• 제목/요약/키워드: SSF

검색결과 164건 처리시간 0.031초

Construction of a Thermotolerant Saccharomyces cerevisiae Strain for Bioethanol Production with Reduced Fermentation Time and Saccharifying Enzyme Dose

  • Lim, Ji Sung;Jang, You Ri;Lim, Young Hoon;Kim, Keun
    • Journal of Microbiology and Biotechnology
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    • 제22권10호
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    • pp.1401-1405
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    • 2012
  • A thermotolerant Saccharomyces cerevisiae mutant strain, TT6, was constructed after multi-parental hybridization of five mutant strains obtained by UV or NTG treatment of the original strain, S. cerevisiae KV1. When incubated at $40^{\circ}C$ in YPD broth, TT6 began to grow exponentially in 10 h, but KV1 did not show any noticeable growth even after 22 h. The thermotolerant growth of TT6 was confirmed by serial dilution assay at $42^{\circ}C$; TT6 grew at a cell concentration ($10^{-5}$) 10,000 times lower than that of KV1 ($10^{-1}$). Whereas ethanol production from YP containing 23% (w/v) glucose by KV1 decreased with increasing temperature from $30^{\circ}C$ to $36^{\circ}C$, ethanol production by TT6 did not decrease at temperatures up to $37^{\circ}C$. When TT6 was tested for ethanol production at $36^{\circ}C$ by simultaneous saccharification and fermentation (SSF) from 23% corn, 24 h of fermentation time or 50% of the glucoamylase dose was saved when compared with KV1 at $30^{\circ}C$. The ethanol yield from corn by SSF with TT6 at $36^{\circ}C$ was 91.7% of the theoretical yield, whereas that of KV1 at $30^{\circ}C$ was 90.6%.

Effects of Sit-to-stand Training with Various Foot Positions Combined with Visual Feedback on Postural Alignment and Balance in Stroke Patients

  • Kim, Su-Jin;Son, Ho-Hee
    • 대한물리의학회지
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    • 제16권4호
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    • pp.55-65
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    • 2021
  • PURPOSE: This study compared the effects of sit-to-stand training with various foot positions combined with visual feedback on the postural alignment and balance. METHODS: Thirty stroke patients were assigned randomly into three groups of standing with a symmetrical foot position (SSF) (n = 10), asymmetrical foot position with the affected foot at the rear (SAF) (n = 10), and visual feedback and asymmetrical foot position (SVAF) (n = 10). Sit-to-stand training with different foot positions was performed for 30 minutes a day, five times a week, for a total of four weeks, and the effects on postural alignment and balance were assessed. RESULTS: The angle between the midline and scapula peak of the affected side was decreased significantly at sitting and thigh-off in the SAF group and at sitting, thigh-off, and standing in the SVAF group (p < .05). The angle between the midline and scapula peak of the non-affected side was increased significantly at sitting and thigh-off in the SAF group and at sitting, thigh-off, and standing in the SVAF group, the difference in the angle between the scapular peaks of the left and right sides was decreased significantly at sitting and thigh-off in SSF group, and at sitting in SAF group (p < .05). In the SVAF group, the angle at sitting, thigh-off, and standing was decreased significantly (p < .05). A comparison of the balance ability showed that BSS in the SVAF group was improved significantly (p < .05). CONCLUSION: Based on these results, the postural alignment and balance ability were improved in stroke patients who participated in sit-to-stand with visual feedback and asymmetrical foot position training.

비점오염물질 저감을 위한 주암호 생태습지의 수생태학적 효율 평가 (Evaluation of Aquatic Ecological Efficiency in Juam Lake Eco-wetlands for Reducing Non-point Source Pollutants)

  • 이상규;서동철;최익원;강세원;서영진;임병진;이준배;김상돈;허종수;조주식
    • 한국토양비료학회지
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    • 제45권2호
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    • pp.317-324
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    • 2012
  • 비점오염원 저감을 위해 설치된 주암호 생태습지의 수질정화효율을 평가하기 위하여 시스템별 및 시기별 오염물질의 함량과 처리효율을 조사하였다. 유입원수의 BOD, COD, SS, T-N 및 T-P의 함량은 각각 0.20~0.91, 1.24~8.00, 0.6~8.6, 0.04~2.50 및 $0.001{\sim}0.685mg\;L^{-1}$ 범위로 나타났다. 주암호 생태습지 유입수의 대부분이 현행 오염물질 수질기준을 초과하지 않은 상태로 유입되었지만 비점오염원이 많이 유입되는 특정 시기 (장마철)에는 높은 오염물질 함량을 나타내었다. 시기별로는 전반적으로 여름철에 유입원수의 영양염류의 유입이 다른 시기에 비해 높은 경향이었다. 전반적으로 C 시스템이 오염물질의 처리효율이 가장 높은 경향이었다. A 시스템의 습지들은 대부분 겨풀이 우점하고 있었으며 B 시스템의 습지들은 겨풀과 연꽃이 우점하고 있었다. C 시스템의 습지들은 겨풀, 수련 및 부들이 우점하고 있었다. 주암호 생태습지는 오염물질의 처리능이 전반적으로 안정화 상태에 도달하지 못한 것으로 판단되며, 특히 겨풀 및 물 참새피 등 식물교란종의 습지 내 침투로 인하여 습지의 수질정화효율이 전반적으로 낮았다. 따라서 본 연구결과로부터 조성초기 인공습지는 식생의 발달 여부가 수질의 안정화와 습지의 수생태학적 역할을 증대시키는데 중요한 역할을 하는 것으로 판단된다.

바이오에탄올 생산에 적합한 백합나무(Liriodendron tulipifera)의 oxalic acid 전처리 효과 탐색 (Effect of Oxalic Acid Pretreatment on Yellow Poplar (Liriodendron tulipifera) for Ethanol Production)

  • 김혜연;이재원;;곽기섭;최인규
    • Journal of the Korean Wood Science and Technology
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    • 제37권4호
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    • pp.397-405
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    • 2009
  • 본 연구에서는 국내 조림 수종인 백합나무를 바이오에탄올 생산용 자원으로 이용하고자 oxalic acid 전처리 방법을 도입하여 가능성을 타진하였다. $160^{\circ}C$에서 0.037 g/g oxalic acid로 20분 전처리하였을 때 $40.22g/{\ell}$의 발효가능한 당을 생산하였으며, 처리된 고형 바이오매스를 이용하여 동시당화발효를 수행한 결과 72시간 후 $8.6g/{\ell}$의 에탄올을 생산하였다. 같은 조건에서 반응시간을 증가시켜 40분 처리하였을 때 $32.66g/{\ell}$의 발효가능한 당을 생산하였고 동시당화발효로 72시간 후 $9.5g/{\ell}$의 에탄올을 생산하였다. 가수분해산물을 분석한 결과, 같은 조건에서 반응시간이 증가함에 따라 acetic acid, 5-HMF, furfural, total phenols와 같은 발효저해물질이 증가하였다. 이와 같은 발효저해물질은 반응시간보다는 초기 oxalic acid 첨가량에 영향을 받았다. Acetic acid 생산량은 저농도(0.013 g/g)의 oxalic acid를 사용하였을 때 $3.39{\sim}5.78g/{\ell}$로 나타났으며 xylose 분해산물인 furfural은 glucose의 분해산물인 5-HMF보다 2~3배 많게 가수분해산물에 존재하였다. 리그닌 분해산물로 예측되는 total phenols는 모든 조건에서 $5g/{\ell}$ 이상이 검출되었다. 가수분해산물과 동시당화발효로부터 에탄올 생산량을 분석한 결과 0.037 g/g oxalic acid로 20분 전처리한 가수분해산물과 고형 바이오매스로부터 가장 높은 에탄올 생산을 예측할 수 있다.

Optimization of Extraction Parameters for Keratinase Recovery from Fermented Feather under Solid State Fermentation by Streptomyces sp. NRC 13S

  • Shata, Hoda Mohamed Abdel Halim;Farid, Mohamed Abdel Fattah
    • Journal of Applied Biological Chemistry
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    • 제55권3호
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    • pp.149-156
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    • 2012
  • The effects of solvent type and concentration, solid/liquid ratio, extraction time and repeated extraction on recovery of keratinase from solid-state fermentation (SSF) of chicken feather by a local Streptomyces sp. NRC 13S were investigated in order to establish the experimental conditions for keratinase yield. Among solvents tested, 0.5% (v/v) glycerol was the best. Box-Behnken design was used to investigate the effect of relevant variables on keratinase recovery. The factors investigated were solid/liquid ratio (1:1.66-1:6.66 g/mL), glycerol concentration (0.5-5% v/v) and repeated extraction (1-5 cycle). The results showed that the maximum recovery of keratinase (6933.3 U/gfs) was obtained using 0.5 (v/v) glycerol as extracting solvent, in a solid/liquid ratio of 1:5 and three extraction cycles.

IEEE 802.11 WLANs 환경에서 핫스팟의 혼잡을 분산하는 AP 선택정책 (A Novel Access Point Selection Policy for Load Balancing in IEEE 802.11 WLANs)

  • 이광교;최창열
    • 산업기술연구
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    • 제28권A호
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    • pp.105-111
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    • 2008
  • A typical AP (Access Point) selection policy is to select an AP based on the received signal strength indicator (RSSI), ignoring its load. If multiple stations are deployed densely at a particular area, a typical AP selection policy will bring about the overall network throughput degradation. This paper proposes a novel AP selection policy taking into consideration not only signal strength of the APs but also AP loads to avoid Hotspot congestion. An experiment on Alinker implementing proposed AP selection policy, demonstrates that the proposed policy achieves close to optimal load balancing and grants the maximum transmission rate to stations in comparison with SSF (Strongest-Signal-First) and LLF (Least-Loaded-First).

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Xylanase Production in Solid State Fermentation by Aspergillus niger Mutant using Statistical Experimental Designs

  • 박양순;강성우;이진석;김승욱
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2001년도 추계학술발표대회
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    • pp.337-340
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    • 2001
  • The production of xylanase from Aspergillus niger mutant in SSF was optimized by' using statistical experimental designs. An inoculum size of $5{\times}10^5$ spores/g. initial moisture content of 65 %. cultivation time of 5 days and 10 times concentration of basal medium containing 50 times concentration of CSL were optimum for xylanase production ‘ Under the optimized conditions. the activity and productivity of xytanase obtained after 5 days of fermentation were 5.071 IU/gram of rice straw and 14.790 IU/l.h. respectively.

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공작기계의 고장원인을 효과적으로 생성하기 위한 Switching Function Generator 개발 (Development of Switching Function Generator for Fault Reasons of CNC Machine Tool)

  • 김동훈;김도연;김선호;이은애;한기상
    • 한국공작기계학회:학술대회논문집
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    • 한국공작기계학회 2002년도 춘계학술대회 논문집
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    • pp.48-52
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    • 2002
  • The fault analysis of the CNC machine tool which is controlled sequentially by PLC is generally based on ladder diagram. When machine tool has faults, it takes a lot of operator's experiences and times to identify logical relationship because ladder diagram is a step structured language. Therefore the technologies of finding out fault reasons automatically is necessary. In this paper, the SFG(Switching Function Generator) system is developed to analysis fault reasons correctly. The SF(Switching Function) and SSF(Step Switching Function) generated from SFG based on ladder diagram are experimented to identify the performance of SFG.

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차량무게중심의 측정 및 추정에 관한 연구 (A Experimental Study on the Measurement and Estimation of Vehicle Center of Gravity)

  • 이명수;김상섭
    • 한국자동차공학회논문집
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    • 제18권5호
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    • pp.91-99
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    • 2010
  • The center of gravity on vehicle is a fundamentally important point for assessing and measuring the characteristics of vehicle dynamics. Especially, the center of gravity height on vehicles is the closest factor with respect to rollover accidents in a social issue nowadays. In this paper, the center of gravity height in conjunction with vehicle parameters of vehicle weight, driving axle and roof height after measured by vehicle weight and loading location by means of VCGM developed by KATRI with good performance that the accuracy was less than 0.6% and repeatability 0.3% for vehicles being used in the whole world was observed. As a result of study, the location of center of gravity height on vehicle was able to be estimated with only roof height on vehicle.

난류선회 등온 및 분무연소 유동에 대한 수치해석 (Numerical Study of Turbulent Swirling Isothermal and Spray-Combusting Flows)

  • 김성구;안국영;김용모;이창식
    • 한국자동차공학회논문집
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    • 제4권5호
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    • pp.130-141
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    • 1996
  • Numerical study of a confined, swirling, isothermal and spray-combusting flows has been presented. The pressure-velocity coupling in the Eulerian gas-phase equation is handled by the improved PISO algorithm. The droplet dispersion by turbulence is introduced by a Stochastic Separated Flow(SSF) model. The k-$\varepsilon$ turbulence model and the eddy dissipation model are employed to account for turbulence-combustion interaction. The detailed comparison with experimental data has been made for the isothermal jet swirling flows and the nearly monodisperse spray-combusting flow in the swirl combustor.

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