• Horticultural Science & Technology
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• v.18 no.4
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• pp.508-512
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• 2000

Growth of red pepper (Capsicum annuum) 'Kumtap', tomato (Lycoperisocon esculentum) 'Seokwang', petunia (Petunia hybrida) 'Madness Rose', and pansy (Viola tricolor) 'Magestic Giant' in mixtures of chestnut woodchips and granular rockwool at 25:75, 50:50, or 75:25 (v/v) was examined. Chestnut woodchips were soaked in water for 48 hours or aged in open field for 6 months in order to remove substances impeding plant growth. A commercial plug medium was used as the control. All treatments showed in a similar result in red pepper, petunia, and pansy. Plant height, fresh weight, dry weight, and chlorophyll content in media containing chestnut woodchips, especially in higher proportions, were poorer as compared to those in the control. On the contrary, height, fresh weight, and dry weight of tomato seedlings in media containing woodchips were significantly higher than those in the control. For petunia, pansy, and red pepper, six month ageing in open field of woodchips was more favorable for growth than 48 hour water soaking. Emergance of petunia seed was inferior, especially when woodchip content was higher, to the other crops with a resultant growth suppression. From the results, chestnut woodchips proved to be a practical material as a medium component only in tomato plants.

• Journal of Bio-Environment Control
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• v.14 no.4
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• pp.302-306
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• 2005

This Study was Conducted in 3 glasshouse to examine the Possibility for cultivation of plug seedlings using four growing media containing recycled PET-bottle-particles (PBT) as a medium component. Plug seedlings of 'Seokwang' tomato were grown in media containing $100\%$ PBT, $100\%$ peatmoss, or $50\%$ $PBT+50\%$ peatmoss. A commercial plug medium (Tosilee) was used as the control. All seeds were sown in 128 cell plug trays on July 12, 2004. Seedling growth was measured at 30 days after sowing. Percent seed germination was greater than 95% in all treatments. Plant height, shoot fresh and dry weights, leaf area, and hypocotyl length were the greatest in the control treatment. Root length and weight were the greatest in the $100\%$ PBT treatment, which had a medium pH of 7.3. Leaf area and shoot dry weight were greater in the $50\%$ $PBT+50\%$ peatmoss treatment, than in the $100\%$ PBT treatment, while they were the smallest in the $100\%$ peatmoss treatment. No toxicity symptoms caused by the use or PBT had been observed in any treatment. The results suggest a possibility of recycled PBT, a valuable reusable resource, to be used as a component for medium amendments in horticultural transplant production.

• Korean Journal of Plant Resources
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• v.29 no.1
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• pp.128-135
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• 2016

This study examined radiation damage and the optimal gamma-ray dose for mutation breeding in oat (Avena sativa L. cv. Samhan). The seed germination rate decreased as the dose increased over 500 Gy. The median lethal dose (LD₅₀) was approximately 392 Gy. The median reduction dose (RD₅₀) for plant height, tiller number, root length, and flash weight was 411, 403, 394, and 411 Gy, respectively. The optimal dose of gamma irradiation for inducing oat mutation appears to be in the range 300-400 Gy. We performed the comet assay to observe nuclear DNA damage induced by gamma-ray irradiation. This assay showed a clear difference with gamma-ray treatments. DNA damage increased temporarily 7 days after treatment depending on the dose, while no significant difference was identified in response to 300 Gy 30 days after the gamma-ray treatments. The growth characteristics of the M₂ generation decreased as the dose increased over 400 Gy.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.99-99
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• 2018

본 연구는 4종의 산야초 추출물(개느삼, 엄나무, 참취, 60가지 산야초혼합 - 모두 원 식물재료를 설탕과 혼합하여 추출한 발효추출물을 15년 숙성시킨 원액의 3% 수용액으로 처리)과 8종의 허브 정유(베르가모트, 클라리세이지, 휀넬, 제라니움, 마조람, 타임, 로즈우드, 스페어민트 원액의 0.3% 농도로 처리) 및 2종의 호르몬(지베렐린, 카이네틴, 0.3%)을 2종의 보리종자(1998년산 새올보리, 2016년산 찰쌀보리) 발아율 향상에 영향을 미치는지를 알아보고자 수행하였다. 특히 새올보리 묵은 종자의 발아력 증진에 효과가 있는지 알아볼 목적으로 수행하였다. 묵은 종자 새올보리의 무처리구에서 8%와 43%의 낮은 발아세와 발아율을 보인 반면, 산야초 추출물 처리구에서 84~93%와 86~100%의 높은 발아세와 발아율을 보였다. 호르몬처리구는 새올보리에서 발아세와 발아율은 각각 43~87%와 55~95%였다. 또한, 찰쌀보리의 경우 무처리의 발아세와 발아율은 각각 54%와 91%였다. 반면, 산야초 추출물 처리구에서 92~100%의 발아세와 95~100%의 높은 발아율을 보였고, 호르몬 처리구에서는 각각 77~85%와 87%의 발아세와 발아율을 보였다. 무처리(증류수)와 2종의 호르몬 및 8종의 허브 정유에 비해 산야초 추출물의 발아 증진 효과가 현저하게 높았다. 특히 냉장고($4^{\circ}C$)에서 20년 보관한 묵은 보리종자에 대해서도 유의한 발아율 향상을 보였다. 정유는 대체로 발아를 억제하는 것으로 나타나 잡초 종자에 대한 발아억제(제초제 대용) 가능성을 시사한다.

• KSBB Journal
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• v.16 no.4
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• pp.344-350
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• 2001

The effect of salinity stress of Brassica olearacea and Capsicum annuum were studied at various levels of salinity conditions(Na-gluconate, K-gluconate, NaCl, KCl). The effects of salinity stress were measured by seedling growth rates and secondary metabolites contents of the stressed plants. Each seedling studied on the response of different salinity stress. Seedling growth of Capsicum annuum was inhibited up to 200 mM salt tolerance and Brassica olearacea was inhibited up to 400 mM salt tolerance. The produced anthocyanin was separated to high value from 200 mM NaCl in case of Brassica olearana and 50 mM K-gluconate in case of Capsicum annuum. The BADH activity was very high in Brassica olearacea seedlings treated with 200 mM NaCl and in Capsicum annuum seedlings treated with 100 mM K-gluconate. The BADH activities were increased during the early culture days, it induced betaine synthesis. The salinity stress promoted BADH activiy, subsequently endogenous betaine contents were increased, and it seemed to be secure seedling from salinity stress. The salinity concentration of 200 mM was effective on the inhibition of seed germination and on the increase of proline accumulation in tissue. The inhibition of seedling growth and accumulation of secondary metabolites in seedling were caused osmotic hypersensitivity against salinity stress.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.2
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• pp.241-246
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• 2004

To develop novel anti-aging peptides from the germinated black rice, we treated with bromelain, papain and Pronase E. And we investigated the effects of the germinated black rice peptide (GBRP) as anti-aging cosmetic ingredients, and compared with the non-germinated black rice protein (NBRP). We investigated the effects on in vitro inhibition of matrix-metalloprotease (MMP), proliferation of human skin fibroblasts, stimulation of collagen synthesis and expression of UVA-induced MMPs in human skin fibroblasts, UVA induced MMP-1 expression and collagen contents in human skin fibroblasts were analyzed by enzyme-linked immunosorbent assay (ELISA). As a result, the molecular weight distributions of GBRP and NBRP were determined by gel permeation chromatography to be approximately 900 and 10,000 daltons. GBRP increased skin cell proliferation about 40% and reduced UVA-induced MMP-1 expression about 50%. Also the collagen protein level of cells, which were cultured with GBRP, was increased about 25%. These results suggest that the geminated plant seed peptides can be novel anti-aging ingredients for cosmetics.

• Korean Journal of Plant Resources
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• v.21 no.4
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• pp.304-309
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• 2008

All the seeds of rape (Brassica napus) sprout vegetables were germinated within three days after seeding irrelevant to the light colors. The total fresh weight of rape sprout vegetables at 6 days after seeding have been increased when grown under green and white color lights by 0.339g/10plants and 0.339g/10plants compared with the control. The total flavonoid contents in rape sprouts were increased under red and blue lights by 72.5 and 70.9mg${\cdot}L^{-1}$. Those DPPH radical scavenging activities at 2,000mg${\cdot}L^{-1}$ were increased by 90.0 and 90.3% which were sprouted and grown under blue and white lights. Nitrite radical scavenging activity of sprouts were most decreased compared with the control when grown under the red light by 57.4mg${\cdot}L^{-1}$. And mushroom tyrosinase inhibition activity of rape sprouts was extremely increased under the green light by 22.5mg${\cdot}L^{-1}$.

• Molecules and Cells
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• v.40 no.3
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• pp.230-242
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• 2017

In the Arabidopsis genome, approximately 80 MAP3Ks (mitogen-activated protein kinase kinase kinases) have been identified. However, only a few of them have been characterized, and the functions of most MAP3Ks are largely unknown. In this paper, we report the function of MAP3K16 and several other MAP3Ks, MAP3K14/15/17/18, whose expression is salt-inducible. We prepared MAP3K16 overexpression (OX) lines and analyzed their phenotypes. The result showed that the transgenic plants were ABA-insensitive during seed germination and cotyledon greening stage but their root growth was ABA-hypersensitive. The OX lines were more susceptible to water-deficit condition at later growth stage in soil. A MAP3K16 knockout (KO) line, on the other hand, exhibited opposite phenotypes. In similar transgenic analyses, we found that MAP3K14/15/17/18 OX and KO lines displayed similar phenotypes to those of MA3K16, suggesting the functional redundancy among them. MAP3K16 possesses in vitro kinase activity, and we carried out two-hybrid analyses to identify MAP3K16 substrates. Our results indicate that MAP3K16 interacts with MKK3 and the negative regulator of ABA response, ABR1, in yeast. Furthermore, MAP3K16 recombinant protein could phosphorylate MKK3 and ABR1, suggesting that they might be MAP3K16 substrates. Collectively, our results demonstrate that MAP3K16 and MAP3K14/15/17/18 are involved in ABA response, playing negative or positive roles depending on developmental stage and that MAP3K16 may function via MKK3 and ABR1.

• Weed & Turfgrass Science
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• v.4 no.2
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• pp.118-123
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• 2015

Metagenomics is a powerful tool to isolate novel biocatalyst and biomolecules directly from the environmental DNA libraries. Since the metagenomics approach bypasses cultivation of microorganisms, un-cultured microorganisms that are majority of exists can be the richest reservoir for natural products discovery. To discover novel herbicidal substances from soil metagenome, we established three easy, simple and effective high throughput screening methods such as cucumber cotyledon leaf disc assay, microalgae assay and seed germination assay. Employing the methods, we isolated two active single clones (9-G1 and 9-G12) expressing herbicidal activity which whitened leaf discs, inhibited growth of microalgae and inhibited root growth of germinated Arabidopsis seeds. Spraying butanol fraction of the isolated active clones' culture broth led to growth retardation or desiccation of Digitalia sanguinalis (L) Scop. in vivo. These results represent that the screening methods established in this study are useful to screen herbicidal substances from metagenome libraries. Further identifying molecular structure of the herbicidal active substances and analyzing gene clusters encoding synthesis systems for the active substances are in progress.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.15 no.1
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• pp.141-154
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• 2012

This study was launched to verify the effective composition of plant species and its management program most suited for the dredged soiled area near Nakdong River Site 14. The improvement methods of planting base and the composition of plants such as silver grass, reed, and some colonies in aesthetic effect were studied. To search the management methods to decrease the confining pressure risen from the burried seeds which would consequently harm the previously seeded plants, experimental construction process was measured on the site. The purpose of this experiment was to figure out which part of the improvement on the plant base has the most significant effect for the revegetation of infertile, dredged soil, to verify the easily seeded, developing plants among seeded plants, and finally, to find the restoration model using plants near the dredged soil around riverside. 8 seeded plants and 23 invaded species were appeared which among the emerged plants, development of Aster yomena MAKINO, Lotus corniculatus var. japonica Regel, Trifolium repens L, and Dianthus longicalyx Miq were proved to be brought up well. Difference risen from the seed composition were not noticeable until 150day since the germination was proceeded mainly by Aster yomena MAKINO. The experimental plot with dredging sand+organic fertilizer method of construction and dredging sand+soil conditioner method showed most development while the effect of the plot with only the soil base of dredging sand stayed low. Another important method for the management of infertile, dredged soil base would be the removal of disturbing species which the experiment showed the tied relationship between the removal of disturbing species and development of seeding plants. Although this study was carried out focused on the Nakdong River Project, the study suggests the general management program that the removal of disturbing species such as Humulus japonicus Sieboid & Zucc. and Pueraria lobata (Willd.) Ohwi in times around rainy season(60days after seedling) would be effective for the easy growth of revegetation plants.