• 한국자원식물학회지
• /
• 제25권4호
• /
• pp.407-415
• /
• 2012

눈잣나무는 동북아시아가 주 분포지로 남한에서는 설악산 고산지역에만 제한적으로 분포한다. 본 연구는 설악산 눈잣나무 집단의 분포형태와 특성, 유전다양성 및 공간분포에 따른 유전구조를 파악하였다. 선발된 9개 I-SSR primer에서 총 78개 I-SSR 증폭산물을 얻었으며, 30개의 단형성 증폭산물을 제외한 48개의 증폭산물을 분석에 이용하였다. 조사구(40 m ${\times}$ 70 m)에는 눈잣나무 65개체가 자생하고 있었으며, 채집한 눈잣나무의 위치자료를 바탕으로 군집지수를 계산한 결과 약하게 집중분포(Aggregation Index = 0.871)하고 있음을 확인하였다. 모든 개체에 대하여 I-SSR 유전자형을 비교한 결과, 65개체 중 유전자형이 서로 다른 40개의 genet이 식별되었다. 유전자형 비율(G/N)은 61.5%, 유전자형 다양성(D)은 0.977, 유전자형 균등도(E)는 0.909로 각각 나타났다. Shannon의 다양성지수(I = 0.567)는 적은 개체수와 제한적 분포에도 불구하고 다른 수종들에 비해 비교적 높은 유전다양성을 나타났다. 공간적 자기상관 분석을 실시한 결과 조사지역 내의 눈잣나무 집단은 12 m 이내에서 유전적으로 유사한 군락구조를 갖고 있는 것으로 나타났다. Mantel 검정 결과 유전적 거리와 지리적 거리간에 낮은 상관관계를 나타내 눈잣나무 집단이 초기에 여러 개의 모수에서 형성된 것으로 추정되었다. 본 연구결과 설악산 눈잣나무 집단의 현지외 유전자 보존을 위한 표본추출 전략은 최소 12 m 이상의 거리를 두는 것이 효율적인 것으로 나타났다.

• 한국잡초학회지
• /
• 제8권1호
• /
• pp.1-8
• /
• 1988

본(本) 연구(硏究)는 원예작물(園藝作物) 경작지(耕作地)에서 잡초방제방법(雜草防除方法)을 확립(確立)하는데 필요(必要)한 기초자료(基礎資料)를 얻고저 마늘은 월동전(越冬前)인 1984년(年)과 월동후(越冬後)인 1985년(年), 고추는 1985년(年)에 천원(天原), 아산(牙山), 서천(舒川), 유성(儒城) 4개(個) 지역(地域)에서 2개(個) 지점(地點)을 선정(選定)하여 약(約) 15일(日) 간격(間隔)으로 잡초(雜草)를 채취(採取)하여 잡초수(雜草數), 건물중(乾物重), 발생잡초(發生雜草)의 종생태적(種生態的) 및 사회적(社會的) 특성(特性) 분석(分析)과 우점도(優占度)를 구명(究明)하였다. 1. 마늘경작지(耕作地)에 발생(發生)하는 초종(草種)은 27과(科) 68종(種)이었으며 우점초종(優占草種)은 쇠비름, 명아주, 바랭이였고 차우점초종(次優占草種)은 쇠뜨기, 둑새풀, 강아지풀이었으며 발생잡초(發生雜草)의 본수(本數)는 5월(月), 건물종(乾物種)은 6월(月)에 가장 많았고 마늘재배기간(栽培期間) 동안 여름 광엽잡초(廣葉雜草)가 가장 많이 발생(發生)하였다. 2. 고추경작지(耕作地)에서는 17과(科) 38종(種)의 잡초(雜草)가 발생(發生)했으며 바랭이, 방동사니, 쇠비름이 우점(優占)하였고 피, 왕바랭이, 민바랭이, 중대가리풀 등이 차우점(次優占)했으며 발생잡초(發生雜草)의 본수(本數)는 6월(月)에 많았고 건물중(乾物重)은 6월(月)과 10월(月)에 낮았던 것을 제외하면 재배기간(栽培期間) 동안 큰 차이가 없었으며 여름 화본과(禾本科)와 여름 광엽잡초(廣葉雜草)는 6, 7월(月)에 발생(發生)이 많았다. 3. 고추경작지(耕作地), 마늘경작지(耕作地)에서 시기별(時期別) 종다양도(種多樣度), 최대종다양도(最大種多樣度)와 안정도(安定度)가 높아 발생초종(發生草種)이 많은 숙전(熟田)이었으며 Simpson 지수(指數)도 높아 몇 초종(草種)이 우점(優占)하는 군락(群落)이었고 종간경쟁(種間競爭)이 종내경쟁(種內競爭)보다 높아 초종간(草種間) 경쟁(競爭)이 많았다.

• 한국조경학회지
• /
• 제28권6호
• /
• pp.62-76
• /
• 2001

This study is to offer the basic information and materials for the restoration of the urban forest and the ecologically healthy and continuous green belt through the investigation and the analysis on the vegetation in Hwasan Park, Chonju-City. The flora of Hwasan Neighborhood park was composed of 86 families 230 genera, 261 species, 35 varieties, 1 formae or 298 Taxa. Among them, calculation of Pte-Q Index represented 0.60, Fisher's Index. Some characteristics of the plants in this area have represented such as Th-D1-R5 type in biological type, erect form(64.6%) in growth type, 30 taxa in naturalized plants. The life form spectra investigated from Hwasan neighborhood park was recognized as the following M"17.4%, N:11.4%, E:0.3%, Ch:0%, H:29.8%, G:6.7%, HH:0%, Th:34.2%. By the Z-M method 5 plant communities and 3 afforestation were recognized; Quercus variabilis community, Q.acutissima community, Q.serrata community, Sorbus alnifolia community, Pinus densiflora community, P.rigida afforestation, Robinia pseudo-acacia afforestation and Larix leptolepis afforestation. The actual vegetation map constructed on the grounds of the communities classified and other data. The pattern of successional trend of tree species might be suggested: L. leptoleipis, P. rigida and P. densifloralongrightarrowQ.variabilis and Q.acutissimalongrightarrowS.alnifolia and Q.serratalongrightarrowCarpinus tschonoskii community. Three measurements of species diversity (richness index(SR), Shannon-Wiener index(H'), evenness index(J')) and their relationship with community type were studied in the surveyed plant communities. Q.serrata community was higher in SR, H', J' than the other communities, whereas R.pseduo-acacia afforestation was low. Finally, The vegetation of the Hwasan neighborhood park must not allowed any more. Nothing ca be better than native states in preserving the ecosystems.tems.

• 미생물학회지
• /
• 제30권6호
• /
• pp.444-449
• /
• 1992

낙동강 하구생태계에 분포한 종속영양세균의 환경 변화에 따른 군집구조의 변화를 파악하기 위해 총 858 균주를 분리하여 수리학적 방법에 의해 분석하였다. 종의 다양성(H') 은 1.54-3.49 로, 하구언 건설 전의 수치와 큰 차이가 없었으나, 균등성지수(evenness index)(J') 는 0.31-0.80 으로 감소하였다. Physiological tolerance index 중 수온에 대한 지수(P') 는 수심이 얕은 정점에서, pH 와 염분에 대한 $P_{h}$ 및 $P_{s}$ 는 담수와 해수가 교차하는 정점에서 높았다. 우점의 cluster 를 속 (genus) 수준에서 동정한 결과 Aeromonas, Vibrio, Pseudomonas, Acinetobacter-Morexella, Alcaligenes, Flavobacterium, Micrococcaceae, Enterobacteriaceae 등으로 분류되어 하구언 축조 전과 동일한 종류의 세균뷴포를 나타냈으나 그 출현비율은 달랐다. 이와 같은 결과로 미루어 하구언 축조 등에 따른 낙동강 하구역의 환경변화는 분포세균의 종류보다는 생리적 특셩에 영양을 미쳐 특정종의 우점을 나타나게 한 것으로 사료된다.

• Journal of Microbiology and Biotechnology
• /
• 제31권2호
• /
• pp.181-188
• /
• 2021

Bacillus subtilis and Enterococcus faecium are commonly used probiotics. This study aimed to identify the effect of live combined Bacillus subtilis R0179 and Enterococcus faecium R0026 (LCBE) on obesity-associated hyperlipidemia and gut microbiota in C57BL/6 mice. Forty male C57BL/6 mice were divided into four groups: normal group (N group), model group (M group), low-dose group (L group), and high-dose group (H group). Mice were gavaged with LCBE at 0.023 g/mice/day (L group) or 0.23 g/mice/day (H group) and fed with a high-fat diet for 8 weeks. In vitro E. faecium R0026 showed an ability to lower the low-concentration of cholesterol by 46%, and the ability to lower the high-concentration of cholesterol by 58%. LCBE significantly reduced the body weight gain, Lee index, brown fat index and body mass index of mice on a high-fat diet. Moreover, LCBE markedly improved serum lipids (including serum triglyceride, total cholesterol, low-density lipoprotein and high-density lipoprotein) while also significantly reducing liver total cholesterol. Serum lipopolysaccharide and total bile acid in L and H groups decreased significantly compared with M group. PCR-DGGE analysis showed that the composition of gut microbiota in the treatment groups was improved. Akkermansia muciniphila was found in H group. The PCA result indicated a similar gut microbiota structure between LCBE treatment groups and normal group while the number of bands and Shannon diversity index increased significantly in the LCBE treatment groups. Finally, qPCR showed Bifidobacterium spp. increased significantly in H group compared with M group, LCBE alleviated liver steatosis and improved brown adipose tissue index.

• 한국자원식물학회지
• /
• 제15권2호
• /
• pp.96-106
• /
• 2002

자생 배나무속 유실수 식물유전자원으로 가치가 높은 취앙네의 대덕산 자생지 일대 5 지점을 조사하였다. 취앙네는 해발 1246-1310ｍ의 햇볕이 잘 드는 산 정상 혹은 능선부의 남서사면에서 주로 조사되었다. 자생지 식생에서 총40과 79속 83종 12변종 1품종의 식물들이 조사되었으며 호광성 식물인 국화과의 각시취, 수리취 등을 비롯하여 장미과의 양지꽃 등이 널리 분포하였다. 자생지에서 취앙네는 신갈나무와 거의 비슷한 세력으로 혼재하여 출현하였다. 터주형 생태전략 식물의 차지하는 비율이 54.17%로 나타났다. 취앙네의 자생지는 산불을 비롯한 교란행위에 의해 다양한 식물 종군의 2차 천이가 활발하게 진행중인 지역으로 Shannon-Weiner의 종다양도 지수는 4.592-5.270의 범위로 나타났다. 대덕산 일대에 자생하는 취앙네의 29%는 정상적인 생육을 유지하고 있으나 57%는 생육이 불량하거나 보통인 상태로 조사되었다. 그러므로 취앙네의 생육에 방해를 줄수 있는 덩굴성 식물 및 교목류들의 정기적인 제거작업에 의해 수관부의 수광량 확보와 종간의 경쟁을 피할 수 있는 조치가 필요하다고 사료되는 바이다.

• The Plant Pathology Journal
• /
• 제37권6호
• /
• pp.521-532
• /
• 2021

Knowledge and better understanding of functions of the microbial community are pivotal for crop management. This study was conducted to study bacterial structures including Acidovorax species community structures and diversity from the watermelon cultivated soils in different regions of South Korea. In this study, soil samples were collected from watermelon cultivation areas from various places of South Korea and microbiome analysis was performed to analyze bacterial communities including Acidovorax species community. Next generation sequencing (NGS) was performed by extracting genomic DNA from 92 soil samples from 8 different provinces using a fast genomic DNA extraction kit. NGS data analysis results revealed that, total, 39,367 operational taxonomic unit (OTU), were obtained. NGS data results revealed that, most dominant phylum in all the soil samples was Proteobacteria (37.3%). In addition, most abundant genus was Acidobacterium (1.8%) in all the samples. In order to analyze species diversity among the collected soil samples, OTUs, community diversity, and Shannon index were measured. Shannon (9.297) and inverse Simpson (0.996) were found to have the highest diversity scores in the greenhouse soil sample of Gyeonggi-do province (GG4). Results from NGS sequencing suggest that, most of the soil samples consists of similar trend of bacterial community and diversity. Environmental factors play a key role in shaping the bacterial community and diversity. In order to address this statement, further correlation analysis between soil physical and chemical parameters with dominant bacterial community will be carried out to observe their interactions.

• Journal of Forest and Environmental Science
• /
• 제31권3호
• /
• pp.149-163
• /
• 2015

The aim of the present study was to assess the variation in species composition and diversity of woody species at different altitudes (900 to 2600 m asl) in Kedarnath Wildlife Sanctuary (KWLS) and its adjoining areas in Garhwal Himalaya, India. A total of 94 woody plant species (including 44 tree and 50 shrub species) belonging to 72 genera and 44 families were reported. Density varied from $235{\pm}9$ to $505{\pm}21trees\;ha^{-1}$ and $4,730{\pm}474$ to $9,530{\pm}700shrubs\;ha^{-1}$. Total basal cover varied from $10.49{\pm}0.66$ to $42.92{\pm}2.57m^2ha^{-1}$ (trees) and $0.36{\pm}0.024$ to $0.62{\pm}0.047m^2ha^{-1}$ (shrubs). Shannon-Wiener Index fluctuated between 2.30 to 3.53 (trees) and 2.74 to 3.78 (shrubs). Analysis of variance (ANOVA) indicated that altitude and aspect had significant effect on the distribution of woody species. Taxonomically, Rosaceae with 15 species emerged as the dominant family. Low value of maturity index and contiguous distribution of species denoted the early successional status of the studied forests. The conservation assessment based on altitudinal regimes and the information on species structure and function can provide baseline information for monitoring and sustaining the biodiversity.

• Plant Resources
• /
• 제4권3호
• /
• pp.130-139
• /
• 2001

Four major naturally populated areas around the Jindong valley in Inje-Gun, Gangwon province for wild Pyrus ussuriensis, Malus sieboldii, and Malus baccata are mostly located on the southeast side of the mountains at 630-745m in altitude at sea level and are in very good sunny areas. The total of 77 taxa dividing into 32 families, 58 genera, 65 species, and 12 varieties has been inhabited in these areas. Most of these plants are heliophytes, which love sunshine, but some of rhizo-plants such as Sasa borealis, climbing plants such as Actinidia arguta and Celastrus orbiculatus, or naturalized plants like Bidens frondosa are also shown. These areas have been continually destroyed, so it is possibly thought that the second transition has been progressed. The index of species diversity of plant groups in these areas is 0.672∼1.465 based on the Shannon-Wiener's method, but its index for the area that rhizo-plants like Sasa borealis are growing as an under planting decreases relatively. In this study, the oldest plant of Pyrus ussuriensis in Korea, which is 120cm in diameter at breast height (DBH), 19m in tree height and 25m in width, has been found.

• 한국환경복원기술학회지
• /
• 제10권6호
• /
• pp.120-129
• /
• 2007

Rapid urbanization has transformed the spatial pattern of urban land use or cover. This paper concentrates that changed characteristics of landscape structure in the Tancheon Watershed, from 1995 to 2003 were investigated using land cover map. We used FRAGSTATS software to calculate landscape indices to characterize the landscape structure. We found that built up area has been increased rapidly during the study period, while cultivated area and forest area have been decreased rapidly in the same period. From 1995 to 2003, built up area was increased from 19.73% to 39.62% and cultivated area and forest area was decreased 17.60% to 5.97% and 58.31% to 49.41%. Number of patches, mean euclidean nearest-neighbor distance, contagion index, Shannon's diversity index increased considerably from 1995 to 2003, also suggesting the landscape in the study area became more fragmented and heterogeneous. but because of continuously fragmentation, landscape became homogeneity. The study demonstrates that landscape metrics can be a useful indicator in landscape monitoring and landscape assessment.