJT extract and precipitate, as a result of measuring QRS and Free Radical, had a considerable effect on the function of immunity & pituitary gland. 2. BaekBokRung of SGJT, as a result of measuring QRS and Free Radical, had a considerable effect on the function of heart & spleen. 3. BaekChul and Gamcho of SGJT, as a result of measuring QRS and Free Radical, had a considerable effect on the function of stomach & duodenum. 4. YinSam of SGJT, as a result of measuring QRS and Free Radical, had a considerable effect on the function of immunity & spleen. 5. SMT extract and precipitate, as a result of measuring QRS and Free Radical, had a considerable effect on the function of immunity & uterus. 6. SukJiHwang of SMT, as a result of measuring QRS and Free Radical, had a considerable effect on the function of immunity & liver. 7. DangGui of SMT, as a result of measuring QRS and Free Radical, had a considerable effect on the function of heart & spleen. 8. ChunGung of SMT, as a result of measuring QRS and Free Radical, had a considerable effect on the function of spleen & pituitary gland. 9. BaekJakYak of SMT, as a result of measuring QRS and Free Radical, had a considerable effect on the function of stomach & duodenum. 10. SMT extract had an effect on the function of female Urogenital system, SGJT extract had an effect on the function of male Urogenital system. These results suggested that SGJT and SMT extract might be usefully applied for suppresing of Active Oxygen formation, preventing the aging, curing all the disease resulted from Active Oxygen.

• Herbal Formula Science
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• v.27 no.2
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• pp.167-178
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• 2019

Objectives : This report describes 45 studies related to the use of Rhizoma dioscoreae main blended prescription from Donguibogam. Methods : The following conclusion were reached through investigations on the prescriptions that use Rhizoma dioscoreae as a key ingredient. Prescriptions that Rhizoma dioscoreae was taken as a monarch drug are utilized for 18 therapeutic purposes, for example, internal injury, stool disease, pediatric disease. In paticular, 24% of prescriptions appear in the chapter of internal injury, and 18% of those appear in the chapter of stool. Results : Prescriptions that utilize Rhizoma dioscoreae as the main ingredient are used in the treatment of indigestion, diarrhea and paralysis. Rhizoma dioscoreae is used in pathogenic factors such as water, water with damp, and used in pathology related to stomach system. Conclusions : The dosage of Rhizoma dioscoreae is 8li(about 0.29 gram) to 3jeon(about 11.25 gram), however 1jeon(about 3.75 gram) has been taken the most for clinical application. Sagunjatang and Siljangsan is the most useful base prescription which use the Rhizoma dioscoreae as the main ingredient.