• Journal of Dairy Science and Biotechnology
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• v.17 no.1
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• pp.11-15
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• 1999

The objective of this study was to extract and purity the antibacterial agent from the fermented milk with Lactobacillus helveticus CH-1. The extraction and purification of antibacterial agent from the Lb. helveticus fermented milk were carried out by methanol extraction, acetone extraction, Sephadex G-200 gel filteration and thin layer chromatography and the results were as followings. The antibacterial activity of methanol-acetone extraction showed antibacterial activity against test organisms, B. subtilis, E. coli, Pseu. fluorescens, Sal. typhimurium, Shi. flexneri, and Sta. aureus. Sephadex G-200 gel chromatography showed only antibacterial activity from 33 to 37th fractions of 60 fractions. The agent purified from TLC plate confirmed the antibacterial activity by the means of bioautography.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.6
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• pp.392-399
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• 2000

This study was carried out to investigate the physicochemical characteristics of corn silks. Pollination of corn silks was finished within three days after silking, and elongation of open pollinated corn silks was stopped, but unpollinated silks elongated until eight days after silking. Moisture contents of corn silks were about 92-94% at silking stage, but continuously decreased. these were about 70-75% at 30 days after silking. Chlorophyll b was higher than chlorophyll a in corn silks, and chlorophyll a/b ratios of four hybrids were high in this order : silage ＞ sweet ＞ super sweet ＞ waxy corn. Free sugars in corn silks were mainly fructose, glucose and maltose. Their composition rate was 55% of glucose, 42% of fructose and 4% of maltose. Water soluble solid (Brix %) contents of unpollinated corn silks were ranged from 13.7 to 16.8 Brix % and pollinated corn silks were from 12.6 to 13.7 Brix %. Phytic, oxalic, malic. shikimic, glutaric and acetic acid were detected on corn silks. Phytic, oxalic and glutaric acid were considered as a major organic acids in corn silks. Amino acids in corn silks were high in this order : waxy ＞ silage ＞ sweet ＞sweet corn. Serine, glycine and thereoine were contained more than 10%, and five amino acids such as aspartic, glutaric, arginine, alanine and proline were ranged about 5 to 8%. Methionine and cystine, amino acids containing sulfur were contained only small quantity as about 2.1% and 1.3%, respectively. Acetaldehyde, ethanol, acetone, DMS, isobutylaldehyde, cis-3-hexanol, 3-hexe-1-ol, acetate, trans-2-hexanol and pentanol were detected as the volatile components in corn silks, but acetaldehyde and DMS were major volitiles in silage corn silks, and acetaldehyde, ethanol and DMS were major volitiles in waxy corn silks. The length of corn silks was a positively correlated with organic acids (r=${0.556}^*$), and a negatively correlated with amino acids (r=${-0.514}^*$), respectively. Free sugars were positively correlated with all characteristics tested and significantly correlated with organic acid (r=TEX>${0.703}^{**}$), and flavonoids (r=TEX>${0.544}^*$). Chlorophyll was significantly correlated with flavonoid contents (r=TEX>${0.523}^*$). For this reason chlorophyll was evaluated as an indirect selection character for the high flavonoid containing varieties.

• Korean Journal of Agricultural Science
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• v.23 no.1
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• pp.80-89
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• 1996

The expression and secretion of human lactoferrin (hLf) in Sacclnromyces diastaticus were performed. 1. For the secretion of hLf in yeast, recombinant plasmid pYEGLf was constructed using promoter, secretion signal sequence of glucoamylase I gene (STA1) and transcriptional terminator of GAL7 gene. 2. Each correct recombinant plasmid was selected by mini-preparation of plasmid DNA from E coli transformant and restriction enzyme digestion analysis. The selected plasmids, pYEGLf, were transformed into S. diastaticus YIY345 as a expression host, respectively. 3. Western blot analysis using rabbit anti-hLf was carried out to identify expressed hLf. Positive signals were shown in culture supernatant of pYEGLf transformant. 4. About $100{\mu}g-1mg$ of concentrated culture supernatant of positive clone were loaded on paper disc and tested for the antimicrobial activity against E coli. However, no activity was observed. We concluded that this fact results from low concentration of hLf secreted from yeast, compared with the fact that MIC of hLf is as high as $3mg/m{\ell}$. Therefore, the purification of secreted hLf may be require to investigate the antimicrobial activity. From this study, the feasibility of low-cost production of sufficient quantities of human lactofferin for nutritional and therapeutical applications were suggested.

• Korean Journal of Soil Science and Fertilizer
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• v.24 no.3
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• pp.219-224
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• 1991

An acid tolerant R. meliloti "YA03" which was characterized through both acidified tube and pot soil experiment, was examined on its inoculation effect to alfalfa[Medicago sativa (L.)]cv. Vernal on hilly acid soil. It was conducted for 2 years to evaluate inoculation residual effect and there were two-fertilizer factors of nitrogen(0.8kg/10a) and lime(0,162kg/10a)as urea and $Ca(OH)_2$, respectively. The results obtained were summarized as follows : 1. In the 1st-year experiment, YA03 inoculated without nitrogen and lime application showed higher symbiotic effectiveness than check strains, YA026 and Rm2011 in nodule mass, $N_2$-fixing activity, and dry matter yield, but with N 8kg/10a application lower effectiveness than Rm2011. 2. In the 2nd-year experiment, Alfalfa dry matter yield by YA03 inoculation with N application(without lime) was 74% higher than that(276 kg/10a) in the 1st year, and the value showed 166%, 20%, and 21% increments as compared with noninoculated control(180kg/10a). YA026(399kg/10a), and Rm2011(397kg/10a), respectively. 3. In the 2nd-year experiment of N applicated plote(without lime), especially, YA03 showed higher shoot nitrogen(7.7kg N/10a/Yr.) fixed and soil rhizobial population($1{\times}10^4\;cells/g.\;soil$) than check strains. 4. On the basis of lime application, application effect of three major fertilizers with YA03 inoculation on alfalfa yield and soil rhizobial population was in the order of $N+P_2O_5+K_2O>P_2O_5+K_2O>P_2O_5+K_2O$. 5. On the whole, alfalfa yield by acid tolerant R. meliloti YA03 inoculation on hilly acid soil was enhanced with nitrogen application, and besides it appeared 44% higher with lime than without lime.

• Korean Journal of Microbiology
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• v.31 no.3
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• pp.203-207
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• 1993

Five different secretion vectors were constructed by varying the signal sequences and .alpha.-antitrypsin (.alpha.1-AT) a numan secretory protein, was produced from yeast cells. The signal sequences used are those of acid phosphatase (PH05) and .alpha.-factor (M f.alphal1) of Saccharomyces cerevisiae, glucoamylase (STA1) of Saccharomyces diastaticus, and human .alpha.1-AT. Four vectors directed the efficient secretion of .alpha.1-AT ito the culture media. The secretion vector carrying the glucoamylase signal sequence (pGAT11) showed the highest efficiency of secretion. About 70% of .alpha.1-AT produce dwere secreted into the media. The endo H treatment of partially purified .alpha.1-AT indicates that the secreted .alpha.1-AT appeared to be glycosylated. This glycosylation pattern was altered when amino acid substitution mutations were introduced at the three glycosylation sites of .alpha.-AT.

• Computers and Concrete
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• v.14 no.4
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• pp.405-417
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• 2014

The poor seismic performance of reinforced concrete buildings during the latest earthquakes has become a serious issue in the building industry in Turkey. This case, designing new buildings without structural irregularities against earthquake loads reveals to be quite significant. This study mainly is focused on the effects of different torsional irregularities on construction costs and earthquakes performance of reinforced concrete buildings. In that respect, structural torsional irregularities are investigated based on the Turkish Earthquake Code. The study consists of major eight main parametric models. In this models consist of totally 49 models together with the variations in the number of storey. With this purpose, the earthquake performances and construction costs (especially steel quantities) of reinforced concrete buildings which having different structural torsional irregularities were obtained with the help of Sta4-CAD program. Each model has been analyzed by both the methods of equivalent earthquake loading and dynamic analysis. The obtained results reveal that the model-1 which has lower torsional irregularity coefficient shows the best earthquake performance owing to its regular plan geometry. Also, economical comparisons on costs of the torsional irregularity are performed, and results-recommendations are given.

• Proceedings of the Korean Information Science Society Conference
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• 2002.04a
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• pp.301-303
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• 2002

요즘 사무 빌딩이나 공항에서의 ISM(Industrial Scientific and Medical) 밴드 사용이 증가함에 따라 동일 주파수 대역을 사용하는 BT(Bluetooth)와 무선 랜은 상호 충돌 발생이 일어난다. 이러만 문제점이 최근 연구과 제로 등장하고 있다. 본 논문은 채널 간섭의 모델에 의거하는 UL(unlicensed) ISM 주파수 대역에서 BT와 802.11에 있어서 충돌 평가 모델에 의한 간섭을 분석 한다. 전파 평가 모델 방법론에 의한 AP와 STA간의 범위에 따른 BT 간섭 의 수와 허용할 수 있는 최대 BT 신호를 알아본다. 그리고, BT를 바탕으로 한 액세스 네트워의 실행에서 802.l1b 시스템에 관한 BT의 기본 간섭 메커리즘 및 간섭이 어느 정도 미치는지에 관한 예비 결과를 나타낸다.

• Journal of Korean Institute of Industrial Engineers
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• v.24 no.2
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• pp.175-183
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• 1998

When a product is designed, tolerances must be given to the product so that required functions are guaranteed and production costs are minimized. In this research, a model is suggested which allocates tolerances to components optimally according to the STA(Statistical Tolerance Allocation) method. Taking into account the concept that dimensional errors have characteristics of statistical distributions, this model presents the discrete pseudo-boolean approach for the tolerance optimization by minimizing the tolerance cost and the quality loss cost. In this approach, two methods are proposed for the reduction of the problem scale; 1) a method for converting the minimization model for casts into the maximization model for cost savings, and 2) procedures to reduce the number of constraints and variables.

• Advances in concrete construction
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• v.10 no.4
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• pp.333-343
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• 2020

In the architectural design process, the selection and configuration of the structural system significantly affect the earthquake behaviours of the reinforced concrete buildings. The main purpose of this study, the effects on the earthquake performances and the rough construction cost of the buildings of the slab type in reinforced concrete buildings are to examine comparatively for different local soil classes. The results obtained from this study have been determined that the building model having slabs with beams is safer compared to other types of slabs, especially when considering the vertical bearing structural elements (columns). It also shows that other types of slab, except for slab with beams, reduce the earthquake performances of reinforced concrete buildings, increase the displacement values, 1st natural vibration period values and the cost of rough construction. This matter reveals that slab type is quite important and the preference of beamed slabs in reinforced concrete buildings to be constructed in earthquake zones would be more appropriate in terms of safety and cost.

• Proceedings of the Korean Vacuum Society Conference
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• 2010.08a
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• pp.200-200
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• 2010

이 논문에서는 field-effect mobility를 향상시키기 위해 triple-layer (SiNx/SiO2/SiOxNy stack 구조)를 gate dielectric material 로 LTPS TFTs에 적용하였다. 이는 플라즈마 처리 기법과 적층구조의 효과적인 in-situ 공정을 이용하여 interface trap과 mobile charge를 낮추어 높은 이동도의 결과를 생각하고 실험하였다. 실험은 SiO2 gatedielectric과 triple-gate dielectric의 C-V curve를 1 MHz의 주파수에서 측정하였다. 또한 Transfer characteristics를 single SiO2 gatedielectric과 triple-gate dielectric of SiNx/SiO2/SiOxNy를 STA 장비를 이용해 측정하였다. 위의 측정을 통해 threshold voltage, mobility, subtheshold swing, driving current, ON/OFF current ratio를 비교 분석하였다.