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http://dx.doi.org/10.7783/KJMCS.2014.22.5.398

Proteomics Analysis of Early Salt-Responsive Proteins in Ginseng (Panax ginseng C. A. Meyer) Leaves  

Kim, So Wun (Department of Plant Bioscience, Pusan National University)
Min, Chul Woo (Department of Plant Bioscience, Pusan National University)
Gupta, Ravi (Department of Plant Bioscience, Pusan National University)
Jo, Ick Hyun (Ginseng Research Division, Department of Herbal Crop Research, NIHHS, RDA)
Bang, Kyong Hwan (Ginseng Research Division, Department of Herbal Crop Research, NIHHS, RDA)
Kim, Young-Chang (Ginseng Research Division, Department of Herbal Crop Research, NIHHS, RDA)
Kim, Kee-Hong (Ginseng Research Division, Department of Herbal Crop Research, NIHHS, RDA)
Kim, Sun Tae (Department of Plant Bioscience, Pusan National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.22, no.5, 2014 , pp. 398-404 More about this Journal
Abstract
Salt stress is one of the major abiotic stresses affecting the yield of ginseng (Panax ginseng C. A. Meyer). The objective of this study was to identify bio-marker, which is early responsive in salt stress in ginseng, using proteomics approach. Ginseng plants were exposed to 5 ds/m salt concentration and samples were harvested at 0, 6, 12 and 18 hours after exposure. Total proteins were extracted from ginseng leaves treated with salt stress using Mg/NP-40 buffer and were separated on high resolution 2-DE. Approximately $1003{\pm}240$ (0 h), $992{\pm}166$ (6 h), $1051{\pm}51$ (12 h) and $990{\pm}160$ (18 h) spots were detected in colloidal CBB stained 2D maps. Among these, 8 spots were differentially expressed and were identified by using MALDI-TOF/TOF MS or/and LC-MS/MS. Ethylene response sensor-1 (spot GL 1), nucleotide binding protein (spot GL 2), carbonic anhydrase-1 (spot GL 3), thylakoid lumenal 17.9 kDa protein (spot GL 4) and Chlorophyll a/b binding protein (spot GL 5, GL 6) were up-regulated at the 12 and 18 hour, while RuBisCO activase B (spot GL 7) and DNA helicase (spot GL 8) were down-regulated. Thus, we suggest that these proteins might participate in the early response to salt stress in ginseng leaves.
Keywords
Ginseng; Protein Biomarker; Proteomics; Salt Stress; 2-DE;
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Times Cited By KSCI : 5  (Citation Analysis)
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