• 한국자원식물학회지
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• 제9권3호
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• pp.203-210
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• 1996

더덕 뿌리 내에 함유되어 있는 항산화효소를 생육환경에 따라 조사하기 위하여 경북 서벽지역의 1 2년생 밭더덕, 야생더덕 및 논에서 재배되는 1 2년생 더덕을 채취하여 사용하였고, 부위별 비교는 각 더덕 뿌리를 upper, middle, low로 나누어서 사용하였다. 이들을 O radical caseade인 SOD, POD, CAT activity를 조사하여 다음과 같은 결과를 얻었다. 1. SOD 활성은 밭재배조건의 경우 1년생보다는 2년생이, 논재배시는 1년생이 2년생보다는 높은 활성을 나타내었으며, 야생종의 비활성이 96069u/mg protein으로 가장 높은 활성을 나타내었다. 부위별로 비교해보면 재배종은 upper>middle>lower 순으로 논재배시는 low>upper>middle 순으로 야생종은 middle>lower, upper 순으로 생육환경에 따라 차이를 나타내었다. 2. POD 비활성(u/mg protein)은 년근수가 높을수록 활성은 증가하며, 야생지역이 68 u/mg protein로 가장 높은 활성을 나타내었다. 부위별로는 middle>lower>upper 순으로 활성이 나타났으며, 서벽야생의 middle 부분이 85 u/mg protein를 나타내었다. 3. CAT 활성은 POD 활성과는 달리 년근수가 낮을수록 높게 나타났으며 밭재배지와 야생지의 활성은 비슷하며 논재배지는 상대적으로 그 활성이 낮았다. 부위별로는 upper>low>middle 순으로 upper 부위가 상당히 높은 활성을 나타내며, 서벽재배 1년생의 upper가 53.59 u/mg protein으로 가장 높게 나타났다. 4. MS1D(MS+2.4-D 1ppm)에 배양한 배양세포의 항산화효소의 활성은 CAT는 1564, POD는 30, SOD는 22200 u/mg protein로 생체에 비해 낮은 활성을 나타내었다.

• 생명과학회지
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• 제31권6호
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• pp.537-542
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• 2021

자웅이주 식물로 알려진 Schisandra nigra Max. (흑오미자)는 우리나라 제주도에 자생하고 있으며, 열매를 얻기 위해 일부 농가에서 재배되고 있다. 열매 생산을 위해서는 수그루에 비해 암그루의 가치가 더 높기 때문에 묘목단계에서 일찍 성별을 아는 것은 중요하다. 이 연구에서는 S. nigra의 유전체에서 암그루에 특이적인 부위에 관련된 SCAR 마커를 개발하였다. 120개의 무작위로 구성된 RAPD 프라이머 중에서 OPB-03 프라이머가 암그루에서 749 bp의 밴드를 안정적으로 증폭시켰다. 암그루 특이적인 PCR 산물을 분리하여 클로닝한 뒤 염기서열을 결정하였다. 이 암그루 특이적인 절편을 탐침으로 사용한 Southern hybridization에서 암그루에서만 양성반응이 나타나고 수그루에서는 잡종화가 일어나지 않았다. 이러한 결과는 749 bp의 DNA 절편이 암그루의 유전체에는 존재하지만, 수그루에는 없음을 시사하였다. RAPD 마커로부터 암그루에서만 436 bp를 증폭시키는 SCAR 프라이머를 설계하였다. 이 프라이머 쌍은 암그루와 자생지에서 수집한 4개의 자웅동주 식물에서만 예상되었던 크기의 DNA 절편을 증폭하였다. 이 연구에서 개발된 SCAR 마커는 묘목 단계에서 암꽃이 피는 개체를 선발하는데 사용될 수 있을 것이다.

• 한국환경복원기술학회지
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• 제20권3호
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• pp.33-44
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• 2017

This study investigated in the vascular plants of special protection zones (the native land of Berchemia berchemiaefolia in Sadam-ri and Gallon valley) of Songnisan National Park and identified the whole vascular plants. The numbers of vascular plants were summarized as 273 taxa including 82 families, 182 genera, 236 species, 3 subspecies, 28 varieties and 6 forms. Woody plants were identified as 124 taxa (45.4%) and herbaceous plants as 149 taxa (54.6%). A total of 107 taxa were identified in the area of Sadam-ri, and 246 taxa were found in the Gallon valley. A total of 8 taxa of rare plants were identified, each of which was divided into 1 taxa of endangered (EN; Daphne pseudomezereum var. koreana), 3 taxa of vulnerable (VU; Paeonia japonica, Berchemia berchemiaefolia and Iris minutiaurea), 3 taxa of least concern (LC; Aristolochia contorta, Syringa patula var. kamibayshii and Goodyera schlechtendaliana) and 1 taxa of data deficient (DD; Alangium platanifolium). The Korean endemic plants were 9 taxa (Clematis trichotoma, Thalictrum actaefolium var. brevistylum, Aster koraiensis, Cirsium setidens, etc.). In the specific plants by floristic region were 34 taxa, a degree I were 17 taxa (Chloranthus japonicus, Celastrus stephanotifolius, etc.), 9 taxa of a degree II (Euonymus pauciflorus, Acer triflorum, etc.), 4 taxa of a degree III (Aconitum longecassidatum, Angelica gigas, etc.), 2 taxa of a degree IV (Ulmus macrocarpa and Daphne pseudomezereum var. koreana), 2 taxa of a degree V (Berchemia berchemiaefolia and Alangium platanifolium). The naturalized plants were 5 taxa including Fallopia dumetorum, Trifolium repens, Ailanthus altissima, Oenothera biennis, Erigeron annuus. Naturalization rate (NR) was 1.8% of all 273 taxa of vascular plants and the invasive alien plants were not found. The purpose of this study is to identify the flora that grows in the special protection zones of Songnisan National Park and provide the basic data for the management of the special protection zone in the future.

• Journal of Plant Biotechnology
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• 제42권4호
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• pp.356-363
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• 2015

나무의 유전 공학적 연구를 위해서는 목본 고유의 유전자 및 프로모터 연구가 필수적이다. 우리는 포플러(P. alba ${\times}$ P. glandulosa)의 Pagns-LTP 유전자의 867 bp 프로모터를 분리하였고, ${\beta}$-glucuronidase (GUS) reporter 유전자를 이용한 프로모터의 형질전환 포플러를 제작하여 특성 분석하였다. Pagns-LTP 유전자는 어린뿌리에서 강하게 발현되었고 어린잎에서는 약하게 발현되었으며, 그밖에 다른 조직에서는 발현되지 않았다. 또한, 프로모터의 활성은 뿌리와 어린잎에서 한정되었으며 어린뿌리의 세포 전체에서 강한 활성을 나타내었다. 이에 포플러 ns-LTP 프로모터 내의 cis-element를 조사하고 현사시나무에서 Pagns-LTP 프로모터를 분리한 후 활성을 분석하였다. 프로모터 내의 cis-element를 분석한 결과, 조직 특이적 발현과 호르몬 및 스트레스에 반응하는 다양한 cis-element가 존재함을 확인하였다. 이를 통해 포플러의 ns-LTP는 생장뿐만 아니라, 스트레스에도 관여할 것이라고 추측할 수 있었다. 본 연구는 목본의 유전자 기능 분석 및 다양한 응용 연구를 위해 유용하게 이용될 수 있는 도구로서의 가능성을 제시하였다.

• 기술사
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• 제43권2호
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• pp.45-52
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• 2010

At the end of last year, Korean nuclear power plants, APR-1400 and a research reactor have been contracted to build plants at United Arab Emirates and Jordan. Since 1959, a historical background of nuclear technologies in Korea is summarized. The safety requirements for Instrumentation and Control (I&C) systems in Nuclear Power Plants (NPP) are discussed. Specific descriptions on the typical safety classification of I&C systems, the definitions of the electrical class 1E and the countermeasures against common caused failures are provided. And summaries of typical I&C systems such as the protection systems, the control systems, the instrumentations, the monitoring systems and a control room in NPP are introduced. Strict requirements on the development of the digital computer systems in nuclear applications are described.

• Biomolecules & Therapeutics
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• 제25권4호
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• pp.345-353
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• 2017

Plant expression systems have been developed to produce anti-cancer vaccines. Plants have several advantages as bioreactors for the production of subunit vaccines: they are considered safe, and may be used to produce recombinant proteins at low production cost. However, several technical issues hinder large-scale production of anti-cancer vaccines in plants. The present review covers design strategies to enhance the immunogenicity and therapeutic potency of anti-cancer vaccines, methods to increase vaccine-expressing plant biomass, and challenges facing the production of anti-cancer vaccines in plants. Specifically, the issues such as low expression levels and plant-specific glycosylation are described, along with their potential solutions.

• Journal of Nuclear Fuel Cycle and Waste Technology
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• 제1권1호
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• pp.37-47
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• 2013

This paper describes basic plans for the development of a radioactive waste disposal facility with the introduction of Nuclear Power Plants (NPPs) for Kenya. The specific objective of this study was to estimate the total projected waste volumes of low- and intermediate-level radioactive waste (LILW) expected to be generated from the Kenyan nuclear power programme. The facility is expected to accommodate LILW to be generated from operation and decommissioning of nuclear power plants for a period of 50 years. An on-site storage capacity of 700 $m^3$ at nuclear power plant sites and a final disposal repository facility of more than 7,000 $m^3$ capacity were derived by considering Korean nuclear power programme radioactive waste generation data, including Kori, Hanbit, and APR 1400 nuclear reactor data. The repository program is best suited to be introduced roughly 10 years after reactor operation. This study is important as an initial implementation of a national LILW disposal program for Kenya and other newcomer countries interested in nuclear power technology.

• The Plant Pathology Journal
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• 제39권6호
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• pp.592-599
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• 2023

A defective RNA3 (D3Yα) of strain Y of cucumber mosaic virus (CMV-Y) was examined on host-specific maintenance, experimental conditions, and a viral factor required for its generation in plants. D3Yα was stably maintained in cucumber but not in tomato plants for 28 days post inoculation (dpi). D3Yα was generated in Nicotiana tabacum or N. benthamiana after prolonged infection in the second and the third passages, but not in plants of N. benthamiana grown at low temperature at 28 dpi or infected with CMV-Y mutant that had the 2b gene deleted. Collectively, we suggest that generation and retention of D3Yα depends on potential host plants and experimental conditions, and that the 2b protein has a role for facilitation of generation of D3Yα.

• 무역학회지
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• 제45권5호
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• pp.55-74
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• 2020

This study examines the policy direction and specific countermeasures for addressing possible port labour issues from the perspective of Just Transition which may be raised by closing coal fired power plants in Korea. Current energy transition policy and port labour policy in Korea are reviewed and case studies in the countries which has experienced closure of coal fired power plants are undertaken. Although it varies from country to country, a similar approach was found that the employment problem of coal fired power plant closures and measures based on Just Transition regime to mitigate the negative impacts that occur in the region are the key to successful transition. It is suggested that countermeasures for port labour should be institutionalized for providing stakeholders with legal stability covering labours not only directly employed by the plants but also employed in entities in the whole supply chains.

• The Plant Pathology Journal
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• 제40권3호
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• pp.299-309
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• 2024

The rice blast disease, caused by the fungal pathogen, Magnaporthe oryzae (syn. Pyricularia oryzae), poses a significant threat to the global rice production. Understanding how this disease impacts the plant's microbial communities is crucial for gaining insights into host-pathogen interactions. In this study, we investigated the changes in communities of bacterial and fungal endophytes inhabiting different compartments in healthy and diseased plants. We found that both alpha and beta diversities of endophytic communities do not change significantly by the pathogen infection. Rather, the type of plant compartment appeared to be the main driver of endophytic community structures. Although the overall structure seemed to be consistent between healthy and diseased plants, our analysis of differentially abundant taxa revealed the specific bacterial and fungal operational taxonomic units that exhibited enrichment in the root and leaf compartments of infected plants. These findings suggest that endophyte communities are robust to the changes at the early stage of pathogen infection, and that some of endophytes enriched in infected plants might have roles in the defense against the pathogen.