Korean Journal of Environment and Ecology (한국환경생태학회지)

Korean Society of Environment and Ecology (한국환경생태학회)
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A Study on the Testicular Cycle of Asian Toad (Bufo gargarizans)

두꺼비(Bufo gargarizans) 정소주기에 관한 연구

  • Received : 2015.05.19
  • Accepted : 2015.07.21
  • Published : 2015.08.30
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Abstract

In order to determine the testicular cycle of the Asian toad, Bufo gargarizans, adult males of the species were captured around Jeongeup city (Jeollabuk-do, Korea) during March, 2012 to February, 2013 and the gonadosomatic index (GSI) and the changes of germ cells in their testes were investigated throughout the year. The study indicated that the spermatogenesis in the seminiferous tubule of testes began in April and became most active in July. The recorded GSI was the highest and the cross area of seminiferous tubule was the widest in this period. The seminiferous tubules at the post spawning stage appeared in February, the largest amounts occurred in March and primary spermatogonia also appeared in this period. The GSI and the cross area of seminiferous tubules were found to be the lowest in March, indicating a testicular cycle with potentially continuous spermatogenic process. According to the findings above, it is confirmed that testicular spermatogenesis takes place actively between April to July in male Asian toad and that their breeding season is February to March.

두꺼비(Bufo gargarizans)의 정소주기를 파악하기 위해 2012년 3월부터 2013년 2월까지 전라북도 정읍시 일대에서 채집한 수컷 성체를 대상으로 gonadosomatic index(GSI) 및 정소 내 생식세포의 변화를 연중 조사하였다. 세정관 내 정자형성은 4월부터 시작되어져 7월에 가장 활발하게 진행되었으며 이 시기에 GSI 값이 가장 컸고 세정관 단면적도 가장 넓게 나타났다. 2월부터는 정자배출 후 단계의 세정관이 출현하기 시작하여 3월에 가장 많이 존재하였고 이 시기에 제1 정원세포가 나타났으며 GSI와 세정관 단면적도 최저치를 나타내어 정자형성은 GSI 및 세정관 변화와 일치하는 잠재적 연속형으로 진행되었다. 본 결과들로 보아 두꺼비 수컷의 정소주기 중 정자형성은 4월부터 7월에 활발하게 진행되며 번식기는 2월에서 3월로 확인되었다.

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References

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