• Title/Summary/Keyword: SPAWNING SEASON

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Steroid Metabolism in the Blackfin Flounder Glyptocephalus stelleri during Oocyte Maturation (기름가자미(Glyptocephalus stelleri) 성숙기 난모세포에서의 성스테로이드 호르몬 대사물질 분석)

  • Lee, Hae Won;Baek, Hea Ja
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.48 no.4
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    • pp.483-488
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    • 2015
  • We studied oocyte steroidogenesis in the blackfin flounder Glyptocephalus stelleri as a region-specific species, in the East Sea of Korea during the spawning season. Maturing oocytes (0.76, 0.82, 0.88, and 0.91 mm in oocyte diameter) were incubated in vitro in the presence of [$^3H$] $17{\alpha}$-hydroxyprogesterone ($[^3H]17{\alpha}$-OHP) as a precursor. Steroid metabolites were extracted from the incubated medium and oocytes, and the extracts were separated and identified by thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC) and gas chromatographymass spectrometry (GC/MS). The major metabolites produced from $[^3H]17{\alpha}$-OHP were androgens [androstenedione (A4) and testosterone (T)] and estrogens [$17{\beta}$-estradiol (E2) and estrone (E1)] and progestins [$17{\alpha},20{\alpha}$-dihydroxy-4-pregen-3-one ($17{\alpha}20{\alpha}P$) and $17{\alpha}20{\beta}$-dihydroxy-4-pregnen-3-one ($17{\alpha}20{\beta}P$)] in maturing oocytes. The metabolic rate of $17{\alpha}20{\beta}$ was elevated (29.04%) in oocytes measuring 0.88 mm (nucleus migration stage following the induction of germinal vesicle breakdown), but was very low in oocytes measuring 0.76, 0.82, and 0.91 mm (0.42, 0.67, and 2.62%, respectively). From these results, we suggest that $17{\alpha}20{\beta}P$ acts as a maturation-inducing steroid in the blackfin flounder.

Monitoring of Nervous Necrosis Virus (NNV) in the Broodstock of Seven Band Grouper Hyporthodus septemfasciatus (신경괴사증바이러스(Nervous Necrosis Virus, NNV) 모니터링을 통한 무감염 능성어(Hyporthodus septemfasciatus)친어의 선발)

  • Kim, Si-Woo;Kim, Wi-Sik;Seo, Han-Gill;Kim, Kyong Min;Oh, Myung-Joo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.5
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    • pp.527-533
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    • 2017
  • We investigated the infection of nervous necrosis virus (NNV) in seven band grouper Hyporthodus septemfasciatus broodstocks, which have been reared in aquaculture farms in South Korea during 2012-2014. To investigate the prevalence of NNV within the broodstock, egg, sperm, and blood were sampled in the spawning season. The egg and sperm samples were subjected to a nested reverse transcription (RT) polymerase chain reaction (PCR) assay to detect NNV and were inoculated on SSN-1 cells to culture the virus. Blood samples were used to detect antibodies against NNV using enzyme linked immunosorbent assay (ELSIA). Positive values from ELISA were found in 39 of 162 samples (24%) in 2012, and 13 of 28 samples (46%) in 2014. Additionally, 4 of 34 broodstocks (11%) investigated in 2013-2014 were determined to be carriers from the nested RT-PCR and in vitro cultivation. The broodstocks in which antibodies against NNV were detected by ELISA, or in which NNV was detected by the nested RT-PCR assay, posed a risk of vertical transmission of NNV. Therefore, it is necessary to select virus-free broodstocks in seed production to reduce the possibility of the vertical transmission of NNV.

AGE AND GROWTH OF THE YELLOW CROAKER, PSEUDOSCIAENA MANCHURICA JORDAN ET THOMPSON, IN THE WESTERN COASTAL WATERS OF KOREA (한국 서해산 참조기의 연령과 성장)

  • Chung Bang-Chul
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.3 no.3
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    • pp.154-160
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    • 1970
  • The present paper deals with the growth of yellow croaker by scale age reading. This study is based on material from 596 specimens caught by the Danish seine in the Yellow Sea during the period from June 1967 to April 1968. Ring marks of the scale were formed from April to July, corresponding to the spawning season of the fish reported by Bae (1960). Growth rate of each radius of the ring was approximately 0.73. The relationship between the total length and radius of scales, and the relationship between the body weight and total length are represented by the following equations respectively: L=61.350R+50.184 $$W=4.298L^{3.227}\times10^{-3}$$ Maximum total length calculated by the diagram of Walford's growth transformation, $$L_{n+1}=0.6866L_n+10.8730$$, was 346.9mm. Growth curve of the fish can be expressed by the following von Bertalanffy's equation : $$L_t=346.9(1-e^{-0.376(t+0.609)})$$

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Growth and Reproduction of Deep-Water Mud Shrimp (Solenocera melantho) around Geomun Island, Korea (거문도 해역 대롱수염새우(Solencer melantho)의 성자과 성 성숙)

  • OH Taeg Yun;CHOI Jung Hwa;CHA Hyung Kee;KIM Joo Il;KIM Dea Hyun;LEE Ju Hee
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.38 no.4
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    • pp.232-238
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    • 2005
  • This paper describes reproduction, population structure, growth and size at sexual maturity of a deep-water mud shrimp (Solenocera melantho) in the adjacent waters of Geomun Island, Korea. The shrimp was caught by a beam trawl from November 1998 to December 1999. Spawning period was estimated by gonadosomatic index to be from August to early November with a peak in October to early November. Female shrimps were $12\%$ matured at 16 mm in carapace length, $33\%$ matured at 18 mm and $75\%$ matured at 22 mm. The size at $50\%$ maturity for female was estimated to be 20.65 mm in carapace length. Breeding season was estimated to be from August to early November with a peak in October to early November. The size at $50\%$ inseminated was 19.81 mm CL. The fecundity was 87,500-405,200 and related exponentially to carapace length and total weight. This species survived between 25 and 26 months. The maximum carapace length of females and males were 48 mm and 34 mm respectively. Sex ratio was $51.3\%$, and the females were preponderant. Growth was estimated by modified von Bertalanffy growth function incorporating seasonal variation in growth in both years females (K=1.019/yr and $L_{\infty}$=51.731 mm CL) grew faster, reaching a larger size at a given age than males (K=1.848/yr, and $L_{\infty}$=27.499 mm CL). This is indicated by differences in growth performance indices $(\varphi')$ between females (3.291) and males (3.145).

Gonad Maturation Cycle of the Sea Urchin Strongylocentrotus nudus Population Inhabiting an Artificial Seaweed Forest, Samchuk, Korea

  • Kim, Su-Kyoung;Kim, Young-Dae;Jo, Q-Tae;Lee, Jong-Ha;Lee, Chu;Lee, Chae-Sung
    • Fisheries and Aquatic Sciences
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    • v.13 no.2
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    • pp.140-149
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    • 2010
  • We determined the seasonal gonad maturation in Strongylocentrotus nudus sea urchins inhabiting an artificially enhanced seaweed forest along the Samchuk Coast of Korea from April 2006 to March 2007. A total of 30 sea urchins per month were collected from the study area, and gonadosomatic index (GSI), gonad index (GI), egg diameter, and RNA/DNA variation were measured for each specimen. GSI values of female and male urchins achieved maximums of 17.6 and 17.0, respectively, in June. Based on histological studies, maximum GI values occurred in July (4.6 for females and 4.8 for males). A mean ovarian egg diameter of $73.7\;\pm\;14.2\;{\mu}m$ was measured in August; during the main spawning period in September, mean egg diameter reached a maximum of $74.2\;\pm\;17.8\;{\mu}m$. The RNA/DNA ratio and RNA content for both males and females showed a distinct peak during the ripe stage in July, but another peak occurred in the spring season from March to April, when urchins deposit protein into the nutritive phagocytes of immature gonads prior to gametogenesis. The reproductive cycle of S. nudus is divided into five stages: early active (December-May), late active (March-July), ripe (July-September), spent and degenerative (August-November), and inactive (October-February). Our continuous removal of sea urchins from the study area did not influence the reproductive cycle, as populations quickly recovered, and achieved normal gonad development cycle in the site.

Sexual Maturity and Early Life History of the Mudskipper Scartelaos gigas (Pisces, Gobiidae): Implications for Conservation

  • Kim, Jin-Koo;Baek, Hea-Ja;Kim, Jae-Won;Chang, Dae-Soo;Kim, Joo-Il
    • Fisheries and Aquatic Sciences
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    • v.14 no.4
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    • pp.403-410
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    • 2011
  • Scartelaos gigas is an amphibious mudskipper species that inhabits mud flats in Korea, China, and Taiwan. This fish is at risk of extinction because of its very restricted habitat and overexploitation. Information about this fish's reproductive characteristics is needed for species conservation. The sexual maturity and early life history of S. gigas were investigated through histological methods and direct observation of eggs in the wild, respectively. In total, 560 individuals of S. gigas were collected with the aid of fishermen from March 2003 to October 2003 at Jung-do Island, southwest Korea. Through microscopic observations of gonadal development, it was determined that S. gigas of both sexes were immature in April, but began to reach maturity in May, and were then fully mature by June, which was maintained until July. In August, some female fish developed early oocytes, but by September oocytes were observed to have degenerated and had been absorbed. Spawned eggs were elliptical and had an average size of 1.37 mm (long axis) by 0.69 mm (short axis). The newly hatched larvae (3.03 mm total length, TL) had an open mouth and anus, two melanophores near the anus, and one large melanophore between the 18th and 19th myomeres. The larvae (3.18 mm TL) showed absorption of the yolk and oil globule within 5 days after hatching and became prelarvae. This species should be considered vulnerable or conservation-dependent, and thus parental fish need to be protected from fishermen during the main spawning season (June).

A Study on the Testicular Cycle of Korean Brown Frog (Rana coreana) (한국산개구리(Rana coreana) 정소주기에 관한 연구)

  • Shin, Jung-Min;Ko, Sun-Kun
    • Korean Journal of Environmental Biology
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    • v.32 no.2
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    • pp.153-158
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    • 2014
  • In order to determine the testicular cycle of the Korean brown frog, Rana coreana, the gonadosomatic index (GSI) and the changes of germ cells in testis for adult males were investigated throughout the year. The study indicated that the spermatogenesis in the seminiferous tubule of testis began in August and became most active in the month of September, and the GSI was recorded the highest and the cross area of seminiferous tubule was the widest on this period. Furthermore the seminiferous tubules at the post spawning stage appeared in testis during February, and the spermatogenesis was quiescence period of time from March to July and the GSI and the cross area of seminiferous tubule were found to be the lowest. Based on these observations, we suggest that, GSI of male Korean brown frog changes significantly between July to August, indicating the testicular cycle with discontinuous spermatogenic process, and the breeding season was confirmed to be February.

Developmental Duration and Morphology of the Sea Star Asterias amurensis, in Tongyeong, Korea

  • Paik, Sang-Gyu;Park, Heung-Sik;Yi, Soon-Kil;Yun, Sung-Gyu
    • Ocean Science Journal
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    • v.40 no.3
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    • pp.177-182
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    • 2005
  • The process of embryogenesis and larval development of the asteroid sea star Asterias amurensis $(U{\ddot{u}}tken)$ was observed, with special attention paid to morphological change and larval duration. In reproductive season, mature sea stars were collected under floating net cages, located in Tongyeong, southern Korea. The mature eggs are $138\;{\mu}m$ in average diameter, semi-translucent and orange in color, sperms in good condition appear light cream to white-gray in color. Embryos develop through the holoblastic equal cleavage stage and a wrinkled blastula stage that lasts about 9 hours after fertilization. Gastrulae bearing an expanded archenteron hatch from the fertilization envelope 22 hours after fertilization. At the end of gastrulation, rudiments of the left and right coelom are formed. By day 2, larvae possess complete alimentary canal and begin to feed. At this stage, the larva is called early bipinnaria. In 6-day-old larvae, the pre- and post- oral ciliated bands form complete circuits and the bipinnarial processes start to develop. By day 12, the lateral and anterior projection of the larval wall processes along the ciliated bands begins to thicken and curl, and the ciliated bands become more prominent. By day 32, early brachiolaria are presented with three pairs of brachiolar arms. Advanced brachiolaria with a well-developed brachiolar complex (three pairs of brachia and central adhesive disc) occur 6 weeks after fertilization. In the field, spawning of the sea star was observed in April to May, settlement form larvae and just settlements seem to occur from June to July, and early juveniles occur from August to September. Although we had not described the end of brachiolaria stage, it can be tentatively estimated that the duration of the pelagic stage of A. amurensis is 40 to 50 days.

Study on the monthly changes of Perkinsus infection in Komsoe Bay, Chullabukdo, Korea

  • Park, Kwang-Sik;Park, Kyung-Il
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2000.05a
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    • pp.505-505
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    • 2000
  • Perkinsus sp. has been identified as responsible organism for the decrease in Manil clam production along the west and south coast of Korea. Monthly investigation on infection intensity and pathology of Perkinsus infected Manila clam population was carried out in Komsoe Bay located in the west coast during February and December 1999. About one hundred clams were collected each month for the analysis. Infected clams were incubated in fluid thioglycollate media over a week, stained with iodine solution, digested with 2M NaOH and the number of Perkinsus present in an individual recorded. Histological slides were also prepared from infected clams and their pathologic symptoms were examined using a microscope. Trophozoites of Perkinsus sp. were dominantly distributed on gills and epithelia of digestive glands however a few numbers could be detected at siphons and foot tissues. Heavily infected clams often exhibited white spots on mantle and foot tissues due to the inflammatory reaction of the hemocytes, forming nodules. Trophozoites were also found along the connective tissues of follicles during spawning season indicating that Perkinsus sp. may disturb reproduction of the clam. Total number of Perkinsus sp. in an individual clam varied from none to 9, 550, 000 with a monthly mean of 279, 663 to 2, 198, 558 during the course of study. The number of Perkinsus sp. in the clam was found to lowest durin July and August when unusually low salinity was recorded in this area due to the heavy rain. Highest monthly infection intensity in terms of total number of Perkinsus sp. i clam was observed in February, when water temperature recorded as lowest during the study. Small size of clams with shell length of ten mm or less were not infected with Perkinsus sp. It was concluded that Perkinsus infection in Manila clam is in pa controlled by changes in salinity and clam growth; low salinity environment minimize infection intensity while tile clams get more Perkinsus as they grow.

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A Study on the Testicular Cycle of Asian Toad (Bufo gargarizans) (두꺼비(Bufo gargarizans) 정소주기에 관한 연구)

  • Park, Se-Hwa;Ko, Sun-Kun
    • Korean Journal of Environment and Ecology
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    • v.29 no.4
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    • pp.525-532
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    • 2015
  • In order to determine the testicular cycle of the Asian toad, Bufo gargarizans, adult males of the species were captured around Jeongeup city (Jeollabuk-do, Korea) during March, 2012 to February, 2013 and the gonadosomatic index (GSI) and the changes of germ cells in their testes were investigated throughout the year. The study indicated that the spermatogenesis in the seminiferous tubule of testes began in April and became most active in July. The recorded GSI was the highest and the cross area of seminiferous tubule was the widest in this period. The seminiferous tubules at the post spawning stage appeared in February, the largest amounts occurred in March and primary spermatogonia also appeared in this period. The GSI and the cross area of seminiferous tubules were found to be the lowest in March, indicating a testicular cycle with potentially continuous spermatogenic process. According to the findings above, it is confirmed that testicular spermatogenesis takes place actively between April to July in male Asian toad and that their breeding season is February to March.