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MIRIS: Science Programs

  • Jeong, Woong-Seob;Matsumoto, Toshio;Seon, Kwangil;Pyo, Jeonghyun;Lee, Dae-Hee;Park, Youngsik;Ree, Chang Hee;Moon, Bongkon;Park, Sung-Joon;Nam, Uk-Won;Park, Jang-Hyun;Lee, Duk-Hang;Cha, Sang-Mok;Lee, Sungho;Yuk, In-Soo;Ahn, Kyungjin;Cho, Jungyeon;Lee, Hyung Mok;Han, Wonyong
    • The Bulletin of The Korean Astronomical Society
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    • v.37 no.2
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    • pp.97.2-97.2
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    • 2012
  • The main payload of Science and Technology Satellite 3 (STSAT-3), Multipurpose InfraRed Imaging System (MIRIS) is the first Korean infrared space mission to explore the near-infrared sky with a small astronomical instrument developed by KASI. The 8-cm passively cooled telescope with a wide field of view (3.67 deg. ${\times}$ 3.67 deg.) will be operated in the wavelength range from 0.9 to $2{\mu}m$. It will carry out wide-band imaging and the Paschen-${\alpha}$ emission line survey. After the calibration of MIRIS in our laboratory, MIRIS has been delivered to SaTReC and successfully assembled into the STSAT-3. The main purposes of MIRIS are to perform the observation of Cosmic Infrared Background (CIB) at two wide spectral bands (I and H band) and to survey the Galactic plane at $1.88{\mu}m$ wavelength, the Paschen-${\alpha}$ emission line. CIB observation enables us to reveal the nature of degree-scale CIB fluctuation detected by the IRTS (Infrared Telescope in Space) mission and to measure the absolute CIB level. The MIRIS will continuously monitor the seasonal variation of the zodiacal light towards the both north and south ecliptic poles for the purpose of calibration as well as the effective removal of zodiacal light. The Pashen-${\alpha}$ emission line survey of Galactic plane helps us to understand the origin of Warm Ionized Medium (WIM) and to find the physical properties of interstellar turbulence related to star formation. Here, we also discuss the observation plan with MIRIS.

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Comparative analysis of the transcriptomes and primary metabolite profiles of adventitious roots of five Panax ginseng cultivars

  • Lee, Yun Sun;Park, Hyun-Seung;Lee, Dong-Kyu;Jayakodi, Murukarthick;Kim, Nam-Hoon;Lee, Sang-Choon;Kundu, Atreyee;Lee, Dong-Yup;Kim, Young Chang;In, Jun Gyo;Kwon, Sung Won;Yang, Tae-Jin
    • Journal of Ginseng Research
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    • v.41 no.1
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    • pp.60-68
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    • 2017
  • Background: Various Panax ginseng cultivars exhibit a range of diversity for morphological and physiological traits. However, there are few studies on diversity of metabolic profiles and genetic background to understand the complex metabolic pathway in ginseng. Methods: To understand the complex metabolic pathway and related genes in ginseng, we tried to conduct integrated analysis of primary metabolite profiles and related gene expression using five ginseng cultivars showing different morphology. We investigated primary metabolite profiles via gas chromatography-mass spectrometry (GC-MS) and analyzed transcriptomes by Illumina sequencing using adventitious roots grown under the same conditions to elucidate the differences in metabolism underlying such genetic diversity. Results: GC-MS analysis revealed that primary metabolite profiling allowed us to classify the five cultivars into three independent groups and the grouping was also explained by eight major primary metabolites as biomarkers. We selected three cultivars (Chunpoong, Cheongsun, and Sunhyang) to represent each group and analyzed their transcriptomes. We inspected 100 unigenes involved in seven primary metabolite biosynthesis pathways and found that 21 unigenes encoding 15 enzymes were differentially expressed among the three cultivars. Integrated analysis of transcriptomes and metabolomes revealed that the ginseng cultivars differ in primary metabolites as well as in the putative genes involved in the complex process of primary metabolic pathways. Conclusion: Our data derived from this integrated analysis provide insights into the underlying complexity of genes and metabolites that co-regulate flux through these pathways in ginseng.

A Nonunique Composite Foreign Key-Based Approach to Fact Table Modeling and MDX Query Composing (비유일 외래키 조합 복합키 기반의 사실테이블 모델링과 MDX 쿼리문 작성법)

  • Yu, Han-Ju;Lee, Duck-Sung;Choi, In-Soo
    • Journal of the Korea Society of Computer and Information
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    • v.12 no.1 s.45
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    • pp.177-188
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    • 2007
  • A star schema consists of a central fact table, which is surrounded by one or more dimension tables. Each row in the fact table contains a multi-part primary key(or a composite foreign key) along with one or more columns containing various facts about the data stored in the row. Each of the composit foreign key components is related to a dimensional table. The combination of keys in the fact table creates a composite foreign key that is unique to the fact table record. The composite foreign key, however, is rarely unique to the fact table retold in real-world applications, particularly in financial applications. In order to make the composite foreign key be the determinant in real-world application, some precalculation might be performed in the SQL relational database, and cached in the OLAP database. However, there are many drawbacks to this approach. In some cases, this approach might give users the wrong results. In this paper, an approach to fact table modeling and related MDX query composing, which can be used in real-world applications without performing any precalculation and gives users the correct results, is proposed.

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A Multi-Epoch, Simultaneous Water and Methanol Maser Survey Toward Intermediate-Mass Young Stellar Objects

  • Bae, Jae-Han;Kim, Kee-Tae;Youn, So-Young;Kim, Won-Ju;Byun, Do-Young;Kang, Hyun-Woo;Oh, Chung-Sik
    • The Bulletin of The Korean Astronomical Society
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    • v.36 no.1
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    • pp.83.1-83.1
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    • 2011
  • We report multi-epoch, simultaneous 22 GHz water and 44 GHz Class I methanol maser line survey towards 180 intermediate-mass young stellar objects, including 14 Class 0, 19 Class I objects, and 147 Herbig Ae/Be stars. We detected $H_2O$ and $CH_3OH$ maser emission towards 16 (9%) and 10 (6%) sources with one new $H_2O$ and six new $CH_3OH$ maser sources. The detection rates of both masers rapidly decrease as the central (proto)stars evolve, which is contrary to the trends in high-mass star-forming regions. This suggests that the excitations of the two masers are closely related to the evolutionary stage of the central (proto)stars and the circumstellar environments. $H_2O$ maser velocities deviate on average 9 km s-1 from the ambient gas velocities whereas $CH_3OH$ maser velocities well match with. For both maser emissions, large velocity difference (${\mid}v_{H2O}-v_{sys}{\mid}\;>\; 10kms^{-1}\; and\;{\mid}v_{CH3OH}-v_{sys}{\mid}\;>\;1kms^{-1}$) is mostly confined to Class 0 objects. The formation and disappearance of $H_2O$ maser lines are frequent and the integrated intensities of them change up to two orders of magnitude. In contrast, $CH_3OH$ maser lines usually show no significant change in the intensity, shape, and velocity. This consistent with the previous suggestion that $H_2O$ maser emission originates from the base of an outflow while 44 GHz Class I $CH_3OH$ maser emission arises from the interaction region of the outflow with the ambient gas. The isotropic maser luminosities are well correlated with the bolometric luminosities of the central the objects. The fitted relations are $L_{H2O}=1.71{\ast}10^{-9}(L_{bol})^{0.97}$ and $L_{CH3OH}=1.71{\ast}10^{-10}(L_{bol})^{1.22}$.

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Theory and practice of the interlibrary loan service (상호대차(相互貸償) 제도의 이론(理論)과 응용)

  • Chun Myung Sook
    • Journal of the Korean Society for Library and Information Science
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    • v.13
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    • pp.39-71
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    • 1986
  • Interlibrary loan has been a very common library service in the developed countries but it is yet to be actualized formerly in Korea. As Korea is moving toward an information society where all the individual user's request in the library should be met, we need to understand, in detail, the theory and practice of interlibrary loan service. As various information technology has been applied to the libraries, the libraries became the open system. Thus, the libraries become more cooperative, form networks, and make interlibrary loan service available. This study is based on the following three hypothesis formulated from the systems theory. 1. If libraries cooperate each other and form some kind of networks by mail, computers or telecommunications, they become the open system. 2. If a library develops as the open system, interlibrary loan becomes available in the library. 3. If the interlibrary loan system works, the role of the librarians will be changed. The research was carried out by analyzing the various studies about interlibrary loan service and by examining the interlibrary loan systerns presently working in the U.S.. The conclusions are reached deductively from the data. Some of the terminology used are defined as follows: System: a network of interrelated procedures that are joined together to perform an activity or to accomplish a specific objective. It is, in effect, all the ingredient which make up the whole. Cooperation: Working together to benefit participant libraries. Network: a much more structured type of cooperation in which definite regions or areas are connected by electronic or other means to promote interlibrary loaning of materials, in-service traing & other sharing of resources. Interlibrary loan: the lending of books between libraries. The model of cooperation or networks was used to be (1) a star type, (2) a hieranchical type, (3) a distributed type or (4) the combination of aforementioned three. However, the development of the telecommunications and computers enables all kinds of libraires cooperate together. Interlibrary loan service starts with the needs of user's information. The information have to be logically accessed through bibliographies, computers, electronic mails and satellite communications. And the logical access requires the information technology and the libraries become cooperative. The physical access to the information follows next but only traditional method of mail and some commercial service are currently available for this purpose. Therefore, researches are needed to develop this physical access. If the libraries form networks, the microaspect of library changes accordingly as the macroaspect changes. If the libraries . cooperate to become one large world library, the librarians plan, organize, control library operations and report the results. And the librarians work inside and outside of the library to cooperate with other libraries. Only the cooperation of the libraries will enhance interlibrary loan and the Korean librarians have to be prepared to accept the new role of librarianship for the interlibrary loan service.

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Cloning, Expression, and Polymerization Assay of FtsZ Protein from Staphylococcus aureus (Staphylococcus aureus FtsZ의 클로닝, 발현 및 폴리머 형성 활성 분석)

  • Son, Sang Hyeon;Lee, Dong Yun;Kim, Ye Jun;Ko, Sooho;Cho, Seong Jun;Jung, Hyo Cheol;Lee, Hyung Ho
    • Microbiology and Biotechnology Letters
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    • v.40 no.3
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    • pp.274-277
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    • 2012
  • Cytokinesis is the final stage of cell division, dividing one mother cell into two daughter cells. For the cutting of a plasma membrane during bacterial cytokinesis, a tubulin homolog FtsZ protein is recruited from the cytoplasm to the division site. FtsZ protein polymerizes in a GTP-dependent manner and its N-terminal domain has a GTPase activity. In this study, we have begun to characterize FtsZ from Staphylococcus aureus (SA). Full-length SA FtsZ was cloned into pRSFDuet-1 vector and the clone was transformed into a BL21 (DE3) star cell. The recombinant SA FtsZ protein was purified using Ni-NTA affinity chromatography and dialysis. Using a spectrofluorometer, we showed that SA FtsZ undergoes a GTP-dependant polymerization in vitro. The polymer of the SA FtsZ protein disappeared after a few minutes, suggesting that the polymer is degraded as the GTP is consumed. This assay system may well be applied for inhibitor screening targeting S. aureus FtsZ.

Soluble Production of CMP-Neu5Ac Synthetase by Co-expression of Chaperone Proteins in Escherichia coli (샤페론 단백질 동시 발현기술을 이용한 수용성 CMP-Neu5Ac Synthetase 생산)

  • Choi, Hwa Young;Li, Ling;Cho, Seung Kee;Lee, Won-Heong;Seo, Jin-Ho;Han, Nam Soo
    • Microbiology and Biotechnology Letters
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    • v.42 no.2
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    • pp.190-193
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    • 2014
  • CMP-Neu5Ac synthetase is a key enzyme for the synthesis of CMP-Neu5Ac, which is an essential precursor of sialylated glycoconjugates. For the soluble expression of the CMP-Neu5Ac synthetase gene (neuA) from Escherichia coli K1, various heat shock proteins were co-expressed in E. coli BL21 (DE3) Star. In order to do this, a pG-KJE8 plasmid, encoding genes for GroEL-ES and DnaK-DnaJ-GrpE, was co-transformed with neuA and was expressed at $20^{\circ}C$ by the addition of 0.01 mM IPTG and 0.005 mg/ml L-arabinose. The co-expression of a variety of heat shock proteins resulted in the remarkably improved production of soluble CMP-Neu5Ac synthetase in E. coli.

MIRIS Science Missions

  • Jeong, Woong-Seob;Matsumoto, Toshio;Seon, Kwang-Il;Lee, Dae-Hee;Ree, Chang-Hee;Park, Young-Sik;Nam, Uk-Won;Pyo, Jeong-Hyun;Moon, Bong-Kon;Park, Sung-Joon;Cha, Sang-Mok;Park, Jang-Hyun;Lee, Duk-Hang;Lee, Sung-Ho;Yuk, In-Soo;Ahn, Kyung-Jin;Cho, Jung-Yeon;Lee, Hyung-Mok;Han, Won-Yong
    • Bulletin of the Korean Space Science Society
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    • 2010.04a
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    • pp.26.4-27
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    • 2010
  • The main payload of STSAT-3 (Science and Technology Satellite 3), MIRIS (Multipurpose InfraRed Imaging System) is the first Korean infrared space mission to explore the near-infrared sky with a small astronomical instrument, which is being developed by KASI. The 8-cm passively cooled telescope with a wide field of view (3.67 deg. $\times$ 3.67 deg.) will be operated in the wavelength range from 0.9 to $2{\mu}m$. It will carry out wide field imaging and the emission line survey. The main purposes of MIRIS are to perform the Cosmic Infrared Background (CIB) observation at two wide spectral bands (I and H band) and to survey the Galactic plane at $1.88{\mu}m$ wavelength, the Paschen-$\alpha$ emission line. CIB observation enables us to reveal the nature of degreescale CIB fluctuation detected by the IRTS (Infrared Telescope in Space) mission and to measure the absolute CIB level. The Pashen-$\alpha$ emission line survey of Galactic plane helps us to understand the origin of Warm Ionized Medium (WIM) and to find the physical properties of interstellar turbulence related to star formation. Here, we also discuss the observation plan with MIRIS.

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A Wireless Sensor Network Systems to Identify User and Detect Location Transition for Smart Home (지능형 주택을 위한 구성원 식별 및 위치 이동 감지 센서 네트워크 시스템)

  • Lee, Seon-Woo;Yang, Seung-Yong
    • Journal of KIISE:Information Networking
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    • v.37 no.5
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    • pp.396-402
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    • 2010
  • The tracking of current location of residents is an essential requirement for context-aware service of smart houses. This paper presents a wireless sensor network system which could detect location transition such as entrance and exit to a room and also identify the user who passed the room, without duty of wearing any sort of tag. We designed new sensor node to solve the problem of short operation lifetime of previous work[1] which has two pyroelectric infrared (PIR) sensors and an ultrasonic sensor, as well as a 2.4 GHz radio frequency wireless transceiver. The proposed user identification method is to discriminate a person based on his/her height by using an ultrasonic sensor. The detection idea of entering/exiting behavior is based on order of triggering of two PIR sensors. The topology of the developed wireless sensor network system is simple star structure in which each sensor node is connected to one sink node directly. We evaluated the proposed sensing system with a set of experiments for three subjects in a model house. The experimental result shows that the averaged recognition rate of user identification is 81.3% for three persons. and perfect entering/exiting behavior detection performance.

Characteristics of Cucumber mosaic virus-GTN and Resistance Evaluation of Chilli Pepper Cultivars to Two Cucumber mosaic virus Isolates (고추에서 분리한 오이모자이크바이러스(CMV-GTN)의 특성과 고추 품종의 저항성 평가)

  • Choi, Gug-Seoun;Kwon, Sun-Jung;Choi, Seung-Kook;Cho, In-Sook;Yoon, Ju-Yeon
    • Research in Plant Disease
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    • v.21 no.2
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    • pp.99-102
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    • 2015
  • Cucumber mosaic virus (CMV) is one of the most destructive viruses in chilli pepper. An isolate of CMV was obtained from the chilli pepper cv. Chungyang showing top necrosis symptom in 2013 and designated as CMV-GTN. CMV-GTN was compared with the well-characterized isolate, CMV-Ca-P1, by investigating their amino acid sequences of the coat protein (CP) and biological reactions in several host plants. The CP of CMV-Ca-P1 composed of 217 amino acids but that of CMV-GTN composed of 218 amino acids by including additional valine in the $57^{th}$ amino acid position. Amino acid sequence similarity of the CP gene among CMV-GTN and other CMV isolates recorded in the GeneBank database ranged from 96% to 99%. CMV-GTN was selected as a representative isolate to screen the resistance pepper cultivars to CMV because it was highly pathogenic to tomatoes and peppers upon biological assays. The virulence of CMV-GTN was tested on 135 pepper cultivars which has been bred in Korea and compared with that of CMV-Ca-P1. Only the cv. Premium was resistant and three cvs. Hot star, Kaiser, and Good choice were moderately resistant to CMV-GTN, whereas two cvs. Baerotta and Kaiser were resistant to CMV-Ca-P1.