• Molecules and Cells
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• 제44권1호
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• pp.38-49
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• 2021

Airway mucus secretion is an essential innate immune response for host protection. However, overproduction and hypersecretion of mucus, mainly composed of the gel-forming MUC5AC protein, are significant risk factors for patients with asthma and chronic obstructive pulmonary disease (COPD). The transforming growth factor β (TGFβ) signaling pathway negatively regulates MUC5AC expression; however, the underlying molecular mechanism is not fully understood. Here, we showed that TGFβ significantly reduces the expression of MUC5AC mRNA and its protein in NCI-H292 cells, a human mucoepidermoid carcinoma cell line. This reduced MUC5AC expression was restored by a TGFβ receptor inhibitor (SB431542), but not by the inhibition of NF-κB (BAY11-7082 or Triptolide) or PI3K (LY294002) activities. TGFβ-activated Smad3 dose-dependently bound to MUC5AC promoter. Notably, TGFβ-activated Smad3 recruited HDAC2 and facilitated nuclear translocation of HDAC2, thereby inducing the deacetylation of NF-κB at K310, which is essential for a reduction in NF-κB transcriptional activity. Both TGFβ-induced nuclear translocation of Smad3/HDAC2 and deacetylation of NF-κB at K310 were suppressed by a Smad3 inhibitor (SIS3). These results suggest that the TGFβ-activated Smad3/HDAC2 complex is an essential negative regulator for MUC5AC expression and an epigenetic regulator for NF-κB acetylation. Therefore, these results collectively suggest that modulation of the TGFβ1/Smad3/HDAC2/NF-κB pathway axis can be a promising way to improve lung function as a treatment strategy for asthma and COPD.

• 한국건강관리협회지
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• 제4권1호
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• pp.75-84
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• 2006

Background : Osteoporosis and atrophic cell pattern in Pap smear are frequent findings In postmenopausal women due to loss of ovarian function, The present study attempted to find out possible correlation between morphologic characteristics of Pap smear and osteoporosis. Material & methods: The subjects were 825 women(age from 35 to 80) who had undergone Pap smear and bone mineral density(BMD) at The Korea Association of Health Promotion, Seoul Branch, from March 8 to May 10, 2005. Pap smears from 825 women were reviewed and classified either mature cell pattern or atrophic cell pattern by their cytologic patterns, BMD were measured using LUNAR DPX MdIQ(Minster, Ohio, USA). BMD value of lumbar spine(Ll, L2,L3 and L4) were measured from 825 women and BMD value of proximal region off emur(neck NK, Wards triangle WT, and trochanter TR) were measured from 818 women and their bone status were classified as normal( T-sore:>-1.0), osteopenia (T-score: -l~<-2,5) and osteoporosis(T-score: ≤ -2.5). And age distribution of Pap smear, average T-value andfrequency ofsteoporo-sis of each region of the bone, percentage of osteoporosis of each boneregion by age group and changing pattern of percentage of osteopenia and osteoporosis in certain postmenopausal period were compared between mature and atrophic cell pattern. Results: Pap smears revealed total mature cell pattern 53,9%(445/825) and total atrophic cell pattern 46.1%(380/825), Percentage of mature cell pattern decreased from 98.2%(168/171)under 44 age group to 13,3%(17/128) over 65 age group and mature cell pattern increased from 1.8%(3/171) under 44 age group to 86.7%(111/128) oyer 65 age group. Mean T-value of each region of lumbar spine and femur of mature cell pattern were lower than that of atrophic cell pattern about -1,5. And osteoporosis has noted in atrophic cell pattern showing odds ratio Ll 13.9, L2 15.3, L3 12.0, L4 10,4, UK 6.7, WT 10.9 and TR 4.1.Atrophic cell pattern started to increase after 45 years of age and osteoporosis of a trophic cell pattern started after 55 years of age. During 50 to 64 years of age period, L3, L4 and WT revealed parallel increased of osteopenia and osteoporosis and Ll, L2 revealed decreased of osteopenia and increased of osteoporosis. nia Conclusion: Above findings suggest that atrophic cell pattern of Pap smear precedes osteoporosis about 10 years and one of predictor of osteoporosis.

• 한국응용곤충학회지
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• 제37권2호
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• pp.187-191
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• 1998

새로운 Bacillus thuringiensis NT0423균주를 이용한 배양체계를 확립하고 대량생산을 위한 새로운 배양배지를 개발하였다 대두박과 밀기울의 다양한 성분비로 구성된 5종의 SW 배지는 기존의 인공합성배지인 GYS나 LB B. huringiensis 의 성장과 포자형성면에서 훨씬 우수한 결과를 보였고, 그중 대두박과 밀기율이 3:2의 비율로 구성된 SW32배지에서 전체 세포수가 3.9$\times$${10}^{8}$CFU/ml에 달했고, 대부분의 세포가 빠르게 포자를 형성하여 (3.7$\times$${10}^{8}$CFU/ml)가장 효율적이었다. 배양에 따른 배지의 pH는 최저 6.2에서 최고 7.3까지 변화하여 성장에 크게 영향을 미치지 않았고, 전체 배양액에서 대두박과 밀기울이 차지하는 비율이 4%일 경우, ml당 4.2$\times$${10}^{8}$CFU/ml의 포자가 형성되어 B. thuringiensis 의 성장에 가장 유리하였다. 교반플라스크와 5ι의 소규모 발효기를 이용한 B. thuringiensis 의 배양조건 확립에 의해 각각 최대 1$\times$${10}^{9}$CFU/ml과 5$\times$${10}^{10}$CFU/ml에 해당하는 많은 양의 균체를 회수할 수 있다.

• 한국항해학회지
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• 제17권3호
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• pp.57-68
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• 1993

The economy of Korea has grown up significantly in its scale. It has, therefore, become imperative to develop countermeasures to prevent work related injuries and occupational illnesses resultining from haza-rdous working conditions and handling harmful substances. A lot of cargo handling accident in port have occurred due to the characteristics of poor working environment, diversity of working place and method, fluctuation of the amount of cargo and handling of heavy, long, harmful and dangerous cargo, etc. According to '91 industrial accident analysis carried out by the ministry of labour, the number of the stevedores injured by cargo handling accident in port were 1, 432 persons (the death accident : 22 per-sons), the amount of industrial accident compensation in port was 6.7 billion won (the amount of economic loss : 33.6 billion won), and the injury occurance rate of the stevedoring industry was higher than that of the whole industry. This paper, therefore, aims to the actual status of the stevedoring industry and to extract the main cau-ses of the accidents related to cargo handling in port through factor analysis using the data of the accide-nts in the whole habour from 1990 to 1992, and to suggest the countermeasures to prevent such accident. The main causes of the accident and countermeasures are found to be as follows through the factor analy-sis : Factor1, factor2, and factor3 related to a defect of human being and management, a defect of state and environment, and an insufficiency of education and law are extracted. The short-term countermeasures to prevent these accidents are 1) to consolidate the safety and health organization in the working spot, 2) to secure a safe condition in working spot before dock work, 3) to strengthen a dock worker's safety educa-tion. The long-term countermeasures are 1) to promote a decasualisation of dock workers, 2) to modernize the cargo working methods through constructing exclusive pier and introducing exclusive cargo handling equipment, 3) to establish a exclusive dock accident prevention organization and the dock workers law. Factor 4, factor5, factor6, and factor7 related to an unfitness, a deficiency of technical knowledge, a nonfu-lfilment of safety measures, and a bad arrangement are extracted. The countermeasures to prevent these accidents are 1) to perform a complete safety inspection of cargo handling equipments and tools and to carry out the dock work according to a working plan, 2) to publish and supply technical safety books, safety instruction book, safety check list, etc., 3) to strengthen the safety patrol at the working spot in habour and to activate a safety fund, 4) to maintain always a clean workshop with the safety consciousness in which the good arragement of the working spot is considered to be the beginning of safety.

• 한국대기환경학회지
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• 제17권5호
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• pp.425-437
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• 2001

This study is based on the uses meta-analysis methodology to examine the statistical consistency and importance of random variation among results of epidemiologic studies between air pollutants exposure and childhood asthma. Studies for this meta-analysis were conducted by reviewing previous results and by asking researcher active in this field for recommendations. Overall, 10 cases of air pollutants exposures and childhood asthma were reviewed. A variety of statistical methods for meta-analysis have been used to assess the combined effects, to identify heterogeneity, and to provide a single summary risk estimate based on a set of simiar epidemiologic studies. In this study, classification of exposure metircs on air environmental epidemiologic studies are reported for (1) aggravation of childhood asthma by a 50 ppb increase SO$_2$(6 individual studies); (2) aggravation of childhood asthma by a 50 ppb increase NO$_2$(5 individual studies); (3) aggravation of childhood asthma by a 50 ppb increase $O_3$(7 individual studies); (4) aggravation of childhood asthma by a 10$\mu\textrm{g}$/m$^3$increase PM$_{10}$ (4 individual studies); (5) aggravation of childhood asthma by a 1 ppm increase CO (2 individual studies); and (6) comparison of results between a Korean study results and this meta-analytic study. Results of this study indicated that an inverse-variance weighted pooling of the hospital admission risk at a 1ppm increment of CO levels was 1.12% (95% CI : 1.01 ~ 1.24). The hospital admission risk was estimated to increase 5% (95% CI : 1.02~1.08), 6%(95% CI : 1.04~1.09), and 5% (95% CI : 1.02~1.09) with each 50ppb increase of SO$_2$, NO$_2$, and $O_3$, respectively. In addition, our results lead to a small but significant elevation in risk of 2% (RR = 1.02, 95% CI = 1.01~1.04) with each 10$\mu\textrm{g}$/m$^3$increase of PM$_{10}$ among 4 individual studies. We found a small elevation in risk of childhood asthma, and pooled results of 10 epidemiologic studies of childhood asthma using increase a cut-off-point levels of air pollutants showed a few pieces of evidence. The results of this meta-analysis suggested that air pollution associated with an increased incidence of childhood asthma. According to this study, relationship between exposure to air pollutants and childhood asthma in Korea seem to be high than results of this meta-analysis.sis.

• 복식
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• 제49권
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• pp.25-48
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• 1999

The purpose of this study is to offer fundamental data for classification of somatotype for boys of elementary school age. The subject were 458 elementary school boys aged from 7 to 12 living in Pusan, Data were collected by 57 anthropometric and 11 photographic measurements and analyzed by factor analysis according to SAS package 1. Through the factor analysis by each period of school ages 6-7 factor were obtained in upper half and they are as followings: 1) Factor 1 is horizontal size of upper half in every period 2) Factor 2 is vertical size of upper half in every period 3) Factor 3 is shoulder shape in the first period and length of upper half in the middle and latter period 4) Facto 4 sis length of upper half in the first period and shoulder shape in the middle and latter period 5) Factor 5 is angle shape of the breast and back in the first period angle shape of the lower breast and back in the middle of period and angle shape of the upper breast and back in the latter of period 6) Factor 6 is angle of shoulder in the first period angle shape of the upper breast and back in the middle of period and angle shape of the lower breast and back in the latter of period 7)Factor 7 is angle of shoulder in the latter of period 2. Through the factor analysis by each period of school ages 5-6 factor were obtained in lower half and they are as followings: 1) factor 1 is horizontal size of upper half in every period 2) Factor 2 is vertical size of upper half in every period 3) Pactor 3 is angle shape of the belly and upper buttock in the first period and length of lower half in the middle and latter period 5) Factor 5 is angle shape of the lower buttock in the first period angle shape of the upper belly and buttock in the middle of period and angle of the side posture in the latter of period 6) Factor 6 is angle shape of the lower buttock in the middle of period and angle shape of the lower belly and buttock

• 한국식품영양학회지
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• 제15권2호
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• pp.104-111
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• 2002

호열균 B. stearotheymophilus으로부터 단백질 고차구조 형성을 촉진하는 내열성 PPIase를 정제하기 위해, 이 균체를 대량으로 배양 집균, 파쇄하여 효소활성을 측정하였다. 효소의 활성측정은 N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide(pAN)를 기질로 사용하였다. chymotrypsin은 기질 이성체(cis-trans 형)의 한쪽(trans)만을 특이적으로 분해하는 반응을 이용하여 PPIase 활성을 측정하였다. 호열균 추출시료로 부터 효소활성을 확인한 후, DEAE-sepharose CL-6B, Sephadex G-75로 정제 후, 최종적으로 Superose TM-12 (FPLC) gel-필트레이션으로 분자량 18kDa의 본 효소를 정제하였다. 정제한 효소의 화학적 특징을 조사한 결과 pH 7.5~8.0사이에 안정하였으며, 최적 pH는 8.0으로 나타났다. 그리고 $65^{\circ}C$에 30분간 열처리 후, 효소활성을 측정한 결과 50%이상의 잔존 활성을 갖는 내열성 효소임을 확인하였다. 정제 단백질의 N-말단 아미노산 분석은 Edman 분해법으로 39 아미노산 잔기를 결정하였다. 그리고 PPIase의 재구성(refolding) 반응은, 요소로 변성시킨 기질 RNase 1을 이용하여 이 단백질의 재구성 (refolding) 실험을 조사한 결과, PPIase는 변성 기질 RNase 1의 재구성(refolding)을 촉진하는데 높은 효과를 가지고 있었다. 호열균 유전자 라이브러리로부터 PPIase 유전자 약 3kb을 클로닝하였다. 재조합 플라스미드 cPI-40에서 프라이머(A-1, B-2)를 이용하여 PPIase N-말단을 코드하는 유전자를 PCR법으로 증폭하여, 염기배열을 결정한 결과 증폭된 단편은 165염기로 형성된 55 아미노산 잔기를 코드하는 open reading frame(ORF)가 연속되고 있었다. 그리고 Edman법으로 결정한 PPIase의 39아미노산 잔기가 이 배열내에 완전히 보존되어 있었다. 이 결과로부터 이 ORF는PPIase구조 유전자의 1/3에 해당하는 단편임을 확인하였다.

• 한국건설순환자원학회논문집
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• 제9권4호
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• pp.561-568
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• 2021

본 연구에서는 타이어 마모성능에 따른 타이어 마모입자 생성량 및 도로변 미세먼지 물질로써 타이어 소재와 도로 포장체 구성 성분의 정량화 분석을 위한 기초적 연구를 수행하였다. 이에, 타이어 트레드 부위 고무의 가황제/가황촉진제의 사용비를 달리하여 고무배합물을 제조하였다. 제조된 고무배합물의 물성 평가 결과 가황제/가황촉진제의 사용비가 증가할수록 가교밀도는 감소하여 마모성능이 불리한 조건임을 확인하였다. 밀폐된 실내 마모시험기에서도 가교밀도가 감소할수록 타이어 마모입자 생성량은 증가하였고, 손실량 100% 대비 84~86%의 타이어 마모입자를 포집할 수 있었다. 타이어 마모입자 중, 96.4~97.7% 분산, 2.3~3.6% 비산되는 것으로 평가되었다. 포집된 타이어 마모입자의 화학분석 결과, 타이어의 마모성능에 따라 타이어 마모입자 내 타이어와 도로 구성 성분 비율(63 : 37 → 75 : 25)이 변화됨을 확인 할 수 있었다. 본 연구에서는 타이어 마모성능에 따라 타이어 마모입자 발생에 대한 영향성을 관찰하기 위하여 실제 도로 현장의 조건 대비 가혹한 실내 마모환경으로부터 실험을 수행하였다. 이에 타이어 마모입자 발생이 도로 포장체 성분보다 더 높은 함량으로 분석되었다. 추후 실제 도로 환경에서 완제품 타이어와 실제 차량을 이용하여 자동차 도로변 미세먼지 저감을 위한 실증화 연구 방법을 도출할 계획이다.

• Child Health Nursing Research
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• 제2권1호
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• pp.69-92
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• 1996

The purpose of study was : 1) to analyze the trend of research on the family with chronically ill children in Korea, 2) to suggest direction for future study on the family with chronically ill children, and contributing to the use of intervention in family nursing practice. Research studies on the family with chronically ill children were selected from the Korean Nusre, the Korean Nurses' Academic Society Journal, and from dissertations, which were conducted between 1975 and 1995. The total numbers of the studies were 35. These studies were analyzed for 1)time of publication or presentation, 2)research design, 3)characteristics of subjects, 4) type of chronic disease, 5)main concepts, 6)measurement tool, 7) the sis for a degree or nondegree, 8) result of correlational studies. The findings of the analysis were as follows : 1) The numbers of studies on the family with chronically ill children have increas rapidly the early 1990's. In research design, the numbers of survey research studies were the highest. Especially, the most frequently research design was the correlational survey. There were 19 correlational studies(25.7%) during the early 1990's. 2) The subjects in 16 studies(45.7%) were mother of chronically ill children and, in 8 studies (22.9% ) were their parents. 3) In most types of chronic diseases, there were 14 hematooncologic disease(32.6%) and 14 hadicapped children (32.6% ). 4) Frequently used research concepts were stress, degree of coping or way of coping, social support, parents' support, family functioning, intensity of family and family adaptation. 5) Acceding to the results of correlational studies, the more family stress was higher the more degree of coping, family functioning, intensity of family and degree of family adaption was lower. The more degree of social support was higher the more stress was lower and degree of coping, family functioning and intensity of family was higher. The more family functioning was higher the more intensity of family and family adaptation was higher. 6) 24 researches on the family with chronically ill children were done for a thesis for a degree and 11 were nondegree research studies. The following suggestions are made based on the above findings : 1) The pattern of these studies related to the family with chronically ill children in domain of Nursing need to be compared with trend in other domains. 2) More replicated research on the family with chronically ill children is needed to develop family nursing intervention and prove the effect of that and more qualitative research on the family with chronically ill children is needed to comprehensive indepth the family with chronically ill children. 3) Further research on the family with chronically ill children is needed to verify subjects and type of chronic disease, develop applicable measurement tools in Korea and identify relation between other concepts. 4) Family nursing researchers should make an effort to apply research result in various clinical settings and community settings, and try to carry out not only team research with clinical nurse but also other multidisciplinary researcher related to the family.

• Journal of Astronomy and Space Sciences
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• 제21권4호
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• pp.481-492
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• 2004

Nb/Al Superconducting Tunnel Junction(STJ) 소자를 제작하여 I-V 특성곡선을 측정하고 제작된 STJ 소자의 초전도체 특성 및 성능 파라미터 값들을 구하였다. 크기가 각각 20, 40, 60, 그리고 $80{\mu}m$인 4종류의 STJ소자를 제작하였으며 각 소자는 총 5층의 Nb/A1/AlOx/Al/Nb 다결정(polycrystalline) 박막으로 구성된 SIS(Superconductor Insulate. Superconductor) 방식의 조셉슨 접합 구조를 갖는다. 이 연구에서 제작한 STJ 소자는 $Tanner^{TM}$ L-Edit 8.3 프로그램으로 설계하였으며 한국표준과학연구원의 SQUID 제조실험실에서 제작하였다. 5층의 STJ 박막은 DC magnetron sputtering, reactive ion etching, CVD(Chemical Vapor Deposition) 장비를 이용해 생성되었다. 제작된 STJ 소자는 액체헬륨으로 냉각(4K)시킨 후 I-V 특성곡선을 측정하여 초전도 특성을 확인하였고, STJ 소자의 성능을 결정하는 파라미터인 energy gap, normal resistance, normal resistivity, dynamic resistance, dynamic resistivity, 그리고 quality factor를 계산하였다. Nb/Al STJ 소자의 FWHM 에너지 분해능 계산 결과, 순수 Nb STJ 소자보다 $11\%$ 우수한 에너지 분해능 특성을 확인하였다.