• Journal of fish pathology
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• v.6 no.2
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• pp.93-101
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• 1993

The RNAI mutation of Saccharomyces cerevisia is a recessive and temperature sensitive lethal mutation which interferes with the production of mRNA, rRNA, and tRNA. However, the precise role of RNAI gene have not been revealed until yet. We have cloned rna1-1 mutant gene from rna1-1 mutant yeast strain(R49 ; trpl, ura3-52, rna1-1). The 3.4kb BglII fragment of wild type RNAI clone(81-2-6) contains whole RNAI gene. The genomic southern blotting with BglII digested R49 genomic DNA as a probe shows the unique and identical band with wild type 3.4kb BglII fragment. Therefore, We prepared partial BglII genomic library(3~4kb BglII fragments) into BamH I site of pUC19. The rna 1-1 mutant clone was screened with Digoxigenin(DIG)-lableled probe by high density colony hybridization. The 5'-flanking region of rna1-1 gene was sequenced by dideoxy chain termination method. The 5'-flanking sequence of RNAI gene contains three TATA-like sequence ; TAATA, TATA and TTTTAA at position of -67, -45, and -36 from first ATG codon respectively. The 5'-flanking region of wild type RNA I gene from ATG codon to -103nt was deleted with Bal31 exonuclease digestion, generating $pUC{\Delta}$/RNA I. After constructing $pYEP{\Delta}RNA$ I (consists of -103nt deleting RNA I gene, URA3 gene, $2{\mu}m$ rep. origin), pYEPrna1-1(consists of Xba I fragment of pUCrna1-1. URA3 gene, $2{\mu}m$ rep. origin), and pYEPRNAI. each plasmid was transformed into host strain(trpl, ura3-52, rna1-1) by electroporation, respectively. Yeast transformant carrying $pYEP{\Delta}RNA$ I did not complement the thermal sensitivity of rna1-1 gene. It means that TATA-like sequences in 5'-flanking region is not TATA sequence for transcribing RNAI gene and there may be other essential sequence in upstream region for the transcription of RNAI gene.

• Spatial Information Research
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• v.20 no.5
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• pp.99-109
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• 2012

Recently, due to the growth of social media and spread of smart-phone, the amount of data has considerably increased by full use of SNS (Social Network Service). According to it, the Big Data concept is come up and many researchers are seeking solutions to make the best use of big data. To maximize the creative value of the big data held by many companies, it is required to combine them with existing data. The physical and theoretical storage structures of data sources are so different that a system which can integrate and manage them is needed. In order to process big data, MapReduce is developed as a system which has advantages over processing data fast by distributed processing. However, it is difficult to construct and store a system for all key words. Due to the process of storage and search, it is to some extent difficult to do real-time processing. And it makes extra expenses to process complex event without structure of processing different data. In order to solve this problem, the existing Complex Event Processing System is supposed to be used. When it comes to complex event processing system, it gets data from different sources and combines them with each other to make it possible to do complex event processing that is useful for real-time processing specially in stream data. Nevertheless, unstructured data based on text of SNS and internet articles is managed as text type and there is a need to compare strings every time the query processing should be done. And it results in poor performance. Therefore, we try to make it possible to manage unstructured data and do query process fast in complex event processing system. And we extend the data complex function for giving theoretical schema of string. It is completed by changing the string key word into integer type with filtering which uses keyword set. In addition, by using the Complex Event Processing System and processing stream data at real-time of in-memory, we try to reduce the time of reading the query processing after it is stored in the disk.

• Parasites, Hosts and Diseases
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• v.21 no.2
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• pp.193-204
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• 1983

The Jinyang Lake is dammed up Nam River, and surrounded by Jinju city and four counties; Jinyang, Sanchong, Hading, and Sachon in kyongsang-Nam-Bo, Korea. The areas around this man-made lake have been known as an endemic focus of clonorchiasis in Korea. The present study was first aimed to know the distribution of freshwater mollusks including medically important snails, and larval trematodes shed from Parafossarulus manchouricus and Semisulcospira libertina. In addition to above studies, water analyses in each snail habitat were carried out in order to figure out a part of their environmental factors. This malaco-ecological survey was done at the sirs areas around upper, middle and lower parts of the lake for 4 months, August-November, 1983. Total nine species of freshwater mollusks were collected throughout the study: 4 species of gastropods: Semisulcospira libertina, Cipangopaludina chinensis, Parafossarulus manchouricus and Radin auricularia, and 5 species of bit.alves; Unio dcuglasiae, Ancdonta woodiana, Lamprotula gottschei, Corbicula yuminea and Limncperma lucustris. Out of nine species of freshwater mollusks, three species of gastropods; S. Zibertina, p. manchouricus and R. auricularia were medically important in terms of the transmission of digenetic trematodes to humans. P. manchouricus and R. auricularia were mainly collected from the shallow ponds and the irrigation channels with the muddy basin, but S. libertine and the bivalves were only collected from the stream of Nam River where the gravels and rocks were dominant. The levels of dissolved oxygen (D.O.) and biochemical oxygen demand \(B.O.D._5)\ of the water specimens sampled from the study areas ranged from 6.0 to 9.6 ppm and from 0.4 to 1.6 ppm respectively. As a result, it is considered that water system around the Jinyang Lake might be relatively clean without any heavy pollution of aquatic microorganisms and organic materials during the period of this study. On the other hand, eight metalic constituents from the water samples were also assayed, and all metalic ions detected were remarkably low below the legal criteria. However, calcium ion in the water samples from the habitats of P. manchouricus was considerably higher than others. Infection rates of digenetic trematodes in the snails were 6.9% in P. manchouricus and 4.8% in S. libertine, respectively. P. manchouricus snails harboring with thc cercariae of Clonorchis sinensis were only 0.14% among the snails examined, and other trematode cercariae except cercaria of C. sinensis were: furcocercus cercariae, cercaria of Loxogenes liberum type I and II. S. libertine snails parasitized with the cercariae of Metagonimus yokogawai were 1, 5% out of the snails examined, and no cercaria of Paragonimus westermani was found in S. libertine snails in the present study. Digenetic trematode cercariae other than M. yokogawai observed in S. libertina snails were: Cercaria yoshidae (B type) , Cercaria cristata, Cercaria innominatum, Cercaria of Centrocestus formosanus and Cercaria nipponensis.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.3
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• pp.31-41
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• 2016

Damage to structures, such as bridge piers, are increasing rapidly due to the debris moving along rivers at the time of flooding. Therefore, the debris fin, debris deflector and debris sweeper, which are debris reduction systems, were produced in this study and an accumulation experiment was carried out on the experimental channel according to the existence of the reduction system. The debris fin is the reduction system that creates parallel flow on debris accumulated on the bridge to pass through the bridge, which was produced using wood. In addition, the debris deflector was produced using steel pipes and it has the type of detouring the direction of debris. The debris sweeper passes the debris using the magnetic force rotation of a screw-shaped cylindrical structure by water flow and it was produced using acrylic material. The experiment was carried out by analyzing the level of accumulation according to the hardness and dropping method of the debris and comparing the accumulation rate of reduction systems, and the experiment was carried out 5 times. According to the experimental results, there was a difference in the accumulation rate according to the type of reduction system and the shape of debris, and it often depended significantly on the initial shape of debris accumulation. The direct debris reduction effect on the bridge was higher in the order of the debris deflector, debris sweeper and debris fin, but in case of the debris deflector, damage, such as stream turbulence, changes in water level and river bed, and the loss of deflector can occur due to debris accumulated directly on the debris deflector. Therefore, it is necessary to design the debris deflector considering these issues.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.2
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• pp.132-139
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• 2010

To manage the effluent nutrients amounts from rice paddy fields using free range ducks or rice bran, we evaluated the effects of a purifying paddy field which is no fertilizer, no pesticides, and dense rice seedling having a concept of constructed wetland. The experimental paddy field was located at downstream in the watershed of ducks using rice culture area in Milyang. The purifying paddy of land design were treated with seeding method, and vegetation type. As land design, direct seeding on plane, and direct seeding on high-ridge field in 2007. Planting rice only, and planting rice with water hyacinth were treated as vegetation type in purifying paddy in 2008. The purifying paddy fields were effective to reduce amount of T-N and T-P contents in effluent to 33.2~45.3%, and 53.1~55.4%, respectively. The direct seeding on high-ridge treatment, having long residence time of effluent water was more effective than plane plot as T-N 0.29 $g^{-1}m^{-2}d^{-1}$, and T-P 0.031 $g^{-1}m^{-2}d^{-1}$. The planting rice with water hyacinth treatment was effective than planting rice only as T-N 0.23 $g^{-1}m^{-2}d^{-1}$, and T-P 0.049 $g^{-1}m^{-2}d^{-1}$. The optimum area of purifying paddy field to treats all effluent were found out 3.2~4.7% of rice culture area using free range ducks, and rice bran at upper stream.

• Journal of the Korean Geographical Society
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• v.29 no.1
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• pp.3-15
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• 1994

The purpose of this study is to investigate the origin of traditional folkhouses of Japan in connection with those of Korea. Japanese folkhouses have received much influence from the Korean Peninsular, mainland China, northern Asia and Southeast Asia. The writer believes that the main stream of folkhouse culture origineted in the Korean Peninsular and flowed into the Japanese Islands. There ara many striking similarities in the folkhouses of Korea and Japan. Firstly, double-row room houses are distributed in the northeastern part of the Korean Penininsular and all parts of the Japanese Islands, and they are classified into the five-room type and four-room type. These types are very similar in both nations in the aspects of and floorplan and distributing patterns. Because floorplan may be used as an indicator of culture regions and cultural diffusion both, nations are believed to be closely related in the interchange of cultures in ancient times. Secondly, stables are allocated to the main house, and they are called "magu" in common "Magu" means horse stable, but there are no horses in it. In ancient times, however, many horses were kept in "magu" in both countries. Thirdly, there are much similarities in the function and structure of the folkhouses of both countries. The characteristics of folkhouses of Japan could be explained as the result of cultural diffusion from the Korean Peninsular.on from the Korean Peninsular.

• Korean Journal of Environmental Biology
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• v.37 no.2
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• pp.204-216
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• 2019

To evaluate the biological integrity of aquatic ecosystems, we developed Korean multimetric diatom index (KMDI) using metrics that many researchers have been previously described, and compared to single-metric diatom index such as trophic diatom index (TDI) and diatom assemblage index to organic pollution (DAIpo). For the biological and environmental data, we investigated the epilithic diatoms, water quality and the land-use for 923 sites in Korean streams between 2013-2017, and estuaries between 2010-2017, respectively. Five appropriate metrics were selected according to the following steps; 1) extraction of 300 potential metrics (biological, chemical, physical, and geographical) based on previous references, of these, 2) 46 samples having high separation power were selected, 3) the selected metrics were each tested for variability, redundancy, and sensitivity to the environments, finally 4) construction of multi-metric diatom index comprising single type metrics such as TDI, DAIpo, % motile diatoms, % Achnanthes / (Achnanthes+Navicula), and % number of Gomphonema species. The biological integrity of the 233 sites from the Geum River basin were independently investigated using KMDI. Collectively, the new KMDI showed high sensitivity and explanatory power for environmental factors such as land-use, biochemical oxygen demand, total nitrogen, and electric conductivity. However, it had slightly higher biological integrity for the same sites as compared to single type diatom metrics. Finally, more data accumulation from all over Korea and the development of acceptable diatom metrics were required.

• Journal of the Korean GEO-environmental Society
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• v.13 no.9
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• pp.31-44
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• 2012

Seasonal changes in groundwater geochemistry exist in Ddan-sum island. In the farming season of April, the spatial distribution of ions explains that $Fe^{2+}$, Fe(total), $Mn^{2+}$, $Ca^{2+}$, $Mg^{2+}$, $SiO_2$, ${HCO_3}^-$, and ${NO_3}^-$ are high in the center of the island, but $Na^+$ and $K^+$ are relatively low and these high anions indicates the effect of fertilizer used for strawberry cultivation. Spatial variation of ion concentration is smaller in August than April because of low agricultural activity and heavy rainfall. Geochemical type of groundwater shows that the center of island has the characteristics of recharge zone but the rim area corresponds to a mixing zone between groundwater and stream water. According to the analysis of saturation index for Fe and Mn ions, hematite, goethite, and rhodochrosite under supersaturation have a possibility of additional mineral deposition, and siderite, $Fe(OH)_3$, manganite, pyrolusite, and pyrochroite under unsaturation may exist as a type of dissolved ion.

• Journal of Korea Water Resources Association
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• v.40 no.12
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• pp.985-994
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• 2007

Small hydropower is the one of the cleanest one among new and renewable energy with less green house gas emissions. Recently, the necessity of developing small hydropower is emerging since its remaining potential resources in this country are abundant. However, a survey or research on the small hydropower development has been hardly performed since the 1990#s. These circumstances encourages a systematic approach for the small hydropower development. The purpose of this study is to propose a methodology of the location analysis for developing small hydropower. To this end, constraint and location criteria with weights are established and quantification method of each factor is presented. Especially, the analysis procedure is established on the basis of GSIS. Also the study focus on raising the objectivity and precision of analysis by developing system model with automatic search. The proposed methodology is applied to Bochung stream in Keum Riverbasin. The result selects the four and two locations of dam type and run-of-river type respectively. This study will be beneficial to the future activation of small hydropower development as a fundamental work.

• Journal of the Korean Association of Geographic Information Studies
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• v.10 no.4
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• pp.122-131
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• 2007

This study estimates how soil loss in a basin has been occurred according to the change of land cover, and analyzes which type of land cover has the largest soil loss by classifying the land-cover type into each area and a whole basin. Musimcheon, the second branch stream of GeumGang, is chosen as a research area. The result of analysis shows that the average soil loss occurs most largely in a crop land and a paddy field. The yearly soil loss of watershed estimates approximately 14,000 ton/yr in case of using 100-year-frequency rainfall data. A forest area, which takes the largest area in watershed, shows the soil loss occurs approximately 1,000ton/yr. A crop field shows that soil loss increased most largely 4,900 ton/yr (34.6%) in 1985 to 8,100 ton/yr (56.1%) in 2000. The change of land cover in a crop land increased 8% to 14%, and this change influences on the increase of soil loss. As a result of analyzing the area over $200ton/km^2/yr$, the soil loss in a crop field accounts for 74% to 96%.