• Nutrition Research and Practice
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• 제1권2호
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• pp.100-104
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• 2007

Saururus chinensis Baill was reported to inhibit ${\alpha}-glucosidase$ in vitro and flatten postprandial increase in blood glucose in streptozotocin (STZ)-induced diabetic rats. We studied the effect of chronic consumption of S. chinensis Baill on blood glucose and lipid profile in STZ-induced diabetic male rats fed high fat diet. Male rats weighing 100-120 g were fed 30% fat diet with and without 10% freeze-dried leaves of S. chinensis Baill for 7 weeks after 1 week of adaptation. The rats were rendered diabetic by intravenous injection of STZ (60 mg/kg) after 6-week feeding of the assigned diets. At 1 week after the injection, the rats were sacrificed after an overnight fast. Plasma glucose ($380.2{\pm}14.4mg/dL$), total cholesterol ($93.9{\pm}7.9mg/dL$) and triglyceride levels ($123.6{\pm}7.5mg/dL$) of the S. chinensis Baill group were significantly lower than those of the control group ($418.1{\pm}12.0mg/dL,\;119.9{\pm}9.4mg/dL,\;152.0{\pm}10.3mg/dL$, respectively, p<0.05). Chronic consumption of S. chinesis Baill significantly decreased maltase activity of the small intestinal mucosa ($120.1{\pm}8.7U/g$) protein compared with the control group ($96.8{\pm}7.0U/g protein, p<0.05). These results suggest that S. chinensis Baill have hypoglycemic and hypolipidemic effects by inhibiting ${\alpha}-glucosidase$ activity in the animal model of diabetes mellitus.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.121-121
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• 2019

In Islamic dietary guidelines, Halal foods are allowed as edible blessed food. Most foods were categorized within halal for Muslims. The main point of Halal food is that foods are clean in every process and based on Halal standard which might be different in each country. Most pancreatic ${\beta}$ cells synthetize, store, and release insulin. Specific molecular, functional as well as ultrastructural traits of pancreatic ${\beta}$ cells could control their insulin secretion properties and survival phentoype. Insulin-secreting pancreatic ${\beta}$-cells are essential regulators of mammalian metabolism. In addition, the pancreatic ${\beta}$ cell plays an important role in the pathogenesis of type 1 and type 2 diabetes as improving glucose homeostasis by preserving, expanding and improving the function of this key cell type. However, the pharmacological effect of halal food has not been unclear yet, especially food habit-dependent diabetes. The aim of the this study was to determine the preventive effect of Iran plants extract (Almond, Garlic, Cumin, Ginkgo biloba, Holy basil, Psyllium, Satureja khuzistanica, Fenugreek, Green tea, Ipomoea betatas, Blueberry) on RINm5F cells and MIN6 cells as pancreatic ${\beta}$ cell line. The cytotoxicity of the extracts of Iran plants on RINm5F cells and MIN6 cells were measured by using MTT assays. The preventive effects of Iran plant extracts were measured by WST-8 cell proliferation assay on streptozotocin (STZ)-induced cell death in MIN6 cells. In presented result showed that all extract of Iran plants (0.01-10mg/ml) did not show cytotoxicity in RINm5F cells and MIN6 cells. Among non-cytotoxic extract, the protective effects could be detect in high dose concentration. These results suggest that the extract of Iran plants may serve as a potential therapy for diabetes.

• Journal of Nutrition and Health
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• 제56권1호
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• pp.24-34
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• 2023

상지 물추출물의 투여는 STZ 유도 당뇨 마우스의 공복 혈당, 혈중 총 CHO, LDL-CHO, 간 무게 및 간 조직 TG를 NC군 수준으로 감소시키고 혈장 총 CHO대비 HDL-CHO비는 NC군 수준으로 증가시켰으며, 소장 proximal region의 이당류 분해효소인 lactase, sucrase 활성을 억제하였다. 이는 상지를 차로 우려내어 음용할 경우 당뇨 예방 및 증상 개선의 가능성을 기대해 볼 수 있음을 제시한다. 상지는 국내산 뽕나무 부산물로 대량 생산될 뿐만 아니라 식품의 원료로 사용이 가능한 소재이므로 향후 상지 물추출물의 생리활성성분 분리 및 혈당조절기전 연구를 보다 깊이 수행한다면 안전하고 효과적인 고부가가치 혈당조절 기능성식품 개발의 기초자료로 활용될 수 있을 것이다.

• 한국식품영양과학회지
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• 제33권10호
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• pp.1618-1625
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• 2004

본 연구에서는 임실특산물인 쥐눈이콩및 쥐눈이식초콩의 혈당강하 및 인슐린 감수성에 대한 효과를 검정콩, 메주콩과 비교하여 조사하였다. 식이물질의 성분검사 결과 쥐눈이콩과 쥐눈이식초콩이 검정콩과 메주콩에 비하여 당뇨개선의 효과가 있는 여러 아미노산 및 식이섬유 함량이 유의적으로 높게 측정 되었다. 실험 을 위 해 평균체중 195.5$\pm$0.98 g의 숫컷쥐를 정상군(NC), 당뇨대조군(DI, DC), 검정콩식이군(DB), 메주콩식이군(DY), 쥐눈이콩식이군(DS), 쥐눈이식초콩식이군(DSS)으로 총 7개군으로 나누어 해당식이첨가 시료로 4주간 사육하였다. 당뇨유발을 위 해 STZ를 1회 (70 mg/kg/rat) 투여하였으며 그 결과 체중변화는 당뇨대조군(DC)에서 21.12 g감소함에 반하여 각각의 시료를 첨가식이한 당뇨실험군에서 특히 쥐눈이콩(DS)과 쥐눈이식초콩(DSS) 식이군은 높은 식이 이용 효율(FER)을 보여 각각 14.73$\pm$3.65 g 및 16.71$\pm$5.54 g의 몸무게 증가를 보였다. 음용수 및 뇨량의 측정 결과 역 시 당뇨실험군에서 유의적인 감소(p<0.05)를 보였다. 혈당량은 당뇨대조군(DC)에 비하여 검정콩(DH),메주콩(DY), 쥐눈이콩(DS), 쥐눈이식초콩(DSS) 식이군이 각각 17.9%, 16.9%, 10.35%, 19.54%씩 유의하게 감소하였으나 정상대조군(NC)보다는 높은 수치를 나타내었다. 또한 당뇨쥐의 인슐린 감수성에 대한 관찰결과 당뇨대조군 중 인슐린 무처지군(DC)에 비하여 모든 당뇨실험군에서 높은 값을 보였다. 결론적으로 임실산 쥐눈이콩과 쥐눈이식초콩은 당뇨쥐의 혈당강하 및 인슐린감수성에 대한 개선작용의 가능성이 있으므로 당뇨합병증 예방의 기능성 식품으로 기대된다.

• Journal of Nutrition and Health
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• 제42권1호
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• pp.5-13
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• 2009

최근 당뇨환자에게서 산화적 손상이 증가됨이 보고되면서 항산화식품의 섭취를 통한 DNA 손상 개선노력이 다각도로 시도되고 있다. 차가버섯을 비롯한 여러 가지 버섯류에 항산화활성 및 혈당강하 효과가 있음이 알려지고 있으므로 본 연구에서는 streptozotocin (STZ) 유발 당뇨쥐를 사용하여 차가버섯에 항산화 효과 및 DNA 손상 억제효과가 있는지, 그리고 이런 항산화효과가 당뇨쥐의 혈당을 감소시키는지를 알아보았다. Sprague-dawley종 수컷 흰쥐(4주령, $110{\pm}6\;g$) 32마리를 대조군, 당뇨군, 저농도 차가버섯투여군 (0.5 g/kg BW) 및 고동도 차가버섯투여군 (5 g/kg BW) 등 4군으로 나누어 동물실험을 진행하였으며, 꼬리 정맥에 STZ을 주사하여 당뇨를 유발시킨 후 4주간 사육하였다. 4주 후에 혈액과 간을 채취하여 혈장 glucose, 혈장 총항산화능 (total radical-trapping antioxidant potential, TRAP) 수준 및 ${\alpha}$-tocopherol 수준, 적혈구 catalase, superoxide dismutase (SOD), 및 glutathione peroxidase (GSH-Px)의 활성도, 그리고 임파구 및 간조직의 DNA 손상 정도를 comet assay로 측정하였다. 실험 결과, 대조군에 비해 당뇨군에서 혈당이 상승하고 (p < 0.05) 혈장 TRAP 수준은 감소하였으며 (p < 0.05) 임파구의 DNA 손상은 증가하였고 (p < 0.05) 나머지 지표들은 차이가 나타나지 않았다. 당뇨군에 비해 저농도 및 고농도 차가버섯 투여군들에서 혈당이 감소하는 경향이 있었고 혈장 TRAP 수준과 ${\alpha}$-tocopherol 수준이 증가하는 경향을 보였으나 모두 유의적인 차이는 아니었으며, 당뇨군에 비해 차가버섯 투여군에서 tail length로 본 임파구 DNA 손상 정도가 유의적으로 감소하였다 (p < 0.05). 나머지 지표들은 당뇨군과 차가버섯 투여군들 간에 차이를 보이지 않았다. 본 연구결과, 차가버섯 열수추출물을 고농도 (5 g/kg BW)로 4주 동안 당뇨유발 흰쥐에게 투여하였을 때 혈당강하 효과는 유의적으로 나타나지 않았으나 당뇨로 인해 유발 된 DNA 손상정도가 정상수준까지 뚜렷하게 감소함을 관찰하였으며 이를 통해 부분적으로 차가버섯의 항산화 효과를 관찰할 수 있었다. 이와 같은 결과는 앞으로 차가버섯을 이용하여 당뇨환자의 DNA손상을 개선시키기 위한 건강기능식품 개발 연구에 활용될 수 있을 것으로 생각된다.

• Nutritional Sciences
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• 제6권3호
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• pp.127-134
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• 2003

This study investigates the effects of a raw diet (RD) on blood glucose and lipid metabolism in non-diabetic (normal) and streptozotocin (STZ)-induced diabetic rats. Male Sprague-Dawley vats were assigned to four groups (normal control, normal RD, diabetic control, and diabetic RD), for the four-week experimental period. The control groups were fed the AIN diet and the RD groups were fed a diet consisting only of raw materials. Weight gain was statistically lower in the RD group than the control. fasting plasma glucose was significantly lower in the diabetic RD group compared to the diabetic control group. The levels of triglycerides (TG), and of total cholesterol (TC) and LDL-cholesterol in the plasma, were lower in the RD groups than the control groups, but not significantly. There was a statistically significant decrease in the levels of TG and TC in the livers of the diabetic RD group, compared to the diabetic control group. The fecal levels of total lipids, TG, and TC were significantly higher in the RD groups, compared to the non-RD groups. It can be postulated that this raw diet may possess substantial hypoglycemic/hypolipidemic properties in diabetic rats.

• 한국환경보건학회지
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• 제31권1호
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• pp.47-54
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• 2005

To evaluate the hypoglycemic effect of propolis, streptozotocin (STZ) induced diabetic rats were divided into 4 groups such as, diabetic control group, low dose of propolis (0.1 ml) group, medium dose of propolis (0.3 ml) group and high dose of propolis (0.9 ml) group and feeded with propolis extracts for 30 days. After experiment, oral glucose tolerance test (Oral GTT) was carried, and 16 hours fasting blood sugar levels, body weights, blood lipid levels were measured. Finally, pancreatic histopathological study was performed. In conclusion, the propolis is effective to the treatment diabetes due to the reduction of the blood sugar level and the regeneration of the damaged $\beta$-cells shown in streptozotocininduced diabetic rats.

• 생약학회지
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• 제25권1호
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• pp.41-46
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• 1994

The ether, ethylacetate and n-butanol soluble fractions from the roots of Scutellaria baicalensis showed a significant inhibition of lens aldose reductase (AR) activity in vitro. During systematic fractionation of the active fractions, 7 flavonoids were isolated and compared their inhibitory activities against rat AR using DL-glyceraldehyde as a substrate, among which baicalin (VII) was found to exhibit the most potent inhibitory activity. Baicalin (VII) and wogonin-7-O-glucuronide (VI), with repeated treatments (30 mg/kg/day, i.p.) throughout the experimental periods caused a significant suppression of cataract formation induced by galactose (40 g/kg/day) as well as the decrease of galactitol accumulation in the rat lens. The flavonoids also exhibited a significant inhibition of sorbitol accumulation in the lenses of diabetic rats induced by streptozotocin (STZ).

• 동의생리병리학회지
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• 제22권6호
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• pp.1537-1543
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• 2008

The purpose of this study was investigation to examine the prevention diabetic mellitus and treatment effect on Gastrodia Elata Blume(GEB) dieted at prior and after induced diabetic application. Prior induced diabetic 3 weeks ago application GEB dieted. It is to analysis changes in body weight, blood glucose, SOD, CAT and histopathological findings. For the fingding significantly concentration diabetic rats were divided into 3 different experimental groups and each groups were induced diabetic. Experimental group Ⅰ (STZ-induced diabetic rats; n=10), experimental group Ⅱ (after induced DM and GEB dieted rats; n=10), experimental group Ⅲ (Prior GEB dieted thereafter DM induced; n=10). Prior and After GEB dieted application was that body weight, blood glucose were increase in experimental group Ⅱ, Ⅲ. Specially, group Ⅲ was significantly change than group Ⅱ at 1st weeks. and the level of CAT were significantly decrease in experimental group Ⅱ, Ⅲ than group Ⅰ. but SOD level was increase in experimental group Ⅱ, Ⅲ than group Ⅰ. In histological observation; group Ⅰ showed decrease in the intensity and incidence of vacuolations, cellular infiltration and hypertrophy of in liver and kidney. The Gomori's stain result, group Ⅰ showed disruption ${\beta}$-cell in pancrease.

• 대한한방내과학회지
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• 제29권4호
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• pp.1025-1036
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• 2008

Objectives : The aim of present study was to investigate recovery effects of gypsum, which has been used clinically in diabetes therapy. Methods : We established three groups, normal, control, and gypsum, and assigned 6 rats to each group. The normal group was not treated by any process and fed normal saline. The control & gypsum groups were administered streptozotocin(STZ) to induce diabetes. Gypsum extract was orally administered to the gypsum group for 10 days. After 8 weeks, the rats were sacrificed and their body weight, 24hrs urinary protein excretion, glucose, albumin, BUN, creatinine, total-cholesterol, LDL-cholesterol, triglyceride in blood, level of glycation end-product (AGE) and transforming growth factor ($TGF-{\beta}1$) in serum were measured. Morphological profiles and morphometric studies of the kidney cortex. renal transforming growth factor ($TGF-{\beta}1$) expression, macrophage/monocyte antigen (ED-1), and type IV collagen expression were studied. Results : The following results were obtained. The protein amount in urine per 24hrs of the gypsum-treated group as compared to the control was significantly reduced. The BUN and creatinine level in serum of the gypsum-treated group as compared to the control was significantly inhibited. The construction change in the kidneys of the gypsum-treated group as compared to the control was significantly inhibited. The factor of the gypsum-treated group as compared to the control was significantly inhibited. which induced the structural change in the kidneys. Conclusions : The above results suggest that gypsum partially improved kidney function.