• Journal of the Korea Concrete Institute
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• v.26 no.5
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• pp.651-660
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• 2014

Structural members using ultra high strength concrete which usually used with steel fiber is designed with guidelines based on several investigation of SF-RPC(steel fiber reinforced reactive powder concrete). However, there are not clear design method yet. Especially, SF-RPC member should be casted with steam(90 degree delicious) and members with SF-RPC usually used with precast members. Although the most important design parameter is development method between SF-RPC and steel reinforcement(rebar), there are no clear design method in the SF-RPC member design guidelines. There are many controversial problems on safety and economy. Therefore, in order to make design more optimum safe design, in this study, we investigated bond stress between steel rebar and SF-RPC according to test. Test results were compared with previously suggested analysis method. Test was carried out with direct pull out test using variables of compressive strength of concrete, concrete cover and inclusion ratio of steel fiber. According to test results, bond stress between steel rebar and SF-RPC increased with increase of compressive strength of concrete and concrete cover. Increasing rate of bond stress were decrease with increase of compressive strength of SF-RPC and concrete cover significantly. 1% volume fraction inclusion of steel fiber increase the bond stress between steel rebar and SF-RPC with two times but 2% volume fraction cannot affect the bond stress significantly. There are no exact or empirical equations for evaluation of SF-RPC bond stress. In order to make safe bond design of SF-RPC precast members, previously suggested analysis method for bond stress by Tepfers were evaluated. This method have shown good agreement with test results, especially for steel fiber reinforced RPC.

• Journal of Life Science
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• v.29 no.2
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• pp.256-264
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• 2019

Lymphotoxin ${\beta}$ receptor ($LT{\beta}R$), a member of the tumor necrosis factor receptor family, plays an important role in lymphoid tissue's architecture and organogenesis. In contrast, MLCK and ROCK play critical roles in the regulation of stress fiber (SF) formation in cells. To determine whether $LT{\beta}R$ stimulation in fibroblastic reticular cells (FRCs) is involved in these signaling pathways, myosin light chain kinase inhibitor-7 (ML-7) was used to inhibit them. ML7-treated FRCs completely blocked SFs and showed retraction and shrinkage processes comparable to those observed in agonistic anti-$LT{\beta}R$ antibody-treated cells. The inhibition of ROCK activity with Y27632-induced changes in actin cytoskeleton organization and cell morphology in FRCs. Actin bundles rearranged into SFs, and phospho-myosin light chain (p-MLC) co-localized in FRCs. We checked the level of Rho-guanosine diphosphate (RhoGDP)/guanosine triphosphate (GTP) exchange activity using FRC lysate. When $LT{\beta}R$ was stimulated with agonistic anti-$LT{\beta}R$ antibodies, Rho-GDP/GTP exchange activity was markedly reduced. Regarding $LT{\beta}R$ signaling with a focus on MLCK inhibition, we showed that the phosphorylation of MLCs was reduced by $LT{\beta}R$ stimulation in FRCs. Cytoskeleton components, such as tubulin, b-actin, and phospho-ezrin proteins acting as membrane-cytoskeleton linkers, were produced in de-phosphorylation, and they reduced expression in agonistic anti-$LT{\beta}R$ antibody-treated FRCs. Collectively, the results suggested that MLCK and ROCK were simultaneously responsible for SF regulation triggered by $LT{\beta}R$ signaling in FRCs.

• Journal of The Korean Society of Agricultural Engineers
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• v.56 no.5
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• pp.45-54
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• 2014

This study was performed to evaluate compressive strength, flexural strength, static modulus of elasticity, stress-strain ratio and durability of abrasion on EVA concrete reinforced steel fiber (SF) in order to use hydraulic structures, underground utilities, offshore structures and structures being applied soil contaminated area. It is used ordinary portland cement, crushed coarse aggregate, nature fine aggregate, EVA redispersible polymer powder, superplasticizer and deforming agent to find optimum mix design of EVA concrete reinforced steel fiber. EVA concrete reinforced SF was effected on the improvement of mechanical properties and durability of abrasion.

• Journal of Life Science
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• v.27 no.10
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• pp.1199-1206
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• 2017

The lymphotoxin ${\beta}$ receptor ($LT{\beta}R$), a member of the tumor necrosis factor receptor family, plays an important role in lymphoid tissue's architecture and organogenesis. We found that $LT{\beta}R$ stimulation induced changes in stress fibers (SFs) in fibroblastic reticular cells (FRCs). MLCK and ROCK play critical roles in the regulation of SF formation in cells. The present study was performed to investigate the antifibrotic effects on SF regulation of $LT{\beta}R$ signaling, with a focus on MLCK inhibition. The effect of $LT{\beta}R$ on the SF change was analyzed using immunoblot and fluorescence assays and agonistic $anti-LT{\beta}R$ antibody-treated FRCs. In addition, we checked the level of Rho-guanosine diphosphate (GDP)/guanosine triphosphate (GTP) exchange activity with FRC lysate. Phospho-ezrin proteins acting as membrane-cytoskeleton linkers completely de-phosphorylated in agonistic $anti-LT{\beta}R$ antibody-treated FRCs. The actin bundles rearranged into SFs, where phospho-myosin light chain (p-MLC) co-localized in FRCs. ML7-treated FRCs completely blocked SFs and showed retraction and shrinkage processes comparable to those observed in agonistic $anti-LT{\beta}R$ antibody-treated cells. Inhibition of ROCK activity induced changes in the actin cytoskeleton organization; however, some SFs remained in the cells, while they were completely disrupted by MLCK inhibition with ML7. We showed that the phosphorylation of MLC was completely abolished with $LT{\beta}R$ stimulation in FRCs. When $LT{\beta}R$ was stimulated with the agonistic $anti-LT{\beta}R$ antibody, the Rho-GDP/GTP exchange activity was reduced, however, the activity was not completely abolished. Collectively, the results illustrated that MLCK was potently responsible for the SF regulation triggered via $LT{\beta}R$ signaling in FRCs.

• Journal of Life Science
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• v.25 no.5
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• pp.585-593
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• 2015

Stress fiber (SF) alteration is mediated by cellular receptors, which, upon interaction with the extracellular counterpart, signal to the actin cytoskeleton for remodeling. This association is mediated by a variety of scaffold and signaling factors, which control the mechanical and signaling activities of the interaction site. The heterotrimeric transmembrane lymphotoxin α1β2 (LTα1β2), a member of the tumor necrosis factor (TNF) family of cytokines, including soluble homotrimeric lymphotoxin (LT α), plays an important role in lymphoid tissue architecture. Ligation between LTα1β2 and the lymphotoxin β receptor (LTβR) activates signal-cascade in fibroblastic reticular cells (FRCs). We found LTβR stimulation using an agonistic anti-LTβR antibody alone or combined with LTα or TNFα induced changes in the actin and plasticity of cells. To clarify the involvement of myosin underlying the alteration, we analyzed the effect of myosin light chain kinase (MLCK) with an MLCK inhibitor (ML7), the phosphorylation level of myosin light chains (MLC), and the level of phospho-myosin phosphatase target subunit 1 (MYPT1) after treatment with an agonistic anti-LTβR antibody for cytoskeleton reorganization in FRCs. The inhibition of MLCK activity induced changes in the actin cytoskeleton organization and cell morphology in FRC. In addition, we showed the phosphorylation of MLC and MYPT1 was reduced by LTβR stimulation in cells. A DNA chip revealed the LTβR stimulation of FRC down-regulated transcripts of myosin and actin components. Collectively, these results suggest LTβR stimulation is linked to myosin regarding SF alteration in FRC.

• Journal of the Korean Recycled Construction Resources Institute
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• v.2 no.3
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• pp.188-195
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• 2014

This paper was evaluated the rheology properties according to each step of paste, mortar and HPFRCC as a part of the basic study to development of sprayed high performance fiber reinforced cementitious composites(HPFRCC) for protection and blast resistant. Rheology test results in step of paste, in case of GGBFs and FA, it showed that the plastic viscosity and yield stress reduced gradually according to the increase of mixing rate, and in case of SF, the plastic viscosity and yield stress increased radically starting from the mixing rate of 10%. Rheology test results in step of mortar, type of aggregates, it showed that particle shape and grading of aggregate is influence on plastic viscosity and yield stress, and change of volume ratio is influence on plastic viscosity than yield stress. Fluidity and rheology test results in step of HPFRCC, if after a fiber mixed, it showed that viscosity agent is more effective to improve the fluidity and fiber dispersion than superplasticizer.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2014.11a
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• pp.186-187
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• 2014

Flexural stress and fracture energy of fiber reinforced cementitious composites is increased by bridge effect of reinforced fiber, scabbing failure is restrained. Shape, properties of fiber were SF(steel fiber), PA(polyamide), NY(nylon) have effects on flexural stress and fracture energy, impact resistance improve of fiber reinforced cementitious composites. In this study, local failure properties by impact of high velocity projectile was analyzed by mixing 3 types of fiber which have different shape and properties respectively.

• Steel and Composite Structures
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• v.51 no.3
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• pp.243-260
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• 2024

Thirteen self-compacting recycled concrete filled aluminium tubular (SCRCFAT) columns were tested under concentric compression loads. The effects of the replacement ratio of the recycled concrete aggregate (RCA) and steel fibre (SF) reinforcement on the structural performance of the SCRCFAT columns were studied. A control specimen (C000) was cast with normal concrete without SF to be reference for comparison. Twelve columns were cast using RCA, six columns were cast using concrete incorporating 2% SF while the rest of columns were cast without SF. Failure mode, ductility, ultimate load capacity, axial deformation, ultimate strains, stress-strain response, and stiffness of the SCRCFAT columns were studied. The results showed that, the peak load of tested SCRCFAT columns incorporating 5-100 % RCA without SF reduced by 2.33-11.28 % compared to that of C000. Conversely, the peak load of tested SCRCFAT columns incorporating 5-100% RCA in addition to 2% SF increased by 21.1-40.25%, compared to C000. Consequently, the ultimate axial deformation (Δ) of column C100 (RCA=100% and SF 0%) increased by about 118.9 % compared to C000. The addition of 2% SF to the concrete mix decreased the axial deformation of SCRCFAT columns compared to those cast with 0% SF. Moreover, the stiffness of the columns cast without SF decreased as the RCA % increased. In contrast, the columns stiffness cast with 2% SF increased by 26.28-89.7 % over that of C000. Finally, a theoretical model was proposed to predict the ultimate loads tested SCRCFAT columns and the obtained theoretical results agreed well with the experimental results.

• Journal of the Korea Concrete Institute
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• v.17 no.2 s.86
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• pp.263-271
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• 2005

HPFRCC(High Performance Fiber Reinforced Cementitious Composite) is a class of FRCCs(Fiber Reinforced Cementitious Composites) that exhibit multiple cracking. Multiple cracking leads to improvement in properties such as ductility, toughness, fracture energy, strain hardening, strain capacity, and deformation capacity under tension, compression, and bending. These improved properties of HPFRCCs have triggered unique and versatile structural applications, including damage reduction, damage tolerance, energy absorption, crack distribution, deformation compatibility, and delamination resistance. These mechanical properties of HPFRCCs become different from the kinds and shapes of used fiber, and it is known that the effective size of fiber in macro crack is different from that in micro crack. This paper reports an experimental findings on the mechanical properties of HPFRCCs reinforced with the micro fiber(PP50, PVA100 and PVA200) and macro fiber(PVA660, SF500). Uniaxial compressive tests and three point bending tests are carried out in order to compare with the mechanical properties of HPFRCCs reinforced with micro fibers or hybrid fibers such as compressive strength, ultimate bending stress, toughness, deformation capacity and crack pattern under bending, etc.,

• Nutrition Research and Practice
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• v.18 no.2
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• pp.257-268
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• 2024

BACKGROUND/OBJECTIVES: This study investigated the relationship between adherence to the Mediterranean diet among Korean baby boomers and their levels of psychosocial stress. SUBJECTS/METHODS: The study included 1,656 adults (889 men and 797 women) born between 1955 and 1963 who participated in the 2005-2006 survey of the community-based Korean Genome and Epidemiology Study (KoGES). The Mediterranean-type diet score (MTDS) was calculated from the semi-quantitative food frequency questionnaire (SQFFQ) data. The psychosocial stress levels were calculated using the psychosocial well-being indexshort form (PWI-SF). Logistic regression analyses were performed to analyze the association between the MTDS (tertiles) and the prevalence of high psychosocial stress by gender. RESULTS: The ranges of the MTDS tertile groups were T1 (20-33 points), T2 (34-37 points), and T3 (38-39 points) for men, T1 (20-33 points), T2 (34-37 points), and T3 (38-48 points) for women. In both men and women, the consumption of whole grains, potatoes, fruits, vegetables, legumes, and fish increased with higher MTDS, while the consumption of red meat and dairy products decreased (P for trend < 0.05). As MTDS score increased the intake of energy, fiber, vitamins, and minerals (P for trend < 0.05). Men in the highest MTDS tertile had a 41% lower odds ratio (OR) of high psychosocial stress compared with those in the lowest tertile (OR, 0.59; 95% confidence interval [CI], 0.38-0.91). Similarly, women in the highest tertile of the MTDS had a 39% lower OR of high psychosocial stress compared with those in the lowest tertile (OR, 0.61; 95% CI, 0.40-0.95). CONCLUSION: Promoting adherence to the Mediterranean diet among baby boomers may have a positive impact on reducing their levels of psychosocial stress.