• 대한수의학회지
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• 제26권2호
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• pp.321-327
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• 1986

The anthelmintic efficacy of Albendazo1e and Ivermectin against gastrointestinal nematodes, trematodes and cestode was tested in naturally infected Korean native goats. Albendazole was medicated at a dose rate of 10mg/kg of body weight orally and Ivermectin was injected at a dose rate of 0.2mg/kg of body weight subcutaneously. The efficacy was measured by faecal examinations on the day 7th and the day 14th :after treatment. The results obtained were summarized as follows : 1. The efficacy of Albendazole against trematodes and cestode was shown 91.7% in Fasciola hepatica, 68.8% in Paramphislomum spp., 66.7% in Eurytrema pancreaticum and 100% in Moniezia expansa(cestode). 2. The efficacy of Ivermectin against trematodes and cestode was shown 38.8% in Fasciola hepalica, 26.1% in Paramphistomum spp., 22,2% in Eurytrema pancreaticum and 100% in Moniezia expansa(cestode). 3. The anthelmintic efficacy of Albendazole against gastrointestinal nematodes was shown 97.7% in Haemonchus contorus and 100% in Oesophagostomum spp., Ostertagia spp., Bunostomum trigonocephalum, Trichostrongylus spp., Strongyloides papillosus and Cooperia spp. 4. The efficacy of Ivermectin against gastrointestinal nematodes was shown 96.6% in Haemonchus contortus, 94.1% in Trichostrongylus spp. and 100% in Oesophagostomum spp., Ostertagia spp., Bunostomum trigonocephalum, Strongyloides papillosus and Cooperia spp.

• 보존과학회지
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• 제1권1호
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• pp.12-26
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• 1992

Ilsan peat-bed site, newly constructed city area, is located at the northern part of Seoul. This site is situated near the mouth of Han river, which flows through central Korea. Many fossil woods were excavated from this site, and surveyed the wood anatomical features. Totally 90 specimens were identified into 10 species including two roots and five barks which were not identified in detail. Alnus japonica and A. maxmowiczii are the most common and occupied 26. 7%, 34.4% of the total specimen number respectively, and then other species which excavated from Ilsan area are follows : Fraxinus rhynchophylla(18.9%), Lepido-balanus spp.(3.3%), Maackia amurensis(2.2%), Diospyros spp.(2.2%), Juglans mandshrica(1.1%), Prunophora spp.(1.1%), Malus or Pyrus spp.(1.1%), Rhamnus spp.(1.1%). Among the identified species there are fairly some species growing in the central - or northern temperate zone, such as A. maxmowiczii, F. rhynchophylla, Lepidobalanus spp., M. amurnsis, J. mandshurica, Rhumnus spp. Therefore, it can be estimated that the former temperature of Ilsan area was same or slightly cold than today. The species bearing fruit for food as .J, mandshunca, Diospyros spp., Prunophora spp., Mulus or Pyrus spp. were regarded as artificially planting trees, because they do not grow together in the same site.

• 한국동물위생학회지
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• 제15권1호
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• pp.81-88
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• 1992

This study was conducted to determine the microorganisms inhabitating in sow genital organs and their anti-microbial drug susceptibility During the period between February, 1991 and November 1991, 128 sow genital organs were sampled at six abattoirs. Gross pathological examination and bacterial isolation and identification were performed from the genital organ. In addition, antimicrobial drug susceptibility for the major organisms isolated were examined. 1. Among the bateria isolated from normal genital organs, E. coli(30.7%) Stahylococcus spp.(29.4%), Corynebarterium pyogenes(C. pyogenes) (14.7%), Streptococcus spp.(13.3%) were most freqently isolated, whereas the genera of Klebsiella, Actinobacillus, and Serratia were detected less freqently. 2. Among the bacteria isolated from abnormal genital organs, C. pyogenes,(37.7%), Stahylococcus spp.(30.2%), Proteus spp. (26.4%) , Pasteurella spp. (18.9%) , Steptococcus spp. (9.4%) were most freqently isolated whereas the genera of Pseudomonas, Serratia and Klebsiella were detected less freqently. 3. From sow genital organs showing lesion of endometritis and purulent endometritis C. pyogenes were most freqently isolated, the isolation rate being 67.7％ and followed by Stahylococcus spp., E. coli, Proteus spp., Steptococcus spp. and Pasteurella spp. in the order. 4. Antimicrobial drug susceptibility of the major organisms showed that all the isolates were susceptible to cephalothin, ampicillin, chloramphenicol and sulfamethoxazole / trimethoprim, but resistant to penicillin and streptomycin.

• 대한의생명과학회지
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• 제16권1호
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• pp.10-18
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• 2010

The present study was set to develop comprehensive system for assessing the safety of drinking water using PCR-reverse blot hybridization assay (REBA). The REBA developed in this study can detect waterborne pathogens such as Shigella spp., Salmonella spp., Citrobacter spp., Enterobacter spp., Klebsiella spp., Yersinia spp., Mycobacterium spp., Listeria spp. at the genus level, and Escherichia coli, Citrobacter freundii, Klebsiella pneumoniae, Pseudomonas aeruginosa, Yersinia enterocolitica, Y. pseudotuberculosis, Mycobacterium avium complex, M. marinum, Enterococcus faecalis, and Staphylococcus aureus at the species level, and E. coli O157:H7 at the strain level.

• Journal of Microbiology and Biotechnology
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• 제10권4호
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• pp.532-534
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• 2000

The inhibitory effects of different Bifidobacterium spp. on the growth of Helicobacter pylori (HP) were investigated. A significant suppression of HP growth occurred only when HP was inoculated onto a petri dish containing 0.1 mg/ml of Bifidobacterium spp. When HP was separately cultured with B. breve K-110, B. catenulatum K-309, B magnum K-311, B. magnum K-321, and B. cuniculi K-513, the urease activity was also inhibited by these Bifidobacterium spp. Therefore, it appears that these Bifidobacterium spp. excrete a heat-labile inhibitory component for HP growth into the culture medium. Although most organic acids produced by the Bifidobacterium spp. inhibited the growth of HP, the HP growth was not inhibited by the physiological concentrations of organic acids produced in bifidobacteria-cultured media. Accordingly, these results suggest that some Bifidobacterium spp. may produce antibiotic-like compounds (bacteriocins).

• 한국미생물·생명공학회지
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• 제25권5호
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• pp.435-441
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• 1997

Forty seven strains of yeast were isolated from traditional Meju and were identified as Saccharomyces spp. (7 strains), Zygosaccharomyces spp. (7 strains), Kluyveromyces spp. and Hansenula spp. (each 5 strains), Rhodotorular spp. (8 strains), Candida spp. (12 strains), Pichia spp. and Debaryomyces spp. from results of their microbiological characteristics. The optimal medium for growth of all the yeasts was YM media and the optimal initial pH of the medium was 6.0. The optimum temperature for growth was 30$circ$ and among them, Sacch. exiguus OE-5, Sacch. cerevisiae OE-16, Sacch. kluyveri C-1 strains were thermotolerant yeasts which could grow at 40$circ$C.

• 대한수의학회지
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• 제18권1호
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• pp.27-31
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• 1978

In order to identify unknown Babesia spp. which was isolated from Korean cattle, the morphology of Korean strain was compared with that of Babesia bigemina (Kochinda strain) and Babesia spp. (Miyake strain). Immunofluorescent technique was used to identify the serological character of the parasites. The results obtained were summarized as follows: 1. Korean strain was morphologically very similar to Babesia spp.(Miyake strain) which mostly showed parallel-bigeminate forms, while B. bigemina (Kochinda strain) was mostly round and oval forms. 2. By the indirect fluorescent antibody technique: a) Anti-Babesia spp. and Korean Babesia spp. sera showed a higher antibody titers with Babesia spp. (Miyake strain) antigen (1:500) than with B. bigemina (Kochinda strain) antigen (1:50). b) Anti-Babesia bigemina sera showed a lower titer with Babesia spp. antigen (1:50) than with B. bigemina antigen (1:250). 3. On the basis of morphological and serological confirmantions, a Babesia strain isolated from a Korean cattle was very similar, if not identical, to Miyake strain of Babesia spp.

• 대한수의학회지
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• 제38권1호
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• pp.217-225
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• 1998

영국 Hertfordshire의 두지역에서 사육되고 있는 건강한 조랑말의 결막낭내 상존하고 있는 진균총의 분포도를 조사 비교하고자 36두 조랑말의 양안(총72안)의 상 하 결막낭에서 시료를 채취하여 진균을 분리 동정하였다. 총 76.4%의 안구에서 진균이 발견되었으며, 13종이 분리되었다. 전체적으로 가장 많이 분리된 진균은 Aspergillus spp(27.8%), Nocardia spp(25.0%), Cladosporium spp(19.4%), Penicillium spp(9.7%) 및 Penicillium spp(9.7%)순이었다. 1지역에서는 Nocardia spp와 Cladosporium spp가 가장 많이 분리된 반면, 2지역에서는 Aspergillus spp가 가장 많이 분리되었다. 상 하결막낭내 진균분리율의 차이는 상결막낭에서보다는 하결막낭내에서 진균이 유의성 있게 많이 분리되었다(p<0.05). 시료채취시에 사용된 swab의 상태에 따른 비교에서 건조된 swab과 젖은 swab간에는 통계적 유의성이 없었다.

• 한국식품영양학회지
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• 제28권4호
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• pp.728-736
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• 2015

Cronobacter spp.는 Salmonella spp.와 함께 조제분유에서 발견되는 미생물 중 위험도가 가장 높은 category A에 속하는 균으로 알려져 있다. 1958년 영국에서 처음으로 유아의 뇌수 막염의 원인균으로 보고되었으며, 감염 후 신생아에게서 괴사성 장염, 패혈증 등을 일으켜 후유증으로 시력과 청력 상실 및 신경마비를 일으키기도 한다. 이러한 위험성 때문에 Cronobacter spp.의 신속 검출 및 진단은 식중독 예방에 있어서 중요하다. 따라서 2002년 미국 식품의약품안전국에서는 분유에서의 Cronobacter spp.를 EE broth, VRBG 배지를 이용해 균을 분리 하고, TSA 배지에 순수분리 후 노란색을 발하는 colony를 생화학적 실험을 통해 검출하는 방법을 제시했다. 또한, 우리나라 식품공전에서도 배지를 이용하여 Cronobacter spp.를 검출 하는 방법에 대해 등재하였다. 하지만 배지배양법을 기반으로 하는 Cronobacter spp.의 검출방법은 검출에 소요되는 시간과 노동력 등이 비효율적이라는 단점이 있다. 따라서 많은 시간이 소요되는 배지배양법을 보완하고자 PCR, real-time PCR 방법 및 최근에는 PCR기법과 ELISA, CE-LIF 등을 결합하여 검출하는 방법이 개발되어 Cronobacter spp.의 검출에 사용되기도 하였다. Cronobacter spp.의 검출에 사용되고 있는 분자생물학적 기반의 PCR 및 real-time PCR 기법은 민감도와 특이도가 좋으며, 배지배양법에 의한 검출방법에 비해 검출 시간을 획기적으로 줄일 수 있다는 장점이 있다. 하지만 까다로운 실험과정과 조작에 있어서 필요한 전문적인 기술이 필요함은 한계점으로 사료된다. 면역학적 방법에 의한 Cronobacter spp. 검출에 관한 연구를 통해, 분석에 소요되는 상당시간을 단축할 수 있었으며, 민감하고 특이적인 검출이 가능함을 보여주었다. 특히 일부 연구보고에서 면역학적 검출방법은 고가의 장비가 필요 없으며, 간단한 지침에 따라 모니터링 업무의 수행이 가능하다고 하였다. 면역학적 검출방법에는 ELISA 방법 외에도 immunomagnetic bead, liposome, immunochromatographic strip 등이 개발되고 있다. 우리나라 식품의약품안전처에서는 영 유아 대상 식품의 안전관리를 강화하고자 Cronobacter spp.에 대해서는 '불검출'로 기준을 설정 운영하고 있으며, 지속적으로 이에 관한 규정을 강화하고 있는 실정이다. 따라서, 식품산업체 및 식품 의 제조, 가공, 유통 현장에서 쉽게 모니터링이 가능하며, 신속, 민감하고 특이적인 검출방법의 개발은 지속되어야 한다.

• Journal of Dairy Science and Biotechnology
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• 제35권3호
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• pp.152-161
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• 2017

Cheese is an excellent substrate for yeast and mold growth. These organisms can cause cheese spoilage, resulting in significant food wastage and economic losses. In the context of cheese spoilage, the presence and effects of spoilage or pathogenic bacteria are well documented. In contrast, although yeasts and molds are responsible for much dairy food wastage, only a few studies have examined the diversity of spoilage fungi. This article reviews the spoilage yeasts and molds affecting cheeses in various countries. The diversity and number of fungi present were found to depend on the type of cheese. Important fungi growing on cheese include Candida spp., Galactomyces spp., Debaryomyces spp., Yarrowia spp., Penicillium spp., Aspergillus spp., Cladosporium spp., Geotrichum spp., Mucor spp., and Trichoderma spp.. In addition, several mold spoilage species, such as Aspergillus spp. and Penicillium spp., are able to produce mycotoxins, which may also be toxic to humans. There are many ways to eliminate or reduce toxin levels in foods and feeds. However, the best way to avoid mycotoxins in cheese is to prevent mold contamination since there are limitations to mold degradation or detoxifications in cheese. Chemical preservatives, natural products, and modified atmosphere packaging have been used to prevent or delay mold spoilage and improve product shelf life and food safety.