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http://dx.doi.org/10.6114/jkood.2022.35.2.001

Effects of Aster glehni Water Extracts on Skin Inflammation in HaCaT Keratinocytes  

Kim, Se-Jung (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Sangji University)
Lee, Kyou-Young (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Sangji University)
Kim, Geun-Lip (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Sangji University)
Hong, Chul-Hee (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Sangji University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.35, no.2, 2022 , pp. 1-12 More about this Journal
Abstract
Objectives : This study was conducted to verify the anti-inflammatory effects of Aster glehni water extracts in HaCaT keratinocytes. Methods : In this study, cell viability was confirmed by MTT assay. Production of TNF-α and IL-6 was determined by ELISA. mRNA expression of TARC and MDC were measusred by qRT-PCR. Also, expressions of p-JNK, JNK, p-ERK, ERK, p-p38, and p-38 were investigated by using western blot assay. Results : Aster glehni water extracts were not shown any significant cytotoxicity at 15.625-500㎍/㎖ in HaCaT keratinocytes. Aster glehni extracts inhibited the TNF-α and IL-6 production in HaCaT keratinocytes treated with TNF-α and IFN-γ. Also, expression of TARC, MDC, p-ERK, and p-STAT1 was decreased. Conclusions : These results suggest that Aster glehni water extracts have anti-inflammatory effects in HaCaT keratinocytes and can be applied to the development of anti-inflammatory treatment substances.
Keywords
Aster glehni; Anti inflammation; HaCaT keratinocytes; TNF-${\alpha}$; IL-6;
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Times Cited By KSCI : 3  (Citation Analysis)
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