Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Overexpression of Mouse Nck Transforms Mouse Febroblast NIH3T3

  • Kim, Young H. (Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University) ;
  • Han, Sun-Mi (Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University) ;
  • Kim, Moon G. (Laboratory of Cellular and Molecular Immunology, NIAID, NIH) ;
  • Park, Dong-Eun (Department of Life Sciences, Kwang-Ju Institute of Science and Technology) ;
  • Park, Sang D. (Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University) ;
  • Seong, Rho H. (Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University)
  • Published : 1997.09.01
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Abstract

We isolated a mouse nck cDNA from the thymus cDNA expression library. The cDNA encodes a 377 amino acid protein and displays 97% amino acid sequence identity to human oncogenic protein nck, which is composed almost exclusivelv of three src homology 3 (SH3) domains and one SH2 domain. The sequence analysis also showed that the isolated cDNA is the mouse counterpart of the human nck and different from the mouse grb4, which has been reported to be highly similar to the human nck and, therefore considered as a mouse nck, Northern blot analysis showed that the transcript of the gene was 1.8 kb and was highly expressed in the testis, thymus, and brain but moderately in the liver and lymph node. Western blot analysis showed that the size of the protein was about 47 kDa. Overexpression of the mouse Nck transformed a mouse fibroblast cell line, NIH3T3. The results clearly indicate that normal nck gene has transforming ability and provide an argument against a suggested possibility that the transforming ability of the human nck gene is due to a mutation(s) in the gene.

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References

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