• Title/Summary/Keyword: SOD like activity

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Factors affecting to the Quality of Korean Soybean Paste, Doenjang (한국 된장의 품질에 영향을 미치는 요인)

  • Shim, Hye-Jeoung;Yun, Jeong-hyun;Koh, Kyung-Hee
    • Journal of Applied Biological Chemistry
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    • v.61 no.4
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    • pp.357-365
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    • 2018
  • The quality of Korean doenjang, which was traditionally made for this study, was monitored for physicochemical properties, antioxidant capacity, and sensory properties at six months intervals for three years. The collected data were comprehensively analyzed using the k-means clustering via principal component analysis (PCA) to determine the optimal intake duration and sensory factors associated with acceptance. Doenjang samples were classified with every year interval based on PCA, and then the classified doenjang samples were further grouped into cluster one, two, and three based on the k-means clustering. In Cluster three, doenjang that was aged for thirty and thirty-six months, respectively, showed high total phenolic content, antioxidant capacity, superoxide dismutase like activity, and 2,2-diphenyl-1-picryl-hydrazyl radical scavenging capacity. Interestingly, along with acceptance, the levels of free amino acids and organic acids were higher in Cluster 3. The sensory factors found to be associated with acceptance included umami taste and brown color. In conclusion, this study proposes the intake of doenjang aged for thirty months based on its antioxidant activity and sensory properties although doenjang is usually ready after twelve months of aging.

Effect of Brown Rice Flour on Muffin Quality (현미분말 첨가에 의한 머핀의 품질 증진 효과)

  • Jung, Kyong-Im;Cho, Eun-Kyung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.7
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    • pp.986-992
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    • 2011
  • The purpose of this study was to examine the qualities of muffins with 20, 40, and 60% brown rice flour (BRF). The muffins containing 20, 40, and 60% BRF were lower than control muffins (made with regular flour) in weight, height, pH, and moisture content. No significant differences were observed between BRF muffins in weight and moisture content; however, height and pH decreased with increasing amounts of BRF added (p<0.05). The moisture content of control muffins decreased after storage at $30^{\circ}C$ and was not significantly different than BRF muffins (p>0.05). The hardness and resilience in the controls were higher than BRF muffins, but increasing the amount of BRF added in muffins increased both hardness and resilience (p<0.05). The cohesiveness and springiness in the controls were higher than in BRF muffins and decreased with increasing the amount of BRF added to muffins (p<0.05). The gumminess and chewiness in muffins with BRF were higher than controls and were increased when the amount of BRF added in muffins was increased (p<0.05). However, the adhesiveness of the samples was not significantly different (p>0.05). In sensory evaluation, the external color in muffins with 20% BRF and the internal color in controls were the highest (p<0.05). The taste, aroma, texture, and overall acceptability were the highest in muffins with 60% BRF (p<0.05). The antioxidant activities, DPPH radical scavenging, and superoxide dismutase-like activity of muffins were increased with increasing the amount of BRF added to muffins (p<0.05).

Variety, Cutting Date and Physiological Functionality for Production of Leaves in Goji Berry (Lycium chinense Mill.) (구기자 잎 생산에 알맞은 품종, 예취시기 및 생리활성 평가)

  • Ju, Jung-Il;Paik, Seung-Woo;Yun, Tug-Sang;Park, Young-Chun;Lee, Bo-Hee;Son, Seung Wan
    • Korean Journal of Plant Resources
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    • v.33 no.5
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    • pp.436-445
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    • 2020
  • Goji berry (Lycium chinense Mill.) is one of medicinal plants. Its leaves has been used to manufacture the functional foods by replacing the dried-fruit because of low production costs. In order to produce the leaves of uniform quality, continuous selection and the establishment of cultivation techniques were required. Among the eleven recommended cultivars, 'Myeongan' showed high yielding and rapid regeneration after cutting. The dried-leaf yield was linearly increased from 106 kg/10a on May 16 to 287 kg/10a on June 20. An appropriate cutting date and cutting length (about 60-70 cm) was important factors for its efficient regeneration. The late cutting times were not suitable due to difficulties during plant harvesting because of stem rigidity and thorn generation. The betaine content of leaves ranged from 1.43 ~ 2.63% and significantly affected by the varieties and the cutting dates. The main physiological functionality of leaf was Angiotesin-1-converting enzyme (ACE) inhibitory activity, representing the anti-hypertensive. The other physiological functionalities, XOD inhibitory activity, antioxidant activity, tyrosinase inhibitory activity, SOD-like activity, α-glucosidase inhibitory activity and fibrinolytic activity, were not detected or less than 20%.

Antioxidant and Alcohol Dehydrogenase Activity of Water Extracts From Abalone Containing Medicinal Plants (전복과 천연 식물류 복합물의 항산화 및 알코올대사 효소 활성)

  • Shin, Jung-Hye;Lee, Soo-Jung;Choi, Duk-Ju;Kang, Min-Jung;Sung, Nak-Ju
    • Korean journal of food and cookery science
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    • v.24 no.2
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    • pp.182-187
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    • 2008
  • This study was performed to evaluate the possible production of a functional beverage derived from abalone and with several plants (AP). Five types of AP composites were prepared having different medicinal plant compositions (AP- I : abalone, garlic, jujube, Chinese matrimony fruit, dried orange peel, licorice root, zinger, honey; AP- II: abalone, garlic, black bean, Chinese matrimony fruit, cornus fruit, licorice root, zinger, honey; AP-III : abalone, jujube, arrow root, milk vetch, dried orange peel, licorice root, zinger, honey; AP-N: abalone, black bean, arrow root, milk vetch, cornus fruit, liquorice root, zinger, honey; AP-V : abalone, garlic, Chinese matrimony fruit, milk vetch, licorice root, zinger, honey). In vitro analysis were performed to examine the antioxidant contents and alcohol dehydrogenase activities of the composites. AP- II had the highest total phenol contents ($28.55{\pm}1.56\;mg/l00\;g$), and AP- V the highest level of flavonoids ($47.61{\pm}1.58\;mg/l00\;g$). At $78.89{\pm}0.16%$, AP- V displayed the strongest electron donating ability followed by AP-II($57.99{\pm}0.21%$) and API ($37.66{\pm}0.20%$). Reducing power was also significantly higher in AP- V. The Hydroxyl radical scavenging and SOD-like activities of all composites were less than 15% and 20%, respectively. At 12.5% alcohol concentration, ADH activity ranged from $114.47{\pm}2.18{\sim}121.39{\pm}4.36%$ and ALDH activity ranged from $100.04{\pm}2.90{\sim}129.54{\pm}4.80%$; AP- I , AP- II, and AP- Vin 12.5% of alcoholic concentration. The composites of AP- I , AP- II, and AP- V, all containing garlic and Chinese matrimony fruit, were significantly stronger than AP-III and AP-N. Finally, also at 12.5% alcoholic concentration, the ALDH activity of AP- V was higher than its ADH activity.

Effects of Minerals Added to Medicinal Plant Extracts on Alcohol-Induced Oxidative Stress and Alcohol Metabolism in Rats (약용식물 추출물이 첨가된 미네랄이 알코올에 의한 산화적 스트레스 및 숙취해소에 미치는 효과)

  • Lee, Seok-Jun;Kim, Andre;Lee, Jae-Hwa;Kim, Mee-Hee;Lee, Bong-Sang;Jee, Young-Taek;Bin, Jae-Hun;Ha, Jong-Myung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.3
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    • pp.393-400
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    • 2011
  • This study investigates the effects of a hangover beverage (MIX) that contains minerals (highly-salty mineral water, HSMW) and several medicinal plant extracts, on antioxidant and alcohol-metabolizing enzymes in alcohol administered Sprague-Dawley rats. HSMW is pumped from below the sedimentary rock layer of Dadaepo, Busan, South Korea, which is 1,050 m below the land surface; it tastes salty, like sea water. In terms of medicinal plant extracts, the total phenolic and flavonoid contents of Rubus coreanus and Cornus officinalis were measured as being significantly higher than those in Curcuma longa. The results suggest that treatment with MIX significantly increases superoxide dismutase (SOD) activity and DPPH radical scavenging activity. In the 10% HSMW-, for MIX- and company product (CP)-treated groups, the concentration of blood alcohol was significantly reduced 1~5 hr after alcohol loading, compared to that in the control group. In hepatic alcohol-metabolizing enzyme activities, alcohol dehydrogenase (ADH) activity was found to be higher in the MIX- and CP-treated groups than in controls, whereas acetaldehyde dehydrogenase (ALDH) activity was significantly higher in the CP-treated groups than other groups. This study concludes, therefore, that MIX (HSMW) minerals, like as Zn, Ca, Mg, Mn, and others stimulate alcohol-metabolizing enzymes, while the antioxidants of plant extracts prevent the damage otherwise incurred by alcohol toxicity. These results suggest that the hangover beverage (MIX) alleviates alcohol hangover symptoms by stimulating activities related to hepatic alcohol-metabolizing enzymes and antioxidant effects.

Antioxidative Effect on the Green Tea and Puer Tea Extracts (녹차와 보이차 추출물의 항산화 효과)

  • Son Gyu-Mok;Bae Sung-Moon;Chung Ji-Young;Shin Dong-Joo;Sung Tae-Soo
    • The Korean Journal of Food And Nutrition
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    • v.18 no.3
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    • pp.219-224
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    • 2005
  • To see the antioxidative ability of puer tea against green tea, antioxidative effects of water and methanol extracts were compared and researched each. Water extracts from green tea and puer tea contain similar percentage of total polyphenol content while methanol extract from puer tea has about $58\%$ of total polyphenol content of that from green tea. Water extracts from both teas contain similar percentage of the electron donating ability while methanol extract from puer tea has about $54\%$ of the electron donating ability of that from green tea. Solvents made a big difference in SOD-like activities. The activations in water extracts were from $13.46\%$ to $48.93\%$, which didn't give much difference between green tea and puer tea. However, the activations in methanol extracts were $59.63\%$ in green tea and $48.93\%$ in puer tea, which was $3\~4$ times higher than that in water extracts and green tea activated better. The nitrite scavenging activity was similar in water extracts from both teas and the methanol extract from puer tea had a bit higher nitrite scavenging activity than that from green tea. The ACE inhibitory activity in water extract from puer tea was about $38\%$ higher than that from green tea while the activity of methanol extract from green tea was higher than that from puer tea. These results indicate that the antioxidative ability of water extract from puer tea is similar to that from green tea but the ACE inhibitory activity of puer tea reveals higher value and antioxidative ability of methanol extract from puer tea was lower than that from green tea while the nitrite scavenging activity of puer tea was higher.

The Relation of the Elevated Plasma Lipid Levels to Plasma Vitamin E Status and Activities of Erythrocyte Glutathione Perosicase in Smokers (흡연인들에서 증가된 혈장지질 농도가 비타민 E 영양상태와 글루타티온 과산화효 활성에 미치는 영향)

  • 윤군애
    • Journal of Nutrition and Health
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    • v.31 no.8
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    • pp.1254-1262
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    • 1998
  • This study has done to investigate the relationship between the icreased lipid oncentration caused by smoking and plama levels of vitamin A and vitamin E, antiodative enzyme activity, and lipid peroxidation , in 52 male smokers and 32 non-smokers, Dietary vitamin A and vitamin E intake was imilar in both smokers and non-smokers. Absolute plasma concentrations of vitamin A and vitamin E were not significantly different between two groups, whereas vitamin E/cholesterol ration in plasma was low or in smokers than in that of non-smokers(p<0.05). It was considered that this lowered effect was due to the elevated plasma lipid concentration rather than oxidant stress derived from smoking, in view of the fact that smokers had higher cholesterol (15.2%) adn LDL-C(26.6%) levels than non-smokers. In non-smokers, plasma thiobarbiturin acid reactive substances(TBARS) conrrelated positively with total cholesterol(r=0.63466, p<0.001), LDL-C level(r=0.57166, p<0.01) , and LDL-C/HDL-C ratio(r=0.45926, p<0.05) . Activities of glutathione perosidase(GSH-Px) , superoside dismutase(SOD), and catalse made no difference in both groups. However, it was observed in non-smokers that GSH-Px activity had negative correlations with total cholesterol(r=-0.67293, p<0.001), LDL-C level(r=-0.62878, p<0.001), and LDL-C/HDL-C ratio (r=-0.58824, p<0.01), indicating that there was a dependent relationship between lipid perosidation and plasma lipid level. The smokers also showed negative correlations for GSH-Px activity with total cholesterol (r=-0.29946, p<0.05) and LDL-c level (r=0.45914, p<0.001), and LDL-C/HDL-c ratio(r=-0.35438, p<0.05). It seemed that the lipid that the lipid level elevated by sustaines smoking resulted in reducing vitamin E/cholesterol ratio and proportion of antioxidant to oxidant load, and then GSH-Px activity, with insufficient removal of free radicals(TBARS 2.43$\pm$0.51 and 1.81$\pm$0.15nmol/ml in smokers and non-smokers, respectively). These findings suggest that higher plasma lipid levels may play a more important role in perturbing the antioxidant defense system including vitamin E status and GSH-Px activity, at least in circumstances that increase lipid concentration . In addition, in exposure to free radicals like those in cigarette smoke. In those cases the ratio of vitamin E/lipid in plasma can be a more indicator of vitamin E status than plasma levels of vitamin E alone.

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Screening of Anti-wrinkle Resource from Herbal Medicinal Extracts and Stability Test of Its Cosmetic Products (생약재 추출물로부터 주름 개선 소재의 발굴 및 이를 이용한 화장품의 안정성 시험)

  • Cho, Eun-Ah;Cho, Eun-Hye;Choi, Sun-Ju;Park, Keun-Hyoung;Kim, So-Young;Jeong, Yoon-Joo;Ku, Chang-Sub;Ha, Byung-Jhip;Jang, Dong-Il;Chae, Hee-Jeong
    • Korean Journal of Medicinal Crop Science
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    • v.19 no.2
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    • pp.126-135
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    • 2011
  • Various herbal medicinal extracts were examined for the development of cosmetic products with anti-oxidative and anti-wrinkle activity. First, total polyphenol contents and DPPH radical scavenging activities of herbal medicinal extracts were measured. Most herbal samples, except for DW extracts of Portulaca oleracea, Caesalpinia sappan, Taraxacum platycarpum, Carthamus tinctorius, and 70% EtOH extracts of Taraxacum platycarpum and Carthamus tinctorius, showed DPPH radical scavenging activity over 80% at a concentration of $400{\mu}g/m{\ell}$. SOD-like antioxidant activity of DW extracts of Syzygium aromaticum, DW extracts of Eriobtrya japonica and 70% EtOH extracts of Sophora japonica was measured as 40%, 35% and 80%, respectively at a dry matter concentration of $50{\mu}g/m{\ell}$. In elastase inhibition assay, DW extracts of Lycium chinense ($50{\mu}g/m{\ell}$) and 70% EtOH extracts of Areca catechu ($50{\mu}g/m{\ell}$) showed 50% and 40% of inhibition, respectively. At a concentration of $1.250{\mu}g/m{\ell}$, DW extracts of Lycium chinense and 70% EtOH extracts of Areca catechu showed 10% and 30% of collagenase inhibition, respectively. Skin and lotion samples were prepared using the two herbal extracts of high anti-wrinkle activity: Lycium chinense extract and Areca catechu extract. The storage stability of skin and lotion containing each of the selected herbal extracts was evaluated. pH and viscosity were used as stability indicators for the stability test under different storage temperatures and freeze-thaw cycle conditions. The skin and lotion containing each of DW extract of Lycium chinense and 70% EtOH extract of Areca catechu was showed high pH and viscosity stability. The skin and lotion containing DW extracts of Lycium chinense showed relatively higher stability than the skin and lotion containing 70% EtOH extract of Areca catechu, at cycle chamber and freeze-thaw conditions. In summary, these results indicated that cosmetics containing DW extract of Lycium chinense were relatively stable, and this herbal extract could be used as a stable functional cosmetic material.

Antioxidant and Antihypertensive Activities of Solvent Extract from Styela clava Tunic, Fishery Waste (수산폐기물인 미더덕껍질 용매 추출물의 항산화 및 항고혈압 활성)

  • Lee, Sang-Mee;Lee, Ye-Ram;Cho, Kwang-Sik;Park, Soo-Yun;Jang, Eun-Young;Hwang, Dae-Youn;Jung, Young-Jin;Son, Hong-Joo
    • Journal of Environmental Science International
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    • v.24 no.7
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    • pp.917-925
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    • 2015
  • Styela clava tunic is generated in large amounts as a waste from S. clava processing plants and leads to environmental problems. It destroys the beach scenery and causes a bad smell and pollution by trashing on the seashore. Therefore, purpose of this study was to investigate antioxidant and antihypertensive activities of different solvent extracts from S. clava tunic for recycling of fishery waste. Antioxidant and antihypertensive activities of all extracts were concentration-dependent. Of extracts, hot water extract showed the highest DPPH radical scavenging activity with the lowest effective concentration ($EC_{50}$) value (0.733 mg/ml). Chloroform extract exhibited the highest metal chelation activity with the lowest $EC_{50}$ value (2.696 mg/ml). Autoclaved water extract showed the highest NO radical scavenging activity with the lowest $EC_{50}$ value (0.491 mg/ml) and n-hexane extract showed the highest reducing power ($A_{700}=1.897$ at 100 mg/ml). And n-butanol extract showed the highest SOD-like activity with the lowest $EC_{50}$ value (19.116 mg/ml) and ACE inhibition activity with the lowest inhibitory concentration($IC_{50}$) value (0.149 mg/ml). These results indicate that extracts obtained from S. clava tunic may potential candidate to reduce diseases caused by various oxidative stresses and hypertension.

In vivo Antinociceptive Antiinflamatory and Antioxidative Effects of the Leaf and Stem Bark of Kalopanax pictus in Rats (음나무 잎 및 수피의 진통소염효과 및 아주반트로 유발된 산화적 스트레스에 대한 효과)

  • Park, Hee-Juhn;Nam, Jung-Hwan;Jung, Hyun-Ju;Kim, Won-Bae;Park, Kwang-Kyun;Chung, Won-Yoon;Choi, Jong-Won
    • Korean Journal of Pharmacognosy
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    • v.36 no.4 s.143
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    • pp.318-323
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    • 2005
  • The leaves (KPL) of Kalopanax pictus (KP) are used as a vegetable or a functional food in Korean society. The stem bark (Kalopanacis Cortex, KPS) has been traditionally used to treat neurotic pain, rheumatoid arthritis and diabetic disease. This research was undertaken to demonstrate that the leaf extract of KP (KPL) has also the antinociceptive and antiinflammatory effects like the extract (KPS) of Kalopanacis Cortex and to compare the activity levels of several extracts obtained from KP. Antinociceptive and antiinflammatory effects were measured against the extracts described as followings; KPL-1 (the MeOH extract obtained from the leaf shoot of KP collected on May), KPL-2 (the MeOH extract from KP collected on June), KPL-3 (the MeOH ectract from KP with no thorns), KPS-1 (MeOH extract from KPS of a Korean habitat), KPS-2 (MeOH extract from KPS of a Chinese habitat). The antimociceptive test undertaken by acetic acid-induced writhing, hot plate-, and tail-flick methods using mice. The anti-inflammatory test was also undertaken by measuring the edema in the carrageenan-induced test. The order of activity potency in the antinociceptive and antiinflammatory assays was commonly shown as followings: KPL-3>KPS>1>KPS-2>KPL-1>KPL-2. This order was also observed in acetic acid-induced vascular permeability test. The antiinflammatory activity in carrageenan-induced assay was also observed as the following order: KPL-3>KPS- 1>PS-2>KPL-1>KPL-2. In addition, adjuvant-induced rats were used for a model to assess the oxidative stress. Treatment of the rat with the extracts reduced serum thiobarbituric acid-reactive substances (TBARS), hydroxy radical(OH) and superoxide dismutase(SOD) activity caused by FCA together together with the inhibition of hepatic TBARS level and lipofuscin content. The above finding suggests that the leaf extract has the antinociceptive and antinflammatory activity. It is also suggested that KPL-3 with more potent activity than other tested extracts could be developed for a new available biomaterial.