• Korean Journal of Microbiology
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• v.31 no.1
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• pp.48-53
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• 1993

For the purpose to improve the glucoamylase productivity of Saccharomyces diastaticus, we integrated STA 1 gene into chromosomal DNA of S. diastaticus using YIp vector. After construction of Ylp-STA by the subcloning of STAI (5.3 kb) into YIp5 vector, S. diastaticus GMT-II(a. ura3. STAJ) was transformed by Ylp-STA through homologous recombination at the chromosomal STAJ gene. So we obtained the tram formants that glucoamylase productivity was increased maximum six fold. These strains transformed by the multi-copy integration of Ylp-STA in chromosomal DNA were confirmed by Southern hybridization. And the integrated Ylp-STA was maintained stably during 30 mitotic divisions.

• Proceedings of the Korean Nutrition Society Conference
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• 2003.05a
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• pp.125.1-125.1
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• 2003

• Journal of The Korean Society of Agricultural Engineers
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• v.49 no.5
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• pp.11-20
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• 2007

In this study, we reviewed the optimal space for the fishway and fish storage establishment in the section between the downstream of the Hwacheon dam and the confluence of the Pungsan river. The hydraulic stability of these facilities was also examined. In the section between the downstream of Hwacheon dam and the confluence of Pungsan river, the release flow of Hwacheon dam was set at $5,495m^3/s,\;6,110m^3/s$ and $7,537m^3/s$, and numerical simulations were executed for each instance using the HEC-RAS model and RMA-2. In the analysis of the flow velocity distributions in the subject section by release flows of Hwacheon dam, it was shown that there was over 5.0m/s of high flow velocity at Sta. $#1{\sim}Sta.$ #3, Sta. $#4{\sim}Sta.$ #6, and Sta. $#12{\sim}Sta.$ #13 of the sections analyzed in this study. The Sta. $#0{\sim}Sta.$ #1 was considered appropriate for the fishway and fish storage establishment. As seen in the results of the numerical stability review by release flow conditions of Hwacheon dam subject to the selected available areas fur fishway and fish storage, the topographically available section for the establishment of fishway and fish storage exists at Sta. $#1{\sim}Sta.$ #3 when the release flow of Hwacheon dam is $5,495m^3/s$, whereas Sta. $#0{\sim}Sta.$ #1 should be reviewed for the subject section for the establishment of fishway at release flows of $6,110m^3/s$ and $7,537m^3/s$, in addition to the figures gathered at the $5,495m^3/s$ release flow. Finally, the available section for the establishment of fishway and fish storage was determined to be Sta. $#0{\sim}Sta.$ #3. Further, if fishway and fish storage are established in the outer bank within this section, the high flow velocity section should be avoided. An alternative would be to establish for the fishway in the inner band on the section of Sta. #1 or Sta. #3.

• Korean Journal of Microbiology
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• v.30 no.5
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• pp.403-409
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• 1992

For the development of a glucanolytic yeast strain. the seceretion of endo-1.4-$\beta$-D-glucanase (CMCase) of Bacillus subtilis was performed in yeast using glucoamylase gene (STA1) of Saccharomyces diastaticus. A 1.7 kb-DNA fragment of STA1 gene containing authentic promoter, signal sequence, threonine serine-rich (TS) region and N-terminal region (98 amino acids) of mature glucoamylase was ligated to YEp 24. E. coli-yeast shuttle vector. And then. CMCase structural gene of B. subtilis was fused in frame with the 1.7 kb-DNA fragment of STA1 gene, resulting in recombinant plasmid pYES('24. Yeast transformant harboring pYESC24 had no CMCase activity. So. we deleted TS region and N-terminal region of mature glucoamylase existing between signal sequence and CMCase structural gene in pYESC24. consequently constructed recombinant plasmid pYESC11. The yeast transformed with the newly constructed recombinant plasmid pYESC11 efficiently secreted CMCase to extracellular medium. After 4 days culture. total CMCase activity of this transformant was 44.7 units/ml and over 93% of total CMCase activity was detected in culture supernatant.

• Proceedings of the Korean Society of Crop Science Conference
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• 2000.04a
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• pp.416-417
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• 2000

• Journal of Environmental Science International
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• v.4 no.5
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• pp.103-103
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• 1995

In the Chinhae Bay, Korea, sedimentation rates and sedimentary record of anthropogenic metal loads were determined by $^{210}Pb$ dating and heavy metal analysis of four sediment cores . The sedimentation rates varied from 0.16g/$cm^2$/yr(3.1mm/yr) at Sta. Ct, located within narrow waterway to 0.24g/$cm^2$/yr(4.8mm/yr) at Sta. Cl, located in Haengam Bay. Maximum contents of Mn, Zn, Cu and Cr were observed at Sta. C2 located near the mouth of Masan Bay, while minimum contents were observed at Sta. CB. Mn/Fe ratios at Sta. C2 and Sta. C4 showed gradually increasing and decreasing downward, respectively, in the upper layer of sediment cores. This suggests that Mn may be diagenetically redistributed in highly reduced environment. At Sta. C2, the concentrations of Zn and Cu began to increase from 1920s by anthropogenic input and have been remarkablely increasing since mid 1960s. At Sta. C3, located near Sungpo, anthropogenic input of these two elements has also slightly increased after 1970s. However, pollution of these two elements was not significant in Haengam Bay(Sta. Cl) and Chiljun watenway(Sta. C4). The pollution of Co, Ni and Cr was not remarkable in all core samples except surface sediment of Sta. C2. The total input of anthropogenic Zn and Cu since 1920s was estimated to be 28∼792 ㎍/cm2 and 0∼168㎍/cm2, respectively. Sta. C2 showed remarkablely higher values relative to other stations: anthropogenic loads of Zn and Cu constituted 27% and 29% of the total sedimentary inventories at the present day, respectively. Fe, Ni, Cr and Co contents showed good correlation(r>0.8) with each other. Anthropogenic Zn and Cu also showed a very good positive correlation(>0.9). However, correlation between these two group of element was quite scattered, indicating different sources and geochemical behaviors.

• Journal of Environmental Science International
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• v.4 no.5
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• pp.489-500
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• 1995

In the Chinhae Bay, Korea, sedimentation rates and sedimentary record of anthropogenic metal loads were determined by $^{210}Pb$ dating and heavy metal analysis of four sediment cores . The sedimentation rates varied from 0.16g/$cm^2$/yr(3.1mm/yr) at Sta. Ct, located within narrow waterway to 0.24g/$cm^2$/yr(4.8mm/yr) at Sta. Cl, located in Haengam Bay. Maximum contents of Mn, Zn, Cu and Cr were observed at Sta. C2 located near the mouth of Masan Bay, while minimum contents were observed at Sta. CB. Mn/Fe ratios at Sta. C2 and Sta. C4 showed gradually increasing and decreasing downward, respectively, in the upper layer of sediment cores. This suggests that Mn may be diagenetically redistributed in highly reduced environment. At Sta. C2, the concentrations of Zn and Cu began to increase from 1920s by anthropogenic input and have been remarkablely increasing since mid 1960s. At Sta. C3, located near Sungpo, anthropogenic input of these two elements has also slightly increased after 1970s. However, pollution of these two elements was not significant in Haengam Bay(Sta. Cl) and Chiljun watenway(Sta. C4). The pollution of Co, Ni and Cr was not remarkable in all core samples except surface sediment of Sta. C2. The total input of anthropogenic Zn and Cu since 1920s was estimated to be 28~792 $\mu\textrm{g}$/cm2 and 0~168$\mu\textrm{g}$/cm2, respectively. Sta. C2 showed remarkablely higher values relative to other stations: anthropogenic loads of Zn and Cu constituted 27% and 29% of the total sedimentary inventories at the present day, respectively. Fe, Ni, Cr and Co contents showed good correlation(r>0.8) with each other. Anthropogenic Zn and Cu also showed a very good positive correlation(>0.9). However, correlation between these two group of element was quite scattered, indicating different sources and geochemical behaviors.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1993.10a
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• pp.7-8
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• 1993

1970년대의 오일쇼크는 시설재배 경영자로 하여금 성에너지를 효율적으로 이용케하는 연구에 박차를 가했다. 따라서 1970년대 후반에 남부지방에서는 지하수열을 이용하는 방법으로서 소위 Water curtain의 방법을 이용한 시설의 난방 방법이 넓게 확산되어갔다. Water curtain의 난방 방법은 통상 15$^{\circ}C$ 전후 수원의 지하수를 이용해 curtain 위에 살수함으로써 동기에 1$0^{\circ}C$ 정도의 온실의 유지가 가능하여 비교적 저염성 채소(토마토, 딸기 등)의 재배에 이용되고 있다. (중략)

• Journal of the Korean Society of Systems Engineering
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• v.19 no.1
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• pp.29-38
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• 2023

Independent Safety Assessment (ISA) is a third-party assessment that is to confirm that the system satisfies the safety requirements in the defined operational context. The process of this assessment often brings about many complex arguments that should be supported by evidence and justification. The communication between arguments and evidence is of the most importance in the context of safety case. This study illustrates how NOR-STA can be used for ISA process, showing the effective structure of safety compliance. The study outlines the steps to breaks down the top goal into many elements such as arguments, sub-goals, justification, context and assumptions. It concludes that the evidence-based safety conformance process utilizing NOR-STA provides a more effective and systematic representation of the independent safety assessment process in conformance cases.

• Convergence Security Journal
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• v.11 no.1
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• pp.33-38
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• 2011

Current IEEE 802.11 standard is very vulnerable that between the AP and STA authentication and security mechanisms is widely known. Therefore, IEEE has proposed security architecture RSN (Robust Security Network) for 802.11. RSN is used the access control, authentication, and key management based on the IEEE 802.1X standard. In this paper, IEEE 802.1X or 802.11 a combination of several models proposed for the vulnerability, and session hijacking or MiM (Man-in the-Middle) attacks to respond, the authentication mechanism Was designed to the access control between the STA and the AP.