Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Monitoring of Nervous Necrosis Virus (NNV) in the Broodstock of Seven Band Grouper Hyporthodus septemfasciatus

신경괴사증바이러스(Nervous Necrosis Virus, NNV) 모니터링을 통한 무감염 능성어(Hyporthodus septemfasciatus)친어의 선발

  • Kim, Si-Woo (Department of Aqualife medicine, Chonnam National University) ;
  • Kim, Wi-Sik (Department of Aqualife medicine, Chonnam National University) ;
  • Seo, Han-Gill (Pathology Research Division, National Fisheries Research and Development Institute) ;
  • Kim, Kyong Min (South Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Oh, Myung-Joo (Department of Aqualife medicine, Chonnam National University)
  • 김시우 (전남대학교 수산생명의학과) ;
  • 김위식 (전남대학교 수산생명의학과) ;
  • 서한길 (국립수산과학원 병리연구과) ;
  • 김경민 (국립수산과학원 남해연구소 양식산업과) ;
  • 오명주 (전남대학교 수산생명의학과)
  • Received : 2017.09.25
  • Accepted : 2017.10.23
  • Published : 2017.10.31
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Abstract

We investigated the infection of nervous necrosis virus (NNV) in seven band grouper Hyporthodus septemfasciatus broodstocks, which have been reared in aquaculture farms in South Korea during 2012-2014. To investigate the prevalence of NNV within the broodstock, egg, sperm, and blood were sampled in the spawning season. The egg and sperm samples were subjected to a nested reverse transcription (RT) polymerase chain reaction (PCR) assay to detect NNV and were inoculated on SSN-1 cells to culture the virus. Blood samples were used to detect antibodies against NNV using enzyme linked immunosorbent assay (ELSIA). Positive values from ELISA were found in 39 of 162 samples (24%) in 2012, and 13 of 28 samples (46%) in 2014. Additionally, 4 of 34 broodstocks (11%) investigated in 2013-2014 were determined to be carriers from the nested RT-PCR and in vitro cultivation. The broodstocks in which antibodies against NNV were detected by ELISA, or in which NNV was detected by the nested RT-PCR assay, posed a risk of vertical transmission of NNV. Therefore, it is necessary to select virus-free broodstocks in seed production to reduce the possibility of the vertical transmission of NNV.

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References

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