• Title/Summary/Keyword: SSC2

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A post-genome-wide association study validating the association of the glycophorin C gene with serum hemoglobin level in pig

  • Liu, Yang;Hu, Zhengzheng;Yang, Chen;Wang, Shiwei;Wang, Wenwen;Zhang, Qin
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.5
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    • pp.638-642
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    • 2017
  • Objective: This study aimed to validate the statistical evidence from the genome-wide association study (GWAS) as true-positive and to better understand the effects of the glycophorin C (GYPC) gene on serum hemoglobin traits. Methods: Our initial GWAS revealed the presence of two single nucleotide polymorphisms (SNPs) (ASGA0069038 and ALGA0084612) for the hemoglobin concentration trait (HGB) in the 2.48 Mb region of SSC15. From this target region, GYPC was selected as a promising gene that associated with serum HGB traits in pigs. SNPs within the GYPC gene were detected by sequencing. Thereafter, we performed association analysis of the variant with the serum hemoglobin level in three pig populations. Results: We identified one SNP (g.29625094 T>C) in exon 3 of the GYPC gene. Statistical analysis showed a significant association of the SNP with the serum hemoglobin level on day 20 (p<0.05). By quantitative real-time polymerase chain reaction, the GYPC gene was expressed in eight different tissues. Conclusion: These results might improve our understanding of GYPC function and provide evidence for its association with serum hemoglobin traits in the pig. These results also indicate that the GYPC gene might serve as a useful marker in pig breeding programs.

Characteristics of Leaves, Roots, and Fruit as Influenced by Energized-Functional Water Supply in Fuji Apple Trees (Energized 기능수 처리에 따른 후지사과의 잎, 뿌리 및 과실특성)

  • Kim, Wol Soo;Chung, Soon Ju
    • Horticultural Science & Technology
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    • v.16 no.2
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    • pp.233-235
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    • 1998
  • Energized-functional water (EFW) and powder (EFP) were manufactured by Kyungwon Institute of Life Science, Seoul, through a series of processes; tap water ultra-purification energy imprinting with catalysts in platinum columns mixing energy-imprinted water + activated zeolites + photosynthetic bacteria fermenting at $25^{\circ}C$ filtering EFW and/or EFP. A single application of EFP to soil under tree canopy before bud burst, combined with three EFW applications to soil during growth of 'Fuji' apples (Malus domestica Borkh.) resulted in a higher Ca concentrations in fruit skins and flesh, and lower Ca and N concentrations in leaves and shoot-bark tissues. EFW also stimulated the net photosynthesis of leaves and root activity. Soluble solid concentrations (SSC) and anthocyanin levels of fruits were also significantly increased at harvest, producing greater firmness and less core browning during storage at $0^{\circ}C$. However, there was no significant difference in titratable acidity of fruit juice between the EFW treatment and the controls.

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Risk Analysis on Inundation of SOC Facilities under Climate Change (기후변화에 따른 SOC구조물의 외부/내부침수 위험도 분석)

  • Kim, Beom Jin;Keum, Ho Jun;Lee, Jae Yeong;Kim, Hyun Il;Han, Kun Yeun
    • Proceedings of the Korea Water Resources Association Conference
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    • 2019.05a
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    • pp.52-52
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    • 2019
  • 최근 국가시설물에서는 2000년대 이후 집중호우 등으로 인한 대상 부지 내의 홍수 발생 시 주요시설물에 기능 마비가 발생할 수 있고, 궁극적으로는 대규모 사고로 이어질 수 있기 때문에 외부침수에 대비할 수 있는 위험도 분석이 필요하다. 대상 부지에서의 외부침수의 원인으로서는 LIP(Local Intensive Precipitation)에 의한 홍수 발생조건, 인근에 댐, 제방 등이 위치한 경우 이들 시설물의 붕괴에 따른 홍수류의 원전 유입, 지진해일/폭풍해일에 의한 바다로부터의 홍수 유입 등이 대표적인 예이다. 따라서 대상 부지 및 그 SOC시설물의 안전도를 높은 수준에서 관리하기 위해서는 극한홍수가 유입될 때 침수심, 침수유속, 침수시간, 침수강도 등의 재해도를 분석하여야하고, 이들 SOC시설물의 취약도 평가를 실시하고 재해도와 취약도를 결합한 연계분석을 통하여 위험도를 재평가하여야 한다. 본 연구에서는 기존 기후변화를 고려한 외부침수 위험도 분석 결과를 바탕으로 대상 부지 내의 내부침수 위험도 분석을 실시하였다. 위험도 분석을 실시하기 위해 현장답사를 통해 물이 외부에서 내부로 유입 가능한 침수패스 경로를 파악하고, 출입문 위치와 창문의 높이, 출입문의 틈간격 및 높이를 파악하였다. 현장답사를 토대로 침수구역을 선정하였다. 침수구역 선정시 대침수구역과 소침수구역 중요기기들이 위치한 구역을 바탕으로 선정하였고, 이를 바탕으로 2차원 침수 해석을 실시하여 각 구역별로 시간대별 침수가능 높이를 산정하였다. 또한 각 구역별 중요기기의 임계높이를 산정하고, 이를 분석된 최대 침수심과 비교하여 각 구역별 침수에 취약한 구역을 산정하였다. 본 연구결과의 바탕으로 사회기반 시설에 대한 보호 및 홍수피해 예방으로 인한 사회비용 절감이 가능하고, 주요시설물의 SSC별 방재대책을 수립하고, 단계별 저감대책을 제시하여 위험도 경감을 위한 대비책을 마련이 가능할 것 이라고 판단된다.

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Characteristics of Growth, Yield and Disease/Pest Occurrence among Major Strawberry Cultivars for Organic Forcing and Semi-forcing Culture (딸기 유기 재배시 주요 품종간 생육, 수량 및 병해충 발생 특성)

  • Kim, Dae-Young;Ko, Kwan-Dal;Yun, Hyung-Kweon;Yoon, Moo-Kyung;Kwak, Jung-Ho;Kim, Tae-Il
    • Journal of Bio-Environment Control
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    • v.17 no.4
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    • pp.336-341
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    • 2008
  • The objective of this research was to investigate the suitable cultivar for organic strawberry (Fragaria ananassa Duch.) cultivation in Korea. The six strawberry cultivars which were bred and commonly cultivated in Korea as forcing and semi-forcing culture were tested in this experiment. To achieve the objectives, various characteristics of these cultivars were investigated for two consecutive years such as growth habit, yield, fruit quality and occurrence rate of disease/pest. As results of this research, the total yield of 'Seolhyang' was the highest among cultivars tested in both forcing and semi-forcing cultivation. The average of soluble solid contents (SSC) in 'Seolhyang' was $12.0^{\circ}Bx$ that is lower than other cultivars and especially below $10^{\circ}Bx$ after March (high temperature period). But there was not much difference in SSC among 6 cultivars until the end of February. The average of hardness in 'Seolhyang' was 279g/${\phi}5\;mm$ which was a little higher than that of 'Akihime'. Titration acidity (TA) in 'Maehyang' was 0.69% which was the highest value among 6 cultivars. Total fresh/root weight of 'Seonhong' and leaf area of 'Seolhyang' were significant among other cultivars' ones in forcing culture. Growth habits (fresh weight, root weight, leaf area, etc.) showed no significant difference among cultivars in semi-forcing culture. The lowest occurrence rate for powdery mildew and aphids was found in 'Seolhyang', but gray mold was severer than other cultivars. The density of two spider mites was the lowest in 'Redpearl'. We have concluded that 'Seolhyang' is the most suitable cultivar for organic strawberry cultivation by the result of yield, fruit quality and resistance of disease/pest.

Effects of 1-methylcyclopropene (1-MCP) treatment on the maintenance of fruit quality of RubyS apples during cold storage ('루비에스' 사과의 저온저장 중 과실품질 유지를 위한 1-methylcyclopropene 처리 효과)

  • Jingi Yoo;Hwajong Yoo;Nay Myo Win;Hee-Young Jung;Young-Je Cho;In-Kyu Kang
    • Food Science and Preservation
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    • v.30 no.1
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    • pp.78-87
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    • 2023
  • This study was conducted to evaluate the effect of different 1-methylcyclopropene (1-MCP) concentrations on fruit quality of small-sized RubyS apples during cold storage. After harvesting, the fruits were treated with 1-MCP at 0.5 or 1 µL/L concentrations and, subsequently, stored at 0℃ for 6 months. After 6 months, the flesh firmness of untreated fruits, which was 85.4 N at harvest, had gradually decreased to 46.5 N; however, that of 1-MCP-treated fruits was maintained at 59.1 and 59.5 N. Titratable acidity (TA) of untreated fruits had also decreased from 0.42 to 0.24%, whereas that of 1-MCP-treated fruits was maintained at 0.26 and 0.27%. Soluble solids content (SSC) did not differ in all fruits. However, the 1-MCP-treated fruits had lower levels of SSC/TA ratio than untreated fruits. Moreover, after 6 months, the ethylene production had increased to 47.0 µL/kg/h in the untreated fruits, whereas 1-MCP blocked the ethylene production at 1.4 and 1.7 µL/kg/h. The weight loss and peel color variables remained unaffected by 1-MCP treatments. Thus, these results indicated that, for RubyS apples, the storability was only 2 months at 0℃ without treatment, which can be extended to 6 months with 1-MCP treatments. The application of 1-MCP at 0.5 µL/L concentration is effectively and economically sufficient to maintain the quality of RubyS apples.

Ceriporia sp. ZLY-2010 in Biodegradation of Polychlorinated Biphenyls : Extracellular Enzymes Production and Effects of Cytochrome P450 Monooxygenase (Ceriporia sp. ZLY-2010에 의한 폴리염화비페닐류의 생분해 : 균체 외 효소활성 및 cytochrome P450 monooxygenase 영향)

  • Hong, Chang-Young;Gwak, Ki-Seob;Lee, Su-Yeon;Kim, Seon-Hong;Jeong, Han-Seob;Choi, In-Gyu
    • Journal of the Korean Wood Science and Technology
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    • v.39 no.6
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    • pp.469-480
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    • 2011
  • In this study, to determine the ligninase activity related to the PCBs degradation of Ceriporia sp. ZLY-2010, the protein contents and manganese peroxidase (MnP) and laccase activities during cultivation on shallow stationary culture (SSC) medium were observed. 4 PCB congeners were selected depending on the number of chlorine substituted on biphenyl. Furthermore, to examine the effects of cytochrome P450 monooxygenase, the inhibition of cytochrome P450 monooxygenase was evaluated by measuring the biodegradation rate when inhibitor such as 1-aminobenzotriazole was added. The extracellular protein contents were affected by PCB congeners in culture media. The total protein in the culture medium showed the biggest differences between the samples containing 2,2',4,4',5,5'-hexachlorobiphenyl and the control. On the other hand, MnP and laccase activity showed dominant increases within samples containing 4,4'-dichlorobiphenyl and 2,3',4',5-tetrachlorobiphenyl. Cytochrome P450 monooxygenase was inhibited by adding inhibitor, 1-aminobenzotriazole in low concentration. Only 2.73% of 2,3',4',5-tetrachlorobiphenyl was degraed on day 1, and biodegradation of 2,2',4,4',5,5'-hexachlorobiphenyl was inhibited as well, showing about 20% of biodegradation rate.

Quality changes of fresh-cut winter squash treated with different postharvest ripening periods and packaging methods (신선편이 단호박 원료의 후숙기간 및 가공 후 포장방법에 따른 저장 중 품질변화)

  • Kim, Ji-Gang;Choi, Ji-Woen;Cho, Mi-Ae
    • Food Science and Preservation
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    • v.21 no.1
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    • pp.17-24
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    • 2014
  • This study was conducted in order to evaluate the effect of postharvest ripening periods and packaging methods on maintaining the quality of fresh-cut winter squash. Winter squash (var. Bouchang) was ripened at $22^{\circ}C$ for 1 or 2 weeks after harvest. The samples were washed in tap water, sanitized in $100{\mu}L/L$ chlorine water, peeled, and cut into 16 parts. Samples were then vacuum packaged or non-vacuum packaged in $80{\mu}m$ nylon/polyethylene (Ny/PE) films and stored at $5^{\circ}C$ for 21 days. Results indicated that different postharvest ripening periods affected gas concentration, firmness, off-odor development, color, and overall quality of fresh-cut winter squash. Samples treated with 2-week ripening periods maintained quality with higher redness value and soluble solid content (SSC) and lower $CO_2$ concentration and off-odor development compared to samples treated with a 1-week ripening period. Non vacuum packaging was effective in increasing visual quality and reducing off-odor development. A combination treatment of 2-week ripening periods and non-vacuum packaging maintained good quality with the lowest off-odor development and the highest visual quality scores at the end of the storage period.

Suppression of Chilling Injury and Maintenance of Quality Characteristics in Prunus Mume Fruits Stored under Controlled Atmosphere (CA 저장에 의한 청매실의 저온장해 발생 억제 및 품질 특성 유지 효과)

  • Choi, Sun-Young;Cho, Mi-Ae;Hong, Yoon-Pyo;Hwang, In-Kyeong;Chung, Dae-Sung;Yun, Seok-Kyu
    • Food Science and Preservation
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    • v.18 no.2
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    • pp.143-148
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    • 2011
  • This study was conducted to investigate the effect of CA(4% $O_2$ and 7.5% $CO_2$) storage on the quality characteristics and chilling injury in 'Nanko' prunus mume fruits at 1, 5, and $9^{\circ}C$. CA storage reduced production of $CO_2$ and $C_2H_4$ significantly. Hue values of fruit skin were significantly higher in fruits stored at $1^{\circ}C$ and $5^{\circ}C$ than $9^{\circ}C$. Weight loss was much lower in fruits stored under CA storage. Soluble solids content (SSC) titratable acids (TA), and firmness were maintained and electrolyte leakage was lower in fruits stored under CA storage. Ratios of chilling injury and decay were increased faster at $5^{\circ}C$ and $9^{\circ}C$ than $1^{\circ}C$. The chilling injury was suppressed in fruits of CA storage compared with control fruits during cold storage. These results indicate that CA storage at $1^{\circ}C$ of prunus mume fruits extended the storage life up to 30 days without quality deterioration. effectively.

Identification of Quantitative Trait Loci(QTL) for Meat Color Trait on Chromosome 7 in Pig (돼지 7번 염색체에서 육색 연관 QTL 확인)

  • Choi, B.H.;Lee, H.Y.;Kim, T.H.;Hong, K.C.;Cheong, I.C.
    • Journal of Animal Science and Technology
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    • v.46 no.4
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    • pp.525-536
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    • 2004
  • The objective of this study was to identify the quantitative traits loci(QTL) for meat quality traits in pigs. Three-generation resource population was constructed from a cross between Korean native boars and Landrace sows. The resource population including founders, $F_1$ and $F_2$ was genotyped for 23 microsatellite markers on chromosome 7. The sex average total length of linkage map on chromosome 7 was estimated 154.6 cM. Meat quality traits including meat pH, meat color, drip loss, shear force, heating loss, crude fat, crude protein, crude ash and water content in muscle were collected from $F_2$ animals. For the QTL mapping, we used $F_2$ QTL Analysis Servlet of QTL express for web-based QTL mapping tools(http://qtl.cap.ed.ac.uk/). The QTLs for CIE-a and CIE-b on SSC7 were significantly detected at 1% and 5% chromosome-wide level, respectively.

Quality of 'Fuji' Apple Packed with Functional MA Film to the Films Thickness (두께별 기능성 MA 필름으로 포장하여 저장한 사과의 품질)

  • Hong, Sang Tai;Park, HyungWoo;Park, Yang-Kyun
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.25 no.2
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    • pp.51-56
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    • 2019
  • The quality of 'Fuji' apple was analyzed the film thickness conditions and packed with functional MA film during 24 weeks storage at $0^{\circ}C$ to weight loss, hardness, acidity and SSC, decay ratio, and vitamin C. The weight loss of apple packed functional MA film was higher than that of contro.Value of the weight loss of apple packed in the control and functional MA filmswere 8.2% and 0.4-0.5%, respectively. The change of hardness and titratable acidity was also lower than that of control. The decay ratio of apple packed with functional MA films were 2.4~4.7%, but that of control was 6.8%. The decay ratio of apple was the lowest at 0.025mm functional MA film. Changes of vitamin C also was lower than that of control. Based on the results, we suggested that the functional MA film with 0.025mm thickness is desirable for keeping the quality of the 'Fuji' apple produced at Geochang, Korea.