• Title/Summary/Keyword: Saccharomyces

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Studies on the Yeasts for the Brewing of Soy Sauce(2) -Isolation, identification and classification of the yeasts in the soy sauce mash- (간장발효에 관여하는 효모에 관한 연구 (제2보) -간장 덧 중에 생육하는 효모에 대하여-)

  • Lee, Taik-Soo;Lee, Suk-Kun;Shin, Bo-Kyu
    • Applied Biological Chemistry
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    • v.13 no.2
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    • pp.171-180
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    • 1970
  • The yeasts in the soy sauce mash were isolated and identified, and they were classified by coloring with the treatment of TTC(2, 3, 5, triphenyltetrazolium chloride) agar and counted in process of time. The results obtained were as follows: a) The number of ordinary and osmophilic yeasts in 1 ml. of the soy sauce mash showed a tendency to be increased from the mashing to the mature stages and to decrease in the aging stages: $127{\times}10^3$ immediately after mashing, $83{\times}10^3$ 1 month after, $356{\times}10^3$ 3 months after, $1250{\times}10^3$ 6 months after and $65{\times}10^3$ 2 years after mashing in the case of ordinary yeasts, and 0 after mashing, $40{\times}10^3$ 1 month after, $81{\times}10^3$ 3 months after, $358{\times}10^3$ 6 month after and $23{\times}10^3$ 2 years after mashing in the case of osmophilic yeasts. b) 50 strains of yeasts were isolated from the soy sauce mash optionally in process of fermentation period, and they were identified as 7 genera and 18 species: 10 strains of Saccharomyces rouxii, 1 strain of Saccharomyces marxianus, 3 strains of Saccharomyces rosei, 1 strain of Saccharomyces fermentati, 6 strains of Saccharomyces mellis, 1 strain of Saccharomyces acidifaciens, 1 strain of Saccharomyces pastori, 3 strains of Pichia polymorpha, 2 strains of Hansenula anomala, 1 strain of Hansenula saturnus, 2 strains of Hansenula suaveolens, 5 strains of Nadsonia fulvescens, 8 strains of Debaryomyces hasenii, 1 strain of Debaryomyces nicotianae, 1 strain of Debaryomyces kloeckeri, 2 strains of Torulopsis sake, 1 strain of Torulopsis holmii and 1 strain of Candida pelliculasa. c) Distribution of yeasts according to the fermentation period was as follows: i) Saccharomyces rouxii, Saccharomyces marxianus, Saccharoymces rosei, Pichia polymorpha, Debaryomyces hansenii, Torulopsis sake, Candida pelliculosa, Debaryomyces nicotianae, Nadsonia fulvescens, Hansenula suaveolens and Hansenula saturnus were found in the early stages of fermentation. ii) Saccharomyces rouxii, Saccharomyces rosei, Saccharomyces fermentati, Saccharomyces mellis, Saccharomyces pastori, Hansenula anomala, Saccharomyces acidifaciens and Debaryomyces hansenii appeared in the mature stages. iii) Saccharomyces rouxii, Saccharomyces mellis, Nadsonia fulvescenes, Dedaryomyces hansenii, Debaryomyces kloeckeri, Torulopsis sake and Torulopsis holmii were distributed in the aging stages. d) TTC white yeasts were found in abundance in the early stages of fermentation and TTC red yeasts appeared more than 50 per cent in the mature and aging stages. e) The yeasts belonging to Saccharomyces mellis and Saccharomyces pastori were classified as TTC red yeasts, Saccharomyces acidifaciens were reel pink, Hansenula saturnus Debaryomyces kloeckeri, and Torulopsis holmii were pink, Saccharomyces marxianus and Nadsonia fulvescens were white and the others were the same as the description in the previous report. Saccharomyces rouxii ware classified for the most part as TTC red yeasts, and while some of them were red pink. f) Species of yeasts in the soy sauce mash were similar to those in the soy sauce koji, but the latter were not osmophilic and in the former case, the osmophilic yeasts were increased in process of fermentation period.

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김치로부터 분리한 효모가 생산하는 휘발성 화합물이 김치의 풍미에 미치는 효과

  • 김혜자;양차범;강상모
    • Microbiology and Biotechnology Letters
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    • v.24 no.4
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    • pp.512-518
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    • 1996
  • Eleven strains had been previously isolated from kimchi and identified in our laboratory. The ability of each strain in aroma production was investigated by sensory evaluation. Among them Saccharomyc s sp. YK-17, Saccharomyces sp. YK-18, Saccharomyces sp. YH-3 and Saccharomyces fermentati YK-19 produced fruity flavour. Especially, Saccharomyces fermentati YK-19 produced apple and pineapple-like flavours. Pichia media YK-11, Saccharomyces sp. YK-20 and Pichia chambardii YH-4 produced wine-like flavour. Debaryomyces sp. YK-6, Debarymyces coudertii YK-10, Saccharomyces sp. YK-12 and Pichia haplophilia YH-5 produced alcoholic flavours. Using the good flavour producing strains as starters, various groups of kimchi were fermented, and the sensory characteristics of each group such as odor, taste and total acceptability were evaluated. The acidic odor, moldy odor and taste were reduced by adding starter, while the fresh sourness odor and taste similar to fruity fiavour were increased by starter. Comparing with the control group, these odor, taste and total acceptability were increased in the starter-added groups, such as Pichia edia YK-11, Saccharomyces sp. YK-17, and Saccharomyces,fermentati YK- 19. Saccharomyces fermentati YK-19 added kimchi group was higher siginificantly (P<0.05) than the others at the total acceptability. Volatile compounds of the culture broth of Saccharomyces fermentati YK-19 were analysed by gas chromatography, and 6 species of esters and 4 species of alcohols were identified. Among them, the ester substances which broth largely responsible for the apple-like flavour in the sensory evaluation, were found to be ethyl 2-methyl butvrate, ethyl pentanoate and ethyl acetate.

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Breeding of Yeast Strain with Starch Utilizing and Alcohol Fermenting Ability by Protoplast Fusion (전분분해활성과 알코올 발효능을 보유한 효모의 육종)

  • Ju, Min-No;Hong, Sung-Wook;Kim, Kwan-Tae;Yum, Sung-Kwan;Kim, Gye-Won;Chung, Kun-Sub
    • Microbiology and Biotechnology Letters
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    • v.36 no.2
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    • pp.158-164
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    • 2008
  • The fusants which contain starch utilizing ability and alcohol fermenting ability were developed by protoplast fusion of Saccharomyces cerevisiae KOY-1 and Saccharomyces diastaticus KCTC 1804. Sacharomyces cerevisiae KH-12 was obtained by haploid induction from Saccharomyces cerevisiae KOY-1. The auxotropic mutants of yeast were obtained by using an ethylmethane sulfonate (EMS). The frequency of protoplast formation in Saccharomyces cerevisiae KOY-1 $(Met^-)$ and Saccharomyces diastaticus KCTC 1804 $(Trp^-)$ were 90.5% and 97.7%, respectively. The frequency of fusant formation was $1.79{\times}10^{-4 }$ for the regenerated protoplast and the 1,000 fusants were obtained. Fusant FA 776 was selected as a potential yeast which contain an alcohol fermenting ability in the starch medium. The genetic stability was 4.64% for 10 passages of generation. Fusant FA 776 produced 13mg/ml of alcohol in 24% starch medium and showed 1.86-fold higher alcohol fermenting ability than Saccharomyces diastaticus KCTC 1804.

The effect of the ammonium thiocyanate on the fermentation of the yeasts.(Saccharomyces brenerei-Hefe-Rasse XII, Saccharomyces formosensis No. 396 IAM) and Saccharomyces cerevisiae IAM) (Ammonium thiocyanate 농도가 주정효모(Saccharomyces brenerei Hefe-Rasse XII, Saccharomyces formosensis No. 396 IAM) 및 맥주효모(Saccharomyces cerevisiae IAM)의 발효작용에 미치는 영향)

  • 조운복;이상태
    • Korean Journal of Microbiology
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    • v.6 no.1
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    • pp.29-35
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    • 1968
  • There are many reports on the effect of salts to the fermentation of the yeasts, but there are a few reports on the effect of the ammonium thiocyanate(NH$_4CNS$) on the fermentation of the yeasts(Saccharomyces brenerei-Hefe-Rasse X11, Saccharomyces formosensis No. 396 IAM and Saccharomyces cerevisiae IAM). A study has been made on the effects of the ammonium thiocyanate on the fermentation of the above yeasts. The results of the research are as belows: 1) The addition of 0. 00001 mol. of ammonium thiocyanate makes the fermentation of the yeast(Sacch. formosensis No. 39 IAM) facilitate, but has not influenced the another yeast (Sacch. brenerei-Hefe-Rasse XII). 2) The addition of 0. 001 mol. of ammonium thiocyanate makes fermentation of Rases XII the fastest, but the on other yeast (Sacch. formosensis No - 396 IAM) was accelerated by the addition of 0. 1 mol., and it seems to have abnormal fermentation by the addition of 0. 0001 mol. The addition of ammonium thiocyanate(0. 00001-0. 001 mol.) inhibited the fermentatirn of the yeast(Sacch. cerevisiae IAM), but the concentration of 0. 1 mol. does not interrupt the fermentation of Sacch. cerevisiae IAM and increased the fermentation. 3) The order of effects to the fermentation of each yeast is Sacch. brenerei-Hefe-Rasse XII, Sacch. formosensis No. 396 IAM, Sacch. cerevisiae IAM.

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Characterization of Ethanol Fermentation with Wild Type Yeast Strains (야생 효모 종류에 따른 알코올 발효 특성)

  • Baek, Seong Yeol;Lee, You Jung;Kim, Myoung-Dong;Yi, Jae-Hyoung;Mun, Ji-Young;Yeo, Soo-Hwan
    • Microbiology and Biotechnology Letters
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    • v.43 no.3
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    • pp.227-235
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    • 2015
  • The objective of this study was to improve the quality of Korean rice wine with wild type yeast strains isolated from various traditional Korean fermented foods. Herein the fermentation and sensory characterization of wild yeast, for the purposes of brewing Korean rice wine, was investigated. 12 yeast strains were examined for their ethanol and glucose tolerance. In addition, the pH, soluble solids, acidity, amino acidity, ethanol content, organic acids, and volatile compounds were also studied for the alcoholic beverages made with the wild yeasts. Almost all Saccharomyces genera yeasts were showed to have a tolerance at 10% ethanol, but non-Saccharomyces genera yeasts displayed a low tolerance. The alcoholic beverages fermented by non-Saccharomyces yeasts demonstrated higher levels of soluble solids, titratable acidity, amino acids, and lower ethanol content, when compared with the alcoholic beverages fermented by Saccharomyces genera yeasts. The organic acid content, such as malic acid, acetic acid, and succinic acid, was seen to also be higher. The electronic nose was analyzed, and discriminant function analysis (DFA) was used for discriminating wild yeast strains. The DFA plots indicated a significant separation of Saccharomyces genera and non-Saccharomyces yeast strains. For volatile compounds, ethyl acetate from non-Saccharomyces yeasts, and ethanol from Saccharomyces genera yeast, a high area ratio was observed.

Physiological Characteristics and Ethanol Fermentation of Thermotolerant Yeast Saccharomyces cerevisiae OE-16 from Traditional Meju (메주에서 분리한 고온성 효모 Sccharomyces cerevisiae OE-16 의 생리적 특성과 알코올발효)

  • 김나미
    • The Korean Journal of Food And Nutrition
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    • v.12 no.5
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    • pp.490-495
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    • 1999
  • A thermotolerant yeast Saccharomyces cerevisiae OE-16 was isolated from traditional Meju was investigated on their physiological characteristics and ethanol fermentation ability. Saccharomyces cerevisiae OE-16 were able to grow up to 45$^{\circ}C$ and 40% of glucose. Saccharomyces cerevisiae OE-16 was also resistant to 15% of KCl 1,200ppm of Pb2+, Hg2+ and 500ppm of potassium sorbate. From 20% glucose media Saccharomyces cerevisiae OE-16 produced 83.4g per liter of ethanol at 3$0^{\circ}C$ and 9.5g per liter of ethanol at 4$0^{\circ}C$ for 72 hours.

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Study on Mixed Cultures of Lactobacillus acidophilus and Saccharomyces cerevisiae in Soymilk (대두유에서의 Lactobacillus acidophilus와 Saccharomyces cerevisiae의 혼합배양에 관한 연구)

  • 유주현;오두환;공인수;박영서;임홍철
    • Microbiology and Biotechnology Letters
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    • v.16 no.2
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    • pp.131-135
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    • 1988
  • Lactobacillus acidophilus KFCC12731 and Saccharomyces cerevisiae KFCC32017 were incubated together in soymilk and the conditions for acid production were investigated. The acid production of Lactobacillus acidophilus was much higher when this organism was incubated with Saccharomyces cerevisiae in soymilk than when it was incubated alone. Optimum acid production by the mixed cultures of Lactobacillus acidophilus and Saccharomyces cerevisiae was achieved with the following conditions; a temperature of 34$^{\circ}C$, a 3:7-8:2 (OD 660) ratio of Lactobacillus acidophilus to Saccharomyces cerevisiae at inoculum, a 1.5% level of sucrose fortification or a 2.0-3.0 % level of skim milk powder fortification and a culture time of 12 hours or more.

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대두유를 이용한 Lactobacillus casei와 Saccharomyces uvarum의 젖산발효 조건

  • 유주현;구본탁;염도영;이정수
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1986.12a
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    • pp.530.2-530
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    • 1986
  • 젖산균 Lactobacillus casei와 Saccharomyces uvarum을 사용하여 대두유중에서의 최적 젖산발효조건을 검토하였다. 젖산발효 중의 온도의 영향은 Saccharomyces uvarum과의 혼합배양에서 37-4$0^{\circ}C$가 가장 좋았다. 배양시간은 24시간일때, Lactobacillus casei 와 Saccharomyces uvarum 의 접종비는 2:1일때 산생성이 가장 좋았다. Sucrose와 skim milk의 혼합배양시 대두유의 젖산발효에 미치는 영향을 검토한 결과 sucrose의 경우 1.0% 첨가하였을 때 산생성은 높게 나타났으며 skim milk를 첨가했을 때는 첨가하지 않고 배양했을 때와 비교하여 별다른 차이를 보여주지 않았다.

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질산염이 Saccharomyces cerevisiae의 발효작용에 미치는 영향

  • 김상준
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1979.04a
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    • pp.115.3-115
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    • 1979
  • 중금속을 함유한 13종의 질산염을 명 농도별로 첨가하여 주정효모 Saccharomyces cerevisine의 주정생산과 발효작용에 미치는 영향을 조사하였다. 1. 일반적으로 중금속을 함유한 질산염은 그 첨가량이 0.0001mo1. 보다 고농도일수록 Saccharo-myces cerevisiae의 발효작용을 점차 억제하였다. 2. Nickel nitrate, chromium nitrate들의 0.0001 moi.의 첨가는 Saccharomyces cerevisiae의 alcohol 발효작용을 약간촉진시켰다. 3. Cadmium nitrate 0.001mo1. 이상, cupric nit-rate, nickel nitrate, cobalt nitrate 0.01mo1. 이상, 그리고 silver nitrate, mercurous nitrate, manganese nitrate, zinc nitrate, lead nitrate, chromiun nitrate, ferric nitrate, bismuth nitrate 0.1mol. 의 농도에서 Saccharomyces cerevisiae 의 발효작용은 완전히 조지되었다.

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