• Asian-Australasian Journal of Animal Sciences
• /
• v.26 no.1
• /
• pp.30-35
• /
• 2013

The myostatin (MSTN) gene, as a negative regulator of skeletal muscle growth, has been proposed to be associated with production traits in farm animals. In the present study, a T/C variant at -125 bp (relative to ATG start codon) of 5'regulatory region of rabbit MSTN was identified by direct sequencing. Two hundred and twenty two rabbits, which were randomly sampled from 3 breeds (Ira rabbits, Champagne rabbits and Tianfu black rabbits), were genotyped by high-resolution melting (HRM). Comparing the genotyping results of 47 samples with direct sequencing, the HRM showed high sensitivity (0.96) and high specificity (0.98). In the three rabbit breeds, the allele C was the predominant allele. The polymorphic site showed high heterozygosity (He = 0.48) and high effective number of alleles (Ne = 1.91). The genetic diversity was reasonably informative (0.25

• Clinical and Experimental Pediatrics
• /
• v.46 no.10
• /
• pp.1024-1028
• /
• 2003

Influenza-associated encephalopathy is regarded as one of the major neurologic disease entities along with those of Reye syndrome, acute necrotizing encephalopathy, and myelitis which are known to be related to influenza virus, mostly type A. And it is being actively researched in Japan as it has caused a tremendous increase in the number of deaths from 1997 to 2002, but it has not been yet reported in the Korean pediatric medical community. It attacks those previously healthy children, who have not been vaccinated. Patients start with such symptoms as fever and common respiratory problems, but within 24 to 48 hours they suffer from seizures with acute mental deterioration, become worse, and suffer multiple organ failures including marked elevated transaminase levels as well as coagulopathy. It induces deaths in a couple of days after the symptoms appear or remains a serious neurologic sequelae. Confirmative diagnosis is used to demonstrate influenza viral infection. We report here a 37 month aged boy who was admitted to our hospital during the last influenza season under the diagnosis of influenza associated encephalopathy on the basis of serologic testing by hemagglutinin inhibition(HI). This is the first report confirmed by increased antibody titer of the influenza A virus in Korea.

• Korean Journal of Agricultural Science
• /
• v.48 no.3
• /
• pp.423-432
• /
• 2021

This experiment was conducted to investigate the effect of Cordyceps militaris with probiotics (CMP) supplementation on the growth performance, meat quality and storage characteristics, and cordycepin content in the meat. Sixty one-day-old broilers (Ross 308) were allotted to two treatment groups of 30 each. In addition, six broilers were randomly assigned to a cage in the two treatment groups. The two dietary treatments were as follows: Control (CON) and basal diet + 0.5% of CMP. Body weight and feed intake were measured on the 1st, 14th, and 28th days from the start of the experiment. On days 1 - 14, the supplementation of CMP improved (p < 0.05) the body weight gain (BWG) and feed conversion ratio (FCR). Additionally, the feed intake (FI) and FCR scores in the CMP groups improved (p < 0.01) compared to the CON during the entire period. For the meat quality characteristics, water holding capacity (WHC), cooking loss (CL), redness (a^*) in meat color value, and shearing force (SF) for the CMP group were improved (p < 0.01) compared to the CON group. For the meat storage characteristics, pH and thiobarbituric acid reactive substances (TBARS) were improved (p < 0.01) when the broilers were fed CMP compared to the CON group. Broilers fed CMP had a higher (p < 0.01) cordycepin content in the meat compared to the CON group. In conclusion, CMP improves the growth performance and meat quality of broilers.

• Journal of architectural history
• /
• v.21 no.6
• /
• pp.7-18
• /
• 2012

This study was aimed at analyzing the location and topology of the 'Gungmyo(宮廟)' and in Hanyang Doseong(漢陽都城) during 18th and 19th century. Based on the changes of royal processions(行幸) which had been done between the Gungmyo and the Palace, the Gungmyo can be a barometer of cognition where the city center was. Hanyang Doseong was the the capital of Joseon(朝鮮) which had established by king Taejo(太祖). The city had been organized with Gyeongbok-gung(景福宮) as the center. However, after the Imjin War(1592), Gyeongbok-gung was destroyed and urban space was reorganized with Changduk-gung(昌德宮) which reconstructed first. As most kings in late Joseon dynasty had mainly stayed in Changduk-gung, lots of Gungmyos where a memorial service for king's relatives had been held were established in and around the palace and the frequency of visiting the Gungmyo varied by political purpose. Therefore, the location of the Gungmyo and the rounte of parade were important to impress on the center of urban space. In 18th century, lots of King's procession had been done with Changduk-gung as departure point. The king used to start from Donwha-mun(敦化門), and the routes included main street around Changduk-gung. It shows that the urban center focused on the east of the city. On the other hand, when the king lived in Kyeonghee-gung(慶熙宮), a secondary palace in late Joseon dynasty, the parade started from Hungwha-mun(興化門) and the urban center was expended to the west. Since Gyeongbok-gung had reconstructed in 1865, recognition scheme of urban space had changed from Changduk-gung to Gyeongbok-gung as the center. When the Gungmyo relocated western side of Gyeongbok-gung, spatial proximity of the palace and relation with facilities around downtown fed into changing the route of king's parade.

• Korean Journal of Applied Biomechanics
• /
• v.27 no.2
• /
• pp.83-90
• /
• 2017

Objective: To investigate the effect of short-term vibration frequencies on muscle force generation capabilities. Method: Six healthy participants were recruited for this study and only their dominant leg was tested. The subjects were tested under five conditions of vibration frequencies with constant amplitude: 0 Hz (no vibration), 30 Hz, 60 Hz, and 90 Hz, and the vibration amplitude was 10 mm for all frequency conditions. The vibration was applied to the rectus femoris (RF). The subjects were then instructed to maintain a steady-state isometric knee joint torque (100 Nm) for the first 6 s. After the steady-state torque production, the subjects were required to produce isometric knee joint torque by leg extension as hard as possible with a start signal within the next 3 s. The vibration was applied for ~4 s starting from 1 s before initiation of the change in the steady-state knee joint torque. Results: The results showed that the maximum voluntary torque (MVT) of the knee joint increased with the vibration frequencies. On average, the MVTs were 756.47 Nm for 0 Hz (no vibration) and 809.61 Nm for 90 Hz. There was a significant positive correlation (r = 0.71) between the MVTs and integrated electromyograms (iEMGs). Further, the co-contraction indices (CCIs) were computed, which represent the ratio of the iEMGs of the antagonist muscle to the iEMGs of all involved muscles. There was a significant negative correlation (r = 0.62) between the CCIs and MVTs, which was accompanied by a significant positive correlation (r = 0.69) between the iEMGs of the vibrated muscle (RF). There was no significant correlation between the MVTs and iEMGs of the antagonist muscle. Conclusion: The results of this study suggest that the short-term vibration on the muscle increases the level of muscle activation possibly owing to the increased Ia afferent activities, which enhances the muscle force generation capability.

• Journal of Dental Rehabilitation and Applied Science
• /
• v.28 no.3
• /
• pp.319-326
• /
• 2012

For many years, ceramics have been used in fixed prosthodontics for achieving optimal esthetics. but, they have another use as well. Many studies today show ceramics can be used for biomaterials. In the beginning researchers made a start in the study of aluminium oxide and sapphire for biomaterial. The appearance of Zirconia began a new phase of research. Zirconia was introduced into implantology as an alternative to titanium, because of its white color, good mechanical properties and superior biocompatibility. But it is not easy to surface treatment in comparison with titanium. To overcome the limitation, interconnected porous bodies of zirconia were fabricated by sintering technique. And the technique of coating was developed. Therefore, some zirconia implants are currently available. It is thought that Research of biomaterials as a variety of puposes for the use of zirconia is looking very promising. The purpose of this paper reviews are to evaluation of zirconia as biomaterials.

• Parasites, Hosts and Diseases
• /
• v.54 no.6
• /
• pp.803-807
• /
• 2016

Toxascaris leonina is a common parasitic nematode of wild mammals and has significant impacts on the protection of rare wild animals. To analyze population genetic characteristics of T. leonina from South China tiger, its mitochondrial (mt) genome was sequenced. Its complete circular mt genome was 14,277 bp in length, including 12 proteincoding genes, 22 tRNA genes, 2 rRNA genes, and 2 non-coding regions. The nucleotide composition was biased toward A and T. The most common start codon and stop codon were TTG and TAG, and 4 genes ended with an incomplete stop codon. There were 13 intergenic regions ranging 1 to 10 bp in size. Phylogenetically, T. leonina from a South China tiger was close to canine T. leonina. This study reports for the first time a complete mt genome sequence of T. leonina from the South China tiger, and provides a scientific basis for studying the genetic diversity of nematodes between different hosts.

• The Journal of The Korea Institute of Intelligent Transport Systems
• /
• v.22 no.3
• /
• pp.51-61
• /
• 2023

Travel demand estimation of E-Scooter is the start point of solving the regional demand-supply imbalance problem and plays pivotal role in a linked transportation system such as Mobility-as-a-Service (a.k.a. MaaS). Most focuses on developing trip generation model of shared E-Scooter but it is no study on selection of an appropriate zone scale when it comes to estimating travel demand of E-Scooter. This paper aimed for selecting an optimal TAZ scale for developing trip distribution model for shared E-Scooter. The TAZ scale candidates were selected in 250m, 500m, 750m, 1,000m square grid. The shared E-Scooter usage historical data were utilized for calculating trip distance and time, and then applying to developing gravity model. Mean Squared Error (MSE) is applied for the verification step to select the best suitable gravity model by TAZ scale. As a result, 250m of TAZ scale is the best for describing practical trip distribution of shared E-Scooter among the candidates.

• Korean Journal of Agricultural Science
• /
• v.46 no.4
• /
• pp.885-895
• /
• 2019

Plant biotechnologists have long dreamed of technologies to manipulate genes in plants at will. This dream has come true partly through the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology, which now has been used to edit genes in several important crops. However, there are many restrictions in editing a gene precisely using the CRISPR/Cas9 technology because CRISPR/Cas9 may cause deletions or additions in some regions of the target gene. Several other technologies have been developed for gene targeting and precision editing. Among these, base editors might be the most practically and efficiently used compared to others. Base editors are tools which are able to cause a transition from cytosine into thymine, or from adenine into guanine very precisely on specific sequences. Cytosine base editors basically consist of nCas9, cytosine deaminase, and uracil DNA glycosylase inhibitor (UGI). Adenine base editors consist of nCas9 and adenine deaminase. These were first developed for human cells and have since also been applied successfully to crops. Base editors have been successfully applied for productivity improvement, fortification and herbicide resistance of crops. Thus, base editor technologies start to open a new era for precision gene editing or breeding in crops and might result in revolutionary changes in crop breeding and biotechnology.

• Journal of Korean Medical Ki-Gong Academy
• /
• v.11 no.1
• /
• pp.262-283
• /
• 2009

1. Anus is one of the nine holes, a soul store and on the lungs and the lungs, so the soulgate is said to meet. In addition, weight 450g, length 14.31inch, horizontal, and the 2.74inch, hit the road, the lungs and the reaction of the colon was found to represent the five viscera were dirty. 2. Spirit directly to the anus of the five viscera and the traffic outside and pass the information where I can get, and a close relation to the breathing, and each groups' je-hang-suk-sin-gong, je-hang-gong, anal breathing, jwa-sik training, jung-gi-dan-beop, martial arts method, Kegel exercises, breathing training, such as the anus is the name. 3.Anal way of breathing while breathing naturally slow down when you inhale air, tighten the anus and rectum to release when you breathe out, give way (or vice versa), the number of 5-20, he'll make you build up gradually. 4.Associated with respiratory training in Oriental medicine perspective, it is a Mac gi-gyeong eight vessel. In addition, out of gi-gyeong eight vessel in breathing training and more closely related to the Conception Vessel, Governor Vessel. 5.Governor Vessel, Conception Vessel and connect the energy up, down, left and right to communicate is the so-ju-cheon. To complete the Governor Vessel instantly without the energy leak from the Mir-yeo(尾閭) to reach the anus should be breathing. 6.Start the Conception Vessel Through fare Vessel hoeǔm, hoeǔm important acupuncture point can be activated by itself does not have the muscle to the hoeǔm around the anus energy and blood breathing helps circulation.