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The Role of Protein Kinase C for Prolactin Secretion in Chicken Primary Pituitary Cell Culture (산란계의 뇌하수체 세포배양에서 Prolactin의 생성에 관계하는 Protein Kinase C의 역할)

  • 선상수
    • Korean Journal of Poultry Science
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    • v.23 no.3
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    • pp.113-119
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    • 1996
  • A series of experiments were conducted to investigate the role of protein kinase C (PKC) as a second messenger in vasoactive intestinal peptide (VIP) mediated prolactin secretion. Primary pituitary cells (106 cells/treatment) were separated from laying hens and incubated in M-199 with 5% chicken serum and 5% fetal calf serum. The VIP(0.1 $\pi$M) treatment enhanced prolactin Secretion into media upto 9-fold during 48-h incubation. The phorbol 12-myristate 13-acetate (PMA), a PKG agonist, increased prolactin secretion upto 2-fold at 0.1 nM PMA (P<0.01), and the prolactin secretion was not significantly higher than this concentration. Staurosporine (ST; 1.0$\pi$M) a PKC antagonist, decreased by 70% of 0.1 $\pi$M VIP-stimulated prolactin secretion and by 48% of 10 ${\mu}$M PMA-stimulated prolactin secretion (P<0.01). However, pituitary cell prolactin content did not differ in any treatment (P>0.05). In conclusion, these results indicate that the PKC second messenger system is involved in VIP-stimulated prolactin release in chicken primary pituitary cell culture.

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Blood Protein Polymorphisms of Native Fowls in Laos

  • Okamoto, S.;Tsunekawa, N.;Kawamoto, Y.;Worawut, R.;Kawabe, K.;Maeda, Y.;Nishida, T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.7
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    • pp.1011-1014
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    • 1999
  • Blood protein polymorphism of fowls in Laos was analyzed by electrophoresis. Blood samples were collected in the area of Viangchan, Louangphrabang and Pakxe. Out of 17 loci, polymorphism was detected at the following seven loci; ES-1, Amy-1, Akp-akp, Akp-2, Alb, Tf and Pas. The other ten loci; Amy-3, LDH, 6-PGD, PGM, PHI, To, MDH, Es-D, Hb-l, Hb-2 were noted to be monomorphic. The proportion of polymorphic loci $(P_{poly})$, the expected average heterozygosity per individual ($\bar{H}$), and the subdivision index $(G_{ST})$ of the native fowl in Laos was $0.412{\pm}0.123$, 0.106 and 0.026, respectively. Genetic distance between native fowls in Laos, Bangladesh, and Nepal was clustered in one group.

Exchange Bias in Cr2O3/Fe3O4 Core/Shell Nanoparticles

  • Yun, B.K.;Koo, Y.S.;Jung, J.H.
    • Journal of Magnetics
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    • v.14 no.4
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    • pp.147-149
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    • 2009
  • We report the exchange bias in antiferromagnet/ferrimagnet $Cr_2O_3/Fe_3O_4$ core/shell nanoparticles. The magnetic field hysteresis curve for $Cr_2O_3/Fe_3O_4$ nanoparticles after field-cooling (FC) clearly showed both horizontal ($H_{EB}{\sim}$610 Oe) and vertical (${\Delta}M{\sim}$5.6 emu/g) shifts at 5 K. These shifts disappeared as the temperature increased toward the Neel temperature of $Cr_2O_3\;(T_N{\sim}$307 K). The $H_{EB}\;and\;{\Delta}M$ values were sharply decreased between the $1^{st}\;and\;the\;2^{nd}$ magnetic field cycles, and then slowly decreased with further cycling. These results are discussed in terms of the formation of single domains with pinned, uncompensated, antiferromagnetic spin and their evolution into multi-domains with cycling.

A Study on the Shape Design of Micro Speaker Diaphragm (마이크로 스피커 다이아프램의 형상설계에 관한 연구)

  • Hong, Do-Kwa;Woo, Byung-Chul;Kim, Dong-Young;Ahn, Chan-Woo
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.15 no.7 s.100
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    • pp.775-780
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    • 2005
  • This study uses a characteristic function to explain correlations between the objective function and design variables. Analysis of means and table of orthogonal array were carried out. The change of shape of diaphragm, thickness of diaphragm and voice coil weight based on the table of orthogonal array Is made. Therefore this study carried to decide design variables for minimizing 1st natural frequency and maximizing 2nd natural frequency of diaphragm using design of experiments and characteristic function with constraints. we showed improved design variables.

Subacute toxicities and toxicokinetics of CJ-10882, a type IV phosphodiesterase inhibitor, after 4-week repeated oral administration in dogs

  • Junghee Han;Cha, Shin-Woo;Im, Doo-Hyun;Chung, Moon-Koo
    • Proceedings of the Korean Society of Toxicology Conference
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    • 2003.05a
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    • pp.43-44
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    • 2003
  • The subacute toxicity and toxicokinetics of a type IV phosphodiesterase inhibitor, CJ-10882, were evaluated after single (on the 1st day) and 4-week (on the 27th day) oral administration of the drug, in doses of 0 (to serve as a control), 2, 10 and 50 mg/kg/d, to male and female dogs (n = 3 for male and female dogs for each dose). During the test period, clinical signs, mortality, body weight, food consumption, ophthalmoscopy, urinalysis, hematology, serum biochemistry, gross findings, organ weight and histopathology were examined.(omitted)

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Photo and Electrocatalytic Treatment of Textile Wastewater and Its Comparison

  • Singaravadivel, C.;Vanitha, M.;Balasubramanian, N.
    • Journal of Electrochemical Science and Technology
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    • v.3 no.1
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    • pp.44-49
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    • 2012
  • Electrochemical and photochemical techniques have been proved to be effective for the removal of organic pollutants in textile wastewater. The present study deals with degradation of synthetic textile effluents containing reactive dyes and assisting chemicals, using electro oxidation and photo catalytic treatment. The influence of various operating parameters such as dye concentration, current density, supporting electrolyte concentration and lamp intensity on TOC removal has been determined. From the present investigation it has been observed that nearly 70% of TOC removal has been recorded for electrooxidation treatment with current density 5 mA/$dm^2$, supporting electrolyte concentration of 3 g/L and in photocatalytic treatment with 250 V as optimum lamp intensity nearly 67% of TOC removal was observed. The result indicates that electro oxidation treatment is more efficient than photocatalytic treatment for dye degradation.

Modified Wilkinson Power Divider for Harmonic 제거 (9개의 하모닉을 억제하는 월킨슨 전력 분배기)

  • Kang, In-Ho;Kim, Jung-Hoon
    • Proceedings of the Korea Electromagnetic Engineering Society Conference
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    • 2003.11a
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    • pp.273-277
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    • 2003
  • This paper presents a structure of the Wilkinson power divider that can suppress the 9ea harmonic output. The power divider consists of ${\lambda}/4n$ open stubs, which are located at the $3{\lambda}/4$ branches and parallel connection of resistor which shunts the output ports. Experimental results show that this power divider suppresses from 1st to 9th harmonic components to less than -37dB, while maintaining the characteristics of a conventional Wilkinson power divider; featuring an equal power split, a simultaneous impedance matching at all ports and a good isolation between output ports. these results agree quite well with the simulation results.

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Design of Reflector Type Frequency Doubler for Undesired Harmonic Suppression Using Harmonic Load Pull Simulation Technique

  • Jang, Jae-Woong;Kim, Yong-Hoon
    • Journal of electromagnetic engineering and science
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    • v.7 no.4
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    • pp.175-182
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    • 2007
  • In this paper, a study on the reflector type frequency doubler, to suppress the undesired harmonics, is presented. A 12 to 24 GHz reflective frequency doubler is simulated and experimented. Design procedure of the frequency doubler with reflector is provided and the frequency doubler with good spectral purity is fabricated successfully. It has harmonic suppression of the $40{\sim}50\;dBc$ in the $1^{st}$ harmonic and the $50{\sim}60\;dB$ in the $3^{rd}$ harmonic with no additional filter. And, it has conversion gain with the input power of 0 dBm over bandwidth of 500 MHz. A NEC's ne71300(N) GaAs FET is used and the nonlinear model(EEFET3) using IC-CAP program is extracted for harmonic load pull simulation. Good agreement between simulated and measured results has been achieved.

Structural, FTIR and ac conductivity studies of NaMeO3 (Me ≡ Nb, Ta) ceramics

  • Roy, Sumit K.;Singh, S.N.;Kumar, K.;Prasad, K.
    • Advances in materials Research
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    • v.2 no.3
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    • pp.173-180
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    • 2013
  • Lead-free complex perovskite ceramics $NaMeO_3$ ($Me{\equiv}Nb$, Ta) were synthesized using conventional solid state reaction technique and characterized by structural, FTIR and electrical (dielectric and ac conductivity) studies. The crystal symmetry, space group and unit cell dimensions were determined from the experimental results using FullProf software. XRD analysis of the compound indicated the formation of single-phase orthorhombic structure with the space group Pmmm (47). Dielectric studies showed the diffuse phase transition at $394^{\circ}C$ for $NaNbO_3$ and $430^{\circ}C$ for $NaTaO_3$. Ac conductivity in both the compounds follows Jonscher's power law.

On-line Real Time Soil Sensor

  • Shibusawa, S.
    • Agricultural and Biosystems Engineering
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    • v.4 no.1
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    • pp.28-33
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    • 2003
  • Achievements in the real-time soil spectro-photometer are: an improved soil penetrator to ensure a uniform soil surface under high speed conditions, real-time collecting of underground soil reflectance, getting underground soil color images, use of a RTK-GPS, and all units are arranged for compactness. With the soil spectrophotometer, field experiments were conducted in a 0.5 ha paddy field. With the original reflectance, averaging and multiple scatter correction, Kubelka-Munk (KM) transformation as soil absorption, its 1st and 2nd derivatives were calculated. When the spectra was highly correlated with the soil parameters, stepwise regression analysis was conducted. Results include the best prediction models for moisture, soil organic matter (SOM), nitrate nitrogen (NO$_3$-N), pH and electric conductivity (EC), and soil maps obtained by block kriging analysis.

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