• 대한수의학회지
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• 제53권1호
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• pp.25-28
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• 2013

The aim of this study was to applicate and evaluate a SYBR Green real-time PCR for the specific detection of Salmonella spp. Specificity of the PCR method was confirmed with 48 Salmonella spp. and 5 non-Salmonella strains using invA gene primer. The average threshold cycle ($C_T$) of Salmonella spp. was $11.83{\pm}0.78$ while non-Salmonella spp. was $30.86{\pm}1.19$. Correlation coefficients of standard curves constructed using $C_T$ versus copy number of Salmonella Enteritidis ATCC 13076 showed good linearity ($R^2=0.993$; slope = 3.563). Minimum level of detection with the method was > $10^2$ colony forming units (CFU)/mL. These results suggested that the SYBR Green real-time PCR might be applicable for the specific detection of Salmonella spp. isolates.

• 한국산업보건학회지
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• 제16권2호
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• pp.101-109
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• 2006

The objective of this study is to provide fundamental data for pertinent management of indoor air quality through investigating the size-based characteristics of bioaerosol distributed in the general hospital. Measurement sites are main lobby, ICU, ward and laboratory and total five times were sampled with six-stage cascade impactor. Based on the result of this study, concentrations of airborne bacteria and fungi were the highest in main lobby as followed by an order of ward, ICU and laboratory. Concentrations of airborne bacteria was generally higher than those of airborne fungi and the ratio of indoor and outdoor concentration of both exceeded 1.0 in all the measurement sites of the general hospital. The predominant genera of airborne bacteria identified in the general hospital were Staphylococcus spp.(50%), Micrococcus spp.(15-20%), Corynebacterium spp.(5-20%), and Bacillus spp.(5-15%). On the other hand, the predominant genera of airborne fungi identified in the general hospital were Cladosporium spp.(30%), Penicillium spp.(20-25%), Aspergillus spp.(15-20%), and Alternaria spp.(10-20%). In regard to size distribution of bioaerosol, the detection rate was generally highest on 5 stage($1.1-2.1{\mu}m$) for airborne bacteria and on 1 stage(>$7.0{\mu}m$) for airborne fungi. Cleanliness of facilities in the general hospital and condition of HVAC system should be monitored regularly to prevent indoor air contamination by airborne microorganisms.

• 한국식품위생안전성학회지
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• 제17권4호
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• pp.173-177
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• 2002

2002년 3-5월 서울시 가락농수산물시장에서 구입한 젓갈류 72건(새우젓류 21건, 명태젓류 9건 및 조개젓류 9건, 오징어젓, 밴댕이젓 및 멸치젓 각각 7건, 황세기젓 5건, 꼴뚜기젓 3건 그리고 굴젓 및 갈치젓 각각 2건)을 대상으로 세균분포를 시험한 결과, 평균 염도는 17.2%, 1 ml당 일반세균수는 4,900, 대장균군수 44, 비브리오속균수 160, 포도상구균속균수 3,000 CFU/m1로 각각 집계되었고, 젓갈별 세균 분리 동정결과, 오징어젓에서 13주, 꼴뚜기젓과 추젓에서 각각 11주, 명란젓과 조개젓에서 각각 9주, 멸치젓에서 7주 등 총 93주의 세균이 분리되었다. 세균의 생화학적 분리 동정 결과 대장균군 35.5%(33/93), 비브리오속균 8.6%(8/93) 그리고 포도상구균속균 12.9%(12/93)로 분리되었고, 대장균군의 경우 E. cloacae가 15주로 가장 많았으며, 비브리오속균의 경우 V. alginolyticus가 5주, V. fluvialis가 3주, 포도상구균속균의 경우 S. lentus가 5주로 가장 많았다.

• Mycobiology
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• 제44권2호
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• pp.99-104
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• 2016

Candida spp. is an invasive infectious fungus, a major risk factor that can increase morbidity and mortality in hospitalized patients. In this study, 2,508 Candida spp. were isolated from various clinical specimens collected from university hospitals from July 2011 to October 2014. They were identified in order to determine isolation frequencies and characteristics by specimen, gender, age group, year, season, and month. The strain-specific isolation rate of Candida spp. is in the order of Candida albicans (1,218 strains, 48.56%), Candida glabrata (416 strains, 16.59%), Candida utilis (305 strains, 12.16%), Candida tropicalis (304 strains, 12.12%), and Candida parapsilosis (116 strains, 4.63%) and these five species accounted for more than 94% of the total strains. Of the specimens, Candida spp. were most frequently isolated from urine-catheter, followed by urinevoided, blood, sputum, other, open pus, vaginal discharge, Tip, ear discharge, bronchial aspiration and bile, in that order. Looking at the age distribution, the detection rate of patients in their 60s and older was significantly higher at 75.8% (1,900/2,508). The detection rate of patients in their 20s and younger was shown to be very low at 2.55% (64/2,508). By year, the detection rate of non-albicans Candida spp. showed a tendency to gradually increase each year compared with C. albicans. As isolation of Candida spp. from clinical samples at the specie level can vary depending on characteristics of the patient, sample, season, etc., continual studies are required.

• 농업과학연구
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• 제40권2호
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• pp.101-106
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• 2013

To know how much damages occurred by nematodes in plastic vinyl house field, soil samples were collected from strawberry cultivation areas at Buyeo and Nonsan in Chungnam and Jinju in Kyeongnam, melon cultivation area at Gocksung in Junnam and cucumber field at Gongju in Chungnam. And then, nematode samples were separated from each soil sample, and identified the kind of plant-parasitic nematodes. Plant-parasitic nematodes were separated from 52 soil samples. Among samples, Meloidogyne spp., Pratylenchus spp. and Helicotylenchus spp. were isolated from 45 (86.5%%) and 33 (63.5%) and 47 collected soil samples (90.4%), respectively. As a result of identification of plant-parasitic nematodes from regional collected soil samples, distribution of Helicotylenchus spp. was higher than any other plant-parasitic nematode. And the population of Meloidogyne spp. and Pratylenchu spp. were also higher, and a occurrence ratio of Meloidogyne spp. is higher than Pratylenchu spp. except the cucumber growing area at Gongju.

• 한국동물위생학회지
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• 제27권2호
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• pp.159-164
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• 2004

A dairy farm that has been suffered continuously(more than 2 years) from brucellosis in Korea in spite of repeated legal test-and-slaughter was investigated the main source of infection in the farm. All cattle(22 milking cows, 44 heifers, 60 calves, 8 bull), dogs(3 mixed breed), feces from wild birds(3 samples), drinking water(3 sites), and soil in the paddocks(14 sites) inside the farm were examined with serological and/or bacteriological methods including specific DNA detection with PCR method. Brucella spp in the milk and blood were detected in 12/22 and 5/22 milking cows, respectively, although all of them were negative with conventional tube agglutination test. The number of serologically positive heifer was 15(15/44), but the isolation of Brucella spp was succeeded in the only 11(11/15) of them. Brucella were detected in vagina 1(1/11) and nasal(3/12) excretion in serologically positive heifers. All the three dogs were serologically positive, and Brucella spp were isolated from their blood. However, Brucella spp were not detected in the drinking water, soil in the paddocks, nor the feces of wild birds. The results suggest that milking cow secrete Brucella spp through milk, genital tract and nasal cavity, which are the major source of infection in this farm, The main infection route of Brucella spp is contact to contact with Brucella spp excreting animals rather than environmental contamination. The animals, living together with infected cow such as dogs, are the readily susceptible and are required to be examined for Brucella spp.

• 한국응용곤충학회지
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• 제21권2호
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• pp.68-72
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• 1982

인삼 재배포장에서 병원균인 Fusarium spp.의 밀도와 근부에 영향을 미치는 토양환경요인을 조사하였다. 2년근 포장의 토양을 토성별로 분류하여 결주율, Fusarium spp. 밀도, 화학적 성질을 비교해 본 결과 점토 함량이 들었고 결주율과 질산태 질소는 줄어드는 경향이었으며 다른 성질은 뚜렷한 차이가 없었다. 6년근 포장에서는 근부율과 유효인산 함량이 정의 상관(r=0.3162, p=0.05)을 보였으며 Fusarium spp. 밀도와 Streptomyces spp. 밀도는 고도의 부의 상관(r=-0.3976, p=0.01)을 나타내었다.

• 한국수산과학회지
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• 제38권1호
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• pp.55-64
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• 2005

Spatio-temporal variations of phytoplankton abundance and nutrient concentrations were investigated in the Namdaechon estuary, Yangyang, from April to December, 2003. A total of 51 phytoplankton species were identified with 32 diatom species in the study area. Phytoplankton abundance ranged from 14 cells/mL to 3,798 cells/mL. Small sized benthic and pennate-type diatoms like Cymbella spp., Fragilaria spp., Navicula spp., Synedra spp. were dominant at throughout the whole study area. Various planktonic species like Chlamydomonas sp., Peridinium spp., Euglena spp., Cryptomonas spp. etc. were abundant especially at the estuary of the Namdaechon, from May to August. Phytoplankton bloom (>3,000 \;cells/mL) occurred from the late May to mid June. After September when the sandbank of estuary was broken by the typhoon 'Maemi', planktonic species disappeared. These planktonic species were followed by oceanic diatoms, Skeletonema costatum and Chaetoceros spp.. Concentrations of dissolved inorganic nitrogen (DIN) and silicate were relatively high at the upstream in summer. In May, before phytoplankton bloom, high concentrations of phosphate $(>3.5\;{\mu}mol/L)$ were observed at all the study area. These results suggested that spatio-temporal variation of phytoplankton in the Namdaechon estuary was related to formation of sandbank at the mouth of the estuary, fluid speed affected by sandbank and nutrients supplied in spring. High correlation $(r^2=0.928)$ between chlorophyll a and biological oxygen demand (BOD) implies that BOD was related to phytoplankton abundance in the Namdaechon estuary.

• Parasites, Hosts and Diseases
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• 제57권5호
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• pp.553-559
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• 2019

The Alataw Pass, near the Ebinur Lake Wetland (northwest of China) and Taldykorgan (east of Kazakhstan), is a natural habitat for wild rodents. To date, little has been done on the surveillance of Bartonella spp. and Wolbachia spp. from fleas in the region. Here we molecularly detected Bartonella spp. and Wolbachia spp. in wild rodent fleas during January and October of 2016 along the Alataw Pass-Kazakhstan border. A total of 1,706 fleas belonging to 10 species were collected from 6 rodent species. Among the 10 flea species, 4 were found to be positive for Wolbachia, and 5 flea species were positive for Bartonella. Molecular analysis indicated that i) B. rochalimae was firstly identified in Xenopsylla gerbilli minax and X. conforms conforms, ii) B. grahamii was firstly identified in X. gerbilli minax, and iii) B. elizabethae was firstly detected in Coptopsylla lamellifer ardua, Paradoxopsyllus repandus, and Nosopsyllus laeviceps laeviceps. Additionally, 3 Wolbachia endosymbionts were firstly found in X. gerbilli minax, X. conforms conforms, P. repandus, and N. laeviceps laeviceps. BLASTn analysis indicated 3 Bartonella species showed genotypic variation. Phylogenetic analysis revealed 3 Wolbachia endosymbionts were clustered into the non-Siphonaptera Wolbachia group. These findings extend our knowledge of the geographical distribution and carriers of B. rochalimae, B. grahamii, B. elizabethae, and Wolbachia spp. In the future, there is a need for China-Kazakhstan cooperation to strengthen the surveillance of flea-borne pathogens in wildlife.

• Parasites, Hosts and Diseases
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• 제57권3호
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• pp.273-281
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• 2019

The infection status of digenetic trematode metacercariae (DTM) was investigated in fishes from 2 representative visiting sites of migratory birds in Gyeongsangnam-do, the Republic of Korea (Korea). A totaly 220 freshwater fishes (7 species) were collected from Junam-jeosuji (reservoir), and 127 fishes (7 species) were also collected from Woopo-neup (swamp) in June and October 2017. As the control group, total 312 fish (22 spp.) from Yangcheon in Sancheong-gun, Gyeongsangnam-do were also collected in June and October 2017. All fishes collected in 3 sites were examined with the artificial digestion method. In the fishes from Junam-jeosuji, more than 4 species, i.e., Clonorchis sinensis, Echinostoma spp., Diplostomum spp. and Cyathocotyle orientalis, of DTM were detected and their endemicy was very low, 0.70. More than 6 species, i.e., C. sinensis, Echinostoma spp., Metorchis orientalis, Clinostomum complanatum, Diplostomum spp. and C. orientalis, of DTM were found in the fishes from Woopo-neup, and their endemicy was low, 5.16. In the fishes from Yangcheon, more than 8 species, i.e., C. sinensis, Metagonimus spp., Centrocestus armatus, C. complanatum, C. orientalis, M. orientalis, Echinostoma spp., and Diplostomum spp., of DTM were detected, and their endemicity was relatively high, 95.48. The percentages of avian trematode metacercariae (ATM) were 99.6% and 94.7% in fishes from Junam-jeosuji and Woopo-neup whereas it was 74.1% in the control site, Yangcheon. The above findings suggested that migratory birds partly affect in endemicity of DTM in the fish in the 2 visiting sites in Gyeongsangnam-do, Korea.