Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Crown Gall of Weeping Fig Caused by Agrobacterium larrymoorei and A. tumefaciens

Agrobacterium larrymoorei와 A. tumefaciens에 의한 벤자민고무나무 뿌리혹병

  • Lee, Young-Kee (Plant Pathology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Hwang, Hye-Kyung (Plant Pathology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Hwang, Tae-Ho (Plant Pathology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Myung, Inn-Shik (Plant Pathology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Koo, Han-Mo (Department of Plant Resources, College of Industrial Science, Kongju National University) ;
  • Cha, Jae-Soon (Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
  • 이영기 (농업과학기술원 농업생물부 식물병리과) ;
  • 황혜경 (농업과학기술원 농업생물부 식물병리과) ;
  • 황태호 (농업과학기술원 농업생물부 식물병리과) ;
  • 명인식 (농업과학기술원 농업생물부 식물병리과) ;
  • 구한모 (공주대학교 산업과학대학 식물자원과) ;
  • 차재순 (충북대학교 농업생명환경대학 식물의학과)
  • Published : 2006.12.01
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Abstract

Crown gall on lower stem of weeping fig(Ficus benjamina Roxb.) was first observed at Daejon in 2003. Tumors were about 15 cm in size and semi-round with rough surface texture of dark brown color. Two virulent isolates among ten bacteria isolated from the tumor tissues were characterized. Their colonies were convex, glistening, circular with an entire edge, and white or tannish cream in color on potato dextrose agar supplemented with 0.5% $CaCO_3$. They were rod shape with peritrichous flagellae, gram-negative, aerobic growth, oxidase-positive, and grew on D1M agar. The isolates were identified as Agrobacterium larrymoorei and A. tumefaciens based on biochemical and physiological characteristics, fatty acid profiles and substrate utilization patterns. Seedlings of some host plants excepting grapevine produced typical galls two to three weeks after inoculation with cell suspensions of the virulent strains. This is the first report on crown gall of weeping fig in Korea.

2003년 대전광역시 유성구에서 벤자민고무나무의 줄기에 뿌리혹병이 발생하였다. 혹은 15cm정도의 크기로 표면이 거칠고 갈변된 타원형이었다. 혹 조직으로부터 Agrobacterium과 유사한 세균을 분리하였으며 벤자민고무나무의 유묘에 접종하여 혹을 형성한 5개 균주 중에서 2가지 계통에 대하여 분류학적 특성을 조사하였다. 0.5% $CaCO_3$가 첨가된 PDA 배지에서 생장된 병원성 세균들은 둥글고 볼록하면서 광택이 나는 크림색 계통이었다. 모든 균주는 다수의 편모를 가진 간상의 세균으로 그람음성이었으며 호기적으로 생장하면서 D1M agar에서도 생장하였다. 병원성 세균들은 대조균주와 상이한 특성을 가지고 있었으나 주요 생리 생화학적특성, 탄소원 이용양상, 지방산조성 분석 결과에 근거하여 A. larrymoorei와 A. tumefacien로 동정되었다. A. larrymoorei와 A. tumefaciens는 포도를 제외한 타 기주식물에서도 병원성이 있었다. 국내에서 A. larrymoorei와 A. tumefaciens에 의한 벤자민고무나무의 뿌리혹병은 본 연구에서 처음 보고된다.

Keywords

References

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