Objective: Hyperstimulation methods are broadly used for in vitro fertilization (IVF) in patients with infertility; however, the side effects associated with these therapies, such as ovarian hyperstimulation syndrome (OHSS), have not been well studied. N-glycoproteomes are subproteomes used for the remote sensing of ovarian stimulation in follicular growth. Glycoproteomic variation in human follicular fluid (hFF) has not been evaluated. In this study, we aimed to identify and quantify the glycoproteomes and N-glycoproteins (N-GPs) in natural and stimulated hFF using label-free nano-liquid chromatography/electrospray ionization-quad time-of-flight mass spectrometry. Methods: For profiling of the total proteome and glycoproteome, pooled protein samples from natural and stimulated hFF samples were selectively isolated using hydrazide chemistry to obtain the total proteomes and glycoproteomes. N-GPs were validated by the consensus sequence N-X-S/T (92.2% specificity for the N-glycomotif at p<0.05). All data were compared between natural versus hyperstimulated hFF samples. Results: We detected 41 and 44 N-GPs in the natural and stimulated hFF samples, respectively. Importantly, we identified 11 N-GPs with greater than two-fold upregulation in stimulated hFF samples compared to natural hFF samples. We also validated the novel N-GPs thyroxine-binding globulin, vitamin D-binding protein, and complement proteins C3 and C9. Conclusion: We identified and classified N-GPs in hFF to improve our understanding of follicular physiology in patients requiring assisted reproduction. Our results provided important insights into the prevention of hyperstimulation side effects, such as OHSS.
Journal of The Korean Society of Grassland and Forage Science
/
v.19
no.1
/
pp.63-74
/
1999
This experiment was carried out to determine voluntary intake according to growth stage of sorghum $\times$ sudangrass hybrid in mono and intercropping. The results obtained from these experiments are as follows; Crude protein content of intercropping cultivation(sorghum $\times$ sudangrass hybrid + soybean) was higher than monocropping(sorghum $\times$ sudangrass hybrid), while ADF content was not different. Intake rates of leaf and stem showed highly at the intercropping comparing with monocropping(180cm~milk stage), but there was no difference at dough and yellow stage. Also, voluntary intake of fresh and dry matter showed same tendency. Voluntary intake of dry matter was the highest value of $98.4g/kg^{0.75}$ at milk stage treatment of intercropping. The intakes of crude protein were 9.6, 8.5, 7.2 and $7.2g/kg^{0.75}$ at 180cm, 250cm, boot stage and milk stage of intercropping treatment, respectively. These were higher than those for monocropping treatment. As mentioned above the results, intercropping cultivation could be recommended as increasing method of sorghum $\times$ sudangrass hybrid utilization when intake of dry matter and crude protein were considered.
Journal of The Korean Association For Science Education
/
v.34
no.3
/
pp.321-330
/
2014
Changes are expected in the future, and the future society will expect changes in education. Science curriculum needs to reflect such demands for changes in the future of education. Hence, this study explored ways to reflect the changes demanded by the future society in science education. In this study, we investigated the major issues and directions for improvements based on the findings from questionnaires given to 447 primary and secondary school science teachers as well as in-depth interviews with 12 experts. We explored the problems of the 2009 revised national science curriculum including organization of science elective courses, fusion 'science' as an elective course, intensive course-taking of science, career-focused science curriculum, variation of completion units in science elective courses, and fairness of science elective course selection in college entrance. In addition, we proposed ways to organize science curriculum around core competencies and STEAM education suggested by science teachers. According to the results, we need to add such key competencies as basic learning abilities, self-identity, and moral competencies to science curriculum in addition to existing key competencies including problem solving and communication. Regarding the fusion science, experts contended that convergence of science courses should come before that of science and other subjects, and that STEAM with science as the axis was the desired form of convergence. We also need to establish a curriculum development center that exclusively focuses on science curriculum research and development.
Cho, Yun-Hee;Kim, Chi-Seon;Kim, Jeong-Man;Ku, Yang Gyu;Kim, Ho Cheol
Horticultural Science & Technology
/
v.34
no.5
/
pp.719-726
/
2016
The effect of high and low temperatures on seedling quality while raising of paprika (Capsicum annuum L.) seedlings, and their early development after planting was investigated. The control raising seedling temperature (RST) was $23^{\circ}C$; high temperature, $31^{\circ}C$; and low temperature, $15^{\circ}C$ throughout the raising seedling period. At $15^{\circ}C$ and $30^{\circ}C$, plant height, stem diameter, fresh weight, dry weight, number of leaves, and seedling leaf area were significantly lower than those at $23^{\circ}C$. At 4 weeks after planting, seedling's growth characteristics showed a similar pattern. Compared to $23^{\circ}C$, seedlings raised at $15^{\circ}C$ and $30^{\circ}C$ had an increased dry weight and leaf area per unit time after planting than during the seedling raising period. At 4 weeks after planting, crop growth rate and leaf area index were unaffected by RST, and relative growth rate and net assimilation rate at RSTs of $15^{\circ}C$ and $31^{\circ}C$ were higher than those those at RST of $23^{\circ}C$. At an RST of $15^{\circ}C$, growth speed and net assimilation rates were higher after planting than before planting, according to increased photosynthetic rate. Thus, high and low temperatures during the seedling raising period significantly reduced seedling growth and plant growth after planting. After planting, seedlings raised at $15^{\circ}C$ recovered more quickly than did those raised at $31^{\circ}C$.
Journal of The Korean Society of Grassland and Forage Science
/
v.30
no.1
/
pp.1-8
/
2010
Few management studies have been conducted on late planting date for corn silage in paddy field as a barley-corn forage cropping system. Experiments were conducted during 2007 and 2008 at the Chungnam Agricultural Research & Extension Services. The objectives of this study were to determine relationships between planting date and com forage yield and to determine the best hybrid at the delayed planting after whole crop barley's harvest. The treatments consisted of 2 planting dates and 7 hybrids. Delayed planting considerately reduced stem diameter, individual ear size and weight. The ratio of dried leaf and culm in aerial plants was increased and that of ear was decreased. The coefficient of variation in fresh, dry matter (DM) and total digestible nutrients (TDN) yield was higher at delayed planting date on cropping system with whole crop barley than that of planting at optimum season. So, fresh weight was reduced by 91.8%, dry matter by 72.6%, grain yield by 51.0% and TDN yield by 68.1%. The appropriate hybrid for delayed planting after whole crop barley harvest in middle region was 'Gangdaok' as lower in the reduction of ear size and weight compared to that of planting at optimum season. Reduction in grain yield caused to decrease the DM and TDN yields. Therefore, to gain stable dry matter in silage corn by delayed planting date on cropping system with whole crop barley was necessary to select com hybrid on the minimum reduction in ear size and weight.
Bacterial pustule of soybean (Glycine max) caused by Xanthomonas axonopodis pv. glycines is one of the most prevalent bacterial diseases of soybean in Korea, where it causes considerable yield loss. This study was carried out to develop yield prediction model for bacterial pustule by analyzing correlation between the percentage of diseased leaf area and yield. The severe disease incidence of soybean bacterial pustule caused yield losses by 19.8% in 2006 and 16.8% in 2007, respectively. Severity of bacterial pustule greatly affected on 100 seed weight and yield, but did not on stem length, number of branches per plant, number of pods per plant, number of seeds per plant. On the other hand, correlation coefficients between diseased leaf area and yield were $-0.93^*$('06) and $-0.77^*$('07), respectively. The regression equation obtained by analyzing correlation between the percentage of diseased leaf area and yield loss in 2006 and in 2007 was y = -3.2914x + 348.19($R^2$ = 0.8603) and y = -2.9671x + 302.08($R^2$ = 0.9411), respectively. These results will be helpful in estimating losses on a field-scale and thereby predicting the production of soybean.
Melon necrotic spot virus (MNSV) is a very destructive disease to melon (Cucumis melo) plants. A MNSV was isolated from melon leaf showing necrotic spot symptoms at the plastic house in Naju, Korea in 2009. The isolate, designated as MNSV-Me, was identified and characterized by biological responses on several host plants, immuno captured RT-PCR and partial nucleotide sequencings of the genome. To evaluate MNSV-Me resistance in melon, thirty-five melon cultivars were mechanically inoculated on the cotyledon of the seedlings with the virus. MNSV-Me produced necrotic spots on the inoculated leaves of the all melon cultivars tested. Twenty-five cultivars were susceptible to the virus and they showed systemic necrotic spots on the leaves and/or necrosis longer than 3 cm in length on the stems within about forty days after inoculation. Five cultivars gave moderate resistance, no symptoms on the upper leaves but necrosis on the stem shorter than 3 cm in length. In an evaluation of MNSV-Me resistance in melon cultivars, 'Elstitan', 'Elsluxery', 'Betalichihage', 'Betalichi' and 'Womderfulhagae 1st' were found to have resistance by showing only faint necrosis on their stems.
The experiment was carried out to define the effect of the boron in nutrient solution on the development of mulberry root and cold damage. The length of shoots only attained about 50cm and then stopped in case of boron deficiency, while the mulberry trees developed vigorous and attained over 190cm long under boron supply condition. When boron is deficient, the measles appeared on surface of the stem and the necrosis appeared on the petiole, midribs and veins of leaves. At 70 days after planting, almost all new roots and old roots changed to brown, the lateral roots became necrosis by boron deficiency. After three days of stopping boron supply, many new roots changed to brown and after two days of boron resupply, new roots began to grow out and then new roots normally developed. Content of boron in leaves and barks increased by the increase of boron concentration in nutrient solution and maximum boron content in leaves and barks was obtained with boron-sufficient treatment of 0.5ppm. Treatment of 0.5ppm boron supply indicated that the leaves and barks contained more phospholipid, protein, sugar, RNA and proline than treatment of 0.01ppm boron supply in nutrient solution. The cuttings grown in boron supply nutrition have a sufficient tolerance at -10$^{\circ}C$ and -15$^{\circ}C$ for 24 hours while cuttings grown in boron-deficient nutrition have a weak tolerance at the same condition. As mentioned above, we can conclude that the effect of boron deficiency on root development is much severe and the relationship between the lack of boron and cold damage is very closely related.
Park, Hye-Yoon;Kim, Su-Na;Kang, Byung-Ha;Lee, John-Hwan
Korean Journal of Oriental Medicine
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v.16
no.3
/
pp.167-173
/
2010
Objects : Glycine max Merr. (black bean), Triticum aestivum L. (wheat) and Oryza sativa L. (rice bran) have been widely used for treatment of relaxion of smooth muscle, gastrointestinal hemorrhage and alopecia in Korean Traditional Medicine. In this research, we examined the effect of the extracts, obtained from EtOH extracts of 3 kinds of traditional plants, on hair growing activity of the DP6 and C3H10T1 cell and physical properties. Materials and Methods : On the basis of previous studies, three traditional plants were selected and we extracted them with ethanol. We evaluated their hairy dermal papillar cell proliferation activity and mouse mesenchymal stem cell in vitro model. Also, 3 herbal extracts were added to the normal shampoo formulation in ranges of 0.1% and we validated tensile properties and physical changes using aged hair. In this research, we compared the tensile strength, shine and color appearance between the hair (general formulation) and the hair after applying shampoo with natural extracts. To analyze the luster and color image, we use the SAMBA hardware and software made by Bossa Nova Technologies. Results : In the comparative test for tensile characteristic between the hair treated general formulation(control) and the hair applying special formulation including 3 kinds of extracts, tensile distance and energy of the latter are larger than control on average. The shine and color appearance were also increased after using shampoo including natural extracts(shine : 10.9%, color appearance: 24.12%). We observed the enhancement of hair growth activity in the DP6 and C3H10T1 cell. Especially black bean extracts had the most powerful effect in the dermal papillar cell proliferation. Conclusion : These experiments suggest that extracts of Glycine max Merr. (black bean), Triticum aestivum L. (wheat) and Oryza sativa L. (rice bran) stimulate the hair growth activity and can improve physical activities of aged hair. Shampoo product, which contains 3 kinds of natural extracts, would be used for the treatment for aged hair.
This study was conducted to investigate the effect of fine bubble water treatment on the growth of ginseng seedlings. The growth of ginseng seedlings which were treated with general water and fine bubble water was investigated. The above ground part, the growth of leaf was increased by about 10% and the growth of the stem was about 6%, as the ginseng seedlings which were treated with fine bubble water comparing to the ginseng seedlings treated with general water. Root length was increased about 5%, root width was increased about 8%, roots weight was increased about 9%, and dry weight was increased about 7%. This is not because the whole root growth was increased, but the main root growth was increased about 7%. These results suggested that the physical properties of the fine bubble water stimulated the growth of ginseng seedlings. These results are initial study in the case of ginseng seedlings. Therefore, it can be applicable to the 3-5 years old ginseng plants. Further research will be needed to find out the optimal cultivation condition by controlling the dissolved oxygen amount according to the soil condition and the research applied to the ginseng seedlings.
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