• 한국패류학회지
• /
• 제31권3호
• /
• pp.249-252
• /
• 2015

An aeolid nudibranch species Flabellina athadona (Bergh, 1875) is newly recorded from Korean waters. This species is distinguished from other congeneric species by the following characteristics: translucent white body, deep orange colored digestive glands, plenty of white specks, and a reversed Y-shaped marking on the head between rhinophores and oral tentacles. In this report, we also provide an identification key for the genus Flabellina in Korea, photographs, and diagnostic characteristics of F. athadona. Molecular identification using mtDNA cox1 partial sequences is also provided to help differentiate F. athadona from some of its closely related congeneric species.

• 대한미생물학회지
• /
• 제35권1호
• /
• pp.69-76
• /
• 2000

Members of the genus Acinetobacter are recognized as newer pathogens of the nosocomial infection with an increasing frequency in recent years. Strains that belonged to A. calcoaceticus A. baumannii complex (genomic species 1, 2, 3, and 13TU) were major groups associated with nosocomial infection. Phenotypic identification was unreliable and laborious method to classify Acinetobacter strains into 19 genomic species. Rapid and reliable identification of clinical isolates is essential to diagnosis and epidemiology of Acinetobacter. We investigated the suitability of amplified ribosomal DNA restriction analysis (ARDRA) to identify genomic species of 131 Acinetobacter isolates. The 16S rRNA genes (ribosomal DNA) were enzymatically amplified and the amplified PCR products were restricted independently with the enzymes, AluI, CfoI, and MboI. Genomic species of Acinetobacter was classified by the combinations of restriction patterns. The analysis was showed that restriction profiles were characteristic for each genomic species. One hundred fourteen isolates were identified as A. baumannii, twelve were identified as genomic species 13TU, and one was identified as genomic species 3. Four isolates were found to be unknown organisms. All of the isolates which were identified to A. baumannii by phenotypic tests were completely discriminated into A. baumannii and genomic species 13TU by ARDRA. This study demonstrates that ARDRA is a rapid and simple techniques for the identification of Acinetobacter species according to the genomic species.

• 미생물학회지
• /
• 제27권4호
• /
• pp.404-414
• /
• 1989

김치발효 중 Gram 양성세균 또는 유산균의 분리와 동정을 시도하였다. 종의 다양성은 분리배지와 온도에 영양을 받았고, 다양성은 온도가 낮아질수록 감소하는 경향이었다. MRS는 세균의 분리에 KM(김치재료로 만든 자연배지)은 종의 수를 파악 하는데 각각 적합하였다. 분리균의 동정은 Bergey's manual of Systematic Bacterio]ogy (1986)를 기초로 하여 작성한 이 분농생표(dichotomous Identification scheme)에 의하여 선행하였다. 각 온도(5, 15, $25^{\circ}C$)에서 동정된 Gram 양성세균은 Leuconostoc 5종, Streptococcus 4종, Pediococcus 3종, Bacillus 2종 그리고 Ltobacillms 18종이였다. 각 온도에서 출현 빈도가 높은 종은 $25^{\circ}C$에서 LactobaIlus ptantanmz, Streptococcµs faffinolactis, Leuconostoc maιnteroid, subsp mlsentιroides 이었고, $15^{\circ}C$에서 L. mesenteroides Lactobacillus fructosus, L. maen teroid,l.I subsp. mesent,roid, 이였고, $5^{\circ}C$ 에서 Leuconostoc sp.(65.2 빈도)에 의하여 이루어졌다. 그리고 각 온도에 따른 김치발효 중 지금까지 알려진 Pediococcus cerevisiae 와 Streptococcus faecalis는 분리되지 않았다.

• Journal of the Korean Wood Science and Technology
• /
• 제44권6호
• /
• pp.897-902
• /
• 2016

This study was conducted to identify wood species from main pillars of Daeungjeon Hall, the main building of Jeonghyesa Temple located in Suncheon, Jeollanamdo Province in Korea. It was known that this temple had been built in the $8^{th}$ century by Hyeso, a national master in the Unified Silla period, and was designated as Treasure No. 804 in 1984 due to its historic and academic values. The results of wood species identification showed that main pillars from Daeungjeon Hall of the Jeonghyesa Temple were made of hard pine, Korean fir (Abies spp.) and Zelkova tree (Zelkova spp.). In addition to the wood species identification, floor plan documentation was also done to help identify what wood species had been used for main pillars. These results could be applied and utilized for the conservation and restoration of Daeungjeon Hall in future. This study may help with the authenticity of conservation works.

• 한국수산과학회지
• /
• 제49권2호
• /
• pp.214-223
• /
• 2016

Joint time-frequency images of the broadband acoustic echoes of six fish species were obtained using the smoothed pseudo-Wigner-Ville distribution (SPWVD). The acoustic features were extracted by changing the sliced window widths and dividing the time window by a 0.02-ms interval and the frequency window by a 20-kHz bandwidth. The 22 spectrum amplitudes obtained in the time and frequency domains of the SPWVD images were fed as input parameters into an artificial neural network (ANN) to verify the effectiveness for species-dependent features related to fish species identification. The results showed that the time-frequency approach improves the extraction of species-specific features for species identification from broadband echoes, compare with time-only or frequency-only features. The ANN classifier based on these acoustic feature components was correct in approximately 74.5% of the test cases. In the future, the identification rate will be improved using time-frequency images with reduced dimensions of the broadband acoustic echoes as input for the ANN classifier.

• 한국식품과학회지
• /
• 제30권3호
• /
• pp.473-479
• /
• 1998

국산 19품종의 현미(40점)를 대상으로 현미 1알로부터 3개의 화상(윗면, 측면, 앞면)의 9개 화상특성(면적, 장단축비, 장축길이, 단축길이, 둘레길이, 원형도, 적색도, 녹색도, 청색도), 도합 27개 화상특성을 측정하여 품종 판별을 시도하였다. 판별식의 도출과 검증을 위하여 현미 시료 1점당 각각 105알과 20알이 사용되었다. 현미의 화상특성 중 윗면 화상, 측면 화상에서의 면적, 장단축비, 장축길이, 단축길이, 둘레, 원형도, 앞면 화상에서의 면적, 둘레는 품종 간 차이가 큰 특성이었다. 27개 화상 특성중 판별에 기여율이 가장 낮은 앞면 화상의 둘레길이, 측면 화상의 원형도, 윗면의 둘레길이를 제외한 24개 화상특성을 사용하였을 때 84.75%로 가장 판별율이 높았다. 품종 판별율은 품종 농안의 경우 최고 99.05%, 화성의 경우 최저 50.63%로 품종 간 차이가 많았다. 품종 판별식을 검증한 결과 평균 84.93%의 품종 판별율을 보였으며, 농안과 팔공의 경우 최고 100%, 화성의 경우 최저 47.62%의 품종 판별율을 보였다. 이 결과, 단지 화상분석에 의한 품종 판별율은 품종에 따라 커다란 차이가 있어 전체 품종 판별에는 적합하지 않은 것으로 사료되었다. 좀더 신뢰성 있는 품종 판별을 위해서는 화상분석 이외에 품종이 지닌 고유의 특성을 분석하는 다른 판별분석법도 병행하여 사용되어야 할 것이다.

• 대한한의학회지
• /
• 제27권2호
• /
• pp.225-231
• /
• 2006

Objectives : This study was performed to determine if unknown species of antler samples could be identified by genetic distance methods. Methods : The DNAs of 4 antler samples were extracted, amplified by PCR, and sequenced. The DNAs of antlers were identified by genetic distance. Genetic distance method was made using MEGA software (Molecular Evolutionary Genetics Analysis, 3.1). Results : By genetic distance methods, all 4 antler samples were closest to Cervus elaphus nelsoni among Cervus species. Conclusion : These results suggest that genetic distance methods might be used as a tool for the identification of Cervus species.

• Fisheries and Aquatic Sciences
• /
• 제11권1호
• /
• pp.32-35
• /
• 2008

The Suminoe oyster, Crassostrea ariakensis, occurs in estuaries where rivers meet seawater. In Korea, it is one of the most popular fisheries resources in the Nam River and Sumjin River. However, the genetic identification of this species has been questioned, because specimens are often mis-identified as other species. To identify the species, we conducted polymerase chain reaction (PCR) amplification of the internal transcribed spacer-1 (ITS-1) region, followed by digestion with the restriction enzyme HaeIII. Restriction profiles for oysters collected from Korea, Japan, and China (north and south) were determined by comparing the PCR-restriction fragment length polymorphism (RFLP) patterns of the ITS-1 regions. Our study verified that the oysters collected from Korea were C. ariakensis based on the PCR-RFLP patterns. These results emphasize the value of molecular markers for identifying morphologically uncertain species.

• 대한미생물학회지
• /
• 제34권6호
• /
• pp.561-571
• /
• 1999

Diagnosis of mycobacterial infection is dependent upon the isolation and identification of causative agents. The procedures involved are time consuming and technically demanding. To improve the laborious identification process mycobacterial systematics supported by gene analysis is feasible, being particularly useful for slowly growing or uncultivable mycobacteria. To complement genetic analysis for the differentiation and identification of mycobacterial species, an alternative marker gene, rpoB encoding the ${\beta}$ subunit of RNA polymerase, was investigated. rpoB DNAs (342 bp) were amplified from 52 reference strains of mycobacteria including Mycobacterium tuberculosis H37Rv (ATCC 27294) and clinical isolates by the PCR. The nucleotide sequences were directly determined (306 bp) and aligned using the multiple alignment algorithm in the MegAlign package (DNASTAR) and MEGA program. A phylogenetic tree was constructed with a neighborhood joining method. Comparative sequence analysis of rpoB DNA provided the basis for species differentiation. By being grouped into species-specific clusters with low sequence divergence among strains belonging to same species, all the clinical isolates could be easily identified. Furthermore RFLP analysis enabled rapid identification of clinical isolates.

• 미생물학회지
• /
• 제19권3호
• /
• pp.128-136
• /
• 1981

In order to investigate early identification of species of the wood-decaying fungi in the the mycelial stage, the authors isolated of 41 species, 28 genus, 9 family at 8 locations in Korea and cultivated these isolates on the various kinds of solid media. After investigating such cultural characteristics as oxidase reactions with tannic and gallic acid, various morphological features of colony and growth grade, appeared on the various media, the authors obtained the following results : 1. The oxidase reactions with tannic and gallic acid in the PDTA, DTA, PDGA and DGA media are available for identificantion of the wood-decaying fungi. 2. The oxidase reactions with guaiacol, pyrogallol and hydroquinone in the PDGUA, PDPA and PDHA media are not so much available for identification of the wood-decaying fungi. 3. Morphological features of colonies such as mycelium color, floccose, floccose-powdery, mycelloid, powdery-mycelloid, velvet, radiate, contoured, rosulate and growth grade on the PDA, PSA and PXA media are useful for identification of wood-decaying fungi. 4. It is believed that early identification in species level of wood-decaying fungi using cultural characteristics in the mycelial stage is possible. 5. The key for the identification of 41 species of wood-decaying fungi is proposed by the cultural characteristics using several solid media.