• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.1908-1917
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• 2016

Wild strain L-6 was subjected to combined mutagenesis, including UV irradiation, atmospheric and room temperature plasma, and ion beam implantation, to increase the yield of 1,3-dihydroxyacetone (DHA). With application of a high-throughput screening method, mutant Gluconobacter oxydans I-2-239 with a DHA productivity of 103.5 g/l in flask-shake fermentation was finally obtained with the starting glycerol concentration of 120 g/l, which was 115.7% higher than the wild strain. The cultivation time also decreased from 54 h to 36 h. Compared with the wild strain, a dramatic increase in enzyme activity was observed for the mutant strain, although the increase in biomass was limited. DNA and amino acid sequence alignment revealed 11 nucleotide substitutions and 10 amino acid substitutions between the sldAB of strains L-6 and I-2-239. Simulation of the 3-D structure and prediction of active site residues and PQQ binding site residues suggested that these mutations were mainly related to PQQ binding, which was speculated to be favorable for the catalyzing capacity of glycerol dehydrogenase. RT-qPCR assay indicated that the transcription levels of sldA and sldB in the mutant strain were respectively 4.8-fold and 5.4-fold higher than that in the wild strain, suggesting another possible reason for the increased DHA productivity of the mutant strain.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.24 no.6
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• pp.437-449
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• 1991

Time series of barometrically adjusted sea level at Pusan, Izuhara and Hakada are analyzed to study the fluctuations of the Tsushima Current through the Korea Strait. Variability of sea levels and their differences is divided into two parts with respect to the frequency of 0.01 or 0.02 cycles per day(cpd) At lower frequency, both of sea levels and sea level difference(SLD) are coherent and in phase to each other. Pusan has smaller seasonal variations in sea level than other two stations because the effects of geostrophic current and prevailing wind have a negative influence on the seasonal thermosteric contribution to sea level change. Low frequency variability of SLD thus of the Tsushima Current is much greater in the western channel. For higher frequency parts, SLD in the eastern channel has larger variability and is not coherent with that of the western channel. Sea levels at Pusan and Izuhara are $180^{\circ}$ out of phase with SLD in the western and eastern channel respectively, whereas the Hakada level is in phase. This result indicates that eastern channel has a normal response to the along-channel winds and cross-channel geostrophy because Izuhara faces the eastern channel.

• Molecules and Cells
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• v.25 no.4
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• pp.467-478
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• 2008

Simple sequence repeats (SSRs) and interSSR (ISSR) marker systems were used in this study to reveal genetic changes induced by artificial selection for short/long larval duration in the tropical strain Nistari of the silkworm Bombyx mori. Artificial selection separated longer larval duration (LLD) ($29.428{\pm}0.723days$) and shorter larval duration (SLD) ($22.573{\pm}0.839days$) lines from a base, inbred population of Nistari (larval span of $23.143{\pm}0.35days$). SSR polymorphism was observed between the LLD and SLD lines at one microsatellite locus, Bmsat106 ($CA_7$) and at two loci of 1074 bp and 823 bp generated with the ISSR primer UBC873. Each of these loci was present only in the LLD line. The loci segregated in the third generation of selection and were fixed in opposite directions. In the $F_2$ generation of the $LLD{\times}SLD$ lines, the alleles of Bmsat106 and $UBC873_{1074bp}$ segregated in a 1:1 ratio and the loci were present only in the LLD individuals. $UBC873_{823bp}$ was homozygous. Single factor ANOVA showed a significant association between the segregating loci and longer larval duration. Together, the two alleles contributed to an 18% increase in larval duration. The nucleotide sequences of the $UBC873_{1074bp}$ and $UBC873_{823bp}$ loci had 67% A/T content and consisted of direct, reverse, complementary and palindromic repeats. The repeats appeared to be "nested" (59%) in larger repeats or as clustered elements adjacent to other repeats. Of 203 microsatellites identified, dinucleotides (67.8%) predominated and were rich in A/T and T/A motifs. The sequences of the $UBC873_{1074bp}$ and $UBC873_{823bp}$ loci showed similarity (E = 0.0) to contigs located in Scaffold 010774 and Scaffold 000139, respectively, of the B. mori genome. BLASTN analysis of the $UBC873_{1074bp}$ sequence showed significant homology of (nt.) 45-122 with upstream region of three exons from Bombyx. The complete sequence of this locus showed ~49% nucleotide conservation with transposon 412 of Drosophila melanogaster and the Ikirara insertions of Anopheles gambiae. The A + T richness and lack of coding potential of these small loci, and their absence in the SLD line, reflect the active process of genetic change associated with the switch to short larval duration as an adaptation to the tropics.

• Proceedings of the Korean Vacuum Society Conference
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• 2006.02a
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• pp.217-217
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• 2006

• Proceedings of the Korean Vacuum Society Conference
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• 2007.08a
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• pp.345-345
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• 2007

• The Journal of Korean Academy of Orthopedic Manual Physical Therapy
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• v.22 no.1
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• pp.9-15
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• 2016

Background: This study was conducted to investigate the effects of unstable modified wall squat exercises accompanied by abdominal drawing-in on the gait variables of healthy adults. Methods: The total number of subjects was 30, and 15 were randomly placed in the training group (TG) and 15 in the control group (CG). To determine the gait variables of TG and CG, step length difference (SLD) stance phase difference (STPD), swing phase difference (SWPD), single support difference (SSD), and step time difference (STD) were measured using OptoGait, a gait analysis system. Results: When the pre-intervention and post-intervention results of TG and CG were compared, statistically significant differences in SLD, STPD, SWPD, SSD and STD of TG were seen. Conclusion: Unstable modified wall squat exercises accompanied by abdominal drawing-in might help reduce the deviation between left and right gait variables during walking.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.18 no.6
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• pp.31-36
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• 2004

Optical coherence tomography system has been extensively studied because it has some advantages such as imaging of high resolution, low cost, and compact size configuration. In order to obtain high resolution of OCT system we configured OCT system using a femto-second laser. We measure the pulse width using autocorrelator function because a femto-second laser is ultra short pulse. And we measured the practical resolution using theoretical equation and the measurement of reference sample. It is confirmed that the proposed OCT system has 1.5 times higher resolution and un distinctive cross-sectional image than OCT system with SLD as a light source.

• The Journal of Korean Physical Therapy
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• v.35 no.5
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• pp.125-131
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• 2023

Purpose: This study examined the acute effects of static and dynamic stretching on the flexibility of the hamstring, dynamic balance ability, and function of the lower extremities in healthy adults. Methods: Thirty participants were assigned randomly to three groups: static stretching group (SSG), basic dynamic stretching group (BDSG), and 5 sec dynamic stretching group (5DSG). SSG performed three sets of 30 seconds of static stretching. BDSG performed a single-leg deadlift (SLD) at the same time as SSG, and 5DSG performed SLD held for five seconds. Hamstring flexibility, dynamic balance ability, and lower extremity function were evaluated before and after intervention. Results: The hamstring flexibility significantly improved in all groups (p<0.05). The dynamic balance ability improved significantly after intervention in all groups except BDSG in the anterior direction (p<0.05). There were significant differences in the posterolateral and posteromedial direction in all groups. The function of the lower extremity showed significant improvement over time only in BDSG (p<0.05). Conclusion: This study suggested that basic dynamic stretching and 5 sec dynamic stretching positively affect the hamstring flexibility, dynamic balance ability, and lower extremity function. Therefore, it is recommended to include dynamic stretching in a program for improving the hamstring flexibility, dynamic balance ability, and the lower extremity function. In addition, it is recommended to apply it at different times depending on the purpose.

• Korean Journal of Agricultural and Forest Meteorology
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• v.20 no.3
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• pp.262-276
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• 2018

Models to predict Leaf Wetness Duration (LWD) were evaluated using the observed meteorological and dew data at the 11 citrus orchards in Jeju, South Korea from 2016 to 2017. The sensitivity and the prediction accuracy were evaluated with four models (i.e., Number of Hours of Relative Humidity (NHRH), Classification And Regression Tree/Stepwise Linear Discriminant (CART/SLD), Penman-Monteith (PM), Deep-learning Neural Network (DNN)). The sensitivity of models was evaluated with rainfall and seasonal changes. When the data in rainy days were excluded from the whole data set, the LWD models had smaller average error (Root Mean Square Error (RMSE) about 1.5hours). The seasonal error of the DNN model had the similar magnitude (RMSE about 3 hours) among all seasons excluding winter. The other models had the greatest error in summer (RMSE about 9.6 hours) and the lowest error in winter (RMSE about 3.3 hours). These models were also evaluated by the statistical error analysis method and the regression analysis method of mean squared deviation. The DNN model had the best performance by statistical error whereas the CART/SLD model had the worst prediction accuracy. The Mean Square Deviation (MSD) is a method of analyzing the linearity of a model with three components: squared bias (SB), nonunity slope (NU), and lack of correlation (LC). Better model performance was determined by lower SB and LC and higher NU. The results of MSD analysis indicated that the DNN model would provide the best performance and followed by the PM, the NHRH and the CART/SLD in order. This result suggested that the machine learning model would be useful to improve the accuracy of agricultural information using meteorological data.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.18 no.4
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• pp.34-40
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• 2004

In this paper, we have studied the OCT(Optical Coherence Tomography) system which has been advantages of high resolution, 2-D cross-sectional images, low cost and small size configuration. The characteristics of light source determine the resolution and coherence length. The light source has a commercial SLD with a central wavelength of 1,285 ill11, 35.3 nm(FWHM). The optical delay line is necessary to make equal with the optical path length to scattered light or reflected light from a sample. In order to make equal the optical path length, the stage that is attached to a reference mirror is controled by a step motor. And the interferometer is configured with the Michelson interferometer by using a single mode fiber, and the scanner can be focused on the sample by using a reference ann Also, the 2-dimension cross-sectional images were measured with scanning the transverse direction of the sample by using a step motor. After detecting the internal signal of lateral direction, a scanner is moved to obtain the cross-sectional image of 2-dimension by using step motor. A photodiode, which has high detection sensitivity and excellent noise characteristics has been used. The detected small signal has a noise and interference. After filtering and amplifying the signal, the output signal is demodulated the waveform And then, a cross-sectional image is seen through converting this signal into a digitalized signal by using an AID converter. The resolution of the sample is about 30${\mu}{\textrm}{m}$, which corresponds to the theoretical resolution. Also, the cross-sectional images of onion cells were measured in real time scheme.