• BMB Reports
• /
• v.39 no.4
• /
• pp.432-440
• /
• 2006

A tuber lectin from Arisaema jacquemontii Blume belonging to family Araceae was purified by employing a single step affinity chromatography using column of asialofetuin-linked amino activated silica beads and the bound lectin was eluted with 100 mM glycine-HCl buffer pH 2.5. The purified A. jacquemontii lectin (AJL) showed a single protein band with an apparent molecular mass of 13.4 kDa when submitted to SDS-polyacrylamide gel electrophoresis under reducing as well as non-reducing conditions. The native molecular mass of AJL determined by gel filtration on a Biogel P-200 column was 52 kDa and its carbohydrate content was estimated to be 3.40%. Thus AJL is a tetrameric glycoprotein. The purified lectin agglutinated erythrocytes from rabbit but not from human. Its activity was not inhibited by any of the mono- and disaccharides tested except N-acetyl-D-lactosamine having minimal inhibitory sugar concentration (MIC) 25 mM. Among the glycoproteins tested only asialofetuin was found to be inhibitory (MIC $125\;{\mu}g/mL$). A single band was obtained in native PAGE at pH 4.5 while PAGE at pH 8.3 showed two bands. Isoelectric focusing of AJL gave multiple bands in the pI range of 4.6-5.5. When incorporated in artificial diet AJL significantly affected the development of Bactrocera cucurbitae (Coquillett) larvae indicating the possibility of using this lectin in a biotechnological strategy for insect management of cucurbits. Larvae fed on artificial diet containing sub-lethal dose of AJL showed a significant decrease in acid phosphatase and alkaline phosphatase activity while esterase activity markedly increased as compared to larvae fed on diet without lectin. Out of various human cancer cell lines employed in sulphorhodamine B (SRB) assay, this lectin was found to have appreciable inhibitory effect on the in vitro proliferation of HCT-15, HOP-62, SW-620, HT-29, IMR-32, SKOV-3, Colo-205, PC-3, HEP-2 and A-549 cancer cell lines by 82, 77, 73, 70, 41, 41, 37, 29, 21 and 21% respectively.

• Journal of Technology Innovation
• /
• v.23 no.3
• /
• pp.341-372
• /
• 2015

While creativity and innovation is the key to drive the creative economy in the South Korea, the development of analysis framework to evaluate the size and performance is limited. The present study suggests a framework and a method to assess economic impact of the creative economy using inter-industry analysis which employs input-output efficiencies populated by the Korean Bank and empirical data from the national informatization survey conducted by the National Information-society Agency(NIA). The results indicated that, as of 2013, despite of economy downturn, the creative innovation based on the information communication technology(ICT) had been significantly led the production, value-added, and employment inducement. The effect is predominant in the creative industry in an broad sense, that is, technology intensive manufacturing industry. Theoretical and policy implications are discussed.

• The Journal of the Petrological Society of Korea
• /
• v.21 no.3
• /
• pp.367-383
• /
• 2012

The Gyeongsang Arc is the most notable of the Korea Arc that is composed of several volcanic arcs trending to NE-SW direction in the Korean peninsula. The Hayang Group has many volcanogenic interbeds of lava flows by alkaline or calc-alkaline basaltic volcanisms during early Cretaceous. Late Cretaceous calc-alkaline andesitic and rhyolitic volcanisms reconstructed the Gyeongsang Arc that consist of thick volcanic strata on the Hayang Group in The Gyeongsang Basin. The volcanisms characterize first eruptions of basaltic and andesitic lavas with small pyroclastics, and continue later eruptions of dacitic and rhyolitic ash-fall and voluminous ash-flow with some calderas and then domes and dykes. During the Early Cretaceous (about 120 Ma), oblique subduction of the Izanagi plate to NNW from N direction results in sinistral strike-slip faults to open a pull-apart basin in back-arc area of the Gyeongsang Arc, in which erupted lava flows from generation of magma by a decrease in lithostatic pressure. Therefore the Gyeongsang Basin is interpreted into back-arc basin reconstructed by a continental rifting. Arc volcanism began in about 100 Ma with exaggeration of the back-arc basin in the Gyeongsang, and then changed violently to construct volcanic arcs. During the Late Cretaceous (about 90 Ma), orthogonal subduction of the Izanagi plate to NW from NNW direction ceased development of the basin to prolong violent volcanisms.

• Journal of the Korea Society of Computer and Information
• /
• v.17 no.12
• /
• pp.187-198
• /
• 2012

International standard for software quality evaluation, ISO/IEC 9126 defines the quality characteristics and sub-characteristics consisting of quality of the software. Also, in this standard, the quality assessment items are presented in metrics based on maturity, fault tolerance, recoverability and compliance which are sub-characteristics of reliability. However, this reliability assessment items which are presented are not applied now due to the mathematical difficulties and the problems caused by collection of data. In order to resolve these problems, this study introduces measures to develop a specific test technique by quality attributes and to measure the quality. In other words, the detailed guidelines are presented to be utilized as a standard by summarizing test activities and techniques by each development life the most commonly used. The SW quality results are derived by assigning weights to software in order of the most important quality characteristics of respective software using checklist that can be easily utilized and applying test technique that can be utilized for detailed evaluation items and checklist. This improves problems that include reliability assessment item of ISO/IEC 9126 as the measures to measure new reliability quality of software.

• KEPCO Journal on Electric Power and Energy
• /
• v.6 no.3
• /
• pp.285-288
• /
• 2020

The demand for coating technology on electric power equipment that has arisen from such issues regarding the attaching of illegal advertisements and posters to electric power distribution boxes such as TR, SW, etc. in down town areas seeks to produce functional coating surfaces using polymers and nano-materials that will result in improvements in self-cleaning performance and greater stability even under harsh environmental conditions. KEPCO-coatings consist of copolymerized acrylic resin and methacryl-modified reactive silicone that are able to chemically combine, which results in performance improvement without any leakage of of silicone, thus contributing to its properties of high-stability. Thus, the research team has also started long-term on-site testing on 9 electric power distribution spots around a city center in cooperation with the KEPCO Daeduck-yusung branch. The KEPCO-coating technology could advance the best coating materials and processes to meet appropriate circumstances for a variety of outdoor damage environment. It is also predicted that KEPCO could be possible to expand international electric maintenance markets and to arrange business platforms if KEPCO would achieve its original technology (IPs) by the means of upgrading in self-cleaning coating technology and obtaining long-term on-site test records from nationwide electric facilities.

• Journal of Science Education
• /
• v.44 no.2
• /
• pp.183-196
• /
• 2020

This study explores the effects of STEAM program using Arduino on preservice science teachers toward their STEAM core competencies. The STEAM program using Arduino consists of four stages: presentation of situation, creative design, emotional touch, and evaluation. The preservice science teachers learned the theoretical backgrounds of STEAM and Arduino. Then, they were given the chance to think about an environmental issue, which is fine dust. The preservice teachers designed an air cleaner and a fine dust measuring instrument using Arduino. The preservice science teachers also produced the air cleaner and the measuring instrument using Arduino. They measured the level of fine dust in the classroom before and after the use of the air cleaner. That is, the preservice teachers experienced each stage of STEAM: seriousness of fine dust, design and production of the measuring instrument of fine dust and air cleaner, and evaluation of the effectiveness of air cleaner. Further, they reflected on their experiences of STEAM program using Arduino. The results indicate that these preservice science teachers statistically improved communication competency, problem-solving competency, gathering information competency, logical analytical thinking competency, and creativity competency. However, there were no statistical improvements on teamwork competency and self-development competency. This study suggests that experiencing STEAM program using Arduino is valuable for the preservice science teachers to develop STEAM core competencies and further implement STEAM program their science classes in the future.

• Proceedings of the KOSOMBE Conference
• /
• v.1996 no.11
• /
• pp.129-134
• /
• 1996

1차 년도 G-7 개발 과제로 수행된 자기 공명 진단 장치 (Magnetic Resonance Imaging System)의 개발 내용을 간략히 소개하였다. 성공적인 IT Compact 자기 공명 진단 장치의 완성을 위해 일차적으로 (1)RF (고주파), Gradient(경사 자계), Spectrometer 등의 Hard-ware 관련 MRI 핵심부분, (2) RF, Gradient, Spectrometer, Magnet 등의 각 Sub-system을 연결, 조합, 조정하여 하나의 체계적인 시스템으로 통합하고 운영하는 과정(System Integration), (3)사용자와 시스템을 연결하는 User Interface, Data Base Management, Real time 운영 SW 등과 (4)임상에 적용하여 구체적인 성능과 효용성을 확인하는 기술 등에 대하여 집중 연구하였다. 개발 방법은 (1)지난 16년간 국내에 축적 된 연구 개발 인력들을 최대한 활용하고 (2)연구 개발을 국제화 시켜 필요한 경우 부분별로 개발 인력을 해외에서 보완하고 (3)소수 정예 전문 인력 주의와 요소 기술 또는 중요 부품을 경쟁성 검토 후 필요 시 Out-sourcing 활용으로 최저의 비용으로 개발 기간을 최소화 하는 데 두었다. 개발된 1.0Tesla자기 공명 영상 장치는 미국 물리 학회에서 규격화한 Phantom및 임상 적용을 통하여 서울대 의대 연구 팀과 지속적으로 성능을 평가해 왔다. 개발된 시스템의 해상도는 $256{\times}256$ head 영상에서 1mm 이 하의 해상도를 가짐을 resolution phantom 을 통하여 확인할 수 있었고, $512{\times}512$ 영상에서 는 약 0.5 mm 의 물체를 분리 해냄으로써 외제 시스템들 보다 우수하게 평가 되었다. 차폐 경사코일의 Eddy current영향은2%이내로 촬영 시 영향은 거의 무시할 수 있었다. 또한, 개발된 영상 기법들, 즉 Multislice/Multi Echo, Oblique angle imaging, 64 Echo train을 갖는 고속 촬영 기술들이 자기 공명 장치에 장착되어 임상 적용에 문제가 없도록 하였다. 또한 20mT/m/Amp의 강력한 능동 차폐 경사 자계 코일(Active Shield Gradient Coil)을 기본 사양으로 하고, 수신단을 최대 6개로 확장토록 하여 2차년도의 초고속 촬영 기법(EPI) 및 Phased Array 코일 촬영이 가능토록 하였다. 1차 년도 개발 과제 수행 결과와 향후 개발 과제를 바탕으로 최종 목표인 국제 경쟁력이 있는 자기 공명 진단 장치 즉 기능과 영상의 질은 선진국 제품과 동일하거나 우수하되, 저가격을 구현한 상용화 제품이 완성되어, 첨단 의료기기로서 산업 구조 고도화에 기여하고 수입대체 뿐만 아니 라 수출을 통한 국익 창출과 국가의 기술을 통한 위상 제고에 기여되길 기대한다.

• Korean Journal of Medicinal Crop Science
• /
• v.25 no.5
• /
• pp.328-334
• /
• 2017

Background: In this study, we evaluated the anti-cancer activity and potential molecular mechanism of 70% ethanol extracts of the root of Aralia cordata var. continentalis (Kitagawa) Y. C. Chu (RAc-E70) against human colorectal cancer cells. Methods and Results: RAc-E70 suppressed the proliferation of the human colorectal cancer cell lines, HCT116 and SW480. Although RAc-E70 reduction cyclin D1 expression at the protein and mRNA levels, RAc-E70-induced reduction in cyclin D1 protein level occurred more dramatically than that of cyclin D1 mRNA. The RAc-E70-induced downregulation of cyclin D1 expression was attenuated in the presence of MG132. Additionally, RAc-E70 reduced HA-cyclin D1 levels in HCT116 cells transfected with HA-tagged wild type-cyclin D1 expression vector. RAc-E70-mediated cyclin D1 degradation was blocked in the presence of LiCl, a $GSK3{\beta}$ inhibitorbut, but not PD98059, an ERK1/2 inhibitor and SB203580, a p38 inhibitor. Furthermore, RAc-E70 phosphorylated cyclin D1 at threonine-286 (T286), and LiCl-induced $GSK3{\beta}$ inhibition reduced the RAc-E70-mediated phosphorylation of cyclin D1 at T286. Conclusions: Our results suggested that RAc-E70 may downregulate cyclin D1 expression as a potential anti-cancer target through $GSK3{\beta}$-dependent cyclin D1 degradation. Based on these findings, RAc-E70 maybe a potential candidate for the development of chemopreventive or therapeutic agents for human colorectal cancer.

• Journal of the Geological Society of Korea
• /
• v.54 no.6
• /
• pp.615-630
• /
• 2018

Basin analysis is a research field to understand the formation and evolution of sedimentary basins. This task requires various geoscientific datasets as well as numerical and graphical modelling techniques to synthesize results dimensionally in time and space. For basin analysis and modelling in a comprehensive workflow, BasinVis 1.0 was released as a MATLAB-based program in 2016, and recently the software has been extended to BasinVis 2.0, with new functions and revised user-interface. As a case study, this work analyses the southern Vienna Basin and visualizes the sedimentation setting and subsidence evolution to introduce the basin modelling functions of BasinVis 2.0. This is a preliminary study for a basin-scale modelling of the Vienna Basin, together with our previous studies using BasinVis 1.0. In the study area, during the late Early Miocene, sedimentation and subsidence are significant along strike-slip and en-echelon listric normal faults. From the Middle Miocene onwards, however, subsidence decreases abruptly over the area and this situation continues until the Late Miocene. This is related to the development of the pull-apart system and corresponds to the episodic tectonic subsidence in strike-slip basins. The subsidence of the Middle Miocene is confined mainly to areas along the strike-slip faults, while, from the late Middle Miocene, the depocenter shifts to a depression along the N-S trending listric normal faults. This corresponds to the regional paleostress regime transitioning from NE-SW trending transtension to E-W trending extension. This study applies various functions and techniques to this case study, and the modelled results demonstrate that BasinVis 2.0 is effective and applicable to the basin modelling.

• Korean Journal of Plant Resources
• /
• v.32 no.5
• /
• pp.589-614
• /
• 2019

To investigate the distribution of vascular plants growing at Mt. Guryong, we surveyed from March in 2016 to October in 2017. The flora of Mt. Guryong was classified as a total of 526 taxa comprising of 87 families, 298 genus, 449 species, 4 subspecies, 63 varieties, 10 forma. Among them, the endemic plants in Korea were investigated as 10 taxa of including Pseudostellaria coreana (Nakai) Ohwi. The Korean rare plants species were investigated as 16 taxa, among them degree of CR investigated as 1 taxa of Cypripedium macranthum Sw., VU investigated as 5 taxa of including Rhododendron micranthum Turcz., degree of LC investigated 9 taxa of including Clematis koreana Kom., degree of DD investigated 1 taxa of Adenophora grandiflora Nakai. Endemic plants were investigated as 14 taxa of including seudostellaria coreana (Nakai) Ohwi and The floristic special plants were a total of 84 taxa such as 2 taxa in degree V(in cluding Polypodium virginianum L.), 7 taxa in degree IV (including Anemone reflexa Steph. & Willd.), 21 taxa in degree III(including Celtis aurantiaca Nakai), 22 taxa in degree II (including Thelypteris phegopteris (L.) Sloss.) and 31 taxa in degree I (including Cimicifuga simplex (DC.) Turcz.) was carried out to provide basic data. The naturalized plants were investigated as 37 taxa of including Chenopodium ficifolium Smith., Thlaspi arvense L., Rumex crispus L. and the percentage of urbanization index was 11.53%, the naturalized ratio was 6.99% respectively.