Purpose In a cyclosporine experiment using a robotic liquid handing system has found a deviation of its standard curve and low reproducibility of patients's results. The difference of the test is that methanol is mixed with samples and the extractions are used for the test. Therefore, we assumed that the abnormal test results came from using methanol and conducted this test. In a manual of a robotic liquid handling system mentions that we can choose several setting parameters depending on the viscosity of the liquids being used, the size of the sampling tips and the motor speeds that you elect to use but there's no exact order. This study was undertaken to confirm pipetting ability depending on types of liquids and investigate proper setting parameters for the optimum dispensing ability. Materials and Methods 4types of liquids(water, serum, methanol, PEG 6000(25%)) and $TSH^{125}I$ tracer(515 kBq) are used to confirm pipetting ability. 29 specimens for Cyclosporine test are used to compare results. Prepare 8 plastic tubes for each of the liquids and with multi pipette $400{\mu}l$ of each liquid is dispensed to 8 tubes and $100{\mu}l$ of $TSH^{125}I$ tracer are dispensed to all of the tubes. From the prepared samples, $100{\mu}l$ of liquids are dispensed using a robotic liquid handing system, counted and calculated its CV(%) depending on types of liquids. And then by adjusting several setting parameters(air gap, dispense time, delay time) the change of the CV(%)are calcutated and finds optimum setting parameters. 29 specimens are tested with 3 methods. The first(A) is manual method and the second(B) is used robotic liquid handling system with existing parameters. The third(C) is used robotic liquid handling system with adjusted parameters. Pipetting ability depending on types of liquids is assessed with CV(%). On the basis of (A), patients's test results are compared (A)and(B), (A)and(C) and they are assessed with %RE(%Relative error) and %Diff(%Difference). Results The CV(%) of the CPM depending on liquid types were water 0.88, serum 0.95, methanol 10.22 and PEG 0.68. As expected dispensing of methanol using a liquid handling system was the problem and others were good. The methanol's dispensing were conducted by adjusting several setting parameters. When transport air gap 0 was adjusted to 2 and 5, CV(%) were 20.16, 12.54 and when system air gap 0 was adjusted to 2 and 5, CV(%) were 8.94, 1.36. When adjusted to system air gap 2, transport air gap 2 was 12.96 and adjusted to system air gap 5, Transport air gap 5 was 1.33. When dispense speed was adjusted 300 to 100, CV(%) was 13.32 and when dispense delay was adjusted 200 to 100 was 13.55. When compared (B) to (A), the result increased 99.44% and %RE was 93.59%. When compared (C-system air gap was adjusted 0 to 5) to (A), the result increased 6.75% and %RE was 5.10%. Conclusion Adjusting speed and delay time of aspiration and dispense was meaningless but changing system air gap was effective. By adjusting several parameters proper value was found and it affected the practical result of the experiment. To optimize the system active efforts are needed through the test and in case of dispensing new types of liquids proper test is required to check the liquid is suitable for using the equipment.
Journal of Korean Society of Environmental Engineers
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v.36
no.10
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pp.685-690
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2014
The treatment of combined sewer overflow (CSO) is one of potential concerns in domestic wastewater treatment in Korea due to the pre-announce of CSO regulations. This work investigated the effects of disinfectant (NaOCl) concentration (0.11 to 4.0 mg $Cl_2/L$), pH (6.5 to 8.0), temperature (15 to $25^{\circ}C$), ammonia (10 to 41 mg N/L), and suspended solids (91 to 271 mg SS/L) on the chlorine disinfection of CSO. The effect of NaOCl concentration on the pseudo-$1^{st}$ order reaction rate for total coliform inactivation was described well with a saturation-type model with the half-velocity constant of 1.212 mg/L. The total coliform inactivation reaction rate decreased with SS and pH, and increased with temperature. Ammonia in the examined range did not affect the disinfection kinetics. A chlorine contact tank with the injection chlorine level of 1 mg $Cl_2/L$ and the hydraulic retention time of 1.25 min is estimated to reduce total coliform from $1{\times}10^5MPN/mL$ to 1,000 MPN/mL at 271 mg SS/L, $15^{\circ}C$, and pH 8.0. Chlorine would be a proper option for the disinfection of CSO.
Journal of the Korean Society for Marine Environment & Energy
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v.11
no.2
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pp.70-77
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2008
In this study, to establish countermeasure from marine casualties as a basic study fur long-term prediction of topographical change around Jinudo in the Nakdong river estuary, spatio-temporal topographical change monitoring was carried out. Also, in order to estimate the deposition variations concerning SS (Suspended Solid) flux which moved at St.S1 during neap and spring tide, respectively. From the topographical monitoring, it was found that the annual mean ground level and deposition rate were 141 mm and 0.36 mm/day and all parts except the northern part of Jinudo had the active topographical changes and a tendency to annually deposit. From vertical distribution of SS net fluxes, $SS_{LH}$ (latitudinal SS net flux) during spring tide overall flows average 28 $kg/m^2/hr$ (eastward), and $SS_{LV}$ (longitudinal SS net flux) flows average 11.1 $kg/m^2/hr$ (northward). And, $SS_{LH}$ overall flows average 4.8 $kg/m^2/hr$ (eastward), and $SS_{LV}$ flows average 1.5 $kg/m^2/hr$ (northward) during neap tide similar with spring tide. The depth averaged values of the latitudinal and longitudinal SS net fluxes during spring tide were approximately 6 times higher than those during neap tide. As result of, it was considered that topographical change of southern part of Jinudo was affected by resuspension of bottom sediments due to strong current in bottom layer during flood flow.
Lee, Eun Sol;Oh, Ji Yun;Kim, Yu Jong;Yu, A Mi;Jang, Soo Hui;Cho, Hyun Seok;Kim, Kyung Ho;Lee, Seung Deok;Kim, Kap Sung;Kim, Eun Jung
Journal of Acupuncture Research
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v.30
no.5
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pp.25-39
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2013
Objectives : This study was designed to find out the differences of the acupuncture sensation by type of pharmacopuncture. And furthermore we try to find out whether normal saline(NS) is able to be constituted as an appropriate control group for the Calculus Bovis Fel Ursi Moschus(BUM) pharmacopuncture, mountain ginseng pharmacopuncture and sciatica no. 5 pharmacopuncture. Methods : NS and three type of pharmacopunctures were inserted into $ST_{36}$, and $ST_{37}$ of the subjects. Before and after the treatment, subjects completed a questionnaire rating the intensity of 13 kinds of acupuncture sensation(acupuncture sensation scale, ASS). We compared the subjective acupuncture sensation between the NS and three type of pharmacopunctures. Results : BUM pharmacopuncture showed significantly intense acupuncture sensation comparing other two pharmacopunctures and NS. There was no statistically significant difference among mountain ginseng pharmacopuncture, sciatica no. 5 pharmacopuncture and NS. Conclusions : We found that NS may be able to be an placebo pharmacopuncture for mountain ginseng pharmacopuncture and sciatica no. 5 pharmacopuncture. Additional study is needed for placebo pharmacopuncture of BUM pharmacopuncture.
Large metropolitan areas in the US are distinguished by highly fragmented fiscally independent suburban municipalities and special districts. The suburban fragmentation implies the congregation of similar socio-economic groups escaped from central cities and the disparity of geographical resources among local government jurisdictions. This study examines St.Louis County suburbs as a case study for the implictions of suburban governmental fragmentation and the fiscal disparities across local governmental boundaries by analyzing their relationship with public service provision using police and public school services as examples. The distribution of fiscal resources across political boundaries, the processes which created them, and public service inequalities reproduced from the disparity of fiscal resources reveal the causes and consequences of suburban governmental fragmentation. The central part of suburban fragmentation is the segregation of public goods consumption reproduced from the disparity of local fiscal resources, and it acts as a basic geographical segregating force in the suburban spatial organization.
Backgrounds : Recently, commercial indirect moxibustion has been widely used as a substitute for traditional indirect moxibustion by clinicians due to its convenience in using. But, there has been lack of studies about the similarity of thermal stimulation between commercial indirect moxibustion and traditional indirect moxibustion. Objectives : To demonstrate commercial indirect moxibustion can substitute for traditional indirect moxibustion by comparing thermal stimulation between the two. Methods : We measured bottom temperature of commercial indirect moxibustion and traditional indirect moxibustion with various thicknesses of ginger and garlic. A clinical trial was performed on 18 healthy subjects to evaluate thermal stimualtion. The subjects were randomly assigned into two groups, traditional indirect moxibustion with ginger and garlic. The skin temperature and heating time were measured. They received moxibustion therapy with commercial indirect moxibustion and traditional indirect moxibustion according to their groups. Acupoint Chogcoe($LU_6$), Zusanli($ST_{36}$) and Tianshu($ST_{25}$) were used in each group for 3 times. Results : There was no difference in bottom temperature among commercial indirect moxibustion, traditional indirect moxibustion with 2mm ginger and 3mm garlic. No major difference was detected in the subjects' baseline data. There was no difference in skin temperature and heating time among these 3 type of moxibustions. Also, No major difference was detected in VAS score of thermal stimulation and frequency of burns among these 3 type of moxibustions. But, most subjects regarded their sensation weren't identical because of its own distinct characteristics. Conclusions : Commercial indirect moxibustion, traditional indirect moxibustion with 2mm ginger and 3mm garlic have same thermal stimulation. Thus, commercial indirect moxibustion can substitute for traditional indirect moxibustion.
The main purpose of this study is to differentiate Sasang Constitutions of men and women by measuring 5 parts of the body. By the definitions about Four Chos(四焦) in ${\ulcorner}$The Discourse on Viscera and Bowels(臟腑論)${\lrcorner}$, the body is divided into four parts and sets up the five borderlines. They are Top-line, Bosom-line, Stomach-line, Navel-line and Bottom-line. Top-line(第一線) is the horizontal distance between Rt. & Lt. Coracoid processes passing through the acupuncture point CV-22(天突穴) or the horizontal distance between Rt. & Lt. beginning points of Axillary lines. Bosom-line(第二線) is the horizontal distance passing through Rt.& Lt. Nipples. Stomach-line(第三線) is the horizontal distance passing through Rt.& Lt. acupuncture point ST-19(不容穴). Navel-line(第四線) is the horizontal distance passing through the navel(CV-8; 神闕穴) and the acupuncture point ST-25(天樞穴). Bottom-line(第五線) is the horizontal distance between Rt. & Lt. Anterior Superior Iliac Spine(ASIS). The measured numerical values of Five Lines are the most important factors to classify the Four Constitutions(Taeyangin, Taeumin, Soyangin, Soeumin). Among the five lines, Stomach-line(Middle-line) is the base line to decide Yin and Yang. As a result, I could differentiate the four Constitutions as follows. 1. Taeyangin's Top-line is more than 36cm long. Top-line is the longest. Bottom-line is the shortest. The length-difference between Top-line and Bottom-line is more or less 10cm long. 2. In case of Taeumin, Navel-line is the longest. The numerical values of Middle and Bottom lines are almost the same or Bottom-line is shorter than Middle-line by 1 or 2 cm long. In some special case, the five lines are all the same and the body just looks like a log. 3. In case of Soyangin, just like Taeyangin, Top-line is the longest. Bottom-line is the shortest. The length of Top-line is ranged between 30cm to 35cm long. From Top-line to Bottom-line, the length gradually diminishes each by 2cm: for example, 34-32-30-28-26. 4. In case of Soeumin, the longest one is Bottom-line. Specially, Navel-line is shorter than Middle-line. Bottom-line is longer than the Middle.
Journal of Korean Home Economics Education Association
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v.22
no.1
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pp.97-115
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2010
The purpose of this study was to analyze the frequency, level, and location of Bloom's cognitive domain questions in the middle school home economics(HE) text books applied to the 7th curriculum. Analyzed textbooks were selected 15 textbooks per a grade produced from 5 publishing firms. The result of this study followed: First, the biggest number of questions belonged to Bloom's cognitive domain in HE: text was in 1st grade (36.9%), following 2nd(33.6%) and 3th(29.5%). Most questions the HE textbooks according to Bloom's cognitive domain consisted of those about the understanding(28.9%), application(28.3%) and knowledge(21.8%). Second, in case of the location of questions, the subcategory of Questions after Reading occupied 49.2% of all in Bloom's cognitive questions, the case of Questions During Reading was 36.7%, and the subcategory of Questions Before Reading was 14.1%. The rate of understanding questions was the highest as 43.2% in the Bloom's cognitive domain, and the application questions' rate were 28.4%. Analyzed the questions located in questions, the result showed that application-level questions in textbooks were the highest with 36.7%, Regarding to the questions located after Reading, the knowledge question occupied the highest with 33.4%, and next was the understanding questions(26.8%), following the application questions(21,7%).
The objective of this study was to investigate the effects of abnormal ovarian cycles after superovulation treatment of Hanwoo donors. Thirty six, at random stages of the estrous cycle, received a CIDR. Four days later, the animals were superovulated with a total of 28AU FSH (Antorin, 2AU=1 ml) administered twice daily in constant doses over 4 days. On the 3th administration of FSH, CIDR was withdrawn and 25 mg $PGF_2{\alpha}$ was administered. Cows were artificially inseminated twice after estrous detection at 12 hr intervals. The cows received $100\;{\mu}g$ GnRH at the time of Ind insemination. Embryos were recovered 7 or 8 days after the 1st insemination. The cows were considered to have resumed ovarian cyclicity on the day of ovulation if followed by regular ovarian cycles. 50.0 percentage of the cows (18/36) had normal resumption of ovarian cyclicity (resumption within 40 days after superovulation), and 50.0% (18/36) had delayed resumption(resumption did not occur until>40 days after superovulation). Delayed resumption Type II (first ovulation did not occur until $\geq$ 40 days after superovulation, i.e. delayed first ovulation 33.3%) were the most common types of delayed resumptions. The mean numbers of total ova from < 10 and 10$\leq$ of corpora lutea (CL) was 7.3 and 13.9, respectively. The number of transferable embryos differed between < 10 and 10$\leq$ CL was 4.2 and 5.1, respectively. 11.1 percentage of the cows (4/36) did not resumption their ovarian cyclicity until 60 days after superovulation treatment.
The objective of this study was to examine the impact of changes in maternal age and parity distribution on birth weight. The study population included 7,786 single live births in 1977 and 8,671 single live births in 1987 delivered at 9 medical facilities in Pusan. Data were obtained from the delivery record. The proportion of infants born to the mothers of 25-29 years increased from 56.475 in 1977 to 65.1% in 1987 and the proportion to the mothers of 30-34 years increased from 18.8% in 1977 to 21.6% in 1987. In the same period, the proportions of 1st and 2nd birth order were increased from 56.9% and 28.8% to 59.9% and 36.8%, respectively. The proportion of infants born to the age group of ${\leq}24\;and\;35{\leq}$ years were decreased in 1987. The proportion of births of the third or higher birth order was decreased from 14.2% in 1977 to 3.3% in 1987. Low birth weight (<2500gm) incidence rate was 5.3% in 1977 and it was decreased to 4.0% in 1987. It was estimated that changes in maternal age-parity distribution accounted for 10.7% of the decreased in low birth weight incidence rate. Rest of the change (89.3%) was attributed to the reduction of age-parity specific low birth weight incidence rate. Application of the direct adjustment method was considered to be an adequate tool for evaluating the impact of family planning on neonatal health.
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