• 한국환경농학회지
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• 제32권1호
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• pp.48-54
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• 2013

수수 종자의 품종 간 특이적으로 발현하는 단백질을 동정하여 기능성 유전자를 확보하고 이들 유전자를 이용하여 수수의 기능성 강화 및 품종 판별 기술 개발을 위한 유용 유전자를 확보하고자 프로테오믹스 기법을 이용하여 수수 종자로부터 단백질을 추출하였다. 추출한 단백질을 이차원전기영동후, colloidal CBB 염색을 통해 품종 별로 발현에 차이를 보이는 단백질을 분석하였다. 총 652 개의 spot들 중에 8개의 단백질 spot들이 발현 정도에 변화를 보였으며, 이들 단백질을 MALDI-TOF/TOF MS와 MASCOT database를 통해 동정한 결과, RNA metabolism (spot 1, spot 4) HSP (spot 2), 저장 단백질 (spot 3, spot 5, spot 6), 산화-환원 (spot 8) 관련 단백질 등이 동정되었다. 특히 동정된 단백질은 주로 흰찰수수 (WCS)에서 발현 정도가 높게 나오는 경향을 보였으며, 흰찰수수 (WCS)에서 유일하게 발현 되는 단백질로 Cupin family protein, Gloubulin 등이 동정되었다. DEAD-box helicase는 흰찰수수 (WCS)를 제외한 나머지 세 품종에서 발현되었다. Ribonuclease T2와 Aldo-Keto reductase는 대풍수수 (DPS)를 제외한 나머지 세 품종에서 발현되었다. HSPs는 토종수수 (TJS)에서만 발현 되는 것을 확인하였다. 이들 동정된 단백질들은 수수의 품종 별 특성을 이해하는데 중요한 단서를 제공할 것으로 예측된다.

• The Journal of Advanced Prosthodontics
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• 제5권3호
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• pp.359-363
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• 2013

This article describes the treatment provided to a patient with the maxillary anterior teeth exhibiting severe secondary caries beneath the previous restoration and a white spot lesion on the adjacent incisor. Two implants were placed after extraction of hopeless teeth with the guided bone regeneration technique. A white spot lesion of the adjacent incisor was treated with minimally invasive treatment. This clinical report describes the multidisciplinary treatment for the white spot lesion and esthetic restoration of missing anterior teeth.

• Current Optics and Photonics
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• 제3권5호
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• pp.458-465
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• 2019

The thermal effect and the light output of a laser crystal under different pumping depths were reported., Based on the thermal model of a single-ended pumped Tm:YAP crystal, the thermal stress coupled model used Comsol to theoretically calculate the effect of changing the pump spot size and pump depth on crystal heat distribution and stress distribution. The experimental results showed that the laser output power first increased and then decreased with increasing pump spot size. As the depth of focus increased, the laser output power first increased and then decreased. The experimental results were consistent with the theoretical simulation results. The theory of pump spot radius and depth of focus in this paper provided an effective simulation method for mitigating thermal effects, and provided theoretical supports for laser crystals to obtain higher laser output power.

• Journal of Welding and Joining
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• 제19권5호
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• pp.499-505
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• 2001

In order to classify the mechanical phenomena of thermal elasto-plastic behavior on the multi-spot welded joints, this study has tried to carry out three-dimensional thermal elasto-plastic analysis on them. However, because the shape of multi-spot welded joints is not taxi-symmetric, unlike the cafe of single-spot welded joint, the solution domain for simulation should be three dimension. Therefore, in this paper, firstly, the three-dimensional thermal elasto-plastic program is developed by an iso-parametric finite element method. Secondly, from the results analyzed by developed program, this has clarified mechanical characteristics and their production mechanism on single and multi-spot waled joints. Moreover, it has been intended to make clear effects of pitch length on welding residual stresses, plastic strain of multi-spot welded joints.

• 한국약용작물학회지
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• 제22권5호
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• pp.398-404
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• 2014

Salt stress is one of the major abiotic stresses affecting the yield of ginseng (Panax ginseng C. A. Meyer). The objective of this study was to identify bio-marker, which is early responsive in salt stress in ginseng, using proteomics approach. Ginseng plants were exposed to 5 ds/m salt concentration and samples were harvested at 0, 6, 12 and 18 hours after exposure. Total proteins were extracted from ginseng leaves treated with salt stress using Mg/NP-40 buffer and were separated on high resolution 2-DE. Approximately $1003{\pm}240$ (0 h), $992{\pm}166$ (6 h), $1051{\pm}51$ (12 h) and $990{\pm}160$ (18 h) spots were detected in colloidal CBB stained 2D maps. Among these, 8 spots were differentially expressed and were identified by using MALDI-TOF/TOF MS or/and LC-MS/MS. Ethylene response sensor-1 (spot GL 1), nucleotide binding protein (spot GL 2), carbonic anhydrase-1 (spot GL 3), thylakoid lumenal 17.9 kDa protein (spot GL 4) and Chlorophyll a/b binding protein (spot GL 5, GL 6) were up-regulated at the 12 and 18 hour, while RuBisCO activase B (spot GL 7) and DNA helicase (spot GL 8) were down-regulated. Thus, we suggest that these proteins might participate in the early response to salt stress in ginseng leaves.

• 한국지리정보학회지
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• 제2권1호
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• pp.32-44
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• 1999

본 연구의 목적은 질감을 분광정보와 함께 사용했을 때의 분류정확도의 향상을 평가하는데 있다. 먼저 부산지역의 SPOT HRV 영상에 최대우도분류를 적용하여 토지피복도를 작성하였다. 그리고 3번 파장에서 다양한 질감을 추출한 다음, 이 질감을 신파장의 형태로 분광정보에 통합하여 분류하여 질감의 사용이 분류의 정확도에 미치는 영향을 질감별로 평가하였다. 정확도 평가는 전체적인 정확도와 토지피복별 정확도로 구분하였다. 연구결과 전체적인 정확도 향상을 관측할 수 있었는데, 특히 엔트로피의 개선 효과가 우수하였다. 그리고 창의 크기는 $5{\times}5$와 $7{\times}7$이 적절한 것으로 나타났다. 그리고 질감에 따라서는 전체적인 정확도는 향상되지 않더라도 일부 토지피복의 정확도는 개선되는 것으로 나타났다. 토지피복별로는 저층건물지역, 아파트 단지. 고층건물지역, 공업지역 등 도시지역의 개선효과가 높은 것으로 나타났다.

• 한국측량학회지
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• 제28권2호
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• pp.255-263
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• 2010

북한의 농업용 저수지 개발방향을 설정하고 농촌종합개발사업 및 농지현황분석 등 농업대책정보 수요에 보다 신속하고, 효과적으로 대처할 수 있도록 기술적, 정책적 방향을 수립하는 것은 남북교류 및 통일을 대비하여 중요한 의미가 있다. 본 연구의 목적은 SPOT-5 위성영상을 처리하여 북한 예성강 유역내 농업생산기반 DB를 구축하고 농업용 저수지 현황을 분석하는데 있다. 연구 결과, 현장방문조사가 불가능한 예성강유역의 농업용수관련 현황을 분석하여 분류항목을 설계하고 아울러 실제 농업생산기반관련 주제도를 제작하는 방법 및 절차를 정립할 수 있었다. 그리고, 유역추출, 기설 저수지 용적계산, 전답의 규모 및 수혜지역(생산지역) 범위 설정, 기존의 농업용수체계 등을 조사하여 농업용 저수지의 개발적지 분석을 위한 기초자료를 제공하였다. 또한, 북한지역의 농업생산기반 DB 구축시 문제점과 한계를 파악할 수 있었다.

• 한국응용곤충학회지
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• 제39권4호
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• pp.259-266
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• 2000

This study was carried out to investigate the status of spot damage by fruit piercing pests and the kinds of these pests on yuzu (Citrus junos) fruit in Koheung, the most chief producing district of yuzu fruit in Korea, from ’97 to ’99. The extent of spot damage by fruit piercing pests on yuzu was increasing in recent years. This damage of fruits was severe in the lower canopy than the high one from ground and intercropping groves between yuzu trees had a greater damage to compare with single cropping of yuzu. Spot damage of yuzu fruit was occurred mainly from late September to early November when yuzu fruit is enlarging and coloring yellow. The blackish concave spot on yuzu fruit surface was appeared in 3 days after introduction of Riptortus clavatus into a netted cage containing one yuzu fruit and the diameters of this spot was 8.3 mm. At 10 days after introduction, this spot changed into milky-white with 9.2 mm diameters. One concave spot has contained oil cells by 17.7 and its external appearances has unchanged until harvest. The kinds of piercing pests of yuzu fruit were investigated with 3 orders, 16 families and 37 species. These pests were classified by 11 species of bugs, 5 species of hoppers and 21 species of moths. Among them, dominant species were Physopelta gutta, Halyamorpha halys, Empoasca vitis, Aedia leucomelas, Agrotis tokionis, etc. Macroglossum bombylans and Acherontia s쇼x are newly confirmed species as the fruit piercing moths in Korea.

• International Journal of Automotive Technology
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• 제5권1호
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• pp.55-59
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• 2004

Spot welding is a very important and useful technology in fabrication of thin sheet structures such as the parts in an automobile. However, because the fatigue strength of the spot welding point is considerably lower than that of the base metal due to stress concentration at the nugget edge, the nugget size must be estimated to evaluate a reasonable fatigue strength at a spot welded lap joint. So far, many investigators have experimentally studied the estimation of fatigue strengths of various spot weldments by using a destructive method. However, these destructive methods poses problems so testing of weldments by these methods are difficult. Furthermore, these methods cannot be applied to a real product, and are time and cost consuming, as well. Therefore, there has been a strong, continual demand for the development of a nondestructive method for estimating nugget size. In this study, the effective nugget size in spot weldments have been analyzed by using thermoelastic stress analysis adopting infrared thermography. Using the results of the temperature distribution obtained by analysis of the infared stress due to adiabatic heat expansion under sinusoidal wave stresses, the effective nugget size in spot welded specimens were estimated. To examine the evaluated effective nugget size in spot weldments, it was compared with the results of microstructure observation from a 5％ Nital etching test.

• 약학회지
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• 제50권4호
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• pp.268-271
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• 2006

Protein kinase UL97 is an unusual protein kinase that can phosphorylate nucleoside analogs as well as protein/peptide. Previously we found a H2B-derived peptide, KESYSVYVYKV and reported that the P+5 position (K) is important. To further understand the substrate specificity at the P+5 position, we introduced spot assay system and showed that a peptide containing K residue among other amino acids at the P+5 position is the best substrate. Also other residues such as M, I, L, or G are good enough to be substrate of UL97. This result may aid the discovery of a new antiviral inhibitor.