Browse > Article
http://dx.doi.org/10.5423/RPD.2006.12.3.189

Crown Gall of Weeping Fig Caused by Agrobacterium larrymoorei and A. tumefaciens  

Lee, Young-Kee (Plant Pathology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Hwang, Hye-Kyung (Plant Pathology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Hwang, Tae-Ho (Plant Pathology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Myung, Inn-Shik (Plant Pathology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Koo, Han-Mo (Department of Plant Resources, College of Industrial Science, Kongju National University)
Cha, Jae-Soon (Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
Publication Information
Research in Plant Disease / v.12, no.3, 2006 , pp. 189-196 More about this Journal
Abstract
Crown gall on lower stem of weeping fig(Ficus benjamina Roxb.) was first observed at Daejon in 2003. Tumors were about 15 cm in size and semi-round with rough surface texture of dark brown color. Two virulent isolates among ten bacteria isolated from the tumor tissues were characterized. Their colonies were convex, glistening, circular with an entire edge, and white or tannish cream in color on potato dextrose agar supplemented with 0.5% $CaCO_3$. They were rod shape with peritrichous flagellae, gram-negative, aerobic growth, oxidase-positive, and grew on D1M agar. The isolates were identified as Agrobacterium larrymoorei and A. tumefaciens based on biochemical and physiological characteristics, fatty acid profiles and substrate utilization patterns. Seedlings of some host plants excepting grapevine produced typical galls two to three weeks after inoculation with cell suspensions of the virulent strains. This is the first report on crown gall of weeping fig in Korea.
Keywords
Agrobacterium larrymoorei; A. tumefaciens; Crown gall; Ficus benjamina; Weeping fig;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Miller, L. T. 1982. Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J. Clin. Microbiol. 16: 584-586
2 Sawada, H., Ieki, H., Oyaizu, H. and Matsumoto, S. 1993. Proposal for rejection of Agrobacterium tumefaciens and revised descriptions for the genus Agrobacterium and for Agrobacterium radiobacter and Agrobacterium rhizogenes. Int. J. Syst. Bacteriol. 43: 694-702   DOI   ScienceOn
3 윤평섭. 1989. 한국원예식물도감. 지식산업사. pp. 658-659
4 Bouzar, H. and Jones, J. B. 1992. Distinction of biovar 2 strains of Agrobacterium from other chromosomal groups by differential acid production. Lett. Appl. Microbiol. 15: 83-85   DOI
5 DeCleene, M. and De Ley, J. 1976. The host range of crown gall. Bot. Rev. 41: 389-466
6 Bouzar, H. and Jones, J. B. 2001. Agrobacterium larrymoorei sp. nov., a pathogen isolated from aerial tumours of Ficus benjamina. Int. J. Syst. Evol. Microbiol. 51: 1023-1026   DOI   ScienceOn
7 Perry, K. L. and Kado, C. I. 1982. Characteristics of Ti plasmids from broad-host-range and ecologically specific biotype 2 and 3 strains for Agrobacterium tumefaciens. J. Bacteriol. 151: 343-350
8 Zoina, A., Raio, A., Peluso, R. and Spasiano, A. 2001. Characterization of agrobacteria from weeping fig (Ficus benjamina). Plant Pathology 50: 620-627   DOI   ScienceOn
9 Bouzar, H., Chilton, W. S., Nesme, X., Dessaux, Y., Vaudequin, V., Petit, A., Jones, J. B. and Hodge, N. C. 1995. A new Agrobacterium strain isolated from aerial tumors on Ficus benjamina L. Appl. Environ. Microbiol. 61: 65-73
10 Young, J. M., Kuykendall, L. D., Martinez-Romero, E., Kerr, A. and Sawada, H. 2001. A revision of Rhizobium Frank 1889, with an emended description of the genus, and the inclusion of all species of Agrobacterium Conn 1942 and Allorhizobium undicola de Lajudie et al. 1998 as new combinations: Rhizobium radiobacter, R. rhizogenes, R. rubi, R. undicola and R. vitis. Int. J. Syst. Evol. Microbiol. 51: 89-103   DOI
11 Schaad, N. W., Jones, J. B. and Chun, W. 2001. Laboratory guide for identification of plant pathogenic bacteria, 3rd ed. APS Press, St. Paul, Minn. 373 pp
12 Chase, A. R. 1987. Compendium of ornamental foliage plant diseases. APS Press, St. Paul, Minn
13 Galloway, B. T. 1919. Giant crown galls from the Florida Everglades. Phytopathology 9: 207-208
14 Bouzar, H., Jones, J. B. and Hodge, N. C. 1993. Differential characterization of Agrobacterium species using carbonsource utilization patterns and fatty acid profiles. Phytopathology 83: 733-739   DOI
15 Kersters, K. and De Ley, J. 1984. Genus Agrobacterium. In Bergey's manual of systematic bacteriology, Vol. 1. by Krieg, N. R. and Holt, J. G., eds., pp. 244-254. The Williams and Wilkins, Baltimore