• Title/Summary/Keyword: SEX DETERMINATION

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Use of the Non-electrophoretic Method to Detect Testis Specific Protein Gene for Sexing in Preimplantation Bovine Embryos

  • Huang, Jinming;You, Wei;Wu, Naike;Tan, Xiuwen
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.6
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    • pp.866-871
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    • 2007
  • Testis-specific protein (TSPY) is a Y-specific gene, with up to 200 copy numbers in bulls. In order to make bovine embryo sexing under farm condition more feasible, the possibility of using a non-electrophoretic method to detect the TSPY gene for sexing bovine early embryos was examined. Primers were designed to amplify a portion of the TSPY gene and a common gene as an internal control primer. PCR optimization was carried out using a DNA template from bovine whole blood. Furthermore, embryo samples were diagnosed by this method and the sexing results were contrasted with those of the Loop-Mediated Isothermal Amplification (LAMP) method. The results showed that TSPY was as reliable a sexing method as LAMP. Forty-three morula and blastocyst embryos collected from superovulated donor dairy cattle were sexed by this method, and twenty-one embryos judged to be female embryos were transferred non-surgically to recipients 6 to 8 days after natural estrus. Out of 21 recipients, 9 were pregnant (42.86%) and all delivered female calves. The results showed that the sex predicted by this protocol was 100% accurate. In conclusion, the TSPY gene was a good male specific marker and indicated that a non-electrophoretic method was feasible and accurate to detect the TSPY gene for sexing preimplantation bovine embryos.

An Integrative Review of Sexuality and Mental Health (성(Sexuality)과 정신건강에 관한 통합적 문헌고찰)

  • Kim, Jandi;Ryu, Jae Geum;Oh, Sangjun;Shin, Youjin
    • Perspectives in Nursing Science
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    • v.18 no.1
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    • pp.16-27
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    • 2021
  • Purpose: Sexuality is a fundamental element of human life and is closely related to mental health. This study was conducted to understand domestic research trends on gender, mental health, and psychological well-being through an integrated review. Methods: Through four domestic databases, 15 papers were finally selected using the search terms 'sex', 'mental health', and 'psychological well-being'. Results: Fifteen papers have been published since 2000 and 10 documents have been published since 2010. A majority of the studies were basic research and reviews, and these included four qualitative studies. Depending on the subject, we classified five articles into three themes by the developmental stages (youth, married women, and the elderly), eight sexual minority studies, and two other studies (right to sexual self-determination, and dating for people with disabilities). Conclusion: A more open social concern and approach to sex, the most basic human need, is warranted. The field of nursing practice requires more attention.

Eating Styles and Food Consumption Behaviors of College Students (대학생들의 섭식유형과 음식 소비행동)

  • Suh, Seong-Mu
    • Journal of the Korean Society of Food Culture
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    • v.23 no.2
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    • pp.214-221
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    • 2008
  • This study analyzed the survey results of 302 college students from a coed university in Korea. The following three research components were examined: relationships among different eating styles, violation behaviors after unwanted eating, and consumption behaviors for three different food types. The analysis results showed that restrained eating was positively related to emotional eating, but negatively related to external eating. And emotional and external eating displayed a positive relationship. Body shape dissatisfaction was a significant variable in explaining restrained eating behavior. After unwanted eating, the restrained eaters expressed more regrets and stronger determination to diet than the less restrained eaters. The emotional eaters and external eaters responded that they could not stop eating and performed binge eating behaviors when they failed their diet. With regard to food attitudes and eating styles, snacks were favorably related to emotional eating. Fast food attitudes were negatively associated with restrained eating. Preference was the only significant variable in explaining snack consumption frequency; however sex, preference, and restrained eating were significant for fast foods. Finally, vegetarian foods were explained by preference and sex.

DNA barcoding of Raptor carcass collected in the Paju city, Korea (파주시에서 수집한 폐사체 맹금류의 DNA 바코드 연구)

  • Jin, Seon-Deok;Paik, In-Hwan;Lee, Soo-Young;Han, Gap-Soo;Yu, Jae-Pyoung;Paek, Woon-Kee
    • Korean Journal of Environment and Ecology
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    • v.28 no.5
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    • pp.523-530
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    • 2014
  • One juvenile raptor which was not able to be identified due to its head damage was discovered on a roadside in Janggok-ri, Jori-eup, Paju on 28th June, 2011. The species was identified by DNA barcoding. After polymerase chain reaction (PCR) of the mitochondrial cytochrome c oxidase subunit I gene (COI), we obtained 695 bp sequences. We analyzed the obtained COI sequence with similar sequences from the BOLD systems and BLAST of the NCBI Genbank, and discovered that its sequence showed 100 % similarity values with the one of the five gray-faced buzzards which were previously researched. In addition, it was confirmed to be a female through sex determination using DNA. Such results are important information as it confirms the breeding of the gray-faced buzzards for the first time in 43 years since its breeding was last recorded in 1968, in Paju. Wildlife rescue center needs to work with adjacent consigned registration and preservation institutions when carcass of wild animals is collected or DNA samples are obtained for more accurate both species and sex identification through a systematic management system in the future. Furthermore, the obtained DNA sample of the gray-faced buzzard and COI gene, DNA barcode, could be used as reference standards for similar researches in the future.

Teachers' Needs Analysis about Contents of Sexual Education for the Lower Grades of Primary School (초등학교 저학년 성교육 내용에 대한 교사의 요구 조사)

  • Jeong, Geum-Hee;Yang, Soon-Ok;Kim, Shin-Jeong
    • Women's Health Nursing
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    • v.13 no.1
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    • pp.35-42
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    • 2007
  • Purpose: This study was done to discover the contents needed for a sexual education program in the lower grades (the first to the third grade) by primary school teachers and to discover difficulties in confronting sexual education. Method: This was a descriptive study of 198 teachers for lower grade students in 15 primary schools. A questionnaire on sexual education was distributed and collected by mail. Result: The contents for sexual education that teachers felt were highly needed are as follows: sanitation of genitals, sexual violence, pregnancy & childbirth, family, selfassertion, and sexual self-determination. Teachers think prevention of sexual violence is the main reason for sexual education. Teachers indicated that sexual education considering the developmental stage was difficult. Conclusion: Development of a sexual education curriculum for lower grade primary school students is necessary for increasing the problem-solving ability of students focused on sexual violence, self-assertion and sexual self-determination, in addition to providing simple information.

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Studies on Separation and Determination of Korean Bovine Serum Protein by Colorimetric Method (비색법에 의한 한우 혈청단백질의 분획정량 시험)

  • Cho, J.H.
    • Korean Journal of Veterinary Research
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    • v.11 no.2
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    • pp.145-148
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    • 1971
  • Serum Samples from adult of Korean cattles including 40 females and 20 males were analyzed by sodium salt precipitation and colorimetric method in the purpose of the determination of total serum protein, albumin, globulin, ${\alpha}$-globulin, ${\beta}$-globulin and ${\gamma}$-globulin. The results obtained arc summarized as follows: 1. Mean value of total serum protein showed a slight variation from 7.6%, and its regional and sex differences were not found to be significant. 2. Contents of albumin in serum showed lower level than that of globulin as low level of A/G ratio 0.4 in proportion. 3. Contents of Serum ${\alpha}$-globulin showed 1.4w/v% and $1.51{\pm}0.46$w/v% in each group of female, and $1.31{\pm}0.26$w/v%, in the group of male. 4. Contents of serum ${\beta}$-globulin showed 1.74w/v%, 1.95w/v%, in each group of female, and 1.82w/v% in the group of male. 5. Contents of serum ${\gamma}$-globulin showed 2.32w/v%, 2.30w/v% in each group of female, and 2.30w/v%, in the group of male.

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Molecular Cloning and Expression of DMRT Gene in Protogynous Wrasse, Halichoeres tenuispinis

  • Jeong, Hyung-Bok;Park, Ji-Gweon;Park, Jin-Young;Jin, Young-Jun;Yang, Myung-Cheon;Hyun, Kyung-Man;Kim, Gi-Ok;Kim, Se-Jae
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2003.10a
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    • pp.64-64
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    • 2003
  • The sex differentiation of fishes occurs under the control of genetic and various environmental factors. DM-domain containing genes are novel zinc finger transcription factors and play key roles in sex determination. In order to isolate the wrasse DMRT (wDMRT) cDNA from the protogynous wrasse (Halichoeres tenuispinnis), the wrasse testis cDNA library was screened using the $^{32}$ P-labeled PCR products, which were amplified with the degenerate primers from conserved DM-domain regions of several DMRT genes. Among a few positives obtained through screening, the full length wDMRT cDNA of 2.9kb size encoding a predicted 300 amino acid residues was isolated. The sequence analysis exhibited 60%, 43% sequence identity with rainbow trout and tilapia DMRT1, respectively. RT-PCR assay showed that wDMRT was expressed specifically in male testis. Also, wDMRT gene was strongly expressed in May during reproductive season, when the reproductivity of wrasse is most active. This results suggested that wDMRT gene function in testis differentiation The conserved DM-domain regions were amplified using PCR from DMRT genes of several species among Labridae, and their sequences were determined. The sequence of DM-domain region of Halichoeres. tenuispinis was identical to those of Pseudolabrus japonicus, Pteragogus flagellifera, and showed 94% identity with that of Halichoeres poecioptrerus.

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Methoxychlor Produces Many Adverse Effects on Male Reproductive System, Kidney and Liver by Binding to Oestrogen Receptors

  • Kim, Dae Young
    • Journal of Embryo Transfer
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    • v.28 no.2
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    • pp.157-162
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    • 2013
  • Methoxychlor (MXC) was developed to be a replacement for the banned pesticide DDT. HPTE [2,2-bis (p-hydroxyphenyl)-1,1,1-trichloroethane], which is an in vivo metabolite of MXC, has strong oestrogenic and anti-androgenic effects. MXC and HPTE are thought to produce potentially adverse effects by acting through oestrogen and androgen receptors. Of the two, HPTE binds to sex-steroid receptors with greater affinity, and it inhibits testosterone biosynthesis in Leydig cells by inhibiting cholesterol side-chain cleavage enzyme activity and cholesterol utilisation. In a previous study, MXC was shown to induce Leydig cell apoptosis by decreasing testosterone concentrations. I focused on the effects of MXC on male mice that resulted from interactions with sex-steroid hormone receptors. Sex-steroid hormones affect other organs including the kidney and liver. Accordingly, I hypothesised that MXC can act through sex-steroid receptors to produce adverse effects on the testis, kidney and liver, and I designed our experiments to confirm the different effects of MXC exposure on the male reproductive system, kidney and liver. In these experiments, I used pre-pubescent ICR mice; the puberty period in ICR mice is from postnatal day (PND) 45 to PND60. I treated the experimental group with 0, 100, 200, 400 mg MXC/kg b.w. delivered by an intra-peritoneal injection with sesame oil used as vehicle for 4 weeks. At the end of the experiment, the mice were sacrificed under anaesthesia. The testes and accessory reproductive organs were collected, weighed and prepared for histological investigation. I performed a chemiluminescence immune assay to observe the serum levels of testosterone, LH and FSH. Blood biochemical determination was also performed to check for other effects. There were no significant differences in our histological observations or relative organ weights. Serum testosterone levels were decreased in a dose-dependent manner; a greater dose resulted in the production of less testosterone. Compared to the control group, testosterone concentrations differed in the 200 and 400 mg/kg dosage groups. In conclusion, I observed markedly negative effects of MXC exposure on testosterone concentrations in pre-pubescent male mice. From our biochemical determinations, I observed some changes that indicate renal and hepatic failure. Together, these data suggest that MXC produces adverse effects on the reproductive system, kidney and liver.

Flower bud development and the influence of sex expression of summer pruning in Schizandra chinensis Baillon (오미자의 화아(花芽) 발달과 성 발현에 미치는 하계전정의 영향)

  • Kim, Sun;Kim, Tai-Su;Park, Moon-Soo;Park, Ho-Ki
    • Korean Journal of Medicinal Crop Science
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    • v.11 no.2
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    • pp.97-101
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    • 2003
  • The purpose of this experiment is to improve the ratio of female flower in Schizandra chinensis Baillon. The experiment was carried out in two purposes. One was to understand the development stages on bud formation and determining sex after bud formation by stage. The other was to investigate characteristics of flowers forming on spring when summer pruning was taken place in different cutting part and timing in S. chinensis Baillon. The outcome was that on 7th of June the flower bud was found next to leaf primordium in branch of this year and no changes were found in shape for two weeks by 26th July. On 26th of June, an ovule that forms organ was observed and then, an ovule was found after sex was determined on 6th of July. Second experiment was carried out to find out when the sex was determined by adapting different pruning methods; cutting the branch at 6th node on June 1, June 15, and July 1; cutting the branch at 10th node on June 15, and July 1. The result was that the re-growth ratio on the branch 6th node was cut on June 1 and June 15. July 1 was 34-56% and, however, the number of setting flowers and mail flowers were fewer than the normal branches. More over, even though the re-growth ratio of branches were 34-25% when the cutting was taken place at 10th node on June 15 and July 1, the number of setting flowers and mail flowers were the same as the non treated branch. As a result, it is assumed that cutting controlling in this plant to increase the number of female flowers has no effect.

Studies on the Hematology and Blood Chemistry of Korean Cattle Part I. Studies on the Hematology of Korean Cattle (한국성우(韓國成牛)의 혈액학치(血液學値) 및 혈액화학치(血液化學値)에 관한 연구(硏究) 제1보(第一報) 한국성우(韓國成牛)의 혈액학치(血液學値)에 관한 연구(硏究))

  • Cheong, Chang Kook
    • Korean Journal of Veterinary Research
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    • v.5 no.1
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    • pp.61-96
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    • 1965
  • Observations were made on the blood picture of total 196 heads of healthy Korean cattles, including 98 males and 98 females in the purpose of determination of hematological values and its sex difference, and seasonal variations during one year period from December 1963 to November 1964. The blood sampling were scheduled by random in four different seasons and the sample size of both sex included in each season were designated to be same size. The ranges, averages or mean values of the erythrocytes, hemoglobin, hematocrit, mean corpuscular hemoglobin concentration, total white blood cell count and differential count were determined in this studies and their respective standard deviation, standard error of means, sex defferences and seasonal variations were as follows; 1. The erythrocyte count of male showed a range of $5.0{\times}10^6/c.mm$ to $8.75{\times}10^6/c.mm$ with a mean of $6.5{\pm}0.096{\times}10^6/c.mm$. Female showed a range of $5.0{\times}10^6/c.mm$ to $8.30{\times}10^6/c.mm$, with a mean of 6. $131{\pm}0.078{\times}10^6/c.mm$. There was a highly significant sex difference and seasomal variation was not found to be significant. 2. The hemoglobin value of male showed a range of 9.0g/100cc. to 14.5g/100cc. with a mean of $11.074{\pm}0.143g/100cc$. Female showed a range of 9.0g/100cc to 13.0g/100cc. with a mean of $10.745{\pm}0.034g/100cc$. There was a highly significant sex difference and seasonal variation was not found to be significant. 3. The hematocrit value of male showed a range of 28% to 45% and with a mean of $34.867{\pm}0.468%$. Female showed a range of 28% to 42% with a mean of $32.888{\pm}0.322%$. There was a highly significant sex difference and seasonal variation was not found to be significant. 4. The mean corpuscular hemoglobin of male showed a range of 14.4rr. to 19.6rr. with a mean of $17.1{\pm}0.112rr$. Female showed a range of 14.7rr. to 19.5rr. with a mean of $17.6{\pm}0.113rr$. 5. The mean corpusular volume of male showed a range of $42.5{\mu}^3$ to $62.2{\mu}^3$ with a mean of $53.9{\pm}0.419{\mu}^3$, Female showed a range of $44.2{\mu}^3$ to $60.0{\mu}^3$ with a mean of $53.8{\pm}0.375{\mu}^3$. 6. The mean corpuscular hemoglobin concentration of male showed a range of 28.1 % to 34.9% with a mean of $31.4{\pm}0.161%$. Female showed a range of 28.0% to 34.9% with a mean of $30.9{\pm}0.169%$. 7. The total leucocyte count of male showed a range of 4,000/c.mm to 13,100/c.mm. with a mean of $9,338{\pm}218.23/c.mm$. Female showed a range of 4,000/c.mm. to 14,000/c.mm. with a mean of $9,338{\pm}235.90/c.mm$. Six difference was not found to be significant and there was a highly significant seasonal variation. 8. The differential count of male, the means of neutrophil, stab, segmented cell, Iymphocyte, monocyte, eosinophil and basophil were $31.173{\pm}0.570%$, 0.3%. $30.867{\pm}0.564%$, $55.112{\pm}0.603%$, $3.745{\pm}0.082%$, $9.867{\pm}0.422%$ and 0.14% rspectively. Female showed means of $31.010{\pm}0.572%$, 0.2%, $30.806{\pm}0.569%$, $53.929{\pm}0.634%$, $4.082{\pm}0.109%$, $10.908{\pm}0.503%$ and 0.12% respectively. There were significant sex differences in monocyte and highly significant sex difference in eosinophil, and seasonal variation were found to be highly significant in neutrophil, monocyte and eosinophil. 9. Hematological comparison made between cattles infested with so called "small type piroplasma" and non-infested group. The result of investigation showed no significant difference upon the red blood cell, hemoglobin and hematocrit values between lighty infested group and non-infested group. 10. Age distribution of test group in this study ranged from 2 years to 6 years in both sex and their average age were $4.45{\pm}0.114$(male) and $4.50{\pm}0.116$(female). There found to be no significant sex difference and seasonal variations in the age of test group.

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