• Biomedical Science Letters
• /
• v.28 no.4
• /
• pp.276-283
• /
• 2022

Saccharina japonica (SJ), a brown algae, exerts various pharmacological effects, including anti-oxidant, immunemodulating and anti-cancer properties. This study aimed to determine the pharmacological mechanism of SJ on atopic dermatitis in vivo and in vitro. We investigated the pharmacological effects of SJ on 2, 4-dinitrochlrobenzene (DNCB)- induced atopic dermatitis clinical symptoms in mice. Additionally, we evaluated the effects of SJ on the inflammatory cytokine production and nuclear factor-κB (NF-κB) activation in HaCaT cells. The findings of this study demonstrated that SJ reduced the clinical symptoms of atopic dermatitis, such as skin dryness, erythema and eczematous, and serum histamine and IgE level in DNCB-induced atopic dermatitis model. Additionally, SJ inhibited the NF-κB activation in atopic dermatitis-like skin lesion and HaCaT cells. Collectively, this result suggests that SJ could be used as a therapeutic agent for skin inflammation, including atopic dermatitis.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.28 no.4
• /
• pp.23-33
• /
• 2020

Marine algae(Macro algae) are easily bio-degradable, and by-products are available as feed and fertilizer. The biomass of marine algae has higher CO2 absorption capacity than the wood system, and is highly valuable in use due to its fast growth speed and wide cultivation area without special cost for raw material production. In 2018, Marine algae production was 1,722,486ton, such as Saccharina japonica, Undaria pinnatifida and Porphyra tenera, the large amounts of by-products have been generated in the food processing facilities for commercialization. In this study, Saccharina japonica, Undaria pinnatifida were collected in the south coast region and Porphyra tenera was collected in the west coast region. The theoretical methane potential and biochemical methane potential(BMP) were analyzed, and Modified Gompertz model and Parallel first order kinetics model were adopted for the interpretation of the cumulative methane production curves. The theoretical methane potential of Saccharina japonica, Undaria pinnatifida and Porphyra tenera were 0.393, 0.373 and 0.435 N㎥/kg-VS, respectively. BMP obtained by the Modified gompertz model 0.226, 0.227, and 0.241 N㎥/kg-VS for Saccharina japonica, Undaria pinnatifida and Porphyra tenera, respectively. And BMP obtained by the Parallel first order kinetics model were 0.220, 0.243, and 0.240 N㎥/kg-VS for Saccharina japonica, Undaria pinnatifida and Porphyra tenera, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.47 no.3
• /
• pp.204-210
• /
• 2014

The brown alga Saccharina japonica is consumed as a foodstuff in many countries. Carbon tetrachloride ($CCl_4$) is a potent hepatotoxin that is used to assess hepatotoxicity in animal models. This study assessed the protective effect of S. japonica extract (SJE) on $CCl_4$-induced acute liver injury in rats. Experimental rats were divided into the following three groups: control, $CCl_4$, and $CCl_4$+SJE; the latter two groups were given 150 or 300 mg SJE/kg orally for 10 days. Three hours after the final treatment, all rats-except for those in the control group-were administered intraperitoneal injections of $CCl_4$. One day later, blood and liver samples were collected for evaluation biomarker of levels. Aspartate transaminase (APT; GPT) and alanine aminotransferase (ALT; GOT) levels were markedly lower in the $CCl_4$+SJE group than the $CCl_4$ group. The hepatic superoxide dismutase (SOD) activity of the $CCl_4$+SJE group was significantly lower than that of the $CCl_4$ group. In comparison, glutathione S-transferase (GSH) and catalase (CAT) levels were significantly higher in the $CCl_4$+SJE group. Western blotting revealed that SJE attenuated the $CCl_4$-induced EGFR and MAPK activity in the liver. Thus, we conclude that SJE protects against $CCl_4$-induced hepatotoxicity.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.45 no.4
• /
• pp.351-357
• /
• 2012

To determine whether the seaweed Saccharina japonica can effectively utilize dissolved nutrients from Sebastes schlegeli fish cultures, a laboratory experiment was conducted in a static system for 7 days at ESFRI, NFRDI in Korea. The experiment included an S. schlegeli monoculture system and an S. schlegeli-S. japonica IMTA system. Saccharina schlegeli density ($415{\pm}24g$; mean${\pm}$SE) remained the same in all treatments, whereas seaweed density varied across treatments of 0, 0.5, 1, 2, and 3 kg (control and T1-T4, respectively). During the experiment, nutrient ($NH_4^+$ and $PO_4^{3-}$) concentrations were measured at 24-h intervals. $NH_4^+$ concentration of the control group increased from $0.117{\pm}0.021mg/L$ at the start of experiment to $5.836{\pm}0.904mg/L$ at the end of experiment. $NH_4^+$ concentrations of each treatment were $3.004{\pm}0.040$, $2.086{\pm}0.133$, $1.642{\pm}0.121$ and $0.775{\pm}0.007mg/L$ in T1, T2, T3, and T4, respectively, at the end of experiment. The concentration of $PO_4^{3-}$ exhibited a similar trend to $NH_4^+$ concentration. $NH_4^+$ and $PO_4^{3-}$ concentrations significantly decreased with increased S. japonica thallus density each day (P<0.05). The nutrient removal efficiency (NRE) and nutrient uptake rate (NUR) showed different relationships with changes in thallus density; NRE increased but NUR decreased as thallus density increased. Based on measured concentrations of $NH_4^+$ and S. japonica weight, regression analysis defined the relationship between as an exponential function, $Y=3.8165e^{-0.505X}$ ($R^2$ = 0.9552). Our results demonstrated that S. japonica can function as an efficient component in IMTA with environmental and potentially economic benefits for fish hatcheries.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.53 no.3
• /
• pp.351-360
• /
• 2020

Previous studies have suggested that microbial fermentation is an attractive process to develop food products using seaweed. The objective of this study was to isolate and characterize lactic acid bacteria (LAB), which are used as starters for seaweed fermentation. The isolation of LAB strains was conducted using kelp Saccharina japonica kimchi, a well-known fermented seaweed in southeastern Korea. Based on the assay of acid tolerance, bile tolerance and antioxidant activity, 15 strains of LAB were selected for further study. The LABs exhibited cholesterol lowering activity in the range of 15.50 to 94.77%. Among the LABs suitable for food production, Lactobacillus plantarum D-11 had the highest antioxidant and cholesterol lowering activities. This probiotic strain will be applied to develop various kelp fermentation products.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.53 no.3
• /
• pp.361-367
• /
• 2020

The objective of this study was to investigate the effect of lactic acid bacteria (LAB) fermentation on the antioxidant activity of kelp Saccharina japonica water extract. Three LAB strains that had exhibited superior antioxidant activity in a previous study were selected for the kelp fermentation starter. The antioxidant activity of the fermented extracts was analyzed during fermentation. After 48 h of fermentation, the extract-fermented Lactobacillus plantarum D-11 strains showed the highest antioxidant activity in terms of DPPH (2,2-diphenyl-2-picryl hydrazyl) radical scavenging, ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] radical scavenging, oxygen radical absorbance capacity (ORAC) and fluorescence recovery after photobleaching (FRAP) assay. Furthermore, the analysis of total phenolic and flavonoid contents revealed that the enhanced antioxidant activity was mainly due to the increased antioxidant content from fermentation. Thus, this study suggests that probiotic LAB fermentation is an attractive approach for the development of various kelp fermentation products.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.46 no.4
• /
• pp.359-364
• /
• 2013

Inordinate stress causes disorders of various systems in humans and activates defense mechanisms to maintain homeostasis in the body. Sleep is a vital, highly organized process regulated by complex systems of neuronal networks and neurotransmitters. Sleep is an essential biological process whose underlying regulating involves numerous anatomical structures and biochemical substances that can be compromised by stress and by the immune system. Gamma-amino butyric acid (GABA) is the main inhibitory neurotransmitter of the central nervous system, and activation of GABAA receptors is known to favor sleep. This study was conducted to evaluate the possible application of Lactobacillus brevis BJ20 fermentation to improve the functional qualities of sea tangle Saccharina japonica and oyster Crassostrea gigas. Antioxidant activity was determined by assaying levels of radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide. L. brevis BJ20 fermentation of sea tangle and oyster enhanced both antioxidant and antiinflammatory activities. These results suggested that L. brevis BJ20 fermented sea tangle and oyster could be used for alleviation of stress and to promote sleep.

• Fisheries and Aquatic Sciences
• /
• v.20 no.1
• /
• pp.1.1-1.7
• /
• 2017

A survey was conducted to investigate the presence of epiphytic organisms in four kelp Saccharina japonica farms in the coastal area of Korea from 2014 to 2015. Of 740 kelp samples that were taken, 208 exhibited six kinds of epiphytic organisms, including hydroid (detection rate: 11.6%), bryozoan (6.4%), polychaete (3.4%), algae (3.2%), caprellid (3%), and oyster (0.5%). The infestation rate for hydroid, bryozoan, and polychaete was significantly higher in the Wando farm, Busan farm, and Pohang farm, respectively. Epiphytic organisms were generally observed during May to September and not January to April, indicating that their infestation was significantly higher when the water had a higher temperature. The histopathogical examination revealed that hydroid and bryozoan organisms were attached on the cuticula of the thallus while some algae were attached on the cuticula of the thallus or had penetrated the epidermis. These results indicate that hydroid and bryozoan were the most predominant epiphytic organisms in Korean kelp farms, even though the infested thallus had not been broken.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.55 no.4
• /
• pp.408-416
• /
• 2022

This study investigated the physicochemical properties and physiological activities of jelly prepared from gelatin, sugar, bokbunja extract, and different amounts (0, 0.5, 1, 2 and 3%) of fermented sea tangle Saccharina japonica powder (FSP). The jelly moisture, pH, and sugar content slightly increased with increasing the FSP content. Hardness, springiness, gumminess, chewiness, and cohesiveness also increased with increasing FSP concentration. Jelly antioxidant activity did not change significantly with increasing FSP. In contrast, alcohol dehydrogenase and aldehyde dehydrogenase activities in the jellies increased significantly with increasing FSP concentration. β-secretase inhibitory activity in jellies also increased with increasing FSP concentration. Jellies containing 0.5 or 1% FSP achieved the highest overall sensory acceptance scores. Taken together, these data indicate that addition of FSP to jelly appears to improve its quality and physiological activities.

• ALGAE
• /
• v.33 no.1
• /
• pp.101-108
• /
• 2018

Saccharina japonica was introduced to both Korea and China from Hokkaido, Japan, and it has become an economically important species in both nations. After a long period of cultivation, several varieties of S. japonica have been developed in Korea and China. In this study, we conducted aquacultural research on the persistence of thalli from two kelp cultivars, one from China (Huangguan No. 1) and one from Korea (Sugwawon No. 301), between December 2015 and November 2016 in Haenam, Korea. The maximum length was $247.8{\pm}13.0$ and $227.5{\pm}42.0cm$, respectively, which were significantly longer in Sugwawon No. 301 than in Huangguan No. 1. The maximum width was $29.9{\pm}5.4$ and $23.2{\pm}1.9cm$, respectively, which were significantly wider in Huangguan No. 1 than in Sugwawon No. 301. The mean biomass obtained from the culture ropes was for Sugwawon No. 301 was $3.5{\pm}0.3kg\;wet\;wt\;m^{-1}$ and for while Huangguan No. 1 was $3.1{\pm}1.0kg\;wet\;wt\;m^{-1}$ of culture rope. After August, the persistence of the thalli of Sugwawon No. 301 was two months longer than that of Huangguan No. 1. We found that the Sugwawon No. 301 performed as well as the Huangguan No. 1 in Korean waters possibly due to increased flexibility as a result of the different cell arrangements of the two cultivars. Overall, the use of the Sugwawon No. 301 cultivar rather than the Huangguan No. 1 cultivar of S. japonica appears the best alternative to help to ensure a stable year round algal feed supply for the Korean abalone industry.