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http://dx.doi.org/10.5010/JPB.2015.42.4.356

Characterization of a non-specific Lipid Transfer Protein (ns-LTP) promoter from poplar (Populus alba × P. glandulosa)  

Cho, Jin-Seong (Department of Forest Genetic Resources, Korea Forest Research Institute)
Noh, Seol Ah (Department of Forest Genetic Resources, Korea Forest Research Institute)
Choi, Young-Im (Department of Forest Genetic Resources, Korea Forest Research Institute)
Publication Information
Journal of Plant Biotechnology / v.42, no.4, 2015 , pp. 356-363 More about this Journal
Abstract
In order to study genetic engineering in trees, the characterization of genes and promoters from trees is necessary. We isolated the promoter region (867 bp) of Pagns-LTP from poplar (P. alba ${\times}$ P. glandulosa) and characterized its activity in transgenic poplar plants using a ${\beta}$-glucuronidase (GUS) reporter gene. High-level expression of the Pagns-LTP transcript was found in poplar roots, while comparatively low-level expression was found in the young leaves. Pagns-LTP mRNA was not detected in other poplar tissues. Additionally, transgenic poplar plants that contained a Pagns-LTP promoter fused to a GUS reporter gene, displayed tissue-specific GUS enzyme activity localized in root tissue. In silico analysis of the Pagns-LTP promoter sequence reveals the presence of several cis-regulatory elements responsive to phytohormones, biotic and abiotic stresses, as well as those regulating tissue-specific expression. These results demonstrate that the Pagns-LTP promoter has tissue-specific expression activity in poplar roots and leaves that may be involved in organ development and plant resistance to various stresses. Therefore, we anticipate that the Pagns-LTP promoter would be a useful tool to genetically optimize woody plants for functional genomics.
Keywords
Poplar; Promoter-GUS fusion; Non-specific Lipid Transfer Protein; Tissue specific;
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