• Steel and Composite Structures
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• v.51 no.3
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• pp.289-304
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• 2024

The seismic performance of traditional steel frame-shear wall structures was significantly improved by the application of self-centering steel-reinforced concrete (SRC) wall-panel structures in the steel frames. This novel resilience functionality can rapidly restore the structure after an earthquake. The presented steel frame with steel-reinforced concrete self-centering wall-panel structures (SF-SCW) was validated, indicating its excellent seismic performance. The seismic design method based on bear capacity cannot correctly predict the elastic-plastic performance of the structure, especially certain weak floors that might be caused by a major fracture. A four-level seismic performance index, including intact function, continued utilization, life safety, and near-collapse, was established to achieve the ideal failure mode. The seismic design method, based on structural displacement, was proposed by considering performance objectives of the different seismic action levels. The pushover analysis of a six-floor SF-SCW structure was carried out under the proposed design method and the results showed that this six-floor structure could achieve the predicted failure mode.

• Animal cells and systems
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• v.1 no.3
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• pp.521-526
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• 1997

We isolated a mouse nck cDNA from the thymus cDNA expression library. The cDNA encodes a 377 amino acid protein and displays 97% amino acid sequence identity to human oncogenic protein nck, which is composed almost exclusivelv of three src homology 3 (SH3) domains and one SH2 domain. The sequence analysis also showed that the isolated cDNA is the mouse counterpart of the human nck and different from the mouse grb4, which has been reported to be highly similar to the human nck and, therefore considered as a mouse nck, Northern blot analysis showed that the transcript of the gene was 1.8 kb and was highly expressed in the testis, thymus, and brain but moderately in the liver and lymph node. Western blot analysis showed that the size of the protein was about 47 kDa. Overexpression of the mouse Nck transformed a mouse fibroblast cell line, NIH3T3. The results clearly indicate that normal nck gene has transforming ability and provide an argument against a suggested possibility that the transforming ability of the human nck gene is due to a mutation(s) in the gene.

• Animal cells and systems
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• v.3 no.3
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• pp.331-336
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• 1999

p56$^{Ick}$, a cytoplasmic protein tyrosine kinase of the src family, is non-covalently associated with the cell surface coreceptors CD4 and CD8, which are expressed on thymocytes and mature T cells. The coreceptor protein plays an important role during the differentiation of thymocytes and the activation of T cells. DNA constructs were designed to study the roles of CD4 and CD8 during the differentiation of thymocytes. One is a chimeric cDNA which consists of coding regions for the extracellular domain of CD8a and the transmembrane and cytoplasmic domain of CD4. The other is the same chimeric cDNA but with a point mutation converting Cys to Ala in the Ick-binding site to disrupt the association. We confirmed that the CD8a/CD4 chimeric molecule bound to Ick more efficiently than the wild type CD8a protein. However, the chimeric protein with the Cys$leftrightarro$Ala mutation did not associate with Ick. The results suggest a possibility that the CD8a/CD4 chimeric protein may behave like a CD4 protein in associating with Ick and that it may deliver a signal inside the cell in a similar manner, Analysing effects of the mutant CD8a/CD4 chimeric protein expression in developing thymocytes will elucidate the role of Ick during the determination of CD4/CD8 cell lineages.

• Journal of Korean Society of Steel Construction
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• v.25 no.1
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• pp.47-59
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• 2013

This study that is a successive secondary study right after the primary bending strength test of a new form of U-shape hybrid composite beam is a cyclic seismic test of U-shape hybrid composite beam and column conncetion. Three specimens are built for the variables which are kinds of columns, depth of beam, continuity or discontinuity of upper plate of beam, and a number of steel bars of end-beam. Kinds of columns are a reinforcement concrete column and a ACT column of CFT shape, and beam depth are 300, and 500 mm. Detail of connection is bolt connection with using a short bracket that is commonly use. As the result, deformability of 2~4% is ensured the floor displacement angle. If it is the negative moment, the maximum moment shows that its capacity is above the nominal moment.

• Steel and Composite Structures
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• v.21 no.2
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• pp.289-302
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• 2016

A new kind of partially precast or prefabricated castellated steel reinforced concrete beam, which is abbreviated here as CPSRC beam, was presented and introduced in this paper. This kind of CPSRC beam is composed of a precast outer-part and a cast-in-place inner-part. The precast outer-part is composed of an encased castellated steel shape, reinforcement bars and high performance concrete. The cast-in-place inner-part is made of common strength concrete, and is casted with the floor slabs simultaneously. In order to investigate the shear performance of the CPSRC beam, experiments of six CPSRC T-beam specimens, together with experiments of one cast-in-place SRC control T-beam specimen were conducted. All the specimens were subjected to sagging bending moment (or positive moment). In the tests, the influence of casting different strength of concrete in the cross section on the shear performance of the PPSRC beam was firstly emphasized, and the effect of the shear span-to-depth ratio on that were also especially taken into account too. During the tests, the shear force-deflection curves were recorded, while the strains of concrete, the steel shapes as well as the reinforcement stirrups at the shear zone of the specimens were also measured, and the crack propagation pattern together with the failure pattern was as well observed in detail. Based on the test results, the shear failure mechanism was clearly revealed, and the effect of the concrete strength and shear span-to-depth ratios were investigated. The shear capacity of such kind of CPSRC was furthermore discussed, and the influences of the holes on the steel shape on the shear performance were particularly analyzed.

• Fire Science and Engineering
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• v.26 no.1
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• pp.80-88
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• 2012

This study evaluates the fire resisting capacity of the beam of the legal fire resistance construction, which establishes the Article 3 of the Regulations on Escape and Fire Resistance of Buildings. There are a total of five structures that we consider as legal fire resistance constructions, however, this study has a primary target of the reinforced concrete beam, and tests the fire-resistant performance depend on the covering depth of reinforce concrete. The results showed that it meets the three hours, the maximum statutory fire resistance time, if it was a load ratio of 0.5 and covering depth of 40 cm. Steel framed mortar beam is legal fire resistance structure that it was possessed three hours fire resistance performance, if it was a load ratio of 0.4 and covering depth of 60 mm.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.8 no.2
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• pp.483-503
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• 2014

In this paper, human action recognition using pyramid histograms of oriented gradients and collaborative multi-task learning is proposed. First, we accumulate global activities and construct motion history image (MHI) for both RGB and depth channels respectively to encode the dynamics of one action in different modalities, and then different action descriptors are extracted from depth and RGB MHI to represent global textual and structural characteristics of these actions. Specially, average value in hierarchical block, GIST and pyramid histograms of oriented gradients descriptors are employed to represent human motion. To demonstrate the superiority of the proposed method, we evaluate them by KNN, SVM with linear and RBF kernels, SRC and CRC models on DHA dataset, the well-known dataset for human action recognition. Large scale experimental results show our descriptors are robust, stable and efficient, and outperform the state-of-the-art methods. In addition, we investigate the performance of our descriptors further by combining these descriptors on DHA dataset, and observe that the performances of combined descriptors are much better than just using only sole descriptor. With multimodal features, we also propose a collaborative multi-task learning method for model learning and inference based on transfer learning theory. The main contributions lie in four aspects: 1) the proposed encoding the scheme can filter the stationary part of human body and reduce noise interference; 2) different kind of features and models are assessed, and the neighbor gradients information and pyramid layers are very helpful for representing these actions; 3) The proposed model can fuse the features from different modalities regardless of the sensor types, the ranges of the value, and the dimensions of different features; 4) The latent common knowledge among different modalities can be discovered by transfer learning to boost the performance.

• Molecules and Cells
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• v.43 no.11
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• pp.921-934
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• 2020

Lck-interacting transmembrane adaptor 1 (LIME) has been previously identified as a raft-associated transmembrane protein expressed predominantly in T and B lymphocytes. Although LIME is shown to transduce the immunoreceptor signaling and immunological synapse formation via its tyrosine phosphorylation by Lck, a Src-family kinase, the in vivo function of LIME has remained elusive in the previous studies. Here we report that LIME is preferentially expressed in effector T cells and mediates chemokine-mediated T cell migration. Interestingly, in LIME^-/- mice, while T cell receptor stimulation-dependent proliferation, differentiation to effector T cells, cytotoxic T lymphocyte (CTL) function and regulatory T lymphocyte (Treg) function were normal, only T cell-mediated inflammatory response was significantly defective. The reduced inflammation was accompanied by the impaired infiltration of leukocytes and T cells to the inflammatory sites of LIME^-/- mice. More specifically, the absence of LIME in effector T cells resulted in the reduced migration and defective morphological polarization in response to inflammatory chemokines such as CCL5 and CXCL10. Consistently, LIME^-/- effector T cells were found to be defective in chemokine-mediated activation of Rac1 and Rap1, and dysregulated phosphorylation of Pyk2 and Cas. Taken together, the present findings show that LIME is a critical regulator of inflammatory chemokine-mediated signaling and the subsequent migration of effector T cells to inflammatory sites.

• Korean Journal of Oriental Medicine
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• v.13 no.2 s.20
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• pp.157-164
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• 2007

An imbalance in bone remodeling that is caused by increased bone resorption over bone formation leads to most adult skeletal diseases including osteoporosis. Since the development of anti-resorptive agents from natural substances has recently gained more interest in the treatment of osteoporosis, we evaluated the effects of 222 natural compounds on receptor activator of $NF-{\kappa}B$ ligand (RANKL)-induced of tartrate-resistance acid phosphatase (TRAP) activity in RAW264.7 murine macrophage cell, and found that berberine chloride is one of compounds inhibiting RANKL-induced TRAP activity. Berberine chloride significantly inhibited the RANKL-induced TRAP activity and the formation of multinucleated osteoclasts in a dose-dependent manner. In addition, berberine chloride prevented the RANKL-induced mRNA expression of TRAP, matrix metalloproteinase 9 and c-Src, which have been known to be highly expressed in the process of osteoclastogenesis. Interestingly, berberine chloride prevented the RANKL-induced activation of extracellular signal-regulated kinase (ERK) which is one of mitogen-activated protein (MAP) kinases. In conclusion, berberine chloride could inhibit the osteoclastogenesis via preventing the activation of ERK/MAP kinase signaling pathway.

• Explosives and Blasting
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• v.23 no.3
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• pp.83-89
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• 2005

It follows in deterioration of the steel frame structure and becomes remodeling and removal. The construction work characteristic, economical efficiency and stability environment characteristic are planned and considered hereafter control plan of the steel frame structure which is deteriorated currently to cutting mettled plentifully sued on gas cutting of H beam. However it will not be able to apply from the explosives demolition which is makes a weak instantaneously and then collapses the building at the time. In this study, shape charge was used for cutting of the H-beam. That is the element testing to estimate explosives demolition for steel frame structure. As a result, it is found for single-side rutting method, both-sides rutting methods by H-beam thickness and pre-rutting process. It confirmed an affix method and an ease characteristic by fixing tool. Also, it is shown that air blasting decreased about 8dB(A) in order to reduce air blasting used by sand box. However, it will be required to reduce air blasting little more because explosives demolition will be done in urban site.