• Journal of Veterinary Science
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• v.23 no.4
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• pp.60.1-60.6
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• 2022

Background: A definite diagnosis should be made in the bovine practice field, however, it was difficult to perform laboratory analysis immediately. Currently, three types of portable blood gas analyzers are available in Korea. Objectives: This study aimed to evaluate the correlations among these three analyzers. Methods: Seventy-two plasma samples from Holstein-Friesian cows were used for blood gas analysis, and three instruments (EDAN i15 Vet, VETSCAN i-STAT, and EPOC) were operated simultaneously. Moreover, plasma calcium levels were compared between these portable analyzers and blood chemistry device, which is usually used in a laboratory environment. Pearson analysis was performed to confirm the correlation of each parameter produced with the three instruments and blood chemistry analyzer. Results: As results, high correlation was observed in parameters of pH, pO₂, potassium ion, ionized calcium, and glucose (p < 0.001, r > 0.7). In addition, pCO₂ showed a moderate correlation among the three analyzers (p < 0.001, r > 0.5), and there was no correlation among all instruments for sodium ions. There was also a high correlation between ionized calcium from the three portable devices and total calcium from the biochemistry analyzer (p < 0.001, r > 0.9). Conclusions: In conclusion, there was a high correlation between results from the three different blood gas analyzers used in the bovine clinical field in Korea. Thus, a consistent diagnosis can be made even with different equipment if the operator is aware of the strengths and weaknesses of each piece of equipment and operates it properly.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.35 no.2
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• pp.1-12
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• 2022

Objectives : This study was conducted to verify the anti-inflammatory effects of Aster glehni water extracts in HaCaT keratinocytes. Methods : In this study, cell viability was confirmed by MTT assay. Production of TNF-α and IL-6 was determined by ELISA. mRNA expression of TARC and MDC were measusred by qRT-PCR. Also, expressions of p-JNK, JNK, p-ERK, ERK, p-p38, and p-38 were investigated by using western blot assay. Results : Aster glehni water extracts were not shown any significant cytotoxicity at 15.625-500㎍/㎖ in HaCaT keratinocytes. Aster glehni extracts inhibited the TNF-α and IL-6 production in HaCaT keratinocytes treated with TNF-α and IFN-γ. Also, expression of TARC, MDC, p-ERK, and p-STAT1 was decreased. Conclusions : These results suggest that Aster glehni water extracts have anti-inflammatory effects in HaCaT keratinocytes and can be applied to the development of anti-inflammatory treatment substances.

• Journal of Ginseng Research
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• v.47 no.2
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• pp.183-192
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• 2023

Viral infections are known as one of the major factors causing death. Ginseng is a medicinal plant that demonstrated a wide range of antiviral potential, and saponins are the major bioactive ingredients in the genus Panax with vast therapeutic potential. Studies focusing on the antiviral activity of the genus Panax plant-derived agents (extracts and saponins) and their mechanisms were identified and summarized, including contributions mainly from January 2016 until January 2022. P. ginseng, P. notoginseng, and P. quinquefolius were included in the review as valuable medicinal herbs against infections with 14 types of viruses. Reports from 9 extracts and 12 bioactive saponins were included, with 6 types of protopanaxadiol (PPD) ginsenosides and 6 types of protopanaxatriol (PPT) ginsenosides. The mechanisms mainly involved the inhibition of viral attachment and replication, the modulation of immune response by regulating signaling pathways, including the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway, cystathionine γ-lyase (CSE)/hydrogen sulfide (H₂S) pathway, phosphoinositide-dependent kinase-1 (PDK1)/ protein kinase B (Akt) signaling pathway, c-Jun N-terminal kinase (JNK)/activator protein-1 (AP-1) pathway, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway. This review includes detailed information about the mentioned antiviral effects of the genus Panax extracts and saponins in vitro and in vivo, and in human clinical trials, which provides a scientific basis for ginseng as an adjunctive therapeutic drug or nutraceutical.

• Journal of Microbiology and Biotechnology
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• v.33 no.7
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• pp.864-874
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• 2023

Natural killer (NK) cell dysfunctions against hepatocellular carcinoma (HCC) in a hypoxic environment. Many solid tumors are present in a hypoxic condition, which changes the effector function of various immune cells. The transcription of hypoxic-inducible factors (HIFs) in cancer cells make it possible to adapt to their hypoxic environment and to escape the immune surveillance of NK cells. Recently, the correlation between the transcription of HIF-1α and pro-inflammatory cytokines has been reported. Interleukin (IL)-6 is higher in cancers with a highly invasive ability, and is closely related to the metastasis of cancers. This study showed that the expression of HIF-1α in HCC cells was associated with the presence of IL-6 in the environment of HCC-NK cells. Blocking of IL-6 by antibody in the HCC-NK interaction changed the production of several cytokines including TGF-β, IL-1, IL-18 and IL-21. Interestingly, in a co-culture of HIF-1α-expressed HCC cells and NK cells, blocking of IL-6 increased the production of IL-21 in their supernatants. In addition, the absence of IL-6 significantly enhanced the cytotoxic ability and the expression of the activating receptors (NKG2D, NKp44, and NKG2C) in NK cells to HIF-1α-expressed HCC cells. These effects might be made by the decreased expression of HIF-1α in HCC cells through the inhibited phosphorylation of STAT3. In conclusion, the absence of IL-6 in the interaction of HIF-1α-expressed HCC cells and NK cells could enhance the antitumor activity of NK cells to HCC cells.

• Food Science of Animal Resources
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• v.43 no.5
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• pp.859-876
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• 2023

It is known that animal-origin medicine could be one of effective treatment to remedy atopic dermatitis (AD) by controlling the cytokines. Cicadidae Periostracum (CP), the slough of Cryptotympana pustulata, has been frequently used for treating AD and skin affliction in traditional Korean Medicine. This study is aimed at investigating the ameliorating effects of CP on AD and its potential mechanism. The dinitrochlorobenzene sensitized mice were treated with CP for 2 weeks. The various biomarkers and the dermatitis scores presented that CP treatment can induce the visual and biological improvements of AD model. Pruritus, the most serious symptom of AD, which can cause repeated scratching behaviors and finally lead to lichenification, was reduced with CP treatment by regulating the inflammatory reactions. In addition, CP treatment diminished the number of mast cells that are known for causing inflammatory reactions. Moreover, it is proven that CP can decline secretion of interleukin-22, which means CP treatment has anti-inflammatory effects. CP treatment can correct the imbalance of helper T (Th)1 and Th2, downregulating thymic stromal lymphopoietin that leads to decrease of mRNA level of inflammatory cytokines. The crucial role of CP treatment is controlling of the Janus kinase 1/signal transducer and activator of transcription 3 pathway. In addition, CP treatment has the inhibitory effects on kallikrein related peptidase (KLK) 5 and KLK7. Taken together, CP treatment can ameliorate most symptoms and problems caused by AD disease, improving the AD patients' life quality.

• Journal of the Korea Convergence Society
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• v.13 no.1
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• pp.101-107
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• 2022

Tertomotide is a peptide vaccine developed for anti-cancer therapy. Since it has been found to ameliorate inflammatory symptoms in animal studies and clinical test, we investigated anti-inflammation activity of the tertomotide and the mechanism of action in monocyte in order to assess if tertomotide may serve as an anti-inflammatory agent by checking inflammatory cytokines and related signaling pathway following tertomotide treatment. We found that tertomotide reduced the level of pro-inflammatory cytokines such as TNF-α, IL-1β, IL-8 in LPS- or PMA-stimulated monocyte cell line and suppressed NF-κB signaling including the activation of ERK1/2 and P38 MAPK following TNF-α treatment. These results may correlate to the beneficial findings in animal studies, implicating that tertomotide may act as a potential anti-inflammatory agent. This study is an exemplary case for convergence that a computationally designed peptide for immunological purpose exerting unexpected biological activity may elicit novel anti-inflammatory drug.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.7 no.6
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• pp.75-83
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• 2004

Focusing on native plants that have high possibility of being introduced as rooftop material, this study was conducted to investigate extensive and easy-to-manage rooftop garden and to raise the utilization of native plants by verifying their growing response to artificial substrate soil depth and irrigation period. The study was conducted from March to September in 2002. Plants tested included Chrysanthemum zawadskii, Sedium middendorffianum, Thymus quinquecostatus, Allium senescens, and Dianthus superbus. Regarding soil depth, it was 5 cm and 10 cm. Irrigation period was non-irrigation, 1-week, 2-weeks, and 3- weeks, Its result is as follows; 1. In case of Sedum middendorffianum Maxim, mortality rate was 0% regardless of soil depth and irrigation period making it very suitable material for rooftop garden. 2. In case of Allium senescens L., mortality rate was 0% regardless of soil depth and irrigation period making it very suitable material for rooftop garden. Therefore, Provided that fertilizing is managed well, it is a plant that can be highly utilized.3. In case of Chrysanthemum zawadskii Herb. Subsp. (Nakai) Y. Lee Stat., the growth of top was lower in 10cm than in 5cm and it grew well in 10cm. When utilizing for rooftop garden, it would be desirable to keep minimum viable soil depth at over 10cm. If there is enough rainfall, soil and soil depth seem to have greater effect on growth than irrigation period does. 4. In case of Diauthus superbus L. var. longicalycinus (Maxim) Williams, rooting rate and growth were better in 10cm than in 5cm. Therefore, it is desirable to keep minimum soil depth at over 10cm. 5. In case of Thymus quinquecostatus Celak, the growth of top and flowering were better in 10cm than in 5cm. Therefore, it seems desirable to have minimum viable soil depth to be over 10cm. In conclusion, the most suitable species for rooftop garden are Sedium middendorffianum and Allium senescens in this experiment. However, Chrysanthemum zwadskii, Thymus quinquecostatus, and Dianthus chinensis also can be utilized greatly when irrigation is managed regularly in artificial mixed soil over 10cm.

• Development and Reproduction
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• v.12 no.1
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• pp.31-39
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• 2008

Neural tissue has limited intrinsic capacity of repair after injury, and the identification of alternate sources of neural stem cells has broad clinical potential. We isolated mesechymal-like stem cells from human adipose tissues (AT-MSCs), and studied on transdifferentiation-promoting conditions in neural cells. Dopaminergic and cholinergic neuron induction of AT-MSCs was also studied. Neural differentiation was induced by adding bFGF, EGF, dimethyl sulphoxide (DMSO) and butylated hydroxyanisole(BHA) in N2 Medium and N2 supplement. The immunoreactive cells for $\beta$-tubulin III, a neuron-specific marker, GFAP, an astrocyte marker, or Gal-C, an oligodendrocyte marker, were found. AT-MSCs treated with bFGF, SHH and FGF8 were differentiatied into dopaminergic neurons that were immunopositive for TH antibody. Differentiation of MSCs to cholinergic neurons was induced by combined treatment with basic fibroblast growth factor (bFGF), retinoic acid (RA) and sonic hedgehog (Shh). AT-MSCs treated with DMSO and BHA rapidly assumed the morphology of multipolar neurons. Both immunocytochemistry and RT-PCR analysis indicated that the expression of a number of neural markers including neuro D1, $\beta$-tubulin III, GFAP and nestinwas markedly elevated during this acute differentiation. While the stem cell markers such as SCF, C-kit, and Stat-3 were not expressed after preinduction medium culture, we confirmed the differentiation of dopaminergic and cholinergic neurons by TH/$\beta$-tubulin III or ChAT/ $\beta$-tubulin III positive cells. Conclusively, AT-MSCs can be differentiated into dopaminergic and cholinergic neuronsand these findings suggest that AT-MSCs are alternative cell source of treatment for neurodegenerative diseases.

• 한국방재학회:학술대회논문집
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• 2011.02a
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• pp.28-28
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• 2011

국내의 건축물에 관한 통계정보는 국토해양부에 의해서 공표된 통계연보로서 2002년부터 매년마다 제공되고 있다.(국토해양통계누리, https : //stat.mltm.go.kr/potal/stat/yearReport.do) 건축물의 통계연보는 과거부터 2002년까지 매 3년마다 공표된 것으로 알려지고 있다. 이 통계연보에는 건축물 현황과 건축허가착공 통계가 수록되어 있다. 건축물 현황에는 2009년 말을 기준으로 기존 건축물이 전체 6,618,131동으로서 용도별, 층수와 연면적별 그리고 소유구분별 등의 구분에 따라 건축물의 동수에 관한 통계자료가 포함돼 있다. 건축허가 및 착공통계에는 용도별과 구조재료별의 분류에 따라 건축물의 동수에 관한 자료가 신축, 증축 개축 이전 대수선, 용도변경 등으로 분류되어 수록돼 있다. 한편 국내에서 건축물에 대한 내진설계기준이 1988년에 제정됨에 따라 법제화되었다. 이때 내진설계의 의무 대상건축물은 6층 이상 또는 연면적 1만$m^2$이상인 건축물로 규정되었다. 그 이후 내진설계 대상 건축물이 1996년부터 아파트는 5층 이상으로, 판매시설은 연면적 5천$m^2$이상으로 확대되었고, 2000년부터 숙박시설 오피스텔 및 기숙사는 5층 이상으로 확대되었으며, 2005년부터 내진설계 의무화 대상이 3층 이상 또는 1천$m^2$이상으로 확대되었고, 2009년부터 3층 이상 건축물의 구조안전의 확인을 위한 세부절차를 규정하여 내진설계의 실효성을 확보하고 있다. 이와 같이 내진설계의 대상 건축물이 내진설계기준을 제정한 이후 현재까지 시대의 흐름에 따라 6층 이상에서 5층 이상으로 다시 3층 이상으로 계속 확대되어왔다. 이런 환경에서 현재 시점에서 사용 중인 기존 건축물 중에 내진설계가 적용되지 아니한 건축물은 1988년 3월 1일 이전에 건축허가 된 건축물과 그 이후에 건축허가 된 3층 내지 5층 이하인 저층 건축물의 두 가지로 구분할 수 있다. 이들의 내진설계가 미적용 된 건축물에 대해서는 원칙적으로 내진보강 대책 수립 및 추진이 필요한 실정이다. 국내에서는 현재 지진재해대책법에 따라 지진재해로부터 국민의 생명과 재산을 보호하기 위하여 기존 시설물에 대한 내진대책을 추진하는 정책이 시행되고 있다. 앞으로는 이 정책의 일환으로 기존 건축물의 내진성능 확보를 위한 내진보강 대책이 구체적으로 추진될 전망이다. 이와 같이 기존 건축물에 대한 내진보강 대책을 수립하는 데는 무엇보다 그 대상 건축물의 수와 구조형식에 관한 정보가 필요하다. 이는 내진보강의 방법과 소요비용이 건축물의 층수 및 구조형식별 동수에 따라 크게 달라지기 때문이다. 이런 관점에서 살펴볼 때 내진대책의 수립에 필수인 기존 저층 건축물의 층수 및 구조형식별 동수에 관한 통계자료를 현재 건축물의 현황통계에서 손쉽게 찾아 볼 수 있으면 좋겠는데 현실은 그렇지 못한 실정이다. 현재 건축물의 현황통계에는 저층 건축물에 해당하는 층수에 관한 구분이 연대에 따라 다르고 구체적인 층수를 구분하기 어렵게 불분명한 항목으로 구성된 것과 구조형식별 분류항목이 없는 형편이다. 반면에 건축허가 및 착공통계자료에는 구조재료별 건축물 동수와 연면적에 관한 자료가 수록되어있고, 건축물 층수에 따라 분류된 통계자료는 없다. 이 연구에서는, 기존 건축물에 대한 내진보강 대책을 수립하는데 필요한 저층 건축물의 층수 및 구조재료별 동수 등에 관한 구체적인 정보를 파악하기 위하여, 건축물 통계연보에 수록된 건축물 현황통계자료에서 불명확하거나 결여된 정보를 건축허가 및 착공 통계자료로부터 얻은 정보로 보완과 보충하여 제시하고 있다. 따라서 이 연구는 기존 건축물의 내진보강 대책 수립에 필요한 5층 이하의 저층 건축물에 관한 층수별 및 구조형식별 동수에 관한 연도별 통계자료를 추정하여 제안하는데 그 목적이 있다.

• Progress in Medical Physics
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• v.23 no.2
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• pp.81-90
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• 2012

The effect of setup uncertainties on CTV dose and the correlation between setup uncertainties and setup margin were evaluated by Monte Carlo based numerical simulation. Patient specific information of IMRT treatment plan for rectal cancer designed on the VARIAN Eclipse planning system was utilized for the Monte Carlo simulation program including the planned dose distribution and tumor volume information of a rectal cancer patient. The simulation program was developed for the purpose of the study on Linux environment using open source packages, GNU C++ and ROOT data analysis framework. All misalignments of patient setup were assumed to follow the central limit theorem. Thus systematic and random errors were generated according to the gaussian statistics with a given standard deviation as simulation input parameter. After the setup error simulations, the change of dose in CTV volume was analyzed with the simulation result. In order to verify the conventional margin recipe, the correlation between setup error and setup margin was compared with the margin formula developed on three dimensional conformal radiation therapy. The simulation was performed total 2,000 times for each simulation input of systematic and random errors independently. The size of standard deviation for generating patient setup errors was changed from 1 mm to 10 mm with 1 mm step. In case for the systematic error the minimum dose on CTV $D_{min}^{stat{\cdot}}$ was decreased from 100.4 to 72.50% and the mean dose $\bar{D}_{syst{\cdot}}$ was decreased from 100.45% to 97.88%. However the standard deviation of dose distribution in CTV volume was increased from 0.02% to 3.33%. The effect of random error gave the same result of a reduction of mean and minimum dose to CTV volume. It was found that the minimum dose on CTV volume $D_{min}^{rand{\cdot}}$ was reduced from 100.45% to 94.80% and the mean dose to CTV $\bar{D}_{rand{\cdot}}$ was decreased from 100.46% to 97.87%. Like systematic error, the standard deviation of CTV dose ${\Delta}D_{rand}$ was increased from 0.01% to 0.63%. After calculating a size of margin for each systematic and random error the "population ratio" was introduced and applied to verify margin recipe. It was found that the conventional margin formula satisfy margin object on IMRT treatment for rectal cancer. It is considered that the developed Monte-carlo based simulation program might be useful to study for patient setup error and dose coverage in CTV volume due to variations of margin size and setup error.