• Journal of Embryo Transfer
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• v.28 no.3
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• pp.207-213
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• 2013

This study was carried out to examine a molecular marker system for parentage test in Jeju Black cattle (JBC). Based on the preliminarily studies, we finally selected for construction of a novel genetic marker system for molecular traceability, identity test, breed certification, and parentage test in JBC and its related industrial populations. The genetic marker system had eight MS markers, five indel markers, and two single nucleotide polymorphisms (SNPs; g.G299T and g.del310G) within MC1R gene which is critical to verify the breed specific genotypes for coat color of JBC differing from those of exotic black cattle breeds such as Holstein and Angus. The results showed lower level of a combined non-exclusion probability for second parent (NE-P2) of $4.1202{\times}10^{-4}$ than those previously recommended by International Society of Animal Genetics (ISAG) of $5.000{\times}10^{-4}$ for parentage, and a combined non-exclusion probability for sib identity (NE-SI) of $2.679{\times}10^{-5}$. Parentage analysis has been successfully identified the JBC offspring in the indigenous population and cattle farms used the certified AI semens for production using the JBC-derived offspring for commercial beef. This combined molecular marker system will be helpful to supply genetic information for parentage test and traceability and to develop the molecular breeding system for improvement of animal productivity in JBC population.

• Research in Plant Disease
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• v.10 no.2
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• pp.145-149
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• 2004

'Hongro' is one of the most important apple cultivars whose growing area is increasing because of its good quality. Recently fruit shrinking symptom causing decrease of fruit size, juice, and quality, appears in some commercial 'Hongro' orchards. The average frequency of occurrence of fruit shrinking symptom was 12 % of total trees investigated and Apple chlorotic leaf spot virus (ACLSV) was detected from all the trees showing fruit shrinking symptom by ELISA. A typical virus infection symptom of leaf epinasty and stem necrosis appeared on woody indicators, Spy227 and Virginia crab grafted with infected trees and all the grafted trees showed positive reaction to ACLSV antiserum by ELISA. It was proved that ACLSV can be easily transmitted by grafting. ACLSV was also isolated from the leaves of C. quinoa inoculated with sprouting leaf sap of infected trees. To prove that the fruit shrinking symptom was caused by ACLSV infection, ACLSV-infected scion was grafted on virus-free 'Hongro/M9' and the fruit characteristics were investigated. Consequently the same symptoms of fruit size and juice decreasing were observed from the trees grafted with ACLSV-infected scion. Therefore, it is suggested that the fruit shrinking symptom is caused by ACLSV infection and 'Hongro' can be classified as sensitive cultivar to ACLSV.

• Research in Plant Disease
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• v.12 no.3
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• pp.189-196
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• 2006

Crown gall on lower stem of weeping fig(Ficus benjamina Roxb.) was first observed at Daejon in 2003. Tumors were about 15 cm in size and semi-round with rough surface texture of dark brown color. Two virulent isolates among ten bacteria isolated from the tumor tissues were characterized. Their colonies were convex, glistening, circular with an entire edge, and white or tannish cream in color on potato dextrose agar supplemented with 0.5% $CaCO_3$. They were rod shape with peritrichous flagellae, gram-negative, aerobic growth, oxidase-positive, and grew on D1M agar. The isolates were identified as Agrobacterium larrymoorei and A. tumefaciens based on biochemical and physiological characteristics, fatty acid profiles and substrate utilization patterns. Seedlings of some host plants excepting grapevine produced typical galls two to three weeks after inoculation with cell suspensions of the virulent strains. This is the first report on crown gall of weeping fig in Korea.

• Journal of The Korean Association For Science Education
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• v.32 no.2
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• pp.320-330
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• 2012

Understanding the relation between basic science and applied science is important for students in understanding the importance of learning science, the relationship between scientific knowledge and human life, and for enhancing their science motivation. In this study, we evaluated the statistical validity of this hypothetical model and explored the effect of gender and students' preferred courses (e.g., humanities, science, and art) on four dependent variables. We also evaluated the differences of students' understanding across scientific domains and students' understanding concerning basic and applied scientific knowledge. Three hundred and twenty five 10th grade students participated in this survey research. Statistically, we employed bivariate correlation, partial correlation, path analysis, two-way ANOVA, and repeated measures ANOVA. Our findings illustrated that our hypothetical model was statistically valid. In addition, the significant interaction effects of gender and students' preferred courses on each dependent variable were shown. Students have different levels of understanding of the convergence of basic and applied science, the relation between scientific knowledge and human life, and the importance of learning science across scientific domains (e.g., physics, chemistry, earth science and biology).

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.4
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• pp.488-500
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• 2013

The objective of this study was to compare the effectiveness of the protocols for superstimulation of follicular growth in Thai native heifers. Heifers (n = 20) were randomly divided into four groups of five heifers/group. Heifers were given a single dose by i.m. administration of 100 mg Follicle Stimulating Hormone dissolved in polyvinylpyrrolidone (FSHp) at 24 h. Ovum pick up (OPU) occurred at 72 h ($F_{24}O_{72}$ protocol; Group 1) or 96 h ($F_{24}O_{96}$ protocol; Group 2), and at 36 h and OPU at 72 h ($F_{36}O_{72}$ protocol; Group 3) or 96 h ($F_{36}O_{96}$ protocol; Group 4) after follicular ablation. The dynamics of ovarian follicular growth were monitored by twice-daily ultrasonographic examinations. Blood sample collections were performed every 12 h after initiation of treatment for assessment of FSH, E2 and P4 profiles. All heifers were subjected to eight repeated sequential sessions of OPU. The follicular deviation commenced $24{\pm}5.32$ h after follicular ablation in all groups. The circulatory FSH surged quickly from 24 to 36 h (>0.8 ng/ml) after follicular ablation and circulatory estrogen levels steadily increased from 36 h until OPU in all groups. At the end of the OPU sessions, the mean number of aspirated follicles/heifer/session in $F_{36}O_{72}$ protocol (Group 3) and $F_{36}O_{96}$ protocol (Group 4) were higher than in the two other groups (p<0.05). The number of cumulus-oocyte complexes (COCs), cleaved and day 8 blastocysts rates in the $F_{36}O_{72}$ protocol (Group 3) were higher than in the other groups (p<0.05). It can be concluded that a single dose i.m. administration of 100 mg FSHp at 36 h and OPU at 72 h after follicular ablation ($F_{36}O_{72}$ protocol; Group 3) was the most effective protocol for superstimulation of follicular growth for repeated OPU and subsequent in vitro embryo production in Thai native heifers.

• Journal of Korean Library and Information Science Society
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• v.28
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• pp.447-475
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• 1998

We are seeing a shift of the focus of information service from system-centered(data-centered or intermediary-centered) a n.0, pproach to user-centered a n.0, pproach. Sense-making theory is one of the user- centered a n.0, pproaches. This study focused on user-centered information service paradigm concepts of Dervin's sense-making theory and interpreting sense-making theory in the practical context of library services. The sense-making theory is basically a cognitive a n.0, pproach to information-seeking, in that it recognizes information as something that involves internal cognitive processes. The user of information becomes the focus in this sense-making model. The sense-making theory sees information as subjective, situational, and cognitive. It focuses on understanding information within specific contexts and on understanding how information needs develop and how they are satisfied. It regards the user not as a passive receive of external information but as the center in a active, ongoing process of change. It related to the information needs of users. Dervin's method for studying information needs employs the 'situation-gap-use' metaphor. All information needs stem from a discontinuity or 'gap! in one's knowledge. Dervin believes that information needs can be addressed by understanding the process that each individual goes through in experiencing a gap, in trying to resolve it, and in gaining something (especially new knowledge) from the experience. Sense-making is a process; 'sense' is the product of this process. Sense includes 'knowledge'. However, it includes a host of other subjective factors that reflect an individual's interpretations of a situation including intuitions, opinions, hunches, effective responses, evaluations, questions, etc. For several reasons, however, difficulties are encountered if one attempts a more substantial assessment of the theory. First, it has not yet crystallized into a well-defined theory. Secondly, though the theory has aroused general interest, e.g. within LIS, detailed discussion of its strong and weak points is still lacking. A third source of difficulty is that the basic assumptions of the theory have been explicated metaphorically. If sense-making theory is to be really useful, it needs to be interpreted for library settings. But we have found no attempt to construct an information service based on the theory. Because the research tradition is still relatively young, there are many unanswered questions connected, e.g. with the design of information systems. In any case, e.g. intermediary access systems would be more efficient and effective it their planning could be founded on the user-centered a n.0, pproach.

• Journal of Korean Society of Forest Science
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• v.77 no.2
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• pp.186-192
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• 1988

This study was carried out to estimate the stand form factor that is necessary to estimate stand volume by STRAND method among plotless sampling methods. The data measured for this study were based on the 380 sample plots from Larix leptolepis and 358 sample plots from Pinus koraiensis which were distributed in the region of Kyeongi, Kangweon, Chungbuk, Chungnam, Chun-buk and Kyeongbuk. 1. Stand form factor was highly correlated with in the following order, stand form height, stand average height, average diameter, stand age, distance of stem, and basal area height. 2. The best fitted equation of stand form factor of above two tree species were presented in table 3. 3. Stand form factor tables using estimated equations on the table 3 were prepared and presented in table 4, 5(Larix leptolepis), 6, 7(Pinus karaiensis). 4. The relatioinships between estimated value and actual value were Y=bx, where b approached nearly 1.0, and there were not any significant differences between them. 5. The percentages of estimated error on stand form factor table were ranged from 2.39% to 4.15% in Larix leptolepis and from 1.73% to 2.50% in Pinus koraiensis. Therefore, the stand form factor could be exactly estimated by use of these tables.

• Journal of Korean Society of Forest Science
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• v.89 no.1
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• pp.116-125
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• 2000

This study was conducted to investigate the effects of CEPA(2-chloroethyl phosphonic acid) on bark physiology and anatomy of lactree(Rhus verniciflua Stokes). Sample trees of similar size and growth rate were selected from 7-year-old lactree plantation located in Hyengsung-kun, Kangwon-do. Lanolin pastes containing 0.1, 1, or 10% CEPA were put into the bark-removed hole made by corer(${\phi}1cm$) on the main stem at 1.2m above the ground on June 16, 1995. Five weeks after application of CEPA, bark thickness was markedly increased as a result of the increase in the amount of phloem and intercellular spaces, and correlated with the increased production of urushiol. By the application of 10% CEPA, bark thickness was increased approximately 2.5 times, and the urushiol content within bark was increased 2.8 times compared to that of untreated trees because CEPA stimulated the accumulation of urushiol within bark. Treatment of 10% CEPA also increased the size and the total number of secretory canals, and induced an increase in ray width. The phloem parenchyma cells of CEPA-treated trees were well-developed and closely packed with little intercellular space.

• Journal of Bio-Environment Control
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• v.10 no.4
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• pp.251-257
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• 2001

This experiment was conducted to investigate the effect of chitosan on the growth and yield of fall-planted potatoes. The research was carried out in fall-planted potatoes from August 2000 to Aprial 2001 at the height of 250m above sea level in Jeju Island. Soaking for 30 min. of seed potatoes in 200 times diluted solution of 3% liquid chitosan shortened days to emergence by 4 to 6 days. Stem length, number of stems and number of stolons per plant were 56.5 cm, 4.3, and 19.0, respectively, when seed potatoes were soaked for 30 min. in 200 times diluted solution of 3% liquid chitossan. Total potato yield in this treatment reached 2,963 kg per 10a and was significantly greater than in other treatments. Yield of marketable tubers (greater than 30g) per 10a for treatment of seed potatoes soaking in 200 times diluted solution of 3% liquid chitosan and for treatment of foliage spray with 200 times diluted solution of 3% liquid chitosan plus chitosna power (10 kg per 10a) miximg with soil was 2,761 kg and 2,628 kg, respectively. Contents of Mg, Fe and B were the greatest in the treatment of 30 min. soaking of seed potatoes in 200 times diluted solution of 3% liquid chitosanm. The increased contents of these elements are considered to have caused yield increase as a result of increased chlorophyll content for photosynthesis.

• Journal of Wetlands Research
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• v.14 no.4
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• pp.597-605
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• 2012

This study was conducted in order to determine changes in the growth responses of Quercus serrata and Q. aliena which are potential natural vegetation of riverine in Korea under four light gradients within ambient and elevated $CO_2$ concentration and temperature levels. As a result, growth responses of two species were affected by light factor. Aboveground, belowground, plant biomass and root:shoot ratio of two species grown under the control and treatment were increased in the highest light level. Plant biomass and root:shoot ratio of two oak species were not significantly affected by elevated $CO_2$ and temperature, while aboveground biomass of them was lower in the treatment than control. Belowground biomass of Q. serrata was lower in the treatment than control under the gradients that are more than 70% of light level. As light intensity increases, elevated $CO_2$ and temperature promoted root growth of two oak species but had a negative effect on aboveground growth. According to the principal component analysis(PCA), two oak species were discriminatively arranged based on factor 1 and 2. Also, the reactions towards the ambient and elevated $CO_2$ and temperature were slightly different. It is clearly visible that all features relied on axis 1 and axis II are highly correlated with most variables except for stem and shoot length.